DNASU Plasmid | KLF10 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: KLF10

Gene Name: Kruppel-like factor 10

Original Clone ID:

4508

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / Yes

Publications:

None

Authors:

The ORFeome Collaboration

HsCD00288854

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1410nts

Open reading frame: 1 to 1410

Reference Sequence Alignment

ATGGAGGAAAGAATGGAAATGATTTCTGAAAGGCCAAAAGAGAGTATGTATTCCTGGAAC
AAAACTGCAGAGAAAAGTGATTTTGAAGCTGTAGAAGCACTTATGTCAATGAGCTGCAGT
TGGAAGTCTGATTTTAAGAAATACGTTGAAAACAGACCTGTTACACCAGTATCTGATTTG
TCAGAGGAAGAGAATCTGCTTCCGGGAACACCTGATTTTCATACAATCCCAGCATTTTGT
TTGACTCCACCTTACAGTCCTTCTGACTTTGAACCCTCTCAAGTGTCAAATCTGATGGCA
CCAGCGCCATCTACTGTACACTTCAAGTCACTCTCAGATACTGCCAAACCTCACATTGCC
GCACCTTTCAAAGAGGAAGAAAAGAGCCCAGTATCTGCCCCCAAACTCCCCAAAGCTCAG
GCAACAAGTGTGATTCGTCATACAGCTGATGCCCAGCTATGTAACCACCAGACCTGCCCA
ATGAAAGCAGCCAGCATCCTCAACTATCAGAACAATTCTTTTAGAAGAAGAACCCACCTA
AATGTTGAGGCTGCAAGAAAGAACATACCATGTGCCGCTGTGTCACCAAACAGATCCAAA
TGTGAGAGAAACACAGTGGCAGATGTTGATGAGAAAGCAAGTGCTGCACTTTATGACTTT
TCTGTGCCTTCCTCAGAGACGGTCATCTGCAGGTCTCAGCCAGCCCCTGTGTCCCCACAA
CAGAAGTCAGTGTTGGTCTCTCCACCTGCAGTATCTGCAGGGGGAGTGCCACCTATGCCG
GTCATCTGCCAGATGGTTCCCCTTCCTGCCAACAACCCTGTTGTGACAACAGTCGTTCCC
AGCACTCCTCCCAGCCAGCCACCAGCCGTTTGCCCCCCTGTTGTGTTCATGGGCACACAA
GTCCCCAAAGGCGCTGTCATGTTTGTGGTACCCCAGCCCGTTGTGCAGAGTTCAAAGCCT
CCGGTGGTGAGCCCGAATGGCACCAGACTCTCTCCCATTGCCCCTGCTCCTGGGTTTTCC
CCTTCAGCAGCAAAAGTCACTCCTCAGATTGATTCATCAAGGATAAGGAGTCACATCTGT
AGCCACCCAGGATGTGGCAAGACATACTTTAAAAGTTCCCATCTGAAGGCCCACACGAGG
ACGCACACAGGAGAAAAGCCTTTCAGCTGTAGCTGGAAAGGTTGTGAAAGGAGGTTTGCC
CGTTCTGATGAACTGTCCAGACACAGGCGAACCCACACGGGTGAGAAGAAATTTGCGTGC
CCCATGTGTGACCGGCGGTTCATGAGGAGTGACCATTTGACCAAGCATGCCCGGCGCCAT
CTATCAGCCAAGAAGCTACCAAACTGGCAGATGGAAGTGAGCAAGCTAAATGACATTGCT
CTACCTCCAACCCCTGCTCCCACTCAGTGC

Protein sequence predicted based on nucleotide sequence

MEERMEMISERPKESMYSWNKTAEKSDFEAVEALMSMSCSWKSDFKKYVENRPVTPVSDL
SEEENLLPGTPDFHTIPAFCLTPPYSPSDFEPSQVSNLMAPAPSTVHFKSLSDTAKPHIA
APFKEEEKSPVSAPKLPKAQATSVIRHTADAQLCNHQTCPMKAASILNYQNNSFRRRTHL
NVEAARKNIPCAAVSPNRSKCERNTVADVDEKASAALYDFSVPSSETVICRSQPAPVSPQ
QKSVLVSPPAVSAGGVPPMPVICQMVPLPANNPVVTTVVPSTPPSQPPAVCPPVVFMGTQ
VPKGAVMFVVPQPVVQSSKPPVVSPNGTRLSPIAPAPGFSPSAAKVTPQIDSSRIRSHIC
SHPGCGKTYFKSSHLKAHTRTHTGEKPFSCSWKGCERRFARSDELSRHRRTHTGEKKFAC
PMCDRRFMRSDHLTKHARRHLSAKKLPNWQMEVSKLNDIALPPTPAPTQC

