DNASU Plasmid | APLNR (Homo sapiens) in pJ2 (Cloning)

Explanation of Terms

Gene:

Gene Symbol: APLNR

Gene Name: apelin receptor

Original Clone ID:

ATCG3D_18

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pJ2

Format:

CLOSED

Source:

Accelerated Technologies Center for Gene to 3D Structure

Description:

G-protein coupled receptor

Comments:

Clone insert was fully verified. The insert sequence displayed below was provided by clone submitter. Codon Usage Engineered by DNA 2.0 for Expression in E. coli and BV. Supplied in the Vendor's Vector.

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

PSI
Accelerated Technologies Center for Gene to 3D Structure

HsCD00292919

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 1242nts

Open reading frame: 7 to 1248

Reference Sequence Alignment

GGATCCATGAAAACTATTATTGCTCTGTCCTACATCTTCTGTCTGGTCTTTGCCGATTAC
AAGGATGATGACGATATGGAAGAAGGTGGTGACTTCGATAACTATTACGGTGCTGACAAC
CAATCCGAATGCGAATATACTGACTGGAAGAGCTCTGGTGCTCTGATCCCAGCTATCTAT
ATGCTGGTATTCCTGCTGGGCACGACCGGTAACGGCCTGGTCCTGTGGACTGTGTTCCGC
TCCTCTCGCGAAAAACGTCGCTCCGCGGACATTTTCATTGCGTCTCTGGCAGTGGCTGAC
CTGACTTTCGTTGTAACCCTGCCACTGTGGGCGACCTACACCTACCGCGACTACGACTGG
CCTTTCGGCACGTTCTTCTGTAAGCTGTCTAGCTACCTGATCTTCGTGAACATGTATGCC
TCTGTTTTCTGCCTGACCGGCCTGTCCTTCGATCGTTATCTGGCGATCGTGCGTCCAGTC
GCTAACGCTCGTCTGCGTCTGCGCGTCTCCGGCGCAGTTGCAACCGCAGTGCTGTGGGTG
CTGGCTGCTCTGCTGGCTATGCCTGTCATGGTGCTGCGTACCACCGGCGACCTGGAAAAC
ACCACCAAAGTGCAGTGCTACATGGATTACTCTATGGTGGCGACCGTCAGCTCTGAGTGG
GCCTGGGAAGTTGGTCTGGGTGTCTCTTCTACTACTGTTGGTTTCGTGGTGCCGTTCACC
ATTATGCTGACGTGCTATTTCTTCATTGCTCAAACTATCGCTGGCCATTTCCGCAAGGAA
CGTATTGAGGGTCTGCGTAAGCGTCGCCGTCTGCTGTCCATCATCGTGGTCCTGGTCGTT
ACCTTTGCCCTGTGTTGGATGCCGTACCACCTGGTAAAAACGCTGTACATGCTGGGCAGC
CTGCTGCACTGGCCATGCGATTTCGATCTGTTCCTGATGAACATCTTTCCATACTGTACC
TGCATTTCTTATGTAAACAGCTGCCTGAATCCTTTTCTGTACGCCTTCTTTGACCCACGT
TTCCGTCAGGCCTGCACCAGCATGCTGTGTTGCGGCCAGAGCCGCTGCGCGGGCACTAGC
CACTCCTCTTCTGGCGAAAAGTCCGCTAGCTACAGCTCTGGCCACAGCCAAGGCCCTGGC
CCTAACATGGGCAAAGGCGGTGAACAAATGCACGAGAAATCCATTCCATATTCCCAGGAA
ACTCTGGTAGTGGACCACCACCATCACCATCACCATCACCATCACTGAAAGCTTCTCGAG

Protein sequence predicted based on nucleotide sequence

MKTIIALSYIFCLVFADYKDDDDMEEGGDFDNYYGADNQSECEYTDWKSSGALIPAIYML
VFLLGTTGNGLVLWTVFRSSREKRRSADIFIASLAVADLTFVVTLPLWATYTYRDYDWPF
GTFFCKLSSYLIFVNMYASVFCLTGLSFDRYLAIVRPVANARLRLRVSGAVATAVLWVLA
ALLAMPVMVLRTTGDLENTTKVQCYMDYSMVATVSSEWAWEVGLGVSSTTVGFVVPFTIM
LTCYFFIAQTIAGHFRKERIEGLRKRRRLLSIIVVLVVTFALCWMPYHLVKTLYMLGSLL
HWPCDFDLFLMNIFPYCTCISYVNSCLNPFLYAFFDPRFRQACTSMLCCGQSRCAGTSHS
SSGEKSASYSSGHSQGPGPNMGKGGEQMHEKSIPYSQETLVVDHHHHHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: kanamycin

Bacterial Selection Condition: kanamycin

Growth Condition: Growth in 30 ug/ml of kanamycin in LB at 37 degrees

Comments: None

Protein Expression Results:

ProteinExpressed: Not_Applicable

ProteinPurified: Not_Applicable

SolubleProtein: Not_Applicable

Find experimental protocols in ATCG3D_6

Recommended expression in:

Not Applicable

Vector Name:

pJ2

Synonyms:

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Cloning vector; kanamycin resistance in bacteria; restriction enzyme cloning.

