DNASU Plasmid | GPKOW (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: GPKOW

Gene Name: G patch domain and KOW motifs

Original Clone ID:

13693

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / Yes

Publications:

None

Authors:

The ORFeome Collaboration

HsCD00288451

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1431nts

Open reading frame: 1 to 1431

Reference Sequence Alignment

ATGGCTGACTCCAAAGAGGGTGTTTTGCCGCTGACGGCTGCTTCCACTGCCCCAATTTCA
TTCGGCTTCACTCGCACGTCCGCACGGAGGCGGCTGGCCGACTCGGGAGACGGCGCGGGG
CCATCTCCGGAGGAGAAGGATTTCTTGAAAACCGTGGAAGGGAGGGAGCTGCAGAGTGTG
AAGCCCCAGGAGGCCCCCAAGGAACTCGTCATCCCTTTGATCCAGAATGGCCATCGCAGG
CAGCCACCAGCCCGGCCCCCTGGGCCATCCACAGATACTGGGGCCTTGGCGGATGGGGTG
GTGTCCCAGGCTGTGAAGGAGCTCATTGCGGAATCCAAGAAGTCTCTGGAAGAGAGAGAG
AATGCGGGTGTCGACCCCACGCTCGCTATCCCCATGATCCAGAAAGGATGCACCCCCAGC
GGGGAAGGGGCAGACAGCGAACCCCGGGCAGAGACAGTGCCAGAGGAGGCTAATTATGAG
GCGGTCCCCGTGGAGGCCTATGGGCTGGCCATGCTGCGGGGCATGGGCTGGAAACCTGGC
GAGGGCATCGGCCGCACCTTCAATCAAGTAGTGAAGCCCCGTGTCAACTCACTGAGGCCC
AAGGGGTTAGGGCTGGGTGCCAACCTGACCGAAGCCCAGGCCTTGACCCCCACTGGCCCC
TCCCGCATGCCAAGACCAGATGAGGAGCAAGAGAAAGATAAGGAAGATCAGCCTCAAGGG
CTGGTGCCTGGAGGAGCTGTGGTGGTTCTTTCTGGCCCTCACCGAGGCCTCTATGGGAAG
GTGGAAGGCCTTGATCCTGACAATGTTCGGGCCATGGTTCGTCTGGCTGTGGGGAGCCGG
GTGGTGACTGTTAGTGAGTACTACCTGCGGCCTGTCTCCCAGCAGGAGTTTGACAAGAAC
ACCTTGGATCTCAGGCAACAGAACGGAACTGCCTCATCACGGAAGACCCTCTGGAATCAA
GAACTCTACATCCAGCAGGACAACTCAGAGAGGAAGCGGAAACACCTTCCAGACCGACAG
GATGGGCCTGCAGCCAAGAGTGAGAAAGCAGCCCCCAGAAGTCAGCACTGGTTGCACAGG
GACCTGCGTGTGCGGTTTGTGGACAACATGTACAAAGGAGGCCAATATTACAACACCAAG
ATGATAATTGAAGATGTCCTAAGCCCAGATACCTGTGTATGTCGGACAGATGAAGGCCGA
GTCCTGGAAGGCCTGAGGGAAGACATGCTGGAGACCCTGGTTCCCAAGGCAGAGGGTGAC
CGTGTGATGGTGGTGCTGGGCCCACAGACTGGAAGGGTGGGACATTTGCTGAGCCGGGAC
AGAGCACGGAGCCGGGCTTTGGTGCAACTGCCAAGAGAAAATCAGGTGGTGGAGCTTCAC
TACGATGCCATCTGCCAGTACATGGGCCCTAGTGACACAGATGATGACTGC

Protein sequence predicted based on nucleotide sequence

MADSKEGVLPLTAASTAPISFGFTRTSARRRLADSGDGAGPSPEEKDFLKTVEGRELQSV
KPQEAPKELVIPLIQNGHRRQPPARPPGPSTDTGALADGVVSQAVKELIAESKKSLEERE
NAGVDPTLAIPMIQKGCTPSGEGADSEPRAETVPEEANYEAVPVEAYGLAMLRGMGWKPG
EGIGRTFNQVVKPRVNSLRPKGLGLGANLTEAQALTPTGPSRMPRPDEEQEKDKEDQPQG
LVPGGAVVVLSGPHRGLYGKVEGLDPDNVRAMVRLAVGSRVVTVSEYYLRPVSQQEFDKN
TLDLRQQNGTASSRKTLWNQELYIQQDNSERKRKHLPDRQDGPAAKSEKAAPRSQHWLHR
DLRVRFVDNMYKGGQYYNTKMIIEDVLSPDTCVCRTDEGRVLEGLREDMLETLVPKAEGD
RVMVVLGPQTGRVGHLLSRDRARSRALVQLPRENQVVELHYDAICQYMGPSDTDDDC

