DNASU Plasmid | SLC35E3 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: SLC35E3

Gene Name: solute carrier family 35, member E3

Original Clone ID:

35784

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

HsCD00289079

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 942nts

Open reading frame: 1 to 942

Reference Sequence Alignment

ATGGCATTGCTGGTGGACCGAGTGCGGGGCCACTGGCGAATCGCCGCCGGGCTCCTGTTC
AACCTGCTGGTGTCCATCTGCATTGTGTTCCTCAACAAATGGATTTATGTGTACCACGGC
TTCCCCAACATGAGCCTGACCCTGGTGCACTTCGTGGTCACCTGGCTGGGCTTGTATATC
TGCCAGAAGCTGGACATCTTTGCCCCCAAAAGTCTGCCGCCCTCCAGGCTCCTCCTCCTG
GCCCTCAGCTTCTGTGGCTTTGTGGTCTTCACTAACCTTTCTCTGCAGAACAACACCATA
GGCACCTATCAGCTGGCCAAGGCCATGACCACGCCGGTGATCATAGCCATCCAGACCTTC
TGCTACCAGAAAACCTTCTCCACCAGAATCCAGCTCACGCTGATTCCTATAACTTTAGGT
GTAATCCTAAATTCTTATTACGATGTGAAGTTTAATTTCCTTGGAATGGTGTTTGCTGCT
CTTGGTGTTTTAGTTACATCCCTTTATCAAGTGTGGGTAGGAGCCAAACAGCATGAATTA
CAAGTGAACTCAATGCAGCTGCTGTACTACCAGGCTCCGATGTCATCTGCCATGTTGCTG
GTTGCTGTGCCCTTCTTTGAGCCAGTGTTTGGAGAAGGAGGAATATTTGGTCCCTGGTCA
GTTTCTGCTTTGCTTATGGTGCTGCTATCTGGAGTAATAGCTTTCATGGTGAACTTATCA
ATTTATTGGATCATTGGGAACACTTCACCTGTCACCTATAACATGTTCGGACACTTCAAG
TTCTGCATTACTTTATTCGGAGGATATGTTTTATTTAAGGATCCACTGTCCATTAATCAG
GCCCTTGGCATTTTATGTACATTATTTGGCATTCTCGCCTATACCCACTTTAAGCTCAGT
GAACAGGAAGGAAGTAGGAGTAAACTGGCACAACGTCCTTAC

Protein sequence predicted based on nucleotide sequence

MALLVDRVRGHWRIAAGLLFNLLVSICIVFLNKWIYVYHGFPNMSLTLVHFVVTWLGLYI
CQKLDIFAPKSLPPSRLLLLALSFCGFVVFTNLSLQNNTIGTYQLAKAMTTPVIIAIQTF
CYQKTFSTRIQLTLIPITLGVILNSYYDVKFNFLGMVFAALGVLVTSLYQVWVGAKQHEL
QVNSMQLLYYQAPMSSAMLLVAVPFFEPVFGEGGIFGPWSVSALLMVLLSGVIAFMVNLS
IYWIIGNTSPVTYNMFGHFKFCITLFGGYVLFKDPLSINQALGILCTLFGILAYTHFKLS
EQEGSRSKLAQRPY

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

End on reference sequence 1132

Start on reference sequence 191

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

SLC35E3

Symbol Nomenclature :

SLC35E3

SYNONYM :

BLOV1

Designation :

bladder cancer overexpressed protein|bladder cancer-overexpressed gene 1 protein|solute carrier family 35 member E3|solute carrier family 35, member E2

Full Nomenclature :

solute carrier family 35, member E3

GENEID :

55508

Locus Tag :

UNQ3043/PRO9859

HGNC :

20864

Target GenBank:

BC030504.2

Reference Sequence Alignment

  

HsCD00289079      ATGGCATTGCTGGTGGACCGAGTGCGGGGCCACTGGCGAATCGCCGCCGGGCTCCTGTTC
NM_018656.2       ATGGCATTGCTGGTGGACCGAGTGCGGGGCCACTGGCGAATCGCCGCCGGGCTCCTGTTC
                  ************************************************************

HsCD00289079      AACCTGCTGGTGTCCATCTGCATTGTGTTCCTCAACAAATGGATTTATGTGTACCACGGC
NM_018656.2       AACCTGCTGGTGTCCATCTGCATTGTGTTCCTCAACAAATGGATTTATGTGTACCACGGC
                  ************************************************************

