DNASU Plasmid | DULLARD (H. sapiens ) in pCR4zeroBLUNT-TOPO (TOPO cloning vector)

Explanation of Terms

Gene:

Gene Symbol: CTDNEP1

Gene Name: CTD nuclear envelope phosphatase 1

Original Clone ID:

8669a1ZBt1

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pCR4zeroBLUNT-TOPO

Format:

CLOSED

Source:

New York SGX Research Center for Structural Genomics

Description:

None

Comments:

"Invitrogen Zero Blunt, TOPO"

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

PSI
New York SGX Research Center for Structural Genomics

HsCD00285682

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 663nts

Open reading frame: 51 to 713

Reference Sequence Alignment

TACGACTCACTATAGGGCGAATTGAATTTAGCGGCCGCGAATTCGCCCTTTTCATTTACC
TTCTGCGGAGGCAGATCCGCACGGTAATTCAGTACCAAACTGTTCGATATGATATCCTCC
CCTTATCTCCTGTGTCCCGGAATCGGCTAGCCCAGGTGAAGAGGAAGATCCTGGTGCTGG
ATCTGGATGAGACACTTATTCACTCCCACCATGATGGGGTCCTGAGGCCCACAGTCCGGC
CTGGTACGCCTCCTGACTTCATCCTCAAGGTGGTAATAGACAAACATCCTGTCCGGTTTT
TTGTACATAAGAGGCCCCATGTGGATTTCTTCCTGGAAGTGGTGAGCCAGTGGTACGAGC
TGGTGGTGTTTACAGCAAGCATGGAGATCTATGGCTCTGCTGTGGCAGATAAACTGGACA
ATAGCAGAAGCATTCTTAAGAGGAGATATTACAGACAGCACTGCACTTTGGAGTTGGGCA
GCTACATCAAGGACCTCTCTGTGGTCCACAGTGACCTCTCCAGCATTGTGATCCTGGATA
ACTCCCCAGGGGCTTACAGGAGCCATCCAGACAATGCCATCCCCATCAAATCCTGGTTCA
GTGACCCCAGCGACACAGCCCTTCTCAACCTGCTCCCAATGCTGGATGCCCTCAGGTTCA
CCGCTGATGTTCGTTCCGTGCTGAGCCGAAACCTTCACCAACATCGGCTCTGAAGGGCGA
ATTCGTTTAAACCTGCAGGACTAGTCCCTTTAGTGAGGGTTA

Protein sequence predicted based on nucleotide sequence

FIYLLRRQIRTVIQYQTVRYDILPLSPVSRNRLAQVKRKILVLDLDETLIHSHHDGVLRP
TVRPGTPPDFILKVVIDKHPVRFFVHKRPHVDFFLEVVSQWYELVVFTASMEIYGSAVAD
KLDNSRSILKRRYYRQHCTLELGSYIKDLSVVHSDLSSIVILDNSPGAYRSHPDNAIPIK
SWFSDPSDTALLNLLPMLDALRFTADVRSVLSRNLHQHRL*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

Start on reference sequence 1

End on reference sequence 663

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin
Host Type: bacterial Marker: kanamycin

Bacterial Selection Condition: 50 ug/mL ampicillin

Growth Condition: Growth at 37 degrees C in LB supplemented with ampicillin (50 ug/mL)

Comments: None

Protein Expression Results:

ProteinExpressed: Not_Applicable

ProteinPurified: Not_Applicable

SolubleProtein: Not_Applicable

Find experimental protocols in NYSGXRC-8669a

Recommended expression in:

Not Applicable

Vector Name:

pCR4zeroBLUNT-TOPO

Synonyms:

pCR-4, pCR-IV, pCR4, pCR4-TOPO

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

TOPO cloning vector with M13 forward/reverse, T3, and T7 primers. Ampicillin and kanamycin resistance bacteria.

Comments:

Empty vector is a product available from Invitrogen.

