DNASU Plasmid | TM0276 (T. maritima ) in pMH2T7 (bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: TM0276

Gene Name: L-arabinose isomerase

Original Clone ID:

8070

Keyword:

None

Species:

Thermotoga maritima

Type:

cDNA

Vector Name:

pMH2T7

Format:

CLOSED

Source:

Joint Center for Structural Genomics

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

PSI
Joint Center for Structural Genomics

TmCD00282195

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 1491nts

Open reading frame: 37 to 1527

ATGGGATCTGATAAAATTCATCATCATCATCATCACATGATAGATCTCAAGCAGTACGAG
TTCTGGTTTCTCGTTGGAAGTCAGTATCTTTACGGTCTGGAAACCCTGAAAAAGGTGGAG
CAACAGGCAAGTAAGATCGTGGATTCACTGAACGATGACCCCATCTTCCCTTCAAAGATC
GTTTTAAAGCCGGTTTTGAAGAGTTCTTCTGAAATCACGGAAATCTTTGAGAAGGCAAAC
GCAGACCCCAAATGCGCAGGGGTTATCGTCTGGATGCACACTTTTTCCCCATCGAAGATG
TGGATACGGGGACTCTCCATAAACAAAAAACCCCTGCTTCACCTTCACACGCAGTACAAC
AGAGAGATTCCATGGGATACGATCGATATGGATTACATGAACCTGAACCAGTCCGCACAC
GGAGACAGAGAGCATGGGTTCATACATGCAAGGATGAGACTTCCAAGGAAAGTTGTGGTG
GGACACTGGGAAGAGAAAGAAGTCAGAGAAAAGATCGCAAAGTGGATGAGAGTGGCCTGT
GCGATACAGGATGGAAGAATGGGACAGATAGTCAGGTTCGGTGACAACATGAGAGAAGTC
GCCAGCACCGAAGGCGACAAAGTAGAAGCACAGATAAAACTCGGCTGGTCCATAAACACT
TGGGGAGTTGGAGAACTTGCAGAGAGAGTGAAAGCCGTTCCAGAGCGCGAAGTAGAGGAA
CTTCTCAAAGAATACAGAGAAAAATACATCATGCCAGAGGATGAATACAGTCTCAAAGCA
ATAAGAGAGCAGGCGAAAATAGAAATTGCTCTGAGAGAATTTTTGAAAGAAAAGAACGCT
GTTGGTTTCACCACCACGTTCGAGGACCTGCACGATCTTCCACAGCTTCCCGGGCTCGCG
GTCCAGAGACTCATGGAGGAAGGATACGGTTTTGGTGCAGAAGGTGACTGGAAAGCAGCG
GGATTGGTTAGAGCTATCAAGGTAATGGGAACGAGTCTTCCCGGCGGAACTTCTTTTATG
GAAGACTACACTTACCACCTCACTCCCGGAAATGAACTCGTTTTAGGGGCTCACATGCTC
GAGGTATGTCCAACGATAGCAAAGGAAAAACCACGAATAGAGGTTCACCCTCTCAGCATC
GGCGGGAAAGCAGATCCTGCCCGTCTTGTCTTCGATGGTCAGGAAGGACCAGCCGTTAAC
GCATCGATCGTTGATATGGGCAACAGGTTCAGACTCGTTGTGAACAAGGTTTTATCCGTT
CCCATAGAGAGGAAGATGCCAAAACTCCCGACAGCCAGGGTCCTCTGGAAACCGCTGCCT
GATTTCAAGAGGGCAACTACTGCATGGATACTCGCTGGAGGATCACACCACACTGCCTTC
TCAACAGCCATTGACGTAGAATACCTCATCGACTGGGCGGAGGCATTGGAAATCGAATAC
GTCGTCATCGATGAGAATTTGGATCTCGAGGACTTCAAAAAAGAACTGAGATGGAACGAA
CTCTACTGGGGGCTTTTGAAAAGATAATTAA

Protein sequence predicted based on nucleotide sequence

MIDLKQYEFWFLVGSQYLYGLETLKKVEQQASKIVDSLNDDPIFPSKIVLKPVLKSSSEI
TEIFEKANADPKCAGVIVWMHTFSPSKMWIRGLSINKKPLLHLHTQYNREIPWDTIDMDY
MNLNQSAHGDREHGFIHARMRLPRKVVVGHWEEKEVREKIAKWMRVACAIQDGRMGQIVR
FGDNMREVASTEGDKVEAQIKLGWSINTWGVGELAERVKAVPEREVEELLKEYREKYIMP
EDEYSLKAIREQAKIEIALREFLKEKNAVGFTTTFEDLHDLPQLPGLAVQRLMEEGYGFG
AEGDWKAAGLVRAIKVMGTSLPGGTSFMEDYTYHLTPGNELVLGAHMLEVCPTIAKEKPR
IEVHPLSIGGKADPARLVFDGQEGPAVNASIVDMGNRFRLVVNKVLSVPIERKMPKLPTA
RVLWKPLPDFKRATTAWILAGGSHHTAFSTAIDVEYLIDWAEALEIEYVVIDENLDLEDF
KKELRWNELYWGLLKR*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

