Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
FLH203068.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pDONR221
Format:
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
HIP
ScCD00025176
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 9432nts
Open reading frame: 1 to 9432
ATGTTAGAGTCTTTAGCTGCTAATTTGCTAAACCGATTGCTAGGCTCATATGTGGAAAAT
TTCGACCCTAATCAATTAAATGTCGGCATATGGAGTGGTGATGTGAAGTTAAAAAACTTA
AAACTAAGAAAAGACTGTTTGGACTCATTAAATTTACCCATAGATGTGAAATCAGGGATA
CTGGGTGATTTGGTCTTAACGGTCCCATGGTCAAGTTTAAAGAATAAGCCCGTGAAAATC
ATTATCGAGGACTGTTATTTATTATGCTCACCACGTTCAGAAGATCACGAAAATGATGAG
GAGATGATAAAGAGAGCTTTTCGGTTGAAGATGAGGAAGGTAAGTGAATGGGAACTGACA
AATCAGGCTCGCATACTGAGTACCCAATCAGAGAACAAAACCTCTTCTTCTTCTTCAGAA
AAAAATAACGCCGGGTTTATGCAGTCTTTGACTACAAAGATCATAGACAATTTACAAGTT
ACCATAAAAAATATCCATTTAAGGTACGAGGACATGGACGGAATCTTCACTACAGGCCCA
TCTTCCGTTGGATTAACTTTGAACGAGCTATCAGCTGTATCTACAGATTCCAACTGGGCT
CCAAGTTTCATTGATATTACACAGAATATCACGCATAAACTATTGACTTTAAATTCACTC
TGTTTGTATTGGAATACAGACTCTCCGCCCTTGATTTCTGATGATGATCAAGATAGAAGC
CTGGAAAATTTTGTAAGGGGTTTCAAAGATATGATTGCATCGAAAAATTCCACTGCCCCC
AAGCATCAATACATTTTGAAACCGGTTAGCGGGCTAGGGAAGCTTAGTATCAACAAGCTG
GGTTCCACGGAGGAACAACCTCACATTGACTTGCAAATGTTTTATGATGAATTTGGTTTA
GAATTAGATGATACAGAATATAATGATATCTTGCATGTCCTCTCCAGTATACAGTTAAGA
CAAATTACCAAAAAGTTTAAGAAAGCAAGACCATCCTTCGCAGTTAGTGAAAACCCGACA
GAATGGTTTAAATACATTGCTGCTTGTGTTATTAATGAAATTCATGAAAAAAACAAAATG
TGGACTTGGGAAAGCATGAAGGAGAAATGTGAACAACGGAGGTTGTACACAAAATTATGG
GTTGAAAAGTTAAAGTTAAAAAATTTAGAAGCTCCGCTACGTGACCCCATTCAGGAGGCT
CAATTATCTGAATTGCATAAAGATTTAACGTATGATGAAATTATTCTCTTCAGATCTGTC
GCTAAGAGACAGTACGCGCAATATAAGTTGGGCATGACGGAAGACTCACCCACACCAACA
GCTAGCTCAAATATTGAACCACAAACTTCCAACAAGTCCGCAACTAAAAATAATGGAAGC
TGGCTCTCTTCTTGGTGGAACGGTAAACCTACAGAAGAAGTAGACGAAGATTTAATAATG
ACTGAAGAACAGCGTCAGGAGCTTTATGATGCTATTGAATTTGACGAGAATGAAGATAAA
GGTCCTGTACTGCAAGTTCCTAGAGAAAGGGTTGAACTTCGTGTAACGAGTTTGTTAAAA
AAGGGTTCATTCACTATACGAAAGAAGAAGCAAAATCTAAATTTGGGTTCCATAATTTTT
GAAAATTGTAAAGTTGATTTTGCTCAAAGACCTGATTCATTTTTATCGAGTTTCCAATTG
AACAAATTCAGTCTCGAAGATGGCTCACCCAACGCGTTATATAAACATATCATTAGTGTC
AGAAACTCATCTAAAGATCAATCTTCAATTGACAACCACGCTACCGGGGAGGAAGAAGAG
GATGAACCTTTATTACGTGCTTCATTTGAATTGAATCCACTAGATGGATTAGCAGATTCG
AACTTAAACATTAAGTTATTGGGAATGACTGTGTTTTACCACGTTCATTTCATTACAGAG
GTCCACAAGTTTTTTAAGGCGTCAAACCAACACATGGAAACAATCGGCAATATCGTAAAC
GCCGCCGAGGCAACTGTAGAAGGTTGGACAACACAAACAAGAATGGGTATTGAGTCTTTG
TTGGAAGACCACAAGACCGTAAATGTATCTTTAGATTTACAAGCACCATTAATCATATTA
CCGTTAGACCCACATGATTGGGATACCCCTTGTGCGATCATTGATGCCGGTCATATGTCT
ATACTCAGTGATTTAGTGCCAAAGGAGAAGATAAAGGAAATAAAAGAGTTATCGCCGGAA
GAATATGACAAGATTGACGGTAACGAAATCAATAGGTTAATGTTTGATAGGTTCCAAATT
TTATCTCAGGATACTCAGATATTTGTTGGTCCTGATATTCAGTCTACTATAGGTAAGATC
AATACCGCCTCTTCCACGAATGATTTTAGAATCTTGGATAAAATGAAATTAGAGTTAACT
GTAGATCTATCAATCCTTCCTAAAGCATATAAATTACCCACGATAAGAGTTTTTGGTCAT
TTGCCTCGCTTAAGTCTTTCCATAAATGACATTCAATACAAGACTATTATGAATTTGATT
GCTAATTCTATACCTTCTATGATTGATGATGAAGAAAACAACGGCGATTACGTTAATTAC
TCTTCTGGTTCCGAAAAAGAAATGAAGAAGCAAATTCAATTGCAGTTGAAGAATACTTTG
AAAGCGTTAGAAAATATGCAGCCGCTTCAAATTGAACAGAAATTCTTAGAGCTCCATTTT
GATATCGATCAGGCGAAAATAGCATTCTTTCAATGTATCAAAAATGATTCCAGAAACAGC
GAAAAATTGGTTGATATATTGTGTCAAAGGCTAAATTTCAATTTTGATAAGAGAGCCAAA
GAAATGAATTTAGATCTAAGAGTACATTCATTAGATGTTGAAGATTACATCGAGCTGACT
GATAACAAGGAATTCAAAAACTTGATATCATCCGGCGTTGAAAAAGTGACAAGATCTCAA
AAAGATTTGTTTACGCTAAAATACAAGAGAGTGCAGAGAATTGTGCCTCATAACGATACT
TTGATTGAATTATTCGATCAAGATATTGTTATGCATATGAGTGAATTGCAATTGGTATTA
ACACCTAGATCTGTATTAACTTTAATGAATTATGCAATGCTTACTTTTACGGATCCTAAC
GCTCCAGAAATGCCGGCTGATGTGTTGAGACATAATAAAGAAGATAGGGACGATGCGCCA
CAAAAAATCAATATGAAAATTAAAATGGAGGCCGTCAATGTTATTTTTAATGATGATTCT
ATAAAGCTGGCAACGTTAGTTCTATCGGCTGGTGAATTCACAATGGTTTTACTACCGGAA
AGATACAACATTAACTTAAAATTAGGCGGGTTGGAGTTGACTGATGAAACAAATGAATCG
TTTTCCAGGGATTCGGTATTTAGAAAAATAATTCAAATGAAAGGTCAAGAACTAGTCGAA
TTGAGCTATGAATCATTCGATCCGGCTACAAATACCAAAGATTATGATTCGTTTTTAAAA
TATTCTACGGGCTCTATGCATGTTAATTTTATCGAAAGTGCTGTTAACAGAATGGTTAAC
TTTTTTGCTAAATTTCAAAAATCTAAAGTATCCTTTGATAGAGCTCGTCTTGCAGCGTAC
AACCAAGCCCCTTCCATTGACGCCGTAAATAACATGAAAATGGACATAGTAATCAAGGCA
CCTATTATCCAGTTTCCTAAATTAGTAGGCACACAAGAGAACAATTACGATACAATGCGG
TTTTATTTGGGTGAGTTCTTTATTGAAAACAAATTTTCGGTAATAGACGAATCTCATAAA
ATAAACCATATCAAACTTGGTGTCCGCGAGGGCCAATTATCCTCGAACTTAAACTTCGAT
GGTTCCAGTCAACAATTGTATCTAGTTGAAAATATAGGTTTACTTTTCAATATAGACAGA
GATCCCCTTCCGCAAGATGACACTCCAGAACTTAAAGTAACGAGTAATTTTGAATCATTT
GCGTTGGATTTGACTGAGAACCAGCTGACTTACTTATTAGAAATTTCCAATAAAGTATCC
TCTGCATTCAATATCACAGACGAAAACTCTGGTGAATCAGGAGGAAAAGGCGAGATCAAA
AGTCCCTCTCCTGACCCTGCTTCTTTAAGTAGCGAAAGCGAACGCACAGCTACTCCTCAG
TCTCTACAGGGATCGAATAAAAGCAATATAAAAAATCCAGAACAAAAATACTTGGATTTT
TCTTTTAAAGCTCCAAAGATTGCCCTTACTTTATATAACAAGACAAAGGGCGTCACTTCT
TTGAATGACTGTGGTCTGACAAGAATTATGTTTCAAGACATTGGGTGCAGCCTTGGTTTA
AAGAATGATGGGACCGTCGATGGTCAAGCACATGTTGCTGCTTTCCGGATAGAAGATGTT
CGTAATATAAAAGATAATAAGCATACAGAACTAATACCAAAGAGTAAGAATAAAGAATAT
CAATTTGTGGCTAATATTTCCAGAAAGAACTTAGAGGTCGGACGGCTATTGAACATTTCG
ATGACAATGGACAGTCCGAAAATGATTTTGGCAATGGACTATTTGGTTTCTCTCAAAGAA
TTTTTTGATGCAATTATGTCCAAAAGCCACGAAAACAATTTATACTATCCTGAAAACACA
AACCAAAAGCCTGAAAACAAGGCTATTGTTGAAAGCGTTCAAGAAGGTGGTGATGTGACT
AAAATCCAATACTCTGTTAATATCATCGAAACGGCTCTGATCTTACTTGCTGATCCTTGC
GACATGAATAGTGAGGCTATTTCTTTCAAGATAGGACAATTTTTAGTAACCGATCAGAAT
ATTATGACAGTTGCAGCCAATAATGTTGGTATCTTCCTATTCAAAATGAATTCGTCTGAA
GAAAAATTGAGATTATTGGACGACTTTTCATCCTCCTTGACAATTGATAAAAGAAACTCA
ACTCCTCAGACCCTAATGACGAATATACAGCTTTCCGTTCAGCCCCTGTTGATGCGTATT
TCACTTAGAGATATTCGTCTGGCGATGTTAATATTCAAACGAGTTACCACATTGCTAAAT
AAGATGACGGAAAAAGAGGATAATGGAGAGGAGGAGGAGAGCACAGACAAAATACAATTT
TCTCATGAATTTGAAAGGAAATTAGCGGTGTTGGATCCAAGCATTTTAGGTGAGCGCAGC
AGGGCTAGCCAGAGCTCAGATTCCGAATCAATTGAAGTGCCTACTGCAATTTTGAAAAAC
GAAACATTCAATGCTGACTTAGGCGGACTGAGATTTATTTTAATTGGTGACGTCCATGAA
ATGCCAATATTGGACATGAATGTAAATGAAATCACTGCTTCAGCAAAGGATTGGTCTACC
GACTTTGAAGCTCTTGCTTCTCTAGAAACCTATGTTAACATATTCAACTATTCGAGATCT
AGCTGGGAGCCACTTCTGGAGATGATTCCAATAACATTCCACCTATCAAAAGGGCATTCT
GAAATGGATCCTGCTTTCTCTTTTGATATTTTAACTCAAAGAATCGCGGAAATTACACTT
TCTGCTAGGTCAATCGCTATGCTTTCTCACATCCCGGCGTCTCTAACCGAAGAGCTGCCC
TTAGCTTCCAGGGTATCTCAGAAGCCATATCAACTGGTAAACGATACAGAATTAGATTTT
GACGTCTGGATACAGGATAAAACGACTGAAGACAACAAAAACGAAGTTGTGCTCTTAAAA
GCAAACACTTCTCTTCCTTGGGAATTTGAAGATTGGAGAAGTATTAGAGAGAAATTGGAT
ATTGATAAGTCGAAGAACATTTTGGGTGTTTGTGTTTCTGGGCAAAATTATAAAACCATT
ATGAATATTGATGCCACAACAGAAGGTGAAAACTTGCATGTTTTGAGCCCACCAAGAAAT
AATGTGCATAATAGAATTGTTTGTGAGGCCAGATGTGATGAGAATAACGTGAAGATTATT
ACGTTTAGGTCAACTTTAGTTATCGAAAACACAACTTCAACTGAGATTGAGCTTTTGGTT
GACTCCAAAGATCCAAATAAGCCATCTTTAAAATATGCTATTAAACCACATCAAAGTAAA
TCTGTTCCTGTAGAATATGCCTACGATTCTGATATTAGGATAAGACCCGCATCAGAGGAT
ATATACGATTGGTCTCAACAGACGTTATCTTGGAAAAGCTTGTTGAGCAACCAAATGTCT
ATATTCTGTTCTTCAAAAGAAGATTCCAATCAAAGATTTCATTTCGAAATTGGTGCAAAG
TATGATGAAAGAGAACCATTGGCAAAGATCTTTCCTCATATGAAAATCGTGGTTTCGGCA
TCTATGACTATTGAAAATTTACTTCCAGCAGATATTAACTTCTCTATTTTTGACAAAAGA
GAGGAGAAGAGAACAGATTTCTTGAAGACTGGAGAAAGTATGGAAGTTCATCATATTTCT
TTGGATAGTTTCCTGCTAATGTCTGTTCAACCATTACAAGATGAGGCTTCTGCTTCAAAG
CCCTCCATCGTTAATACACCACATAAAAGTCCTTTAAACCCAGAAGATTCGTTGTCTTTG
ACCCTATCTGGAGGTCAGAATTTGTTGTTGAAACTAGATTATAAAAACATCGATGGTACA
AGGTCCAAAGTTATTCGGATATACTCCCCTTATATTATTATGAACTCTACCGATAGAGAA
CTCTACATTCAAAGTAGTCTCTTAAATATTGCACAATCCAAGATTTTGCTTGAAAACGAA
AAAAGATATACTATTCCAAAAATGTTTTCATTTGATAAAGAAGATGATAAAAGTAACAGA
GCTCGTATTAGGTTCAAGGAATCAGAGTGGAGTTCCAAATTATCATTTGACGCTATTGGC
CAATCATTTGATGCATCTGTTCGCATAAAAAATAAGGAACAAGAATCAAATTTAGGCATC
AACATTAGCGAAGGTAAAGGGAAGTACTTGCTAAGTAAAGTTATTGAGATTGCACCTAGG
TATATTATAAGCAATACTTTAGATATCCCTATTGAAGTTTGCGAGACAGGGTCTATGGAT
GTCCAGCAAATAGAATCGAACATCACTAAGCCTTTATACAGGATGAGAAACATCGTCGAC
AAGCAATTAGTGCTGAAATTCTTAGGCGGAGACTCCAATTGGTCACAACCATTCTTTATC
AAAAATGTCGGTGTAACATATTTGAAAGTTTTGAAAAATTCACGCCATAAGCTTTTAAAG
ATTGAAATTCTTTTGGACAAAGCAACAATATTCATAAGAATCAAGGACGGTGGTGACAGA
TGGCCATTTTCCATAAGAAATTTCAGCGACCATGACTTTATATTTTACCAAAGAGATCCT
CGTAAAGTTAGTGATCCATACAAGGATGACCAGTCTAACGAGTCCTCAAGCCGTAGTTTC
AAACCTATCTTTTACAGGATTCCCTCGAAAAGTATTATGCCATACGCATGGGATTTTCCT
ACAGCTAAGGAGAAATATCTGGTGTTAGAATCAGGTACACGTACAAGGGAAGTTCGGTTG
GCTGAGATTGGCGAATTGCCACCGTTACGCCTGGATAAAAGGTCAAAGGATAAACCAGCC
CCCATTGTTGGATTACATGTTGTTGCTGACGACGATATGCAGGCCTTAGTTATTGTTAAC
TATAAGGCCAATGTTGGGTTATACAAATTGAAAACTGCCTCTGCAACTACCACGTCTTCA
GTTTCAGTAAATAGTTCAGTGACTGATGGCTTTGTGCAAAAGGATGAGGATGAGAAGGTT
AATACACAGATAGTTGTTTCGTTCAAAGGAGTTGGTATCTCATTAATCAATGGTCGTTTA
CAGGAACTTCTATATATTAACATGAGAGGGATCGAATTAAGATACAATGAATCTAAAGCA
TACCAGACTTTCAGTTGGAAAATGAAATGGATGCAAATAGATAATCAATTATTTTCAGGA
AACTACTCAAATATTCTATATCCAACAGAAATCCCCTACACAGAAAAGGAAATTGAAAAT
CATCCAGTGATATCAGGTTCTATTTCTAAAGTCAACGATTCCTTACAGGCTGTCCCATAC
TTTAAACATGTTACGTTATTAATCCAGGAATTTTCCATACAATTAGATGAAGACATGCTG
TATGCTATGATGGACTTCATAAAATTCCCAGGATCCCCATGGATCATGGATTCAAGAGAT
TATAAGTACGATGAAGAAATCCAGTTACCTGATGTTTCTGAATTAAAAACGGCGGGTGAT
ATATATTTCGAGATCTTCCATATTCAGCCCACCGTCTTACATCTGTCCTTTATACGCTCC
GATGAAATTAGTCCAGGATTGGCTGAAGAGACCGAGGAGTCATTTTCTTCATCATTGTAC
TATGTCCATATGTTTGCTATGACATTAGGTAATATAAATGAGGCGCCGGTTAAAGTTAAT
TCTTTGTTCATGGATAATGTAAGAGTCCCATTGCCCATTCTGATGGACCATATTGAGAGA
CACTATACGACCCAGTTCGTGTATCAGATCCACAAAATTTTAGGATCAGCAGATTGTTTT
GGTAATCCTGTAGGTCTTTTCAACACAATTAGTTCCGGTGTCTGGGACTTATTCTATGAA
CCATATCAAGGTTACATGATGAATGATAGGCCTCAAGAAATTGGTATACATCTGGCTAAA
GGTGGACTAAGTTTTGCAAAGAAAACAGTCTTTGGCCTCTCTGATAGTATGTCCAAGTTT
ACGGGGTCGATGGCCAAGGGCCTGTCAGTTACACAAGATCTGGAATTCCAAAGGGTCAGG
AGATTACAACAAAGAATAAATAAAAACAACAGGAATGCCTTGGCAAACTCAGCGCAATCC
TTTGCCAGCACACTGGGAAGCGGGTTGAGCGGAATTGCACTGGACCCATACAAGGCCATG
CAAAAGGAAGGCGCCGCTGGTTTCCTCAAAGGCCTTGGTAAAGGTATCGTTGGGCTGCCA
ACTAAGACAGCCATTGGATTCTTAGATCTTACAAGTAACTTAAGTCAAGGCGTGAAAAGT
ACCACCACTGTGCTTGACATGCAAAAGGGTTGCAGAGTGCGTCTACCAAGGTACGTGGAC
CATGATCAAATCATCAAGCCTTATGATCTACGTGAGGCCCAAGGACAATACTGGCTCAAG
ACCGTGAATGGAGGAGTATTTATGAATGATGAATACCTGTCGCACGTTATATTGCCAGGC
AAAGAACTAGCAGTAATTGTGTCCATGCAGCACATTGCTGAGGTGCAAATGGCCACCCAA
GAGCTTATGTGGAGCACAGGATATCCTTCCATCCAGGGAATTACATTGGAAAGATCCGGT
CTTCAAATTAAACTCAAGTCTCAATCCGAATACTTCATTCCCATCAGCGATCCTGAAGAA
CGTCGGTCCTTGTACAGGAACATCGCCATTGCTGTTAGAGAATACAATAAGTACTGTGAA
GCTATCCTATAG
Protein sequence predicted based on nucleotide sequence
MLESLAANLLNRLLGSYVENFDPNQLNVGIWSGDVKLKNLKLRKDCLDSLNLPIDVKSGI LGDLVLTVPWSSLKNKPVKIIIEDCYLLCSPRSEDHENDEEMIKRAFRLKMRKVSEWELT NQARILSTQSENKTSSSSSEKNNAGFMQSLTTKIIDNLQVTIKNIHLRYEDMDGIFTTGP SSVGLTLNELSAVSTDSNWAPSFIDITQNITHKLLTLNSLCLYWNTDSPPLISDDDQDRS LENFVRGFKDMIASKNSTAPKHQYILKPVSGLGKLSINKLGSTEEQPHIDLQMFYDEFGL ELDDTEYNDILHVLSSIQLRQITKKFKKARPSFAVSENPTEWFKYIAACVINEIHEKNKM WTWESMKEKCEQRRLYTKLWVEKLKLKNLEAPLRDPIQEAQLSELHKDLTYDEIILFRSV AKRQYAQYKLGMTEDSPTPTASSNIEPQTSNKSATKNNGSWLSSWWNGKPTEEVDEDLIM TEEQRQELYDAIEFDENEDKGPVLQVPRERVELRVTSLLKKGSFTIRKKKQNLNLGSIIF ENCKVDFAQRPDSFLSSFQLNKFSLEDGSPNALYKHIISVRNSSKDQSSIDNHATGEEEE DEPLLRASFELNPLDGLADSNLNIKLLGMTVFYHVHFITEVHKFFKASNQHMETIGNIVN AAEATVEGWTTQTRMGIESLLEDHKTVNVSLDLQAPLIILPLDPHDWDTPCAIIDAGHMS ILSDLVPKEKIKEIKELSPEEYDKIDGNEINRLMFDRFQILSQDTQIFVGPDIQSTIGKI NTASSTNDFRILDKMKLELTVDLSILPKAYKLPTIRVFGHLPRLSLSINDIQYKTIMNLI ANSIPSMIDDEENNGDYVNYSSGSEKEMKKQIQLQLKNTLKALENMQPLQIEQKFLELHF DIDQAKIAFFQCIKNDSRNSEKLVDILCQRLNFNFDKRAKEMNLDLRVHSLDVEDYIELT DNKEFKNLISSGVEKVTRSQKDLFTLKYKRVQRIVPHNDTLIELFDQDIVMHMSELQLVL TPRSVLTLMNYAMLTFTDPNAPEMPADVLRHNKEDRDDAPQKINMKIKMEAVNVIFNDDS IKLATLVLSAGEFTMVLLPERYNINLKLGGLELTDETNESFSRDSVFRKIIQMKGQELVE LSYESFDPATNTKDYDSFLKYSTGSMHVNFIESAVNRMVNFFAKFQKSKVSFDRARLAAY NQAPSIDAVNNMKMDIVIKAPIIQFPKLVGTQENNYDTMRFYLGEFFIENKFSVIDESHK INHIKLGVREGQLSSNLNFDGSSQQLYLVENIGLLFNIDRDPLPQDDTPELKVTSNFESF ALDLTENQLTYLLEISNKVSSAFNITDENSGESGGKGEIKSPSPDPASLSSESERTATPQ SLQGSNKSNIKNPEQKYLDFSFKAPKIALTLYNKTKGVTSLNDCGLTRIMFQDIGCSLGL KNDGTVDGQAHVAAFRIEDVRNIKDNKHTELIPKSKNKEYQFVANISRKNLEVGRLLNIS MTMDSPKMILAMDYLVSLKEFFDAIMSKSHENNLYYPENTNQKPENKAIVESVQEGGDVT KIQYSVNIIETALILLADPCDMNSEAISFKIGQFLVTDQNIMTVAANNVGIFLFKMNSSE EKLRLLDDFSSSLTIDKRNSTPQTLMTNIQLSVQPLLMRISLRDIRLAMLIFKRVTTLLN KMTEKEDNGEEEESTDKIQFSHEFERKLAVLDPSILGERSRASQSSDSESIEVPTAILKN ETFNADLGGLRFILIGDVHEMPILDMNVNEITASAKDWSTDFEALASLETYVNIFNYSRS SWEPLLEMIPITFHLSKGHSEMDPAFSFDILTQRIAEITLSARSIAMLSHIPASLTEELP LASRVSQKPYQLVNDTELDFDVWIQDKTTEDNKNEVVLLKANTSLPWEFEDWRSIREKLD IDKSKNILGVCVSGQNYKTIMNIDATTEGENLHVLSPPRNNVHNRIVCEARCDENNVKII TFRSTLVIENTTSTEIELLVDSKDPNKPSLKYAIKPHQSKSVPVEYAYDSDIRIRPASED IYDWSQQTLSWKSLLSNQMSIFCSSKEDSNQRFHFEIGAKYDEREPLAKIFPHMKIVVSA SMTIENLLPADINFSIFDKREEKRTDFLKTGESMEVHHISLDSFLLMSVQPLQDEASASK PSIVNTPHKSPLNPEDSLSLTLSGGQNLLLKLDYKNIDGTRSKVIRIYSPYIIMNSTDRE LYIQSSLLNIAQSKILLENEKRYTIPKMFSFDKEDDKSNRARIRFKESEWSSKLSFDAIG QSFDASVRIKNKEQESNLGINISEGKGKYLLSKVIEIAPRYIISNTLDIPIEVCETGSMD VQQIESNITKPLYRMRNIVDKQLVLKFLGGDSNWSQPFFIKNVGVTYLKVLKNSRHKLLK IEILLDKATIFIRIKDGGDRWPFSIRNFSDHDFIFYQRDPRKVSDPYKDDQSNESSSRSF KPIFYRIPSKSIMPYAWDFPTAKEKYLVLESGTRTREVRLAEIGELPPLRLDKRSKDKPA PIVGLHVVADDDMQALVIVNYKANVGLYKLKTASATTTSSVSVNSSVTDGFVQKDEDEKV NTQIVVSFKGVGISLINGRLQELLYINMRGIELRYNESKAYQTFSWKMKWMQIDNQLFSG NYSNILYPTEIPYTEKEIENHPVISGSISKVNDSLQAVPYFKHVTLLIQEFSIQLDEDML YAMMDFIKFPGSPWIMDSRDYKYDEEIQLPDVSELKTAGDIYFEIFHIQPTVLHLSFIRS DEISPGLAEETEESFSSSLYYVHMFAMTLGNINEAPVKVNSLFMDNVRVPLPILMDHIER HYTTQFVYQIHKILGSADCFGNPVGLFNTISSGVWDLFYEPYQGYMMNDRPQEIGIHLAK GGLSFAKKTVFGLSDSMSKFTGSMAKGLSVTQDLEFQRVRRLQQRINKNNRNALANSAQS FASTLGSGLSGIALDPYKAMQKEGAAGFLKGLGKGIVGLPTKTAIGFLDLTSNLSQGVKS TTTVLDMQKGCRVRLPRYVDHDQIIKPYDLREAQGQYWLKTVNGGVFMNDEYLSHVILPG KELAVIVSMQHIAEVQMATQELMWSTGYPSIQGITLERSGLQIKLKSQSEYFIPISDPEE RRSLYRNIAIAVREYNKYCEAIL*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
9435
Mutations:
None
Discrepancy:
del@1796,3; g8331t
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
