DNASU Plasmid | ALKBH8 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: ALKBH8

Gene Name: alkB, alkylation repair homolog 8 (E. coli)

Original Clone ID:

1709

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

HsCD00287659

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 717nts

Open reading frame: 1 to 717

Reference Sequence Alignment

ATGGACAGCAACCATCAAAGTAATTACAAACTCAGTAAAACTGAGAAGAAGTTCTTAAGG
AAACAGATTAAAGCCAAGCATACTTTGCTGAGACATGAAGGCATTGAGACAGTATCCTAT
GCCACTCAGAGCCTGGTTGTTGCCAATGGTGGTTTGGGTAATGGTGTGAGTCGGAACCAG
CTGCTCCCGGTTTTAGAGAAATGTGGACTGGTGGATGCTCTCTTAATGCCACCTAACAAG
CCGTACTCATTTGCAAGATACAGAACTACAGAAGAATCTAAGAGAGCCTATGTTACCCTC
AATGGAAAAGAAGTAGTGGATGATTTAGGACAAAAGATCACTCTGTATTTGAATTTTGTG
GAAAAAGTGCAGTGGAAGGAGTTGAGGCCTCAAGCCTTACCACCAGGACTCATGGTAGTA
GAAGAAATAATTTCTTCTGAGGAGGAGAAAATGCTTTTGGAAAGTGTTGATTGGACAGAA
GATACAGACAATCAAAACTCTCAAAAATCCTTAAAACACAGAAGAGTAAAGCATTTTGGT
TATGAGTTCCACTATGAGAACAACAATGTAGATAAAGATAAGCCATTATCTGGGGGTCTT
CCTGACATTTGTGAAAGCTTTTTGGAGAAATGGTTGAGGAAAGGTTACATTAAACATAAA
CCTGATCAAATGACCATAAATCAGTATGAACCTGGGCAAGATTGTCATGGATTTTAC

Protein sequence predicted based on nucleotide sequence

MDSNHQSNYKLSKTEKKFLRKQIKAKHTLLRHEGIETVSYATQSLVVANGGLGNGVSRNQ
LLPVLEKCGLVDALLMPPNKPYSFARYRTTEESKRAYVTLNGKEVVDDLGQKITLYLNFV
EKVQWKELRPQALPPGLMVVEEIISSEEEKMLLESVDWTEDTDNQNSQKSLKHRRVKHFG
YEFHYENNNVDKDKPLSGGLPDICESFLEKWLRKGYIKHKPDQMTINQYEPGQDCHGFY

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

Start on reference sequence 108

End on reference sequence 824

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Protein

Clone

Organism

Genome
Reagents

Gene

Gene Symbol :

ALKBH8

Symbol Nomenclature :

ALKBH8

SYNONYM :

ABH8

Designation :

AlkB homologue 8|S-adenosyl-L-methionine-dependent tRNA methyltransferase ABH8|alkylated DNA repair protein alkB homolog 8|probable alpha-ketoglutarate-dependent dioxygenase ABH8|tRNA (carboxymethyluridine(34)-5-O)-methyltransferase ABH8

Full Nomenclature :

alkB, alkylation repair homolog 8 (E. coli)

GENEID :

91801

HGNC :

25189

MIM :

613306

Vega :

OTTHUMG00000157008

Target GenBank:

BC015183.1

Reference Sequence Alignment

  

HsCD00287659        ------------------------------------------------------------
XM_005271727.1      ATGTTTGCTATGGACAGCAACCATCAAAGTAATTACAAACTCAGTAAAACTGAGAAGAAG
XM_005271729.1      ------------------------------------------------------------
XM_005271732.1      ------------------------------------------------------------
XM_005271728.1      ------------------------------------------------------------
NM_138775.2         ---------ATGGACAGCAACCATCAAAGTAATTACAAACTCAGTAAAACTGAGAAGAAG
                                                                                