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

End on reference sequence 1438

Start on reference sequence 8

Mutations:

None

Discrepancy:

g633a

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

GPKOW

Symbol Nomenclature :

GPKOW

SYNONYM :

GPATC5; GPATCH5; Spp2; T54

Designation :

G patch domain and KOW motifs-containing protein|protein MOS2 homolog

Full Nomenclature :

G patch domain and KOW motifs

GENEID :

27238

HGNC :

30677

Target GenBank:

BC000397.2

Reference Sequence Alignment

  

HsCD00288451      ATGGCTGACTCCAAAGAGGGTGTTTTGCCGCTGACGGCTGCTTCCACTGCCCCAATTTCA
NM_015698.5       ATGGCTGACTCCAAAGAGGGTGTTTTGCCGCTGACGGCTGCTTCCACTGCCCCAATTTCA
                  ************************************************************

HsCD00288451      TTCGGCTTCACTCGCACGTCCGCACGGAGGCGGCTGGCCGACTCGGGAGACGGCGCGGGG
NM_015698.5       TTCGGCTTCACTCGCACGTCCGCACGGAGGCGGCTGGCCGACTCGGGAGACGGCGCGGGG
                  ************************************************************

HsCD00288451      CCATCTCCGGAGGAGAAGGATTTCTTGAAAACCGTGGAAGGGAGGGAGCTGCAGAGTGTG
NM_015698.5       CCATCTCCGGAGGAGAAGGATTTCTTGAAAACCGTGGAAGGGAGGGAGCTGCAGAGTGTG
                  ************************************************************

HsCD00288451      AAGCCCCAGGAGGCCCCCAAGGAACTCGTCATCCCTTTGATCCAGAATGGCCATCGCAGG
NM_015698.5       AAGCCCCAGGAGGCCCCCAAGGAACTCGTCATCCCTTTGATCCAGAATGGCCATCGCAGG
                  ************************************************************

HsCD00288451      CAGCCACCAGCCCGGCCCCCTGGGCCATCCACAGATACTGGGGCCTTGGCGGATGGGGTG
NM_015698.5       CAGCCACCAGCCCGGCCCCCTGGGCCATCCACAGATACTGGGGCCTTGGCGGATGGGGTG
                  ************************************************************

HsCD00288451      GTGTCCCAGGCTGTGAAGGAGCTCATTGCGGAATCCAAGAAGTCTCTGGAAGAGAGAGAG
NM_015698.5       GTGTCCCAGGCTGTGAAGGAGCTCATTGCGGAATCCAAGAAGTCTCTGGAAGAGAGAGAG
                  ************************************************************

HsCD00288451      AATGCGGGTGTCGACCCCACGCTCGCTATCCCCATGATCCAGAAAGGATGCACCCCCAGC
NM_015698.5       AATGCGGGTGTCGACCCCACGCTCGCTATCCCCATGATCCAGAAAGGATGCACCCCCAGC
                  ************************************************************

HsCD00288451      GGGGAAGGGGCAGACAGCGAACCCCGGGCAGAGACAGTGCCAGAGGAGGCTAATTATGAG
NM_015698.5       GGGGAAGGGGCAGACAGCGAACCCCGGGCAGAGACAGTGCCAGAGGAGGCTAATTATGAG
                  ************************************************************

HsCD00288451      GCGGTCCCCGTGGAGGCCTATGGGCTGGCCATGCTGCGGGGCATGGGCTGGAAACCTGGC
NM_015698.5       GCGGTCCCCGTGGAGGCCTATGGGCTGGCCATGCTGCGGGGCATGGGCTGGAAACCTGGC
                  ************************************************************

HsCD00288451      GAGGGCATCGGCCGCACCTTCAATCAAGTAGTGAAGCCCCGTGTCAACTCACTGAGGCCC
NM_015698.5       GAGGGCATCGGCCGCACCTTCAATCAAGTAGTGAAGCCCCGTGTCAACTCACTGAGGCCC
                  ************************************************************

HsCD00288451      AAGGGGTTAGGGCTGGGTGCCAACCTGACCGAAGCCCAGGCCTTGACCCCCACTGGCCCC
NM_015698.5       AAGGGGTTAGGGCTGGGTGCCAACCTGACCGAGGCCCAGGCCTTGACCCCCACTGGCCCC
                  ********************************.***************************

