Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
FLH203009.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pDONR221
Format:
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
ScCD00025160
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4740nts
Open reading frame: 1 to 4740
ATGTCGCATTCAGACTACTTCAATTATAAGCCTTACGGCGATTCCACGGAAAAGCCCAGC
AGCTCCAAGATGAGGCAGTCATCTTCATCATCTTCATCTAGACTAAGGTCGGAAAGTTTA
GGGCGAAATTCCAATACTACGCAGGCTCGAGTAGCGTCATCGCCCATCAGCCCAGGGCTG
CATTCTACCCAGTACTTTAGATCACCAAACGCTGTTTACAGCCCTGGAGAGTCTCCATTA
AATACAGTACAGCTATTCAATCGTCTTCCGGGTATACCTCAAGGTCAGTTTTTTCATCAA
AATGCCATTTCTGGGTCCTCCAGCTCCTCGGCAAGATCTAGTAGACGGCCCTCGAATATT
GGTCTTCCTCTACCAAAAAATCCTCAGCAGTCTCTACCGAAGTTGTCTACTCAACCTGTT
CCTGTACATAAGAAAGTTGAGGCTAGTAAAACAGAGAGCGAGATTATTAAGAAGCCCGCT
CCCGTGAATAGTAATCAAGATCCATTATTGACGACCCCAACGTTAGTGATATCACCAGAA
CTGGCTTCACTAAATACAACGAATACATCGATTATGTCAACGCCACAAAATATTACAAAC
CAAACTTCGAACAAACACATTCCCACCAGATCGCAACCAAATGGGTCAACAAGTTCCTCC
ACTTTGCAGGATATTGTCACGACAAATAGCTCGCAACGGTCTGTAGGCCACCATGGTGGA
AGCACAACGAGCCTCCGAACATACAAAAAACAATATGTATTAAATGAACAGTTATATTTA
AGAAAAATGAGAAACCGTGCTAATGATGATTATTACACTAGAGGTATAGTCGCATCATCC
AACTTTGAAGATGACGAAGAAAATTTTAGTAACAAAGGTGAAGATGACTTAGAACTAGAA
ATGGATGATCTTTTAAAGGTAGAAGGTGAGGATAAAGATAACGACTTCAATTTTGGTTAT
AATTTTATTACGTCGAGCACAAAAAATAATGAAAATGTTGTTTCGATGAGCCTAAATTAT
CTAAAGGGCAAATTGGATTGGTTGAGGGATGTGAACAATGATCAACCGTGTGAAATAGAA
GATGAGGAGTGGCATTCCATACTGGGGAGCGAGGATTTGCTGTCAAAATTGTTACAAAAT
CCTATGGTGAACAACCGATTTGAATGGCAAACAATGTTATCTAAGGTACTGAAGGGAGAT
ATTGTGAGGAATGAAAAAACGAAGATTGCAAATCAAGGGAAAGGCCCTGGCTTCAATACT
CAGTTTTCAGATGACATTTGGATTGAGTTGAAGGCATGGATGAATGGGAGGACCGTGGAA
GATCAGAACAAATCTCTGAGGATTTTTAGGGATTCTACTGACTCCGTATTTCAAGAAATC
ATGGCCTTTAAACTAGAAGATAATATGAGCGCTGACGAGGCTGCAGAGACTATCAAATCA
CTAGTAGACAAATATTATAGAGTCTTAAATCTATGGCCTAACATTAAAAGAATGCATGCT
GAAAAACCCATTACTAAAACAGAAGCATTCAGGAATCGAATAGATACTTTGAATAGTTGG
CTTAATTTCAAATTTAACTTTGATACTAATATTGCGTACCTGAAAAAATGGATAGTTGGC
AATAAAGAGCTAGAAAGCACTACCGAAGTGGATAACACCACCGTGAATTTGGATGATCCA
GCCGTTTTCGCCACTAATTGTAAACGCTTTGCGGAGCAAATTATGAAGGAAAAGGATATT
GAACTGATATTTCAAAAAAAAATATTCTTTCCATTAGCACCATGGATTTTGAAGGCCAAA
TTTTTCTTCTTGAAATACCAAAAAACTTGGAATGAATTGAATCTATCTTATTTGGATCAA
GATCTGGAATTTTTATTGATGTTTCCAATGCGTTTGGTAAAAGATATAATACTAATTCGC
CTATCTTACGCGAAGAAAATACAGAATCCAACCTTGATGATGATCGATCAAATGATGGAC