HsCD00287659        ------------------------------------------------------------
XM_005271727.1      TTCTTAAGGAAACAGATTAAAGCCAAGCATACTTTGCTGAGACATGAAGGCATTGAGACA
XM_005271729.1      ------------------------------------------------------------
XM_005271732.1      ------------------------------------------------------------
XM_005271728.1      ------------------------------------------------------------
NM_138775.2         TTCTTAAGGAAACAGATTAAAGCCAAGCATACTTTGCTGAGACATGAAGGCATTGAGACA
                                                                                

HsCD00287659        ------------------------------------------------------------
XM_005271727.1      GTATCCTATGCCACTCAGAGCCTGGTTGTTGCCAATGGTGGTTTGGGTAATGGTGTGAGT
XM_005271729.1      ------------------------------------------------------------
XM_005271732.1      ------------------------------------------------------------
XM_005271728.1      ------------------------------------------------------------
NM_138775.2         GTATCCTATGCCACTCAGAGCCTGGTTGTTGCCAATGGTGGTTTGGGTAATGGTGTGAGT
                                                                                

HsCD00287659        ------------------------------------------------------------
XM_005271727.1      CGGAACCAGCTGCTCCCGGTTTTAGAGAAATGTGGACTGGTGGATGCTCTCTTAATGCCA
XM_005271729.1      ------------------------------------------------------------
XM_005271732.1      ------------------------------------------------------------
XM_005271728.1      ------------------------------------------------------------
NM_138775.2         CGGAACCAGCTGCTCCCGGTTTTAGAGAAATGTGGACTGGTGGATGCTCTCTTAATGCCA
                                                                                

HsCD00287659        ------------------------------------------------------------
XM_005271727.1      CCTAACAAGCCGTACTCATTTGCAAGATACAGAACTACAGAAGAATCTAAGAGAGCCTAT
XM_005271729.1      ------------------------------------------------------------
XM_005271732.1      ------------------------------------------------------------
XM_005271728.1      ------------------------------------------------------------
NM_138775.2         CCTAACAAGCCGTACTCATTTGCAAGATACAGAACTACAGAAGAATCTAAGAGAGCCTAT
                                                                                

HsCD00287659        ------------------------------------------------------------
XM_005271727.1      GTTACCCTCAATGGAAAAGAAGTAGTGGATGATTTAGGACAAAAGATCACTCTGTATTTG
XM_005271729.1      ------------------------------------------------------------
XM_005271732.1      ------------------------------------------------------------
XM_005271728.1      ------------------------------------------------------------
NM_138775.2         GTTACCCTCAATGGAAAAGAAGTAGTGGATGATTTAGGACAAAAGATCACTCTGTATTTG
                                                                                

HsCD00287659        ------------------------------------------------------------
XM_005271727.1      AATTTTGTGGAAAAAGTGCAGTGGAAGGAGTTGAGGCCTCAAGCCTTACCACCAGGACTC
XM_005271729.1      ------------------------------------------------------------
XM_005271732.1      ------------------------------------------------------------
XM_005271728.1      ------------------------------------------------------------
NM_138775.2         AATTTTGTGGAAAAAGTGCAGTGGAAGGAGTTGAGGCCTCAAGCCTTACCACCAGGACTC
                                                                                

HsCD00287659        ------------------------------------------------------------
XM_005271727.1      ATGGTAGTAGAAGAAATAATTTCTTCTGAGGAGGAGAAAATGCTTTTGGAAAGTGTTGAT
XM_005271729.1      ATGGTAGTAGAAGAAATAATTTCTTCTGAGGAGGAGAAAATGCTTTTGGAAAGTGTTGAT
XM_005271732.1      ------------------------------------------------------------
XM_005271728.1      ATGGTAGTAGAAGAAATAATTTCTTCTGAGGAGGAGAAAATGCTTTTGGAAAGTGTTGAT
NM_138775.2         ATGGTAGTAGAAGAAATAATTTCTTCTGAGGAGGAGAAAATGCTTTTGGAAAGTGTTGAT
                                                                                

