DNASU Plasmid | HYLS1 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: HYLS1

Gene Name: hydrolethalus syndrome 1

Original Clone ID:

28361

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

HsCD00287935

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 861nts

Open reading frame: 1 to 861

Reference Sequence Alignment

ATGGATCCAGAAGAACGAATGTTGGCAGCTGCTACAGCTTTTACCCACATCCGTGCAGGG
CAGGGTGAAGGAGATGTCAGGAGAGAAGCCCAATCTATCCAATATGATCCCTACAGTAAA
GCTTCAGTAGCCCCAGGGAAGCGACCTGCTCTTCCTGTGCAACTACAGTACCCACATGTA
GAAAGTAATGTCCCTTCAGAAACAGTCTCTGAGGCCTCCCAAAGACTCCGAAAGCCAGTG
ATGAAGAGAAAGGTGCTGCGCAGAAAGCCAGATGGGGAAGTATTAGTAACAGATGAGTCG
ATTATCAGTGAATCAGAATCTGGTACAGAAAATGATCAGGATCTCTGGGACTTAAGACAA
AGGCTGATGAATGTACAGTTCCAGGAAGACAAGGAATCTTCATTTGATGTTTCACAAAAA
TTTAACCTACCACATGAATACCAAGGAATTTCTCAAGATCAGCTCATTTGCTCTCTACAA
AGAGAAGGAATGGGCTCTCCAGCTTACGAACAAGACCTGATTGTTGCCAGCAGACCCAAG
TCCTTTATTCTCCCAAAGCTGGACCAGTTAAGCCGAAACCGGGGCAAGACAGACCGGGTA
GCCCGGTATTTTGAGTACAAACGGGACTGGGACTCAATACGTTTACCTGGTGAAGATCAT
AGAAAGGAATTACGCTGGGGTGTCCGAGAGCAGATGCTTTGTCGAGCAGAACCCCAATCC
AAACCTCAGCATATATATGTCCCAAACAATTATCTAGTACCAACAGAGAAGAAAAGGTCT
GCACTCCGTTGGGGTGTTCGTTGTGACCTTGCAAATGGTGTCATACCCAGGAAGCTTCCC
TTCCCTCTTTCTCCTTCTTAC

Protein sequence predicted based on nucleotide sequence

MDPEERMLAAATAFTHIRAGQGEGDVRREAQSIQYDPYSKASVAPGKRPALPVQLQYPHV
ESNVPSETVSEASQRLRKPVMKRKVLRRKPDGEVLVTDESIISESESGTENDQDLWDLRQ
RLMNVQFQEDKESSFDVSQKFNLPHEYQGISQDQLICSLQREGMGSPAYEQDLIVASRPK
SFILPKLDQLSRNRGKTDRVARYFEYKRDWDSIRLPGEDHRKELRWGVREQMLCRAEPQS
KPQHIYVPNNYLVPTEKKRSALRWGVRCDLANGVIPRKLPFPLSPSY

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

End on reference sequence 1022

Start on reference sequence 162

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Protein

Clone

Organism

Genome
Reagents

Gene

Gene Symbol :

HYLS1

Symbol Nomenclature :

HYLS1

SYNONYM :

HLS

Designation :

hydrolethalus syndrome protein 1

Full Nomenclature :

hydrolethalus syndrome 1

GENEID :

219844

HGNC :

26558

MIM :

610693

Vega :

OTTHUMG00000165858

Target GenBank:

BC015047.1

Reference Sequence Alignment

  

HsCD00287935        ---------------------------------------ATGGATCCAGAAGAACGAATG
NM_001134793.1      ATGGAAGAACTTCTACCTGATGGACAAATATGGGCTAATATGGATCCAGAAGAACGAATG
NM_145014.2         ATGGAAGAACTTCTACCTGATGGACAAATATGGGCTAATATGGATCCAGAAGAACGAATG
XM_005271430.1      ATGGAAGAACTTCTACCTGATGGACAAATATGGGCTAATATGGATCCAGAAGAACGAATG
                                                           *********************

HsCD00287935        TTGGCAGCTGCTACAGCTTTTACCCACATCCGTGCAGGGCAGGGTGAAGGAGATGTCAGG
NM_001134793.1      TTGGCAGCTGCTACAGCTTTTACCCACATCTGTGCAGGGCAGGGTGAAGGAGATGTCAGG
NM_145014.2         TTGGCAGCTGCTACAGCTTTTACCCACATCTGTGCAGGGCAGGGTGAAGGAGATGTCAGG
XM_005271430.1      TTGGCAGCTGCTACAGCTTTTACCCACATCTGTGCAGGGCAGGGTGAAGGAGATGTCAGG
                    ****************************** *****************************