HsCD00289079      TTCCCCAACATGAGCCTGACCCTGGTGCACTTCGTGGTCACCTGGCTGGGCTTGTATATC
NM_018656.2       TTCCCCAACATGAGCCTGACCCTGGTGCACTTCGTGGTCACCTGGCTGGGCTTGTATATC
                  ************************************************************

HsCD00289079      TGCCAGAAGCTGGACATCTTTGCCCCCAAAAGTCTGCCGCCCTCCAGGCTCCTCCTCCTG
NM_018656.2       TGCCAGAAGCTGGACATCTTTGCCCCCAAAAGTCTGCCGCCCTCCAGGCTCCTCCTCCTG
                  ************************************************************

HsCD00289079      GCCCTCAGCTTCTGTGGCTTTGTGGTCTTCACTAACCTTTCTCTGCAGAACAACACCATA
NM_018656.2       GCCCTCAGCTTCTGTGGCTTTGTGGTCTTCACTAACCTTTCTCTGCAGAACAACACCATA
                  ************************************************************

HsCD00289079      GGCACCTATCAGCTGGCCAAGGCCATGACCACGCCGGTGATCATAGCCATCCAGACCTTC
NM_018656.2       GGCACCTATCAGCTGGCCAAGGCCATGACCACGCCGGTGATCATAGCCATCCAGACCTTC
                  ************************************************************

HsCD00289079      TGCTACCAGAAAACCTTCTCCACCAGAATCCAGCTCACGCTGATTCCTATAACTTTAGGT
NM_018656.2       TGCTACCAGAAAACCTTCTCCACCAGAATCCAGCTCACGCTGATTCCTATAACTTTAGGT
                  ************************************************************

HsCD00289079      GTAATCCTAAATTCTTATTACGATGTGAAGTTTAATTTCCTTGGAATGGTGTTTGCTGCT
NM_018656.2       GTAATCCTAAATTCTTATTACGATGTGAAGTTTAATTTCCTTGGAATGGTGTTTGCTGCT
                  ************************************************************

HsCD00289079      CTTGGTGTTTTAGTTACATCCCTTTATCAAGTGTGGGTAGGAGCCAAACAGCATGAATTA
NM_018656.2       CTTGGTGTTTTAGTTACATCCCTTTATCAAGTGTGGGTAGGAGCCAAACAGCATGAATTA
                  ************************************************************

HsCD00289079      CAAGTGAACTCAATGCAGCTGCTGTACTACCAGGCTCCGATGTCATCTGCCATGTTGCTG
NM_018656.2       CAAGTGAACTCAATGCAGCTGCTGTACTACCAGGCTCCGATGTCATCTGCCATGTTGCTG
                  ************************************************************

HsCD00289079      GTTGCTGTGCCCTTCTTTGAGCCAGTGTTTGGAGAAGGAGGAATATTTGGTCCCTGGTCA
NM_018656.2       GTTGCTGTGCCCTTCTTTGAGCCAGTGTTTGGAGAAGGAGGAATATTTGGTCCCTGGTCA
                  ************************************************************

HsCD00289079      GTTTCTGCTTTGCTTATGGTGCTGCTATCTGGAGTAATAGCTTTCATGGTGAACTTATCA
NM_018656.2       GTTTCTGCTTTGCTTATGGTGCTGCTATCTGGAGTAATAGCTTTCATGGTGAACTTATCA
                  ************************************************************

HsCD00289079      ATTTATTGGATCATTGGGAACACTTCACCTGTCACCTATAACATGTTCGGACACTTCAAG
NM_018656.2       ATTTATTGGATCATTGGGAACACTTCACCTGTCACCTATAACATGTTCGGACACTTCAAG
                  ************************************************************

HsCD00289079      TTCTGCATTACTTTATTCGGAGGATATGTTTTATTTAAGGATCCACTGTCCATTAATCAG
NM_018656.2       TTCTGCATTACTTTATTCGGAGGATATGTTTTATTTAAGGATCCACTGTCCATTAATCAG
                  ************************************************************

HsCD00289079      GCCCTTGGCATTTTATGTACATTATTTGGCATTCTCGCCTATACCCACTTTAAGCTCAGT
NM_018656.2       GCCCTTGGCATTTTATGTACATTATTTGGCATTCTCGCCTATACCCACTTTAAGCTCAGT
                  ************************************************************

HsCD00289079      GAACAGGAAGGAAGTAGGAGTAAACTGGCACAACGTCCTTAC
NM_018656.2       GAACAGGAAGGAAGTAGGAGTAAACTGGCACAACGTCCTTAA
                  *****************************************.