Gene:
Gene Symbol:
TM1518
Gene Name:
aspartate kinase
Original Clone ID:
8283
Keyword:
None
Species:
Thermotoga maritima
Type:
cDNA
Vector Name:
pMH2T7
Format:
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Joint Center for Structural Genomics
Joint Center for Structural Genomics
TmCD00282218
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 1206nts
Open reading frame: 37 to 1242
ATGGGATCTGATAAAATTCATCATCATCATCATCACATGAACGTGGTGGTTCAAAAATAC
GGAGGAAGCTCCGTTGCCACACCGGAGAGAATAAAGAACGTCGCGCAGAGAATAAAAAAG
AAAGTGGACGAAGGGTACAAAGTTGTGGTCGTTGTCTCCGCTATGGGAAAGACAACTGAC
AACCTCATAAAACTCGCAAAGGAGATCTCTCCAAGACCGGATTCGAGAGAACTCGATATG
CTTCTTGCAACGGGAGAACAGGTTTCCGCCGCGCTCCTTTCGATGGCCCTGAAAGATCTC
GGAGTAAAGGCCAAATCTTTGAACGCGTTCCAGGTGAAAATAAAAACCACTCCGCATCAC
ACGAGCGCTCGTATCGTGGATATAGATGACAGTGTGATCCGGGAAAACCTGAAAGATTAC
GATGTTCTCGTCGTAACGGGCTTTCAGGGTGTGAACGAACACGGTGATCTGACCACACTC
GGTCGGGGCGGTTCGGATACATCTGCCGTGGCGCTTGCGGCAAAACTCAGAGTGCCGTGT
GAAATCTACAGCGACGTGGACGGGATATACACCTGCGATCCGCGTGTCCATCCGAGAGCC
AAAAAGCTCGCTTACATCACCTACGACGAAGCTCTCGAGCTTACCGCTCTCGGTGCGAAA
GTTCTTCACTCAAGATCCGTGGAAATAGCAAAGAAGTATGGGATACCCATCTACTGTGCA
TCTTCTTTCACCGAAGAGGAGGGAACCATGGTGGTAGAAAGACTTCCGGAGTGGCTGGAA
GAACCCGTTGTAACAGGAGCGACAATTTCTCATGGTCAAATAAAAGTTTCCATATCTTTC
CTCCCAAAGGATGTGAAGTACATCACGGCCATTTTCGAGGAAGTTGGAAAAAGGGCTCTG
AACGTGGACATGATCTCCCTTGTCCCTTCGAACGGAAAGGTTTTCCTCTCCTTCACGATT
CTCGAAGACCACAAAGAAGATCTGGACGAAGCGCTCAAAGAGGCTCTCAGGGATGTGGAA
GGGTGGAAATCCACCTACGAAGGAGGTTTTGCCAAACTCTCCATAGTTGGAGTGGGAATG
AGAACGAGTCCGGGCGTGGCCGCAAGGTTTTTTGAAGCGCTGGAAAGAGCGGGAGTCACA
CCGGAGCTCGTCACAACGTCGGAGATAAAGATCTCCTGTCTTGTTCCTGAAGAGAAAGCG
GAAGAGGCGCTGAAATCGGTGATCGAAGAATTCGAACTGTAATTAA
Protein sequence predicted based on nucleotide sequence
MNVVVQKYGGSSVATPERIKNVAQRIKKKVDEGYKVVVVVSAMGKTTDNLIKLAKEISPR PDSRELDMLLATGEQVSAALLSMALKDLGVKAKSLNAFQVKIKTTPHHTSARIVDIDDSV IRENLKDYDVLVVTGFQGVNEHGDLTTLGRGGSDTSAVALAAKLRVPCEIYSDVDGIYTC DPRVHPRAKKLAYITYDEALELTALGAKVLHSRSVEIAKKYGIPIYCASSFTEEEGTMVV ERLPEWLEEPVVTGATISHGQIKVSISFLPKDVKYITAIFEEVGKRALNVDMISLVPSNG KVFLSFTILEDHKEDLDEALKEALRDVEGWKSTYEGGFAKLSIVGVGMRTSPGVAARFFE ALERAGVTPELVTTSEIKISCLVPEEKAEEALKSVIEEFEL*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
1206
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in 283375
Recommended expression in:
Not Applicable
Vector Name:
pMH2T7
Synonyms:
pMH-2-T7
Sequencing Primer:
Forward:
pBAD forward
Reverse:
alt_bad_rev
Description:
Bacterial (E. coli) expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHH tag; amp resistance; restriction enzyme (Pml I/Pac I) or PIPE cloning.
Comments:
None
Size (bp):
4084
Parent Vector:
None
Properties:
PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker
Author Name:
Joint Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
ColEI origin of replication
1921
2603
promoter
T7
T7 promoter
290
310
promoter
araC
araC promoter
256
285
selectable marker
AmpR
ampicillin resistance gene
1164
1823
tag
His
N-terminal 6x His tag
367
381
trxn regulatory element
arabinose O1 operator
arabinose O1 operator
161
182
trxn regulatory element
arabinose O2 operator
arabinose O2 operator
5
23