Size (bp):

3956

Parent Vector:

None

Properties:

TOPO Cloning

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

AGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTCAGAATTAACCCTCACTAAAGGGACTAGTCCTGCAGGTTTAAACGAATTCGCCCTTAAGGGCGAATTCGCGGCCGCTAAATTCAATTCGCCCTATAGTGAGTCGTATTACAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTATACGTACGGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACACGCCGGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAAATGTCAGGCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTCACGTAGAAAGCCAGTCCGCAGAAACGGTGCTGACCCCGGATGAATGTCAGCTACTGGGCTATCTGGACAAGGGAAAACGCAAGCGCAAAGAGAAAGCAGGTAGCTTGCAGTGGGCTTACATGGCGATAGCTAGACTGGGCGGTTTTATGGACAGCAAGCGAACCGGAATTGCCAGCTGGGGCGCCCTCTGGTAAGGTTGGGAAGCCCTGCAAAGTAAACTGGATGGCTTTCTTGCCGCCAAGGATCTGATGGCGCAGGGGATCAAGCTCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAAGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGAGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAATTATTAACGCTTACAATTTCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAG

Vector Features:

Type

Name

Description

Start

End

bacterial origin

pUC ori

pUC bacterial origin of replication

3161

3834

primer site

M13 reverse

M13 reverse priming site

205

221

primer site

T3 priming site

243

262

primer site

M13 forward

M13 forward priming site

355

370

primer site

T7 priming site

328

347

promoter

lac

lac promoter region

216

selectable marker

AmpR

Ampicillin resistance gene

2203

3063

selectable marker

KanR

Kanamycin resistance gene

1159

1953

Gene

Gene Symbol :

CTDNEP1

Symbol Nomenclature :

CTDNEP1

SYNONYM :

DULLARD; HSA011916; NET56

Designation :

C-terminal domain nuclear envelope phosphatase 1|dullard homolog|serine/threonine-protein phosphatase dullard

Full Nomenclature :

CTD nuclear envelope phosphatase 1

GENEID :

23399

HGNC :

19085

MIM :

610684

Vega :

OTTHUMG00000102180

Target GenBank:

NP_056158

Reference Sequence Alignment

  

HsCD00285682        -------------------TACGACTCACTATAGGGCGAATTGAATTTAGCGGCCGCGAA
NM_001143775.1      ATGATGCGGACGCAGTGTCTGCTGGGGCTGCGCACGTTCGTGGCCTTCGCCGCCAAGCTC
NM_015343.4         ATGATGCGGACGCAGTGTCTGCTGGGGCTGCGCACGTTCGTGGCCTTCGCCGCCAAGCTC
                                       *.* .   .  . .. *  ..* *..** . ** *..  :.

HsCD00285682        TTCGCCCTTTTCATTTACCTTCTGCGGAGGCAGATCCGCACGGTAATTCAGTACCAAACT
NM_001143775.1      TGGAGCTTCTTCATTTACCTTCTGCGGAGGCAGATCCGCACGGTAATTCAGTACCAAACT
NM_015343.4         TGGAGCTTCTTCATTTACCTTCTGCGGAGGCAGATCCGCACGGTAATTCAGTACCAAACT
                    *  . * * ***************************************************

HsCD00285682        GTTCGATATGATATCCTCCCCTTATCTCCTGTGTCCCGGAATCGGCTAGCCCAGGTGAAG
NM_001143775.1      GTTCGATATGATATCCTCCCCTTATCTCCTGTGTCCCGGAATCGGCTAGCCCAGGTGAAG
NM_015343.4         GTTCGATATGATATCCTCCCCTTATCTCCTGTGTCCCGGAATCGGCTAGCCCAGGTGAAG
                    ************************************************************

HsCD00285682        AGGAAGATCCTGGTGCTGGATCTGGATGAGACACTTATTCACTCCCACCATGATGGGGTC
NM_001143775.1      AGGAAGATCCTGGTGCTGGATCTGGATGAGACACTTATTCACTCCCACCATGATGGGGTC
NM_015343.4         AGGAAGATCCTGGTGCTGGATCTGGATGAGACACTTATTCACTCCCACCATGATGGGGTC
                    ************************************************************