HsCD00287659        ----------------------------------------------------ATGGACAG
XM_005271727.1      TGGACAGAAGATACAGACAATCAAAACTCTCAAAAATCCTTAAAACACAGAAGAGTAAAG
XM_005271729.1      TGGACAGAAGATACAGACAATCAAAACTCTCAAAAATCCTTAAAACACAGAAGAGTAAAG
XM_005271732.1      -------------------------------------------ATGTTTGCTATGGACAG
XM_005271728.1      TGGACAGAAGATACAGACAATCAAAACTCTCAAAAATCCTTAAAACACAGAAGAGTAAAG
NM_138775.2         TGGACAGAAGATACAGACAATCAAAACTCTCAAAAATCCTTAAAACACAGAAGAGTAAAG
                                                                        .:* *.**

HsCD00287659        CAACCATCAAAGTAATTACAAACTCAGTAAAACTGAGAAGAAGTTCTTAAGGAAACAGAT
XM_005271727.1      CATTTTGGTTATGAGTTCCACTATGAGAACAACAATGTAGATAAAGATAAGCCATTATCT
XM_005271729.1      CATTTTGGTTATGAGTTCCACTATGAGAACAACAATGTAGATAAAGATAAGCCATTATCT
XM_005271732.1      CAACCATCAAAGTAATTACAAACTCAGTAAAACTGAGAAGAAGTTCTTAAGGAAACAGAT
XM_005271728.1      CATTTTGGTTATGAGTTCCACTATGAGAACAACAATGTAGATAAAGATAAGCCATTATCT
NM_138775.2         CATTTTGGTTATGAGTTCCACTATGAGAACAACAATGTAGATAAAGATAAGCCATTATCT
                    **:  :  ::*  *.**.**.:.* **:*.***:.:*:***:.:: :**** .*: * .*

HsCD00287659        TAAAGCCAAGCATACTTTGCTGAGACATGAAGGCATTGAGACAGTATCCTATGCCACTCA
XM_005271727.1      GGGGGTCTTCCTGACATTTGTGAAAGCTTTTTGGAGAAATGGTTGAGGAAAGGTTACATT
XM_005271729.1      GGGGGTCTTCCTGACATTTGTGAAAGCTTTTTGGAGAAATGGTTGAGGAAAGGTTACATT
XM_005271732.1      TAAAGCCAAGCATACTTTGCTGAGACATGAAGGCATTGAGACAGTATCCTATGCCACTCA
XM_005271728.1      GGGGGTCTTCCTGACATTTGTGAAAGCTTTTTGGAGAAATGGTTGAGGAAAGGTTACATT
NM_138775.2         GGGGGTCTTCCTGACATTTGTGAAAGCTTTTTGGAGAAATGGTTGAGGAAAGGTTACATT
                     ...* *:: *: **:**  ***.* .* :: * * :.* . :  *  .:* *  **: :

HsCD00287659        GAGCCTGGTTGTTGCCAATGGTGGTTTGGGTAATGG------------------------
XM_005271727.1      AAACATAAACCTGATCAAATGACCATAAATCAGTATGAACCTGGGCAAGGAATTCCCGCT
XM_005271729.1      AAACATAAACCTGATCAAATGACCATAAATCAGTATGAACCTGGGCAAGGAATTCCCGCT
XM_005271732.1      GAGCCTGGTTGTTGCCAATGGTGGTTTGGGTAATGG------------------------
XM_005271728.1      AAACATAAACCTGATCAAATGACCATAAATCAGTATGAACCTGGGCAAGGAATTCCCGCT
NM_138775.2         AAACATAAACCTGATCAAATGACCATAAATCAGTATGAACCTGGGCAAGGAATTCCCGCT
                    .*.*.*..:  * . ***: *:  :*:..  *.*.                         

