Gene:
Gene Symbol:
TM0236
Gene Name:
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanyl ligase
Original Clone ID:
20053
Keyword:
partial cds
Species:
Thermotoga maritima
Type:
cDNA
Vector Name:
pMH1TEV
Format:
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
Joint Center for Structural Genomics
Joint Center for Structural Genomics
TmCD00282485
Price:
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 1257nts
Open reading frame: 51 to 1307
CTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCTTCGTTCTGA
ATTCGAAAGAAGTGAGAGAAGGTGACGTGTTCGTCGCCGTGAAAGGGAAAAGATTCGATG
GTCACGATTTTATAGATGAAGCCCTCAGGAACGGCGCTTATGCGATCATTGCCGAGAGAA
AAACGGTGAATTCTGATAGAATCTTTCTCGTAGAAAGTTCCGTTGACACGCTTGCTAAGC
TGGCCCGAGAAAAGCTCGGGAATTTTTCCGGTACAGTCGTTGGTGTAACAGGTTCATCGG
GAAAAACCACCACGAAAGAAATATTGTACAACCTTCTCAAAAACAAGAGAAGTGTCTTCA
AAACACCGGGAAACATGAACACGGAGTACGGGTTGCCACTCTCCATTCTCAACGACTACA
AAGGAGAAGAAATCCTGGTTCTCGAAATGGCCGCGAGCAGGCCCGGTGATATCGCTCACC
TGTGCAAAATCGCTCCTCCGGATGTCGCCGTTCTCCTCAACGTTGGAAGCGCTCACCTCG
AGTTTTTTGGAACACGAGAAAGAATCATGGAAACCAAGATGGAGATCATCAAGCACTCGA
AAGAAAATGCGATAGCCGTAACACTCTTTGACGATCCTGATCTGAGAAAAGAAGTGCCTC
GCTACAGAAATACACTGTTCTTCGGCAAAGAAGGCGGTGACTCGGTTCTAAAGGATTGGT
GGTACTATGAGGGTTCCACGATCGCAGAGTTCGAAGCTTTTGATTCTCTTTTCACAGTGA
AACTCTCCGGATACTGGAATGGAGGGCAACTTCTGAACATCGCCGCTTCTCTGTGTGTCA
TGAGAACTTTGGGCGAAACTGTGGATATCTTCGATCTTGCCAGCCTGAAAACAGTTCCCG
GCCGTTTCAACGTGAGAGAAAAAAAAGGTGTGCTGATCGTGGATGATACGTACAACGCAA
GCCCTGAAGCGTTTCAAACTTCCATAGAGGCGCTTCTTCGTTTCCCCGGAAAGAAATTCG
CTGTTGTGGGAGCGATGAAAGAGCTCGGGGAGAGGTCGAAGGAGTTTCACGAAGAACTGG
GAGAGAGATTGAACGTTCTGGATGGGGTGTATGTTTTCTTGTCAGAACCCGAAGCAGAAT
GGATCAAGTCCAAAAAGATCATCCTGAAGTCAGACGATCCCGAAAAGATAGCGAAAGATC
TCGCCACCAGGGTAAAAAAAGGGGATGTGGTTCTCTTCAAAGCTTCAAGAGCGGTGAGAA
TAGAAAGAGTTCTGGAAATGTTTGAAAAGGAGCTGGAGAAAAGATAAGTTTAAACGGTCT
CCAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTT
Protein sequence predicted based on nucleotide sequence
FVLNSKEVREGDVFVAVKGKRFDGHDFIDEALRNGAYAIIAERKTVNSDRIFLVESSVDT LAKLAREKLGNFSGTVVGVTGSSGKTTTKEILYNLLKNKRSVFKTPGNMNTEYGLPLSIL NDYKGEEILVLEMAASRPGDIAHLCKIAPPDVAVLLNVGSAHLEFFGTRERIMETKMEII KHSKENAIAVTLFDDPDLRKEVPRYRNTLFFGKEGGDSVLKDWWYYEGSTIAEFEAFDSL FTVKLSGYWNGGQLLNIAASLCVMRTLGETVDIFDLASLKTVPGRFNVREKKGVLIVDDT YNASPEAFQTSIEALLRFPGKKFAVVGAMKELGERSKEFHEELGERLNVLDGVYVFLSEP EAEWIKSKKIILKSDDPEKIAKDLATRVKKGDVVLFKASRAVRIERVLEMFEKELEKR*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
1257
Mutations:
None
Discrepancy:
t435c,C145C;
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Protein_Purified
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in 360278
Recommended expression in:
Not Applicable
Vector Name:
pMH1TEV
Synonyms:
pMH-1-TEV
Sequencing Primer:
Forward:
pBAD forward
Reverse:
alt_bad_rev
Description:
Bacterial expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHHENLYFQG tag; amp resistance; PIPE cloning.
Comments:
None
Size (bp):
4069
Parent Vector:
None
Properties:
PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker
Author Name:
Joint Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
ColE1-type bacterial origin of replication
1908
2590
inducible trxn activator protein gene
araC
araC cds
3163
4035
promoter
araC
araC promoter
256
285
protease cleavage site
TEV
TEV protease cleavage site
382
402
selectable marker
AmpR
ampicillin resistance gene
1151
1810
tag
His
N-terminal 6xHis tag
364
381
trxn regulatory element
arabinose O1 operator
arabinose O1 operator
5
23
trxn regulatory element
arabinose O2 operator
arabinose O2 operator
161
182
trxn termination sequence
rrnB
rrnB T2 transcriptional terminator
813
839