GATTTTAGTACATATATTAAGTTGGCAGTCCAAATGAAATTCACTGTTGCTTCTTACTGT
AATGACTGGTTTTTTAAAGTGAAAATCGATCCCGAATTTGATCATACCGTTGTTGAAGGA
TTGGAATATTTTTTCTCCATTTTGGAACTGAGAATATTATATAGTGGCAAAAACTCATTC
AAGACTTCTAAAGAACCTGATCTGTTATTAAAATATTGGGAAATGTTTAGAAACGTCGGC
TATTATATTGATGATGCAGGCGAACTGATCGCAGCAGAATTTACGAAGTTGACACTTAGA
TTGGTTCACAGATTGCACGCTTACCTTTTAAGGCAGCAAAACACTCCGCCAAAATTAGAG
AATGAAGCTGCTGCTGAAAAATGGCTGGTGCAAATATTCGAAATACTTGGATCCATGAAA
AGAAAACTCAATAGATTCACCAATATTTTGACAAAAGCATTTCAAAATTTTGTCCGCTAC
AAGATAGAAGATCACAACTATCTGCTAAAGCAACTAAAAGAAACAGGCCACTTTCTTATA
TACACAGGAGGTTACCTAGAGCAAAATGGTACTTATTTAATTGGTAGCCCAGAGCTATTA
GGCTGTAAAGATGATGATATTTTAAGAATCATTAAGAATTCAGACATCGGTTGTGATTTA
GTGCCTAAGCTAGAAATTAATAACAGTCTGACAATTTATAATGCTTTGGATGATAATTGG
AACTCCAACTCATCACTGGGTTCAGATATCTCGAATGATGGTACCCCATTTTATTATATC
AAAAACGATTTGACCACCCAGCCTAGATCTTATAACGGTAATAGAGTCAATCGTGAACCG
GATTTTGAAAACAGCAGGAGCACGGAGGAAGAGTTTTATGAACTGGAGACAAGATTGAAC
TCTCTTGGTTATGTATTGGTATTGACTCCGCAAGAGCCATTACTTTGGGAGGGTGAGATG
TATAATCTATCCGATAACAAAACAATTAAACCAGAGGGATTGAACTTGAAAGTAATTCCT
AATTCAATAGATTTGATGTGCCAAGGATCCAGTTATGCCTTAGAATACCAATGTGACAGG
TTCCAACAGATATCTGGTAGCTCAGTTTCTTTCTTGGAAAAAAAATCTTCCTCAGAAACG
GTTAAAAACAACTTACAAAGGATAAATAAAGCATATTTCAGATGCACGTACAGTGTTCTG
AAGAACTATACAAAGATTGTGACCACGTTCAAGAAGGTAAGTCCTGTCAATGATTTATTG
AATAATATTTTCCTTTTTGGGAGGGATTTTGGTCTGAACTTTTTGAGAATTAATGTTGCC
AACAATGAAAAAAGATCCATTATAATACTTTTAATGATGCGGTTAAGTATCGGATGGCTG
AAGTTCTTAGCTGAAGACTGTGATCCGACTGATCAAAGGGTATTTAGGTGGTGTGTCACG
TCAATGGAGTTCGCGATGCATATGGTAAGCGGTTGGAACATACTAGCTCTTGATGAATGC
CAATTTTCTTCTTTAAAACAAAAAATTTCAGAATGCATGTCATTACTTATTTCTCATTTT
GATATAATAGGCGCACGCTCCATAGAAGTTGAGAAAATCAATCAACAAGCTAGATCAAAT
CTTGATTTGGAAGATGTGTTTGACGATGATATGATGCTACAAGTGAATTCCGAGTTCCGA
GTACAAAGTATAATGGAATTGGAAGAAAGGATAAAGCGGAATCCTCATCAAACTGGTAAA
GTAATTGATGATAGTGACAAAGGTAACAAGTACCTTGTTTCTTTGGCATCTTCCATATCG
AACGTTTCTATGAGATGGCAAAAGAGGAACTTTATTGGTGGCGGTACTTTTGGAAGGGTA
TATTCTGCTGTTGATTTGGATAATGGTGAGATTTTAGCAGTCAAGGAAATCAATATTCAA
GATAGCAAATCAATGCAAAAAATATTCCCCTTAATCAAGGAGGAAATGAGTGTCTTAGAG
ATATTGAACCATCCAAATATAGTTTCATATTACGGTGTTGAAGTTCATCGTGATAAAGTT
AACATCTTTATGGAATATTGTGAAGGCGGTTCCCTAGCAGCTCTTTTGGAGCATGGTCGT
ATTGAAGATGAAATGGTCACTCAAGTCTACACTTTACAATTGCTAGAAGGACTTGCATAT
TTGCATGAATCCGGCATTGTTCACCGTGATGTTAAACCCGAAAACATCCTACTAGATTTT