HsCD00287659        ------------------------------------------------------------
XM_005271727.1      CATATTGATACACATTCCGCTTTTGAGGATGAGATCGTTTCTCTCAGTTTGGGGTCAGAG
XM_005271729.1      CATATTGATACACATTCCGCTTTTGAGGATGAGATCGTTTCTCTCAGTTTGGGGTCAGAG
XM_005271732.1      ------------------------------------------------------------
XM_005271728.1      CATATTGATACACATTCCGCTTTTGAGGATGAGATCGTTTCTCTCAGTTTGGGGTCAGAG
NM_138775.2         CATATTGATACACATTCCGCTTTTGAGGATGAGATCGTTTCTCTCAGTTTGGGGTCAGAG
                                                                                

HsCD00287659        ---------TGTGAGTCGGAACCAGCTGCTCCCGGTTTTAGAGAAATGTGGACTGGTGGA
XM_005271727.1      ATTGTCATGGATTTTAAGCACCCAGATGGCATTGCAGTGCCAGTTATGTTGCCTCGTCGG
XM_005271729.1      ATTGTCATGGATTTTAAGCACCCAGATGGCATTGCAGTGCCAGTTATGTTGCCTCGTCGG
XM_005271732.1      ---------TGTGAGTCGGAACCAGCTGCTCCCGGTTTTAGAGAAATGTGGACTGGTGGA
XM_005271728.1      ATTGTCATGGATTTTAAGCACCCAGATGGCATTGCAGTGCCAGTTATGTTGCCTCGTCGG
NM_138775.2         ATTGTCATGGATTTTAAGCACCCAGATGGCATTGCAGTGCCAGTTATGTTGCCTCGTCGG
                              .* : :.* *.****.**  .  * : * . **::**** *.** ** *.

HsCD00287659        TGCTCTCTTAATGCCACCTAACAAGCCGTACTCATTTGCAAGATACAGAACTACAGAAGA
XM_005271727.1      AGTTTGCTGGTGATGACAGGAGAATCTAGATACCTTTGGACCCATGGAATCACGTGCAGA
XM_005271729.1      AGTTTGCTGGTGATGACAGGAGAATCTAGATACCTTTGGACCCATGGAATCACGTGCAGA
XM_005271732.1      TGCTCTCTTAATGCCACCTAACAAGCCGTACTCATTTGCAAGATACAGAACTACAGAAGA
XM_005271728.1      AGTTTGCTGGTGATGACAGGAGAATCTAGATACCTTTGGACCCATGGAATCACGTGCAGA
NM_138775.2         AGTTTGCTGGTGATGACAGGAGAATCTAGATACCTTTGGACCCATGGAATCACGTGCAGA
                    :* *  ** .: .  **. .* ** * . * :*.**** *. .:: ..*:*:. :*.***

HsCD00287659        ATCTAAGAGAGCCTATGTTACCCTCAATGGAAAAGAAGTAGTGGATGATTTAGGACAAAA
XM_005271727.1      AAATTTGATACTGTTCAAGCATCTGAGAGTCTTAAAAGTGGAATTATCACCAGTGATGTT
XM_005271729.1      AAATTTGATACTGTTCAAGCATCTGAGAGTCTTAAAAGTGGAATTATCACCAGTGATGTT
XM_005271732.1      ATCTAAGAGAGCCTATGTTACCCTCAATGGAAAAGAAGTAGTGGATGATTTAGGACAAAA
XM_005271728.1      AAATTTGATACTGTTCAAGCATCTGAGAGTCTTAAAAGTGGAATTATCACCAGTGATGTT
NM_138775.2         AAATTTGATACTGTTCAAGCATCTGAGAGTCTTAAAAGTGGAATTATCACCAGTGATGTT
                    *:.*::** *   *: .: .. ** *.:* .::*.****.*:. :: .:  ** ..:.::

