DNASU Plasmid | GOPC (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: GOPC

Gene Name: golgi-associated PDZ and coiled-coil motif containing

Original Clone ID:

34855

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

HsCD00287851

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1365nts

Open reading frame: 1 to 1365

Reference Sequence Alignment

ATGTCGGCGGGCGGTCCATGCCCAGCAGCAGCCGGAGGGGGCCCAGGGGGCGCCTCCTGC
TCCGTGGGGGCCCCTGGCGGGGTATCCATGTTCCGGTGGCTGGAGGTGCTGGAGAAGGAG
TTCGACAAAGCTTTTGTGGATGTGGATCTGCTCCTGGGAGAGATCGATCCAGACCAAGCG
GACATCACTTATGAGGGGCGACAGAAGATGACCAGCCTGAGCTCCTGCTTTGCACAGCTT
TGCCACAAAGCCCAGTCTGTGTCTCAAATCAACCACAAGCTGGAGGCACAGTTGGTGGAT
CTGAAATCTGAACTGACAGAAACCCAAGCAGAGAAAGTTGTTTTGGAGAAAGAAGTACAT
GATCAGCTTTTACAGCTGCACTCTATTCAGCTGCAGCTTCATGCTAAAACTGGTCAAAGT
GCTGACTCTGGTACCATTAAGGCAAAATTGGAAAGAGAGCTTGAGGCAAACAAAAAAGAA
AAAATGAAAGAAGCACAACTTGAAGCTGAAGTGAAATTGTTGAGAAAAGAGAATGAAGCC
CTTCGTAGACATATAGCTGTTCTCCAGGCTGAAGTATATGGGGCGAGACTAGCTGCCAAG
TACTTGGATAAGGAACTGGCAGGAAGGGTCCAACAGATACAATTGCTAGGACGAGATATG
AAGGGACCTGCTCATGATAAGCTTTGGAACCAATTAGAAGCTGAAATACATTTGCATCGT
CACAAAACTGTGATCCGAGCCTGCAGAGGACGTAATGACTTGAAACGACCAATGCAAGCA
CCACCAGGCCATGATCAAGATTCCCTAAAGAAAAGCCAAGGTGTTGGTCCAATTAGAAAA
GTTCTCCTCCTTAAGGAAGATCATGAAGGCCTTGGCATTTCAATTACAGGTGGGAAAGAA
CATGGTGTTCCAATCCTCATCTCTGAGATCCATCCGGGGCAACCTGCTGATAGATGCGGA
GGGCTGCACGTTGGGGATGCTATTTTGGCAGTCAACGGAGTTAACCTAAGGGACACAAAG
CATAAAGAAGCTGTAACTATTCTTTCTCAGCAGAGAGGAGAGATTGAATTTGAAGTAGTT
TATGTGGCTCCTGAAGTGGATTCTGATGATGAAAACGTAGAGTATGAAGATGAGAGTGGA
CATCGTTACCGTTTGTACCTTGATGAGTTAGAAGGAGGTGGTAACCCTGGTGCTAGTTGC
AAAGACACAAGTGGGGAAATCAAAGTATTACAAGGATTTAATAAGAAGGCAGTAACTGAC
ACACATGAAAATGGAGACCTGGGCACTGCAAGTGAAACTCCGCTAGATGACGGTGCTTCA
AAATTAGATGATCTGCACACTCTGTATCATAAAAAATCTTATTAC

Protein sequence predicted based on nucleotide sequence

MSAGGPCPAAAGGGPGGASCSVGAPGGVSMFRWLEVLEKEFDKAFVDVDLLLGEIDPDQA
DITYEGRQKMTSLSSCFAQLCHKAQSVSQINHKLEAQLVDLKSELTETQAEKVVLEKEVH
DQLLQLHSIQLQLHAKTGQSADSGTIKAKLERELEANKKEKMKEAQLEAEVKLLRKENEA
LRRHIAVLQAEVYGARLAAKYLDKELAGRVQQIQLLGRDMKGPAHDKLWNQLEAEIHLHR
HKTVIRACRGRNDLKRPMQAPPGHDQDSLKKSQGVGPIRKVLLLKEDHEGLGISITGGKE
HGVPILISEIHPGQPADRCGGLHVGDAILAVNGVNLRDTKHKEAVTILSQQRGEIEFEVV
YVAPEVDSDDENVEYEDESGHRYRLYLDELEGGGNPGASCKDTSGEIKVLQGFNKKAVTD
THENGDLGTASETPLDDGASKLDDLHTLYHKKSYY