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

End on reference sequence 1729

Start on reference sequence 320

Mutations:

None

Discrepancy:

a1404t

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

KLF10

Symbol Nomenclature :

KLF10

SYNONYM :

EGR-alpha; EGRA; TIEG; TIEG1

Designation :

Krueppel-like factor 10|TGFB-inducible early growth response protein 1|early growth response-alpha|transforming growth factor-beta-inducible early growth response protein 1|zinc finger transcription factor TIEG

Full Nomenclature :

Kruppel-like factor 10

GENEID :

7071

HGNC :

11810

MIM :

601878

Vega :

OTTHUMG00000164735

Target GenBank:

BC011538.1

Reference Sequence Alignment

  

HsCD00288854        ---------------------------------ATGGAGGAAAGAATGGAAATGATTTCT
NM_005655.3         ATGCTCAACTTCGGTGCCTCTCTCCAGCAGACTGCGGAGGAAAGAATGGAAATGATTTCT
NM_001032282.3      ---------------------------------ATGGAGGAAAGAATGGAAATGATTTCT
                                                     . *************************

HsCD00288854        GAAAGGCCAAAAGAGAGTATGTATTCCTGGAACAAAACTGCAGAGAAAAGTGATTTTGAA
NM_005655.3         GAAAGGCCAAAAGAGAGTATGTATTCCTGGAACAAAACTGCAGAGAAAAGTGATTTTGAA
NM_001032282.3      GAAAGGCCAAAAGAGAGTATGTATTCCTGGAACAAAACTGCAGAGAAAAGTGATTTTGAA
                    ************************************************************

HsCD00288854        GCTGTAGAAGCACTTATGTCAATGAGCTGCAGTTGGAAGTCTGATTTTAAGAAATACGTT
NM_005655.3         GCTGTAGAAGCACTTATGTCAATGAGCTGCAGTTGGAAGTCTGATTTTAAGAAATACGTT
NM_001032282.3      GCTGTAGAAGCACTTATGTCAATGAGCTGCAGTTGGAAGTCTGATTTTAAGAAATACGTT
                    ************************************************************

HsCD00288854        GAAAACAGACCTGTTACACCAGTATCTGATTTGTCAGAGGAAGAGAATCTGCTTCCGGGA
NM_005655.3         GAAAACAGACCTGTTACACCAGTATCTGATTTGTCAGAGGAAGAGAATCTGCTTCCGGGA
NM_001032282.3      GAAAACAGACCTGTTACACCAGTATCTGATTTGTCAGAGGAAGAGAATCTGCTTCCGGGA
                    ************************************************************

HsCD00288854        ACACCTGATTTTCATACAATCCCAGCATTTTGTTTGACTCCACCTTACAGTCCTTCTGAC
NM_005655.3         ACACCTGATTTTCATACAATCCCAGCATTTTGTTTGACTCCACCTTACAGTCCTTCTGAC
NM_001032282.3      ACACCTGATTTTCATACAATCCCAGCATTTTGTTTGACTCCACCTTACAGTCCTTCTGAC
                    ************************************************************

HsCD00288854        TTTGAACCCTCTCAAGTGTCAAATCTGATGGCACCAGCGCCATCTACTGTACACTTCAAG
NM_005655.3         TTTGAACCCTCTCAAGTGTCAAATCTGATGGCACCAGCGCCATCTACTGTACACTTCAAG
NM_001032282.3      TTTGAACCCTCTCAAGTGTCAAATCTGATGGCACCAGCGCCATCTACTGTACACTTCAAG
                    ************************************************************

HsCD00288854        TCACTCTCAGATACTGCCAAACCTCACATTGCCGCACCTTTCAAAGAGGAAGAAAAGAGC
NM_005655.3         TCACTCTCAGATACTGCCAAACCTCACATTGCCGCACCTTTCAAAGAGGAAGAAAAGAGC
NM_001032282.3      TCACTCTCAGATACTGCCAAACCTCACATTGCCGCACCTTTCAAAGAGGAAGAAAAGAGC
                    ************************************************************