HsCD00287659        GATCACTCTGTATTTGAATTTTGTGGAAAAAGTGCAGTGGAAGGAGTTGAGGCCTCAAGC
XM_005271727.1      GGAGACTTAACTTTAAGCAAGAGGGGACTACGAACATCATTTACATTTAGGAAAGTGAGG
XM_005271729.1      GGAGACTTAACTTTAAGCAAGAGGGGACTACGAACATCATTTACATTTAGGAAAGTGAGG
XM_005271732.1      GATCACTCTGTATTTGAATTTTGTGGAAAAAGTGCAGTGGAAGGAGTTGAGGCCTCAAGC
XM_005271728.1      GGAGACTTAACTTTAAGCAAGAGGGGACTACGAACATCATTTACATTTAGGAAAGTGAGG
NM_138775.2         GGAGACTTAACTTTAAGCAAGAGGGGACTACGAACATCATTTACATTTAGGAAAGTGAGG
                    *.: *** :. :**:...:: :* ***.:*.*:.**  . ::. * **..*...  .** 

HsCD00287659        CTTACCACCAGGACTCATGGTAGTAGAAGAAATAATTTCTTCTGAGG-------------
XM_005271727.1      CAAACACCTTGTAACTGTAGTTACCCGTTGGTCTGTGATAGCCAGAGGAAAGAGACTCCC
XM_005271729.1      CAAACACCTTGTAACTGTAGTTACCCGTTGGTCTGTGATAGCCAGAGGAAAGAGACTCCC
XM_005271732.1      CTTACCACCAGGACTCATGGTAGTAGAAGAAATAATTTCTTCTGAGG-------------
XM_005271728.1      CAAACACCTTGTAACTGTAGTTACCCGTTGGTCTGTGATAGCCAGAGGAAAGAGACTCCC
NM_138775.2         CAAACACCTTGTAACTGTAGTTACCCGTTGGTCTGTGATAGCCAGAGGAAAGAGACTCCC
                    *::**..* :* *.  .*.**:. . .: ..: :.* : : * ...*             

HsCD00287659        ------------------------------------------------------------
XM_005271727.1      CCCTCATTTCCAGAGAGTGATAAAGAAGCCTCACGGCTGGAGCAAGAGTACGTCCATCAG
XM_005271729.1      CCCTCATTTCCAGAGAGTGATAAAGAAGCCTCACGGCTGGAGCAAGAGTACGTCCATCAG
XM_005271732.1      ------------------------------------------------------------
XM_005271728.1      CCCTCATTTCCAGAGAGTGATAAAGAAGCCTCACGGCTGGAGCAAGAGTACGTCCATCAG
NM_138775.2         CCCTCATTTCCAGAGAGTGATAAAGAAGCCTCACGGCTGGAGCAAGAGTACGTCCATCAG
                                                                                

HsCD00287659        ------------------------------------------------------------
XM_005271727.1      GTTTATGAAGAGATTGCTGGGCACTTCAGCAGCACAAGACATACCCCTTGGCCGCACATT
XM_005271729.1      GTTTATGAAGAGATTGCTGGGCACTTCAGCAGCACAAGACATACCCCTTGGCCGCACATT
XM_005271732.1      ------------------------------------------------------------
XM_005271728.1      GTTTATGAAGAGATTGCTGGGCACTTCAGCAGCACAAGACATACCCCTTGGCCGCACATT
NM_138775.2         GTTTATGAAGAGATTGCTGGGCACTTCAGCAGCACAAGACATACCCCTTGGCCGCACATT
                                                                                