HsCD00285682        CTGAGGCCCACAGTCCGGCCTGGTACGCCTCCTGACTTCATCCTCAAGGTGGTAATAGAC
NM_001143775.1      CTGAGGCCCACAGTCCGGCCTGGTACGCCTCCTGACTTCATCCTCAAGGTGGTAATAGAC
NM_015343.4         CTGAGGCCCACAGTCCGGCCTGGTACGCCTCCTGACTTCATCCTCAAGGTGGTAATAGAC
                    ************************************************************

HsCD00285682        AAACATCCTGTCCGGTTTTTTGTACATAAGAGGCCCCATGTGGATTTCTTCCTGGAAGTG
NM_001143775.1      AAACATCCTGTCCGGTTTTTTGTACATAAGAGGCCCCATGTGGATTTCTTCCTGGAAGTG
NM_015343.4         AAACATCCTGTCCGGTTTTTTGTACATAAGAGGCCCCATGTGGATTTCTTCCTGGAAGTG
                    ************************************************************

HsCD00285682        GTGAGCCAGTGGTACGAGCTGGTGGTGTTTACAGCAAGCATGGAGATCTATGGCTCTGCT
NM_001143775.1      GTGAGCCAGTGGTACGAGCTGGTGGTGTTTACAGCAAGCATGGAGATCTATGGCTCTGCT
NM_015343.4         GTGAGCCAGTGGTACGAGCTGGTGGTGTTTACAGCAAGCATGGAGATCTATGGCTCTGCT
                    ************************************************************

HsCD00285682        GTGGCAGATAAACTGGACAATAGCAGAAGCATTCTTAAGAGGAGATATTACAGACAGCAC
NM_001143775.1      GTGGCAGATAAACTGGACAATAGCAGAAGCATTCTTAAGAGGAGATATTACAGACAGCAC
NM_015343.4         GTGGCAGATAAACTGGACAATAGCAGAAGCATTCTTAAGAGGAGATATTACAGACAGCAC
                    ************************************************************

HsCD00285682        TGCACTTTGGAGTTGGGCAGCTACATCAAGGACCTCTCTGTGGTCCACAGTGACCTCTCC
NM_001143775.1      TGCACTTTGGAGTTGGGCAGCTACATCAAGGACCTCTCTGTGGTCCACAGTGACCTCTCC
NM_015343.4         TGCACTTTGGAGTTGGGCAGCTACATCAAGGACCTCTCTGTGGTCCACAGTGACCTCTCC
                    ************************************************************

HsCD00285682        AGCATTGTGATCCTGGATAACTCCCCAGGGGCTTACAGGAGCCATCCAGACAATGCCATC
NM_001143775.1      AGCATTGTGATCCTGGATAACTCCCCAGGGGCTTACAGGAGCCATCCAGACAATGCCATC
NM_015343.4         AGCATTGTGATCCTGGATAACTCCCCAGGGGCTTACAGGAGCCATCCAGACAATGCCATC
                    ************************************************************

HsCD00285682        CCCATCAAATCCTGGTTCAGTGACCCCAGCGACACAGCCCTTCTCAACCTGCTCCCAATG
NM_001143775.1      CCCATCAAATCCTGGTTCAGTGACCCCAGCGACACAGCCCTTCTCAACCTGCTCCCAATG
NM_015343.4         CCCATCAAATCCTGGTTCAGTGACCCCAGCGACACAGCCCTTCTCAACCTGCTCCCAATG
                    ************************************************************

HsCD00285682        CTGGATGCCCTCAGGTTCACCGCTGATGTTCGTTCCGTGCTGAGCCGAAACCTTCACCAA
NM_001143775.1      CTGGATGCCCTCAGGTTCACCGCTGATGTTCGTTCCGTGCTGAGCCGAAACCTTCACCAA
NM_015343.4         CTGGATGCCCTCAGGTTCACCGCTGATGTTCGTTCCGTGCTGAGCCGAAACCTTCACCAA
                    ************************************************************

HsCD00285682        CATCGGCTCTGAAGGGCGAATTCGTTTAAACCTGCAGGACTAGTCCCTTTAGTGAGGGTT
NM_001143775.1      CATCGGCTCTGGTGA---------------------------------------------
NM_015343.4         CATCGGCTCTGGTGA---------------------------------------------
                    ***********.:*.                                             

HsCD00285682        A
NM_001143775.1      -
NM_015343.4         -