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

End on reference sequence 1554

Start on reference sequence 190

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

GOPC

Symbol Nomenclature :

GOPC

SYNONYM :

CAL; FIG; GOPC1; PIST; dJ94G16.2

Designation :

CFTR-associated ligand|Golgi associated PDZ and coiled-coil motif containing protein|Golgi-associated PDZ and coiled-coil motif-containing protein|PDZ protein interacting specifically with TC10|PDZ/coiled-coil domain binding partner for the rho-family GTPase TC10|dJ94G16.2 PIST|fused in glioblastoma|golgi-associated PDZ and coiled-coil motif containing protein

Full Nomenclature :

golgi-associated PDZ and coiled-coil motif containing

GENEID :

57120

HGNC :

17643

MIM :

606845

Vega :

OTTHUMG00000015457

Target GenBank:

BC009553.2

Reference Sequence Alignment

  

HsCD00287851        ATGTCGGCGGGCGGTCCATGCCCAGCAGCAGCCGGAGGGGGCCCAGGGGGCGCCTCCTGC
NM_001017408.2      ATGTCGGCGGGCGGTCCATGCCCAGCAGCAGCCGGAGGGGGCCCAGGGGGCGCCTCCTGC
NM_020399.3         ATGTCGGCGGGCGGTCCATGCCCAGCAGCAGCCGGAGGGGGCCCAGGGGGCGCCTCCTGC
                    ************************************************************

HsCD00287851        TCCGTGGGGGCCCCTGGCGGGGTATCCATGTTCCGGTGGCTGGAGGTGCTGGAGAAGGAG
NM_001017408.2      TCCGTGGGGGCCCCTGGCGGGGTATCCATGTTCCGGTGGCTGGAGGTGCTGGAGAAGGAG
NM_020399.3         TCCGTGGGGGCCCCTGGCGGGGTATCCATGTTCCGGTGGCTGGAGGTGCTGGAGAAGGAG
                    ************************************************************

HsCD00287851        TTCGACAAAGCTTTTGTGGATGTGGATCTGCTCCTGGGAGAGATCGATCCAGACCAAGCG
NM_001017408.2      TTCGACAAAGCTTTTGTGGATGTGGATCTGCTCCTGGGAGAGATCGATCCAGACCAAGCG
NM_020399.3         TTCGACAAAGCTTTTGTGGATGTGGATCTGCTCCTGGGAGAGATCGATCCAGACCAAGCG
                    ************************************************************

HsCD00287851        GACATCACTTATGAGGGGCGACAGAAGATGACCAGCCTGAGCTCCTGCTTTGCACAGCTT
NM_001017408.2      GACATCACTTATGAGGGGCGACAGAAGATGACCAGCCTGAGCTCCTGCTTTGCACAGCTT
NM_020399.3         GACATCACTTATGAGGGGCGACAGAAGATGACCAGCCTGAGCTCCTGCTTTGCACAGCTT
                    ************************************************************

HsCD00287851        TGCCACAAAGCCCAGTCTGTGTCTCAAATCAACCACAAGCTGGAGGCACAGTTGGTGGAT
NM_001017408.2      TGCCACAAAGCCCAGTCTGTGTCTCAAATCAACCACAAGCTGGAGGCACAGTTGGTGGAT
NM_020399.3         TGCCACAAAGCCCAGTCTGTGTCTCAAATCAACCACAAGCTGGAGGCACAGTTGGTGGAT
                    ************************************************************

HsCD00287851        CTGAAATCTGAACTGACAGAAACCCAAGCAGAGAAAGTTGTTTTGGAGAAAGAAGTACAT
NM_001017408.2      CTGAAATCTGAACTGACAGAAACCCAAGCAGAGAAAGTTGTTTTGGAGAAAGAAGTACAT
NM_020399.3         CTGAAATCTGAACTGACAGAAACCCAAGCAGAGAAAGTTGTTTTGGAGAAAGAAGTACAT
                    ************************************************************