HsCD00287659        ----------AGGAGAAAATGCTTTTGGAAAGTGTTGATTGGACAGAAGATACAGACAAT
XM_005271727.1      GTGGAGTTTTTGAAGGCTTTGCCAAGTGGTTCAATAGTGGCTGATATTGGATGTGGTAAT
XM_005271729.1      GTGGAGTTTTTGAAGGCTTTGCCAAGTGGTTCAATAGTGGCTGATATTGGATGTGGTAAT
XM_005271732.1      ----------AGGAGAAAATGCTTTTGGAAAGTGTTGATTGGACAGAAGATACAGACAAT
XM_005271728.1      GTGGAGTTTTTGAAGGCTTTGCCAAGTGGTTCAATAGTGGCTGATATTGGATGTGGTAAT
NM_138775.2         GTGGAGTTTTTGAAGGCTTTGCCAAGTGGTTCAATAGTGGCTGATATTGGATGTGGTAAT
                              :*.**..::*** ::  *.:: :.*:*:    ..:.::*.:: :*. ***

HsCD00287659        CAAAACTCTCAAAAATCCTTAAAACACAGAAGAGTAAAGCATTTTGGTTATGAGTTCCAC
XM_005271727.1      GGAAAGTATCTTGGCATCAATAAGGAGTTATATATGATTGGTTGTGATCGTAGCCAAAAC
XM_005271729.1      GGAAAGTATCTTGGCATCAATAAGGAGTTATATATGATTGGTTGTGATCGTAGCCAAAAC
XM_005271732.1      CAAAACTCTCAAAAATCCTTAAAACACAGAAGAGTAAAGCATTTTGGTTATGAGTTCCAC
XM_005271728.1      GGAAAGTATCTTGGCATCAATAAGGAGTTATATATGATTGGTTGTGATCGTAGCCAAAAC
NM_138775.2         GGAAAGTATCTTGGCATCAATAAGGAGTTATATATGATTGGTTGTGATCGTAGCCAAAAC
                     .*** *.**::...: *:::**. * : *:.:.*.*:  .** **.* .*..  :..**

HsCD00287659        TATGAGAACAACAATGTAGATAAAGATAAGCCATTATCTGGGGGTCTTCCTGACATTTGT
XM_005271727.1      CTTGTGGACATTTGTAGAGAGAGGCAATTTCAGGCTTTTGTCTGTGATGCATTGGCAGTA
XM_005271729.1      CTTGTGGACATTTGTAGAGAGAGGCAATTTCAGGCTTTTGTCTGTGATGCATTGGCAGTA
XM_005271732.1      TATGAGAACAACAATGTAGATAAAGATAAGCCATTATCTGGGGGTCTTCCTGACATTTGT
XM_005271728.1      CTTGTGGACATTTGTAGAGAGAGGCAATTTCAGGCTTTTGTCTGTGATGCATTGGCAGTA
NM_138775.2         CTTGTGGACATTTGTAGAGAGAGGCAATTTCAGGCTTTTGTCTGTGATGCATTGGCAGTA
                     :**:*.***: :.*. *** *.. *::: *..  :* **   ** :* *: : . :  :

HsCD00287659        GAAAGCTTTTTGGAGAAATGGTTGAGGAAAGGTTACATTAAACATAAACCTGATCAAATG
XM_005271727.1      CCAGTCCGCAGTGGGTCTTGTGATGCCTGCATCTCCATTGCTGTTATTCATCATTTTGCA
XM_005271729.1      CCAGTCCGCAGTGGGTCTTGTGATGCCTGCATCTCCATTGCTGTTATTCATCATTTTGCA
XM_005271732.1      GAAAGCTTTTTGGAGAAATGGTTGAGGAAAGGTTACATTAAACATAAACCTGATCAAATG
XM_005271728.1      CCAGTCCGCAGTGGGTCTTGTGATGCCTGCATCTCCATTGCTGTTATTCATCATTTTGCA
NM_138775.2         CCAGTCCGCAGTGGGTCTTGTGATGCCTGCATCTCCATTGCTGTTATTCATCATTTTGCA
                     .*. *   :  *.*:.:**  : .  :...  *.****..: :**::*.* ** ::. .