HsCD00288854        CCAGTATCTGCCCCCAAACTCCCCAAAGCTCAGGCAACAAGTGTGATTCGTCATACAGCT
NM_005655.3         CCAGTATCTGCCCCCAAACTCCCCAAAGCTCAGGCAACAAGTGTGATTCGTCATACAGCT
NM_001032282.3      CCAGTATCTGCCCCCAAACTCCCCAAAGCTCAGGCAACAAGTGTGATTCGTCATACAGCT
                    ************************************************************

HsCD00288854        GATGCCCAGCTATGTAACCACCAGACCTGCCCAATGAAAGCAGCCAGCATCCTCAACTAT
NM_005655.3         GATGCCCAGCTATGTAACCACCAGACCTGCCCAATGAAAGCAGCCAGCATCCTCAACTAT
NM_001032282.3      GATGCCCAGCTATGTAACCACCAGACCTGCCCAATGAAAGCAGCCAGCATCCTCAACTAT
                    ************************************************************

HsCD00288854        CAGAACAATTCTTTTAGAAGAAGAACCCACCTAAATGTTGAGGCTGCAAGAAAGAACATA
NM_005655.3         CAGAACAATTCTTTTAGAAGAAGAACCCACCTAAATGTTGAGGCTGCAAGAAAGAACATA
NM_001032282.3      CAGAACAATTCTTTTAGAAGAAGAACCCACCTAAATGTTGAGGCTGCAAGAAAGAACATA
                    ************************************************************

HsCD00288854        CCATGTGCCGCTGTGTCACCAAACAGATCCAAATGTGAGAGAAACACAGTGGCAGATGTT
NM_005655.3         CCATGTGCCGCTGTGTCACCAAACAGATCCAAATGTGAGAGAAACACAGTGGCAGATGTT
NM_001032282.3      CCATGTGCCGCTGTGTCACCAAACAGATCCAAATGTGAGAGAAACACAGTGGCAGATGTT
                    ************************************************************

HsCD00288854        GATGAGAAAGCAAGTGCTGCACTTTATGACTTTTCTGTGCCTTCCTCAGAGACGGTCATC
NM_005655.3         GATGAGAAAGCAAGTGCTGCACTTTATGACTTTTCTGTGCCTTCCTCAGAGACGGTCATC
NM_001032282.3      GATGAGAAAGCAAGTGCTGCACTTTATGACTTTTCTGTGCCTTCCTCAGAGACGGTCATC
                    ************************************************************

HsCD00288854        TGCAGGTCTCAGCCAGCCCCTGTGTCCCCACAACAGAAGTCAGTGTTGGTCTCTCCACCT
NM_005655.3         TGCAGGTCTCAGCCAGCCCCTGTGTCCCCACAACAGAAGTCAGTGTTGGTCTCTCCACCT
NM_001032282.3      TGCAGGTCTCAGCCAGCCCCTGTGTCCCCACAACAGAAGTCAGTGTTGGTCTCTCCACCT
                    ************************************************************

HsCD00288854        GCAGTATCTGCAGGGGGAGTGCCACCTATGCCGGTCATCTGCCAGATGGTTCCCCTTCCT
NM_005655.3         GCAGTATCTGCAGGGGGAGTGCCACCTATGCCGGTCATCTGCCAGATGGTTCCCCTTCCT
NM_001032282.3      GCAGTATCTGCAGGGGGAGTGCCACCTATGCCGGTCATCTGCCAGATGGTTCCCCTTCCT
                    ************************************************************

HsCD00288854        GCCAACAACCCTGTTGTGACAACAGTCGTTCCCAGCACTCCTCCCAGCCAGCCACCAGCC
NM_005655.3         GCCAACAACCCTGTTGTGACAACAGTCGTTCCCAGCACTCCTCCCAGCCAGCCACCAGCC
NM_001032282.3      GCCAACAACCCTGTTGTGACAACAGTCGTTCCCAGCACTCCTCCCAGCCAGCCACCAGCC
                    ************************************************************

HsCD00288854        GTTTGCCCCCCTGTTGTGTTCATGGGCACACAAGTCCCCAAAGGCGCTGTCATGTTTGTG
NM_005655.3         GTTTGCCCCCCTGTTGTGTTCATGGGCACACAAGTCCCCAAAGGCGCTGTCATGTTTGTG
NM_001032282.3      GTTTGCCCCCCTGTTGTGTTCATGGGCACACAAGTCCCCAAAGGCGCTGTCATGTTTGTG
                    ************************************************************