Comments:

Supplied by DNA2.0

Size (bp):

2213

Parent Vector:

None

Properties:

TOPO Cloning

Author Name:

DNA2.0
Accelerated Technologies Center for Gene to 3D Structure

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

CTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGTACAAGATAAAATAATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGAGCCATATTCAACGGGAAACGTCGAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGCTTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCACTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGAAAAACAGCGTTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCACTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGCCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAATCAGAATTGGTTAATTGGTTGTAACACTGGCAGAGCATTACGCTGACTTGACGGGACGGCGCAAGCTCATGACCAAAATCCCTTAACGTGAGTTACGCGCGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGCCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGGCGAGAGTAGGGAACTGCCAGGCATCAAACTAAGCAGAAGGCCCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTCTGTGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACACATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGGCTCCCCCCCCCCACCCAGCTTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

pUC ori

Bacterial origin of replication

primer

M13 forward

M13 forward sequencing primer

2062

2077

primer

M13 reverse

M13 reverse sequencing primer

114

131

selectable marker

KanR

Kanamycin resistance gene

268

1050

Gene

Gene Symbol :

APLNR

Symbol Nomenclature :

APLNR

SYNONYM :

AGTRL1; APJ; APJR; HG11

Designation :

Full Nomenclature :

apelin receptor

GENEID :

187

HGNC :

339

MIM :

600052

Vega :

OTTHUMG00000167021

Target GenBank:

NP_005152

Reference Sequence Alignment

  

HsCD00292919      GGATCCATGAAAACTATTATTGCTCTGTCCTACATCTTCTGTCTGGTCTTTGCCGATTAC
NM_005161.4       ------------------------------------------------------------
                                                                              

HsCD00292919      AAGGATGATGACGATATGGAAGAAGGTGGTGACTTCGATAACTATTACGGTGCTGACAAC
NM_005161.4       ---------------ATGGAGGAAGGTGGTGATTTTGACAACTACTATGGGGCAGACAAC
                                 *****.*********** ** ** ***** ** ** **:******

HsCD00292919      CAATCCGAATGCGAATATACTGACTGGAAGAGCTCTGGTGCTCTGATCCCAGCTATCTAT
NM_005161.4       CAGTCTGAGTGTGAGTACACAGACTGGAAATCCTCGGGGGCCCTCATCCCTGCCATCTAC
                  **.** **.** **.** **:********.: *** ** ** ** *****:** ***** 

HsCD00292919      ATGCTGGTATTCCTGCTGGGCACGACCGGTAACGGCCTGGTCCTGTGGACTGTGTTCCGC
NM_005161.4       ATGTTGGTCTTCCTCCTGGGCACCACGGGCAACGGTCTGGTGCTCTGGACCGTGTTTCGG
                  *** ****.***** ******** ** ** ***** ***** ** ***** ***** ** 

HsCD00292919      TCCTCTCGCGAAAAACGTCGCTCCGCGGACATTTTCATTGCGTCTCTGGCAGTGGCTGAC
NM_005161.4       AGCAGCCGGGAGAAGAGGCGCTCAGCTGATATCTTCATTGCTAGCCTGGCGGTGGCTGAC
                  : *:  ** **.**..* *****.** ** ** ******** :  *****.*********

HsCD00292919      CTGACTTTCGTTGTAACCCTGCCACTGTGGGCGACCTACACCTACCGCGACTACGACTGG
NM_005161.4       CTGACCTTCGTGGTGACGCTGCCCCTGTGGGCTACCTACACGTACCGGGACTATGACTGG
                  ***** ***** **.** *****.******** ******** ***** ***** ******

HsCD00292919      CCTTTCGGCACGTTCTTCTGTAAGCTGTCTAGCTACCTGATCTTCGTGAACATGTATGCC
NM_005161.4       CCCTTTGGGACCTTCTTCTGCAAGCTCAGCAGCTACCTCATCTTCGTCAACATGTACGCC
                  ** ** ** ** ******** ***** :  ******** ******** ******** ***