HsCD00287659        ACCATAAATCAGTATGAACCTGGGCAAGATTGTCATGGATTTTAC---------------
XM_005271727.1      ACAGCAGAGCGTAGAGTGGCAGCTCTCCAAGAAATTGTTCGACTCCTGAGACCAGGTGGG
XM_005271729.1      ACAGCAGAGCGTAGAGTGGCAGCTCTCCAAGAAATTGTTCGACTCCTGAGACCAGGTGGG
XM_005271732.1      ACCATAAATCAGTATGAACCTGGGCAAGGAATTCCCGCTCATATTGATACACATTCCGCT
XM_005271728.1      ACAGCAGAGCGTAGAGTGGCAGCTCTCCAAGAAATTGTTCGACTCCTGAGACCAGGTGGG
NM_138775.2         ACAGCAGAGCGTAGAGTGGCAGCTCTCCAAGAAATTGTTCGACTCCTGAGACCAGGTGGG
                    **.. *.* *. :.:*:. *:*  *:. .:  :.  * :  : :                

HsCD00287659        ------------------------------------------------------------
XM_005271727.1      AAGGCACTCATTTATGTCTGGGCAATGGAACAAGAATATAATAAGCAGAAGTCCAAGTAT
XM_005271729.1      AAGGCACTCATTTATGTCTGGGCAATGGAACAAGAATATAATAAGCAGAAGTCCAAGTAT
XM_005271732.1      TTTGAGGATGAGATCGTTTCTCTCAGTTTGGGGTCAGAGATTGTCATGGATTTTAAGCAC
XM_005271728.1      AAGGCACTCATTTATGTCTGGGCAATGGAACAAGAATATAATAAGCAGAAGTCCAAGTAT
NM_138775.2         AAGGCACTCATTTATGTCTGGGCAATGGAACAAGAATATAATAAGCAGAAGTCCAAGTAT
                                                                                

HsCD00287659        ------------------------------------------------------------
XM_005271727.1      CTTAGAGGAAACAGAAATAGCCAAGGAAAGAAAGAGGAGATGAACAGTGATACCTCAGTG
XM_005271729.1      CTTAGAGGAAACAGAAATAGCCAAGGAAAGAAAGAGGAGATGAACAGTGATACCTCAGTG
XM_005271732.1      CCAGATGGCATTGCAGTGCCAGTTATGTTGCCTCGTCGGAGTTTGCTGGTGATGACAGGA
XM_005271728.1      CTTAGAGGAAACAGAAATAGCCAAGGAAAGAAAGAGGAGATGAACAGTGATACCTCAGTG
NM_138775.2         CTTAGAGGAAACAGAAATAGCCAAGGAAAGAAAGAGGAGATGAACAGTGATACCTCAGTG
                                                                                

HsCD00287659        ------------------------------------------------------------
XM_005271727.1      CAGAGGTCACTTGTGGAGCAAATGCGTGACATGGGCAGTCGAGACTCGGCATCTTCTGTC
XM_005271729.1      CAGAGGTCACTTGTGGAGCAAATGCGTGACATGGGCAGTCGAGACTCGGCATCTTCTGTC
XM_005271732.1      GAATCTAGATACCTTTGGACCCATGGAATCACGTGCAGAAAATTTGATACTGTTCAAGCA
XM_005271728.1      CAGAGGTCACTTGTGGAGCAAATGCGTGACATGGGCAGTCGAGACTCGGCATCTTCTGTC
NM_138775.2         CAGAGGTCACTTGTGGAGCAAATGCGTGACATGGGCAGTCGAGACTCGGCATCTTCTGTC
                                                                                

HsCD00287659        ------------------------------------------------------------
XM_005271727.1      CCCCGCATTAATGACTCTCAGGAAGGAGGATGTAATTCAAGGCAAGTTTCTAATTCCAAG
XM_005271729.1      CCCCGCATTAATGACTCTCAGGAAGGAGGATGTAATTCAAGGCAAGTTTCTAATTCCAAG
XM_005271732.1      TCTGAGAGTCTTAAAAGTGGAATTATCACCAGTGATGTTGGAGACTTAACTTTAAGCAAG
XM_005271728.1      CCCCGCATTAATGACTCTCAGGAAGGAGGATGTAATTCAAGGCAAGTTTCTAATTCCAAG
NM_138775.2         CCCCGCATTAATGACTCTCAGGAAGGAGGATGTAATTCAAGGCAAGTTTCTAATTCCAAG
                                                                                