HsCD00288854        GTACCCCAGCCCGTTGTGCAGAGTTCAAAGCCTCCGGTGGTGAGCCCGAATGGCACCAGA
NM_005655.3         GTACCCCAGCCCGTTGTGCAGAGTTCAAAGCCTCCGGTGGTGAGCCCGAATGGCACCAGA
NM_001032282.3      GTACCCCAGCCCGTTGTGCAGAGTTCAAAGCCTCCGGTGGTGAGCCCGAATGGCACCAGA
                    ************************************************************

HsCD00288854        CTCTCTCCCATTGCCCCTGCTCCTGGGTTTTCCCCTTCAGCAGCAAAAGTCACTCCTCAG
NM_005655.3         CTCTCTCCCATTGCCCCTGCTCCTGGGTTTTCCCCTTCAGCAGCAAAAGTCACTCCTCAG
NM_001032282.3      CTCTCTCCCATTGCCCCTGCTCCTGGGTTTTCCCCTTCAGCAGCAAAAGTCACTCCTCAG
                    ************************************************************

HsCD00288854        ATTGATTCATCAAGGATAAGGAGTCACATCTGTAGCCACCCAGGATGTGGCAAGACATAC
NM_005655.3         ATTGATTCATCAAGGATAAGGAGTCACATCTGTAGCCACCCAGGATGTGGCAAGACATAC
NM_001032282.3      ATTGATTCATCAAGGATAAGGAGTCACATCTGTAGCCACCCAGGATGTGGCAAGACATAC
                    ************************************************************

HsCD00288854        TTTAAAAGTTCCCATCTGAAGGCCCACACGAGGACGCACACAGGAGAAAAGCCTTTCAGC
NM_005655.3         TTTAAAAGTTCCCATCTGAAGGCCCACACGAGGACGCACACAGGAGAAAAGCCTTTCAGC
NM_001032282.3      TTTAAAAGTTCCCATCTGAAGGCCCACACGAGGACGCACACAGGAGAAAAGCCTTTCAGC
                    ************************************************************

HsCD00288854        TGTAGCTGGAAAGGTTGTGAAAGGAGGTTTGCCCGTTCTGATGAACTGTCCAGACACAGG
NM_005655.3         TGTAGCTGGAAAGGTTGTGAAAGGAGGTTTGCCCGTTCTGATGAACTGTCCAGACACAGG
NM_001032282.3      TGTAGCTGGAAAGGTTGTGAAAGGAGGTTTGCCCGTTCTGATGAACTGTCCAGACACAGG
                    ************************************************************

HsCD00288854        CGAACCCACACGGGTGAGAAGAAATTTGCGTGCCCCATGTGTGACCGGCGGTTCATGAGG
NM_005655.3         CGAACCCACACGGGTGAGAAGAAATTTGCGTGCCCCATGTGTGACCGGCGGTTCATGAGG
NM_001032282.3      CGAACCCACACGGGTGAGAAGAAATTTGCGTGCCCCATGTGTGACCGGCGGTTCATGAGG
                    ************************************************************

HsCD00288854        AGTGACCATTTGACCAAGCATGCCCGGCGCCATCTATCAGCCAAGAAGCTACCAAACTGG
NM_005655.3         AGTGACCATTTGACCAAGCATGCCCGGCGCCATCTATCAGCCAAGAAGCTACCAAACTGG
NM_001032282.3      AGTGACCATTTGACCAAGCATGCCCGGCGCCATCTATCAGCCAAGAAGCTACCAAACTGG
                    ************************************************************

HsCD00288854        CAGATGGAAGTGAGCAAGCTAAATGACATTGCTCTACCTCCAACCCCTGCTCCCACTCAG
NM_005655.3         CAGATGGAAGTGAGCAAGCTAAATGACATTGCTCTACCTCCAACCCCTGCTCCCACACAG
NM_001032282.3      CAGATGGAAGTGAGCAAGCTAAATGACATTGCTCTACCTCCAACCCCTGCTCCCACACAG
                    ********************************************************:***

HsCD00288854        TGC
NM_005655.3         TGA
NM_001032282.3      TGA
                    **.