HsCD00287851        GATCAGCTTTTACAGCTGCACTCTATTCAGCTGCAGCTTCATGCTAAAACTGGTCAAAGT
NM_001017408.2      GATCAGCTTTTACAGCTGCACTCTATTCAGCTGCAGCTTCATGCTAAAACTGGTCAAAGT
NM_020399.3         GATCAGCTTTTACAGCTGCACTCTATTCAGCTGCAGCTTCATGCTAAAACTGGTCAAAGT
                    ************************************************************

HsCD00287851        GCTGACTCTGGTACCATTAAGGCAAAATTG------------------------GAAAGA
NM_001017408.2      GCTGACTCTGGTACCATTAAGGCAAAATTG------------------------GAAAGA
NM_020399.3         GCTGACTCTGGTACCATTAAGGCAAAATTGTCTGGCCCCTCTGTGGAGGAGCTGGAAAGA
                    ******************************                        ******

HsCD00287851        GAGCTTGAGGCAAACAAAAAAGAAAAAATGAAAGAAGCACAACTTGAAGCTGAAGTGAAA
NM_001017408.2      GAGCTTGAGGCAAACAAAAAAGAAAAAATGAAAGAAGCACAACTTGAAGCTGAAGTGAAA
NM_020399.3         GAGCTTGAGGCAAACAAAAAAGAAAAAATGAAAGAAGCACAACTTGAAGCTGAAGTGAAA
                    ************************************************************

HsCD00287851        TTGTTGAGAAAAGAGAATGAAGCCCTTCGTAGACATATAGCTGTTCTCCAGGCTGAAGTA
NM_001017408.2      TTGTTGAGAAAAGAGAATGAAGCCCTTCGTAGACATATAGCTGTTCTCCAGGCTGAAGTA
NM_020399.3         TTGTTGAGAAAAGAGAATGAAGCCCTTCGTAGACATATAGCTGTTCTCCAGGCTGAAGTA
                    ************************************************************

HsCD00287851        TATGGGGCGAGACTAGCTGCCAAGTACTTGGATAAGGAACTGGCAGGAAGGGTCCAACAG
NM_001017408.2      TATGGGGCGAGACTAGCTGCCAAGTACTTGGATAAGGAACTGGCAGGAAGGGTCCAACAG
NM_020399.3         TATGGGGCGAGACTAGCTGCCAAGTACTTGGATAAGGAACTGGCAGGAAGGGTCCAACAG
                    ************************************************************

HsCD00287851        ATACAATTGCTAGGACGAGATATGAAGGGACCTGCTCATGATAAGCTTTGGAACCAATTA
NM_001017408.2      ATACAATTGCTAGGACGAGATATGAAGGGACCTGCTCATGATAAGCTTTGGAACCAATTA
NM_020399.3         ATACAATTGCTAGGACGAGATATGAAGGGACCTGCTCATGATAAGCTTTGGAACCAATTA
                    ************************************************************

HsCD00287851        GAAGCTGAAATACATTTGCATCGTCACAAAACTGTGATCCGAGCCTGCAGAGGACGTAAT
NM_001017408.2      GAAGCTGAAATACATTTGCATCGTCACAAAACTGTGATCCGAGCCTGCAGAGGACGTAAT
NM_020399.3         GAAGCTGAAATACATTTGCATCGTCACAAAACTGTGATCCGAGCCTGCAGAGGACGTAAT
                    ************************************************************

HsCD00287851        GACTTGAAACGACCAATGCAAGCACCACCAGGCCATGATCAAGATTCCCTAAAGAAAAGC
NM_001017408.2      GACTTGAAACGACCAATGCAAGCACCACCAGGCCATGATCAAGATTCCCTAAAGAAAAGC
NM_020399.3         GACTTGAAACGACCAATGCAAGCACCACCAGGCCATGATCAAGATTCCCTAAAGAAAAGC
                    ************************************************************

HsCD00287851        CAAGGTGTTGGTCCAATTAGAAAAGTTCTCCTCCTTAAGGAAGATCATGAAGGCCTTGGC
NM_001017408.2      CAAGGTGTTGGTCCAATTAGAAAAGTTCTCCTCCTTAAGGAAGATCATGAAGGCCTTGGC
NM_020399.3         CAAGGTGTTGGTCCAATTAGAAAAGTTCTCCTCCTTAAGGAAGATCATGAAGGCCTTGGC
                    ************************************************************

HsCD00287851        ATTTCAATTACAGGTGGGAAAGAACATGGTGTTCCAATCCTCATCTCTGAGATCCATCCG
NM_001017408.2      ATTTCAATTACAGGTGGGAAAGAACATGGTGTTCCAATCCTCATCTCTGAGATCCATCCG
NM_020399.3         ATTTCAATTACAGGTGGGAAAGAACATGGTGTTCCAATCCTCATCTCTGAGATCCATCCG
                    ************************************************************

HsCD00287851        GGGCAACCTGCTGATAGATGCGGAGGGCTGCACGTTGGGGATGCTATTTTGGCAGTCAAC
NM_001017408.2      GGGCAACCTGCTGATAGATGCGGAGGGCTGCACGTTGGGGATGCTATTTTGGCAGTCAAC
NM_020399.3         GGGCAACCTGCTGATAGATGCGGAGGGCTGCACGTTGGGGATGCTATTTTGGCAGTCAAC
                    ************************************************************

HsCD00287851        GGAGTTAACCTAAGGGACACAAAGCATAAAGAAGCTGTAACTATTCTTTCTCAGCAGAGA
NM_001017408.2      GGAGTTAACCTAAGGGACACAAAGCATAAAGAAGCTGTAACTATTCTTTCTCAGCAGAGA
NM_020399.3         GGAGTTAACCTAAGGGACACAAAGCATAAAGAAGCTGTAACTATTCTTTCTCAGCAGAGA
                    ************************************************************

HsCD00287851        GGAGAGATTGAATTTGAAGTAGTTTATGTGGCTCCTGAAGTGGATTCTGATGATGAAAAC
NM_001017408.2      GGAGAGATTGAATTTGAAGTAGTTTATGTGGCTCCTGAAGTGGATTCTGATGATGAAAAC
NM_020399.3         GGAGAGATTGAATTTGAAGTAGTTTATGTGGCTCCTGAAGTGGATTCTGATGATGAAAAC
                    ************************************************************

HsCD00287851        GTAGAGTATGAAGATGAGAGTGGACATCGTTACCGTTTGTACCTTGATGAGTTAGAAGGA
NM_001017408.2      GTAGAGTATGAAGATGAGAGTGGACATCGTTACCGTTTGTACCTTGATGAGTTAGAAGGA
NM_020399.3         GTAGAGTATGAAGATGAGAGTGGACATCGTTACCGTTTGTACCTTGATGAGTTAGAAGGA
                    ************************************************************

HsCD00287851        GGTGGTAACCCTGGTGCTAGTTGCAAAGACACAAGTGGGGAAATCAAAGTATTACAAGGA
NM_001017408.2      GGTGGTAACCCTGGTGCTAGTTGCAAAGACACAAGTGGGGAAATCAAAGTATTACAAGGA
NM_020399.3         GGTGGTAACCCTGGTGCTAGTTGCAAAGACACAAGTGGGGAAATCAAAGTATTACAAGGA
                    ************************************************************

HsCD00287851        TTTAATAAGAAGGCAGTAACTGACACACATGAAAATGGAGACCTGGGCACTGCAAGTGAA
NM_001017408.2      TTTAATAAGAAGGCAGTAACTGACACACATGAAAATGGAGACCTGGGCACTGCAAGTGAA
NM_020399.3         TTTAATAAGAAGGCAGTAACTGACACACATGAAAATGGAGACCTGGGCACTGCAAGTGAA
                    ************************************************************

HsCD00287851        ACTCCGCTAGATGACGGTGCTTCAAAATTAGATGATCTGCACACTCTGTATCATAAAAAA
NM_001017408.2      ACTCCGCTAGATGACGGTGCTTCAAAATTAGATGATCTGCACACTCTGTATCATAAAAAA
NM_020399.3         ACTCCGCTAGATGACGGTGCTTCAAAATTAGATGATCTGCACACTCTGTATCATAAAAAA
                    ************************************************************

HsCD00287851        TCTTATTAC
NM_001017408.2      TCTTATTAA
NM_020399.3         TCTTATTAA
                    ********.