HsCD00287659        ------------------------------------------------------------
XM_005271727.1      CTGCCTGTTCATGTTAACAGGACTTCTTTTTATTCTCAAGATGTACTGGTTCCCTGGCAC
XM_005271729.1      CTGCCTGTTCATGTTAACAGGACTTCTTTTTATTCTCAAGATGTACTGGTTCCCTGGCAC
XM_005271732.1      AGGGGACTACGAACATCATTTACATTTAGGAAAGTGAGGCAAACACCTTGTAACTGTAAT
XM_005271728.1      CTGCCTGTTCATGTTAACAGGACTTCTTTTTATTCTCAAGATGTACTGGTTCCCTGGCAC
NM_138775.2         CTGCCTGTTCATGTTAACAGGACTTCTTTTTATTCTCAAGATGTACTGGTTCCCTGGCAC
                                                                                

HsCD00287659        ------------------------------------------------------------
XM_005271727.1      CTTAAGGGAAATCCTGATAAAGGCAAACCTGTTGAGCCATTTGGTCCCATAGGATCCCAG
XM_005271729.1      CTTAAGGGAAATCCTGATAAAGGCAAACCTGTTGAGCCATTTGGTCCCATAGGATCCCAG
XM_005271732.1      TGGTTGTGA---------------------------------------------------
XM_005271728.1      CTTAAGGGAAATCCTGATAAAGGCAAACCTGTTGAGCCATTTGGTCCCATAGGATCCCAG
NM_138775.2         CTTAAGGGAAATCCTGATAAAGGCAAACCTGTTGAGCCATTTGGTCCCATAGGATCCCAG
                                                                                

HsCD00287659        ------------------------------------------------------------
XM_005271727.1      GACCCAAGTCCTGTGTTTCATCGTTACTACCATGTGTTCCGTGAGGGAGAACTGGAAGGT
XM_005271729.1      GACCCAAGTCCTGTGTTTCATCGTTACTACCATGTGTTCCGTGAGGGAGAACTGGAAGGT
XM_005271732.1      ------------------------------------------------------------
XM_005271728.1      GACCCAAGTCCTGTGTTTCATCGTTACTACCATGTGTTCCGTGAGGGAGAACTGGAAGGT
NM_138775.2         GACCCAAGTCCTGTGTTTCATCGTTACTACCATGTGTTCCGTGAGGGAGAACTGGAAGGT
                                                                                

HsCD00287659        ------------------------------------------------------------
XM_005271727.1      GCCTGCAGGACTGTGAGTGATGTCAGAATTCTGCAAAGCTACTACGATCAAGGAAACTGG
XM_005271729.1      GCCTGCAGGACTGTGAGTGATGTCAGAATTCTGCAAAGCTACTACGATCAAGGAAACTGG
XM_005271732.1      ------------------------------------------------------------
XM_005271728.1      GCCTGCAGGACTGTGAGTGATGTCAGAATTCTGCAAAGCTACTACGATCAAGGAAACTGG
NM_138775.2         GCCTGCAGGACTGTGAGTGATGTCAGAATTCTGCAAAGCTACTACGATCAAGGAAACTGG
                                                                                

HsCD00287659        ------------------------
XM_005271727.1      TGTGTGATTCTTCAAAAGGCCTGA
XM_005271729.1      TGTGTGATTCTTCAAAAGGCCTGA
XM_005271732.1      ------------------------
XM_005271728.1      TGTGTGATTCTTCAAAAGGCCTGA
NM_138775.2         TGTGTGATTCTTCAAAAGGCCTGA