HsCD00288451      TCCCGCATGCCAAGACCAGATGAGGAGCAAGAGAAAGATAAGGAAGATCAGCCTCAAGGG
NM_015698.5       TCCCGCATGCCAAGACCAGATGAGGAGCAAGAGAAAGATAAGGAAGATCAGCCTCAAGGG
                  ************************************************************

HsCD00288451      CTGGTGCCTGGAGGAGCTGTGGTGGTTCTTTCTGGCCCTCACCGAGGCCTCTATGGGAAG
NM_015698.5       CTGGTGCCTGGAGGAGCTGTGGTGGTTCTTTCTGGCCCTCACCGAGGCCTCTATGGGAAG
                  ************************************************************

HsCD00288451      GTGGAAGGCCTTGATCCTGACAATGTTCGGGCCATGGTTCGTCTGGCTGTGGGGAGCCGG
NM_015698.5       GTGGAAGGCCTTGATCCTGACAATGTTCGGGCCATGGTTCGTCTGGCTGTGGGGAGCCGG
                  ************************************************************

HsCD00288451      GTGGTGACTGTTAGTGAGTACTACCTGCGGCCTGTCTCCCAGCAGGAGTTTGACAAGAAC
NM_015698.5       GTGGTGACTGTTAGTGAGTACTACCTGCGGCCTGTCTCCCAGCAGGAGTTTGACAAGAAC
                  ************************************************************

HsCD00288451      ACCTTGGATCTCAGGCAACAGAACGGAACTGCCTCATCACGGAAGACCCTCTGGAATCAA
NM_015698.5       ACCTTGGATCTCAGGCAACAGAACGGAACTGCCTCATCACGGAAGACCCTCTGGAATCAA
                  ************************************************************

HsCD00288451      GAACTCTACATCCAGCAGGACAACTCAGAGAGGAAGCGGAAACACCTTCCAGACCGACAG
NM_015698.5       GAACTCTACATCCAGCAGGACAACTCAGAGAGGAAGCGGAAACACCTTCCAGACCGACAG
                  ************************************************************

HsCD00288451      GATGGGCCTGCAGCCAAGAGTGAGAAAGCAGCCCCCAGAAGTCAGCACTGGTTGCACAGG
NM_015698.5       GATGGGCCTGCAGCCAAGAGTGAGAAAGCAGCCCCCAGAAGTCAGCACTGGTTGCACAGG
                  ************************************************************

HsCD00288451      GACCTGCGTGTGCGGTTTGTGGACAACATGTACAAAGGAGGCCAATATTACAACACCAAG
NM_015698.5       GACCTGCGTGTGCGGTTTGTGGACAACATGTACAAAGGAGGCCAATATTACAACACCAAG
                  ************************************************************

HsCD00288451      ATGATAATTGAAGATGTCCTAAGCCCAGATACCTGTGTATGTCGGACAGATGAAGGCCGA
NM_015698.5       ATGATAATTGAAGATGTCCTAAGCCCAGATACCTGTGTATGTCGGACAGATGAAGGCCGA
                  ************************************************************

HsCD00288451      GTCCTGGAAGGCCTGAGGGAAGACATGCTGGAGACCCTGGTTCCCAAGGCAGAGGGTGAC
NM_015698.5       GTCCTGGAAGGCCTGAGGGAAGACATGCTGGAGACCCTGGTTCCCAAGGCAGAGGGTGAC
                  ************************************************************

HsCD00288451      CGTGTGATGGTGGTGCTGGGCCCACAGACTGGAAGGGTGGGACATTTGCTGAGCCGGGAC
NM_015698.5       CGTGTGATGGTGGTGCTGGGCCCACAGACTGGAAGGGTGGGACATTTGCTGAGCCGGGAC
                  ************************************************************

HsCD00288451      AGAGCACGGAGCCGGGCTTTGGTGCAACTGCCAAGAGAAAATCAGGTGGTGGAGCTTCAC
NM_015698.5       AGAGCACGGAGCCGGGCTTTGGTGCAACTGCCAAGAGAAAATCAGGTGGTGGAGCTTCAC
                  ************************************************************

HsCD00288451      TACGATGCCATCTGCCAGTACATGGGCCCTAGTGACACAGATGATGACTGC
NM_015698.5       TACGATGCCATCTGCCAGTACATGGGCCCTAGTGACACAGATGATGACTGA
                  **************************************************.