HsCD00292919      TCTGTTTTCTGCCTGACCGGCCTGTCCTTCGATCGTTATCTGGCGATCGTGCGTCCAGTC
NM_005161.4       AGCGTCTTCTGCCTCACCGGCCTCAGCTTCGACCGCTACCTGGCCATCGTGAGGCCAGTG
                  :  ** ******** ******** : ****** ** ** ***** ******.* ***** 

HsCD00292919      GCTAACGCTCGTCTGCGTCTGCGCGTCTCCGGCGCAGTTGCAACCGCAGTGCTGTGGGTG
NM_005161.4       GCCAATGCTCGGCTGAGGCTGCGGGTCAGCGGGGCCGTGGCCACGGCAGTTCTTTGGGTG
                  ** ** ***** ***.* ***** ***: *** **.** **.** ***** ** ******

HsCD00292919      CTGGCTGCTCTGCTGGCTATGCCTGTCATGGTGCTGCGTACCACCGGCGACCTGGAAAAC
NM_005161.4       CTGGCCGCCCTCCTGGCCATGCCTGTCATGGTGTTACGCACCACCGGGGACTTGGAGAAC
                  ***** ** ** ***** *************** *.** ******** *** ****.***

HsCD00292919      ACCACCAAAGTGCAGTGCTACATGGATTACTCTATGGTGGCGACCGTCAGCTCTGAGTGG
NM_005161.4       ACCACTAAGGTGCAGTGCTACATGGACTACTCCATGGTGGCCACTGTGAGCTCAGAGTGG
                  ***** **.***************** ***** ******** ** ** *****:******

HsCD00292919      GCCTGGGAAGTTGGTCTGGGTGTCTCTTCTACTACTGTTGGTTTCGTGGTGCCGTTCACC
NM_005161.4       GCCTGGGAGGTGGGCCTTGGGGTCTCGTCCACCACCGTGGGCTTTGTGGTGCCCTTCACC
                  ********.** ** ** ** ***** ** ** ** ** ** ** ******** ******

HsCD00292919      ATTATGCTGACGTGCTATTTCTTCATTGCTCAAACTATCGCTGGCCATTTCCGCAAGGAA
NM_005161.4       ATCATGCTGACCTGTTACTTCTTCATCGCCCAAACCATCGCTGGCCACTTCCGCAAGGAA
                  ** ******** ** ** ******** ** ***** *********** ************

HsCD00292919      CGTATTGAGGGTCTGCGTAAGCGTCGCCGTCTGCTGTCCATCATCGTGGTCCTGGTCGTT
NM_005161.4       CGCATCGAGGGCCTGCGGAAGCGGCGCCGGCTGCTCAGCATCATCGTGGTGCTGGTGGTG
                  ** ** ***** ***** ***** ***** ***** : ************ ***** ** 

HsCD00292919      ACCTTTGCCCTGTGTTGGATGCCGTACCACCTGGTAAAAACGCTGTACATGCTGGGCAGC
NM_005161.4       ACCTTTGCCCTGTGCTGGATGCCCTACCACCTGGTGAAGACGCTGTACATGCTGGGCAGC
                  ************** ******** ***********.**.*********************

HsCD00292919      CTGCTGCACTGGCCATGCGATTTCGATCTGTTCCTGATGAACATCTTTCCATACTGTACC
NM_005161.4       CTGCTGCACTGGCCCTGTGACTTTGACCTCTTCCTCATGAACATCTTCCCCTACTGCACC
                  **************.** ** ** ** ** ***** *********** **.***** ***

HsCD00292919      TGCATTTCTTATGTAAACAGCTGCCTGAATCCTTTTCTGTACGCCTTCTTTGACCCACGT
NM_005161.4       TGCATCAGCTACGTCAACAGCTGCCTCAACCCCTTCCTCTATGCCTTTTTCGACCCCCGC
                  ***** :  ** **.*********** ** ** ** ** ** ***** ** *****.** 

HsCD00292919      TTCCGTCAGGCCTGCACCAGCATGCTGTGTTGCGGCCAGAGCCGCTGCGCGGGCACTAGC
NM_005161.4       TTCCGCCAGGCCTGCACCTCCATGCTCTGCTGTGGCCAGAGCAGGTGCGCAGGCACCTCC
                  ***** ************: ****** ** ** *********.* *****.***** : *

HsCD00292919      CACTCCTCTTCTGGCGAAAAGTCCGCTAGCTACAGCTCTGGCCACAGCCAAGGCCCTGGC
NