Gene:
Gene Symbol:
TM0538
Gene Name:
cation efflux system protein
Original Clone ID:
24466
Keyword:
None
Species:
Thermotoga maritima
Type:
cDNA
Vector Name:
pMH2T7TEV
Format:
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
Joint Center for Structural Genomics
Joint Center for Structural Genomics
TmCD00281994
Price:
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 855nts
Open reading frame: 51 to 905
CTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCATGAAGAAGA
AACTGCTGTTCTCCATATGGCTGAACGCCATCATAACACTCTCTGAAGTGGTCGGTGGCT
TGATATCCGGGAGTCTCGCCCTTCTGGGCGACTCCCTTCATAATTTTTCCGATACCATGA
GTCTTCTTGGAAGTTTCATCGCGATGAAGATCAGCGAGAAACCAAAGAACAAAAAGTACA
CCTTCGGCTACAGAAGAAGCGAAATCATTGTGGCTTTCTTGAACTCCGTATCCATCTTCG
TTGTTTCAACACTCGTTGTCATCGAAGCGGTGAAAAGATTGTTGAGTCCCGCCACCGTTC
ACACGTCGGTGCTTCTCCTCGTCTCCTCGATAGGTCTCGCTGCCAACTTCTTTTCTGTCA
TCCTTCTTCACACCCACAGCAAAGAAAGTATGAACGTTCGATCTGCGTACCTGCATCTCA
TCGCGGATACACTGTCCTCGATCCTTGTGGTTCTTGGGGCTGTTTTCATGAGGGTCTGGA
AAATATACTGGCTCGACCCTGTTCTCGCTTTCGTTATAGCCTTATACATGTTCAAAGAAG
CCTATGAGATAGTGAAGGAGTCTCTTGAAATTCTCATGGAAGCCTCACCGAACCTGGACT
TTGAGAAAATAAAGGAGGAAATTGAAAAAATAGAAGGTGTGAGGAACGCCCACCATTTTC
ACGCCTGGAGAGTGGGAGAGAAAGAAATCCACTTCGAGTGTCACGTTGAAGTGGACAACA
TGGAGCTGAAGGACGCCCAGAAGATCATAGACGAAATAGAAAAAAGATTGAAAAAATACG
GGATAACCCACACCACCGTTCAGCTCGAATGTGAAAGGTGCAGCAAAGAGATGATCTGCA
GTTAAGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTT
Protein sequence predicted based on nucleotide sequence
MKKKLLFSIWLNAIITLSEVVGGLISGSLALLGDSLHNFSDTMSLLGSFIAMKISEKPKN KKYTFGYRRSEIIVAFLNSVSIFVVSTLVVIEAVKRLLSPATVHTSVLLLVSSIGLAANF FSVILLHTHSKESMNVRSAYLHLIADTLSSILVVLGAVFMRVWKIYWLDPVLAFVIALYM FKEAYEIVKESLEILMEASPNLDFEKIKEEIEKIEGVRNAHHFHAWRVGEKEIHFECHVE VDNMELKDAQKIIDEIEKRLKKYGITHTTVQLECERCSKEMICS*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
855
Mutations:
None
Discrepancy:
ins@1,1,ins@1,1,ins@1,1,ins@1,1,ins@1,1,ins@1,1,ins@1,1,ins@1,1,ins@1,1,ins@1,1,ins@1,1,ins@1,1,ins@1,1,ins@1,1,ins@1,1,ins@1,1,ins@1,1,ins@1,1,ins@1,1,ins@1,1,ins@1,1;
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Protein_Purified
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in 282411
Recommended expression in:
Not Applicable
Vector Name:
pMH2T7TEV
Synonyms:
pMH-2-T7-TEV
Sequencing Primer:
Forward:
pBAD forward
Reverse:
alt_bad_rev
Description:
Bacterial expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHHENLYFQG tag; amp resistance; PIPE cloning.
Comments:
None
Size (bp):
4105
Parent Vector:
None
Properties:
PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker
Author Name:
Joint Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
ColEI origin of replication
1942
2624
inducible trxn activator protein gene
araC
araC gene
3199
4074
promoter
T7
T7 promoter
290
310
protease cleavage site
TEV
TEV protease cleavage site
382
402
selectable marker
AmpR
ampicillin resistance gene
1185
1844
tag
His
N-terminal 6xHis tag
364
381
trxn regulatory element
arabinose O1 operator
arabinose O1 operator
161
182
trxn regulatory element
arabinose O2 operator
arabinose O2 operator
5
23
trxn termination sequence
rrnB
rrnB T2 transcription termination
849
875