HsCD00287935        AGAGAAGCCCAATCTATCCAATATGATCCCTACAGTAAAGCTTCAGTAGCCCCAGGGAAG
NM_001134793.1      AGAGAAGCCCAATCTATCCAATATGATCCCTACAGTAAAGCTTCAGTAGCCCCAGGGAAG
NM_145014.2         AGAGAAGCCCAATCTATCCAATATGATCCCTACAGTAAAGCTTCAGTAGCCCCAGGGAAG
XM_005271430.1      AGAGAAGCCCAATCTATCCAATATGATCCCTACAGTAAAGCTTCAGTAGCCCCAGGGAAG
                    ************************************************************

HsCD00287935        CGACCTGCTCTTCCTGTGCAACTACAGTACCCACATGTAGAAAGTAATGTCCCTTCAGAA
NM_001134793.1      CGACCTGCTCTTCCTGTGCAACTACAGTACCCACATGTAGAAAGTAATGTCCCTTCAGAA
NM_145014.2         CGACCTGCTCTTCCTGTGCAACTACAGTACCCACATGTAGAAAGTAATGTCCCTTCAGAA
XM_005271430.1      CGACCTGCTCTTCCTGTGCAACTACAGTACCCACATGTAGAAAGTAATGTCCCTTCAGAA
                    ************************************************************

HsCD00287935        ACAGTCTCTGAGGCCTCCCAAAGACTCCGAAAGCCAGTGATGAAGAGAAAGGTGCTGCGC
NM_001134793.1      ACAGTCTCTGAGGCCTCCCAAAGACTCCGAAAGCCAGTGATGAAGAGAAAGGTGCTGCGC
NM_145014.2         ACAGTCTCTGAGGCCTCCCAAAGACTCCGAAAGCCAGTGATGAAGAGAAAGGTGCTGCGC
XM_005271430.1      ACAGTCTCTGAGGCCTCCCAAAGACTCCGAAAGCCAGTGATGAAGAGAAAGGTGCTGCGC
                    ************************************************************

HsCD00287935        AGAAAGCCAGATGGGGAAGTATTAGTAACAGATGAGTCGATTATCAGTGAATCAGAATCT
NM_001134793.1      AGAAAGCCAGATGGGGAAGTATTAGTAACAGATGAGTCGATTATCAGTGAATCAGAATCT
NM_145014.2         AGAAAGCCAGATGGGGAAGTATTAGTAACAGATGAGTCGATTATCAGTGAATCAGAATCT
XM_005271430.1      AGAAAGCCAGATGGGGAAGTATTAGTAACAGATGAGTCGATTATCAGTGAATCAGAATCT
                    ************************************************************

HsCD00287935        GGTACAGAAAATGATCAGGATCTCTGGGACTTAAGACAAAGGCTGATGAATGTACAGTTC
NM_001134793.1      GGTACAGAAAATGATCAGGATCTCTGGGACTTAAGACAAAGGCTGATGAATGTACAGTTC
NM_145014.2         GGTACAGAAAATGATCAGGATCTCTGGGACTTAAGACAAAGGCTGATGAATGTACAGTTC
XM_005271430.1      GGTACAGAAAATGATCAGGATCTCTGGGACTTAAGACAAAGGCTGATGAATGTACAGTTC
                    ************************************************************

HsCD00287935        CAGGAAGACAAGGAATCTTCATTTGATGTTTCACAAAAATTTAACCTACCACATGAATAC
NM_001134793.1      CAGGAAGACAAGGAATCTTCATTTGATGTTTCACAAAAATTTAACCTACCACATGAATAC
NM_145014.2         CAGGAAGACAAGGAATCTTCATTTGATGTTTCACAAAAATTTAACCTACCACATGAATAC
XM_005271430.1      CAGGAAGACAAGGAATCTTCATTTGATGTTTCACAAAAATTTAACCTACCACATGAATAC
                    ************************************************************

HsCD00287935        CAAGGAATTTCTCAAGATCAGCTCATTTGCTCTCTACAAAGAGAAGGAATGGGCTCTCCA
NM_001134793.1      CAAGGAATTTCTCAAGATCAGCTCATTTGCTCTCTACAAAGAGAAGGAATGGGCTCTCCA
NM_145014.2         CAAGGAATTTCTCAAGATCAGCTCATTTGCTCTCTACAAAGAGAAGGAATGGGCTCTCCA
XM_005271430.1      CAAGGAATTTCTCAAGATCAGCTCATTTGCTCTCTACAAAGAGAAGGAATGGGCTCTCCA
                    ************************************************************

HsCD00287935        GCTTACGAACAAGACCTGATTGTTGCCAGCAGACCCAAGTCCTTTATTCTCCCAAAGCTG
NM_001134793.1      GCTTACGAACAAGACCTGATTGTTGCCAGCAGACCCAAGTCCTTTATTCTCCCAAAGCTG
NM_145014.2         GCTTACGAACAAGACCTGATTGTTGCCAGCAGACCCAAGTCCTTTATTCTCCCAAAGCTG
XM_005271430.1      GCTTACGAACAAGACCTGATTGTTGCCAGCAGACCCAAGTCCTTTATTCTCCCAAAGCTG
                    ************************************************************

HsCD00287935        GACCAGTTAAGCCGAAACCGGGGCAAGACAGACCGGGTAGCCCGGTATTTTGAGTACAAA
NM_001134793.1      GACCAGTTAAGCCGAAACCGGGGCAAGACAGACCGGGTAGCCCGGTATTTTGAGTACAAA
NM_145014.2         GACCAGTTAAGCCGAAACCGGGGCAAGACAGACCGGGTAGCCCGGTATTTTGAGTACAAA
XM_005271430.1      GACCAGTTAAGCCGAAACCGGGGCAAGACAGACCGGGTAGCCCGGTATTTTGAGTACAAA
                    ************************************************************

HsCD00287935        CGGGACTGGGACTCAATACGTTTACCTGGTGAAGATCATAGAAAGGAATTACGCTGGGGT
NM_001134793.1      CGGGACTGGGACTCAATACGTTTACCTGGTGAAGATCATAGAAAGGAATTACGCTGGGGT
NM_145014.2         CGGGACTGGGACTCAATACGTTTACCTGGTGAAGATCATAGAAAGGAATTACGCTGGGGT
XM_005271430.1      CGGGACTGGGACTCAATACGTTTACCTGGTGAAGATCATAGAAAGGAATTACGCTGGGGT
                    ************************************************************

HsCD00287935        GTCCGAGAGCAGATGCTTTGTCGAGCAGAACCCCAATCCAAACCTCAGCATATATATGTC
NM_001134793.1      GTCCGAGAGCAGATGCTTTGTCGAGCAGAACCCCAATCCAAACCTCAGCATATATATGTC
NM_145014.2         GTCCGAGAGCAGATGCTTTGTCGAGCAGAACCCCAATCCAAACCTCAGCATATATATGTC
XM_005271430.1      GTCCGAGAGCAGATGCTTTGTCGAGCAGAACCCCAATCCAAACCTCAGCATATATATGTC
                    ************************************************************

HsCD00287935        CCAAACAATTATCTAGTACCAACAGAGAAGAAAAGGTCTGCACTCCGTTGGGGTGTTCGT
NM_001134793.1      CCAAACAATTATCTAGTACCAACAGAGAAGAAAAGGTCTGCACTCCGTTGGGGTGTTCGT
NM_145014.2         CCAAACAATTATCTAGTACCAACAGAGAAGAAAAGGTCTGCACTCCGTTGGGGTGTTCGT
XM_005271430.1      CCAAACAATTATCTAGTACCAACAGAGAAGAAAAGGTCTGCACTCCGTTGGGGTGTTCGT
                    ************************************************************

HsCD00287935        TGTGACCTTGCAAATGGTGTCATACCCAGGAAGCTTCCCTTCCCTCTTTCTCCTTCTTAC
NM_001134793.1      TGTGACCTTGCAAATGGTGTCATACCCAGGAAGCTTCCCTTCCCTCTTTCTCCTTCTTAA
NM_145014.2         TGTGACCTTGCAAATGGTGTCATACCCAGGAAGCTTCCCTTCCCTCTTTCTCCTTCTTAA
XM_005271430.1      TGTGACCTTGCAAATGGTGTCATACCCAGGAAGCTTCCCTTCCCTCTTTCTCCTTCTTAA
                    ***********************************************************.