DNASU Plasmid | YIPF5 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: YIPF5

Gene Name: Yip1 domain family, member 5

Original Clone ID:

11141

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / Yes

Publications:

None

Authors:

The ORFeome Collaboration

HsCD00287730

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 774nts

Open reading frame: 1 to 774

Reference Sequence Alignment

ATGTCAGGCTTTGAAAAGTTAAACACGGATTTCTACCAGACAAGTTACAGCATCGATGAT
CAGTCACAGCAGTCCTATGATTATGGAGGAAGTGGAGGACCCTATAGCAAACAGTATGCT
GGCTATGACTATTCGCAGCAAGGCAGATTTGTCCCTCCAGACATGATGCAGCCACAACAG
CCATACACCGGGCAGATTTACCAGCCAACTCAGGCATATACTCCAGCTTCACCTCAGCCT
TTCTATGGAAACAACTTTGAGGATGAGCCACCTTTATTAGAAGAGTTAGGTATCAATTTT
GACCACATCTGGCAAAAAACACTAACAGTATTACATCCGTTAAAAGTAGCAGATGGCAGC
ATCATGAATGAAACTGATTTGGCAGGTCCAATGGTTTTTTGCCTTGCTTTTGGAGCCACA
TTGCTACTGGCTGGCAAAATCCAGTTTGGCTATGTATACGGGATCAGTGCAATTGGATGT
CTAGGAATGTTTTGTTTATTAAACTTAATGAGTATGACAGGTGTTTCATTTGGTTGTGTG
GCAAGTGTCCTTGGATATTGTCTTCTGCCCATGATCCTACTTTCCAGCTTTGCAGTGATA
TTTTCTTTGCAAGGAATGGTAGGAATCATTCTCACTGCTGGGATTATTGGATGGTGTAGT
TTTTCTGCTTCCAAAATATTTATTTCTGCATTAGCCATGGAAGGACAGCAACTTTTAGTA
GCATATCCTTGCGCTTTGTTATATGGAGTCTTTGCCCTGATTTCCGTCTTTTGC

Protein sequence predicted based on nucleotide sequence

MSGFEKLNTDFYQTSYSIDDQSQQSYDYGGSGGPYSKQYAGYDYSQQGRFVPPDMMQPQQ
PYTGQIYQPTQAYTPASPQPFYGNNFEDEPPLLEELGINFDHIWQKTLTVLHPLKVADGS
IMNETDLAGPMVFCLAFGATLLLAGKIQFGYVYGISAIGCLGMFCLLNLMSMTGVSFGCV
ASVLGYCLLPMILLSSFAVIFSLQGMVGIILTAGIIGWCSFSASKIFISALAMEGQQLLV
AYPCALLYGVFALISVFC

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

Start on reference sequence 126

End on reference sequence 899

Mutations:

None

Discrepancy:

c18g,N6K

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Protein

Clone

Organism

Genome
Reagents

Gene

Gene Symbol :

YIPF5

Symbol Nomenclature :

YIPF5

SYNONYM :

FinGER5; SB140; SMAP-5; SMAP5; YIP1A

Designation :

Full Nomenclature :

Yip1 domain family, member 5

GENEID :

81555

Locus Tag :

PP12723

HGNC :

24877

MIM :

611483

Vega :

OTTHUMG00000129679

Target GenBank:

BC024737.1

Reference Sequence Alignment

  

HsCD00287730        ATGTCAGGCTTTGAAAAGTTAAACACGGATTTCTACCAGACAAGTTACAGCATCGATGAT
NM_001024947.3      ATGTCAGGCTTTGAAAACTTAAACACGGATTTCTACCAGACAAGTTACAGCATCGATGAT
NM_030799.8         ATGTCAGGCTTTGAAAACTTAAACACGGATTTCTACCAGACAAGTTACAGCATCGATGAT
                    ***************** ******************************************

HsCD00287730        CAGTCACAGCAGTCCTATGATTATGGAGGAAGTGGAGGACCCTATAGCAAACAGTATGCT
NM_001024947.3      CAGTCACAGCAGTCCTATGATTATGGAGGAAGTGGAGGACCCTATAGCAAACAGTATGCT
NM_030799.8         CAGTCACAGCAGTCCTATGATTATGGAGGAAGTGGAGGACCCTATAGCAAACAGTATGCT
                    ************************************************************

HsCD00287730        GGCTATGACTATTCGCAGCAAGGCAGATTTGTCCCTCCAGACATGATGCAGCCACAACAG
NM_001024947.3      GGCTATGACTATTCGCAGCAAGGCAGATTTGTCCCTCCAGACATGATGCAGCCACAACAG
NM_030799.8         GGCTATGACTATTCGCAGCAAGGCAGATTTGTCCCTCCAGACATGATGCAGCCACAACAG
                    ************************************************************

HsCD00287730        CCATACACCGGGCAGATTTACCAGCCAACTCAGGCATATACTCCAGCTTCACCTCAGCCT
NM_001024947.3      CCATACACCGGGCAGATTTACCAGCCAACTCAGGCATATACTCCAGCTTCACCTCAGCCT
NM_030799.8         CCATACACCGGGCAGATTTACCAGCCAACTCAGGCATATACTCCAGCTTCACCTCAGCCT
                    ************************************************************

HsCD00287730        TTCTATGGAAACAACTTTGAGGATGAGCCACCTTTATTAGAAGAGTTAGGTATCAATTTT
NM_001024947.3      TTCTATGGAAACAACTTTGAGGATGAGCCACCTTTATTAGAAGAGTTAGGTATCAATTTT
NM_030799.8         TTCTATGGAAACAACTTTGAGGATGAGCCACCTTTATTAGAAGAGTTAGGTATCAATTTT
                    ************************************************************

HsCD00287730        GACCACATCTGGCAAAAAACACTAACAGTATTACATCCGTTAAAAGTAGCAGATGGCAGC
NM_001024947.3      GACCACATCTGGCAAAAAACACTAACAGTATTACATCCGTTAAAAGTAGCAGATGGCAGC
NM_030799.8         GACCACATCTGGCAAAAAACACTAACAGTATTACATCCGTTAAAAGTAGCAGATGGCAGC
                    ************************************************************

HsCD00287730        ATCATGAATGAAACTGATTTGGCAGGTCCAATGGTTTTTTGCCTTGCTTTTGGAGCCACA
NM_001024947.3      ATCATGAATGAAACTGATTTGGCAGGTCCAATGGTTTTTTGCCTTGCTTTTGGAGCCACA
NM_030799.8         ATCATGAATGAAACTGATTTGGCAGGTCCAATGGTTTTTTGCCTTGCTTTTGGAGCCACA
                    ************************************************************

HsCD00287730        TTGCTACTGGCTGGCAAAATCCAGTTTGGCTATGTATACGGGATCAGTGCAATTGGATGT
NM_001024947.3      TTGCTACTGGCTGGCAAAATCCAGTTTGGCTATGTATACGGGATCAGTGCAATTGGATGT
NM_030799.8         TTGCTACTGGCTGGCAAAATCCAGTTTGGCTATGTATACGGGATCAGTGCAATTGGATGT
                    ************************************************************

HsCD00287730        CTAGGAATGTTTTGTTTATTAAACTTAATGAGTATGACAGGTGTTTCATTTGGTTGTGTG
NM_001024947.3      CTAGGAATGTTTTGTTTATTAAACTTAATGAGTATGACAGGTGTTTCATTTGGTTGTGTG
NM_030799.8         CTAGGAATGTTTTGTTTATTAAACTTAATGAGTATGACAGGTGTTTCATTTGGTTGTGTG
                    ************************************************************

HsCD00287730        GCAAGTGTCCTTGGATATTGTCTTCTGCCCATGATCCTACTTTCCAGCTTTGCAGTGATA
NM_001024947.3      GCAAGTGTCCTTGGATATTGTCTTCTGCCCATGATCCTACTTTCCAGCTTTGCAGTGATA
NM_030799.8         GCAAGTGTCCTTGGATATTGTCTTCTGCCCATGATCCTACTTTCCAGCTTTGCAGTGATA
                    ************************************************************

HsCD00287730        TTTTCTTTGCAAGGAATGGTAGGAATCATTCTCACTGCTGGGATTATTGGATGGTGTAGT
NM_001024947.3      TTTTCTTTGCAAGGAATGGTAGGAATCATTCTCACTGCTGGGATTATTGGATGGTGTAGT
NM_030799.8         TTTTCTTTGCAAGGAATGGTAGGAATCATTCTCACTGCTGGGATTATTGGATGGTGTAGT
                    ************************************************************

HsCD00287730        TTTTCTGCTTCCAAAATATTTATTTCTGCATTAGCCATGGAAGGACAGCAACTTTTAGTA
NM_001024947.3      TTTTCTGCTTCCAAAATATTTATTTCTGCATTAGCCATGGAAGGACAGCAACTTTTAGTA
NM_030799.8         TTTTCTGCTTCCAAAATATTTATTTCTGCATTAGCCATGGAAGGACAGCAACTTTTAGTA
                    ************************************************************

HsCD00287730        GCATATCCTTGCGCTTTGTTATATGGAGTCTTTGCCCTGATTTCCGTCTTTTGC
NM_001024947.3      GCATATCCTTGCGCTTTGTTATATGGAGTCTTTGCCCTGATTTCCGTCTTTTGA
NM_030799.8         GCATATCCTTGCGCTTTGTTATATGGAGTCTTTGCCCTGATTTCCGTCTTTTGA
                    *****************************************************.