AATGGTGTTATTAAGTATGTTGATTTTGGTGCTGCTAAAAAAATTGCTAATAATGGAACT
AGATTGGCAAGTATGAACAAAATCGAAAACGCAGATGGTGAACACGAAGATGTTACCCAT
GTTTCTGATTCAAAGGCAGTGAAAAATAACGAAAATGCTCTATTAGACATGATGGGAACT
CCTATGTACATGGCTCCAGAATCCATCACTGGATCTACCACCAAAGGCAAACTTGGGGCA
GACGATGTTTGGTCGTTAGGCTGCGTGGTATTAGAAATGATCACTGGTAGACGGCCATGG
GCTAACTTAGATAATGAATGGGCTATCATGTACCATGTTGCTGCAGGACATACCCCACAA
TTCCCTACTAAGGATGAAGTGTCGTCTGCTGGTATGAAATTTCTGGAAAGATGTCTTATT
CAAAACCCCTCCAAGAGAGCCAGTGCGGTTGAGCTGTTGATGGATCCTTGGATTGTACAA
ATTAGAGAAATAGCCTTTGGCGACGATTCTTCCTCTACAGATACTGAGGAAAGAGAGTAG
Protein sequence predicted based on nucleotide sequence
MSHSDYFNYKPYGDSTEKPSSSKMRQSSSSSSSRLRSESLGRNSNTTQARVASSPISPGL HSTQYFRSPNAVYSPGESPLNTVQLFNRLPGIPQGQFFHQNAISGSSSSSARSSRRPSNI GLPLPKNPQQSLPKLSTQPVPVHKKVEASKTESEIIKKPAPVNSNQDPLLTTPTLVISPE LASLNTTNTSIMSTPQNITNQTSNKHIPTRSQPNGSTSSSTLQDIVTTNSSQRSVGHHGG STTSLRTYKKQYVLNEQLYLRKMRNRANDDYYTRGIVASSNFEDDEENFSNKGEDDLELE MDDLLKVEGEDKDNDFNFGYNFITSSTKNNENVVSMSLNYLKGKLDWLRDVNNDQPCEIE DEEWHSILGSEDLLSKLLQNPMVNNRFEWQTMLSKVLKGDIVRNEKTKIANQGKGPGFNT QFSDDIWIELKAWMNGRTVEDQNKSLRIFRDSTDSVFQEIMAFKLEDNMSADEAAETIKS LVDKYYRVLNLWPNIKRMHAEKPITKTEAFRNRIDTLNSWLNFKFNFDTNIAYLKKWIVG NKELESTTEVDNTTVNLDDPAVFATNCKRFAEQIMKEKDIELIFQKKIFFPLAPWILKAK FFFLKYQKTWNELNLSYLDQDLEFLLMFPMRLVKDIILIRLSYAKKIQNPTLMMIDQMMD DFSTYIKLAVQMKFTVASYCNDWFFKVKIDPEFDHTVVEGLEYFFSILELRILYSGKNSF KTSKEPDLLLKYWEMFRNVGYYIDDAGELIAAEFTKLTLRLVHRLHAYLLRQQNTPPKLE NEAAAEKWLVQIFEILGSMKRKLNRFTNILTKAFQNFVRYKIEDHNYLLKQLKETGHFLI YTGGYLEQNGTYLIGSPELLGCKDDDILRIIKNSDIGCDLVPKLEINNSLTIYNALDDNW NSNSSLGSDISNDGTPFYYIKNDLTTQPRSYNGNRVNREPDFENSRSTEEEFYELETRLN SLGYVLVLTPQEPLLWEGEMYNLSDNKTIKPEGLNLKVIPNSIDLMCQGSSYALEYQCDR FQQISGSSVSFLEKKSSSETVKNNLQRINKAYFRCTYSVLKNYTKIVTTFKKVSPVNDLL NNIFLFGRDFGLNFLRINVANNEKRSIIILLMMRLSIGWLKFLAEDCDPTDQRVFRWCVT SMEFAMHMVSGWNILALDECQFSSLKQKISECMSLLISHFDIIGARSIEVEKINQQARSN LDLEDVFDDDMMLQVNSEFRVQSIMELEERIKRNPHQTGKVIDDSDKGNKYLVSLASSIS NVSMRWQKRNFIGGGTFGRVYSAVDLDNGEILAVKEINIQDSKSMQKIFPLIKEEMSVLE ILNHPNIVSYYGVEVHRDKVNIFMEYCEGGSLAALLEHGRIEDEMVTQVYTLQLLEGLAY LHESGIVHRDVKPENILLDFNGVIKYVDFGAAKKIANNGTRLASMNKIENADGEHEDVTH VSDSKAVKNNENALLDMMGTPMYMAPESITGSTTKGKLGADDVWSLGCVVLEMITGRRPW ANLDNEWAIMYHVAAGHTPQFPTKDEVSSAGMKFLERCLIQNPSKRASAVELLMDPWIVQ IREIAFGDDSSSTDTEERE*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
4740
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