Start on reference sequence 1

End on reference sequence 1491

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinExpressed: Protein_Confirmed

ProteinPurified: Protein_Purified

SolubleProtein: Protein_Soluble

Find experimental protocols in 282153

Recommended expression in:

Not Applicable

Vector Name:

pMH2T7

Synonyms:

pMH-2-T7

Sequencing Primer:

Forward: pBAD forward

Reverse: alt_bad_rev

Description:

Bacterial (E. coli) expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHH tag; amp resistance; restriction enzyme (Pml I/Pac I) or PIPE cloning.

Comments:

None

Size (bp):

4084

Parent Vector:

None

Properties:

PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker

Author Name:

Joint Center for Structural Genomics

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

AAGAAACCAATTGTCCATATTGCATCAGACATTGCCGTCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAGCATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAATCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGCATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGTTTCTCCATACCCGTAATACGACTCACTATAGGGTTTTGTTTAACTTTAAGAAGGAGATATACATACCCATGGGATCTGATAAAATTCATCATCATCATCATCACGTGTAATTAATTAACTTTAAGAAGGAGATATACATACCCGGGTAAGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCAGATCAATTCGCGCGCGAAGGCGAAGCGGCATGCATAATGTGCCTGTCAAATGGACGAAGCAGGGATTCTGCAAACCCTATGCTACTCCGTCAAGCCGTCAATTGTCTGATTCGTTACCAATTATGACAACTTGACGGCTACATCATTCACTTTTTCTTCACAACCGGCACGGAACTCGCTCGGGCTGGCCCCGGTGCATTTTTTAAATACCCGCGAGAAATAGAGTTGATCGTCAAAACCAACATTGCGACCGACGGTGGCGATAGGCATCCGGGTGGTGCTCAAAAGCAGCTTCGCCTGGCTGATACGTTGGTCCTCGCGCCAGCTTAAGACGCTAATCCCTAACTGCTGGCGGAAAAGATGTGACAGACGCGACGGCGACAAGCAAACATGCTGTGCGACGCTGGCGATATCAAAATTGCTGTCTGCCAGGTGATCGCTGATGTACTGACAAGCCTCGCGTACCCGATTATCCATCGGTGGATGGAGCGACTCGTTAATCGCTTCCATGCGCCGCAGTAACAATTGCTCAAGCAGATTTATCGCCAGCAGCTCCGAATAGCGCCCTTCCCCTTGCCCGGCGTTAATGATTTGCCCAAACAGGTCGCTGAAATGCGGCTGGTGCGCTTCATCCGGGCGAAAGAACCCCGTATTGGCAAATATTGACGGCCAGTTAAGCCATTCATGCCAGTAGGCGCGCGGACGAAAGTAAACCCACTGGTGATACCATTCGCGAGCCTCCGGATGACGACCGTAGTGATGAATCTCTCCTGGCGGGAACAGCAAAATATCACCCGGTCGGCAAACAAATTCTCGTCCCTGATTTTTCACCACCCCCTGACCGCGAATGGTGAGATTGAGAATATAACCTTTCATTCCCAGCGGTCGGTCGATAAAAAAATCGAGATAACCGTTGGCCTCAATCGGCGTTAAACCCGCCACCAGATGGGCATTAAACGAGTATCCCGGCAGCAGGGGATCATTTTGCGCTTCAGCCATACTTTTCATACTCCCGCCATTCAGAG

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

ColEI origin of replication

1921

2603

promoter

T7 promoter

290

310

promoter

araC

araC promoter

256

285

selectable marker

AmpR

ampicillin resistance gene

1164

1823

tag

His

N-terminal 6x His tag

367

381

trxn regulatory element

arabinose O1 operator

161

182

trxn regulatory element

arabinose O2 operator

Gene

Gene Symbol :

TM0276

GENEID :

897191

Locus Tag :

TM0276

Target GenBank:

NP_228089

Protein

Structural Annotation Wiki :

TOPSAN

Swissprot ID :

Q9WYB3

Clone

Cloning Information :

276277

HIP Master Clone ID :

205763

Original Clone ID :

8070

Experimental

TargetTrack - Experimental data :

282153

Organism

Species Specific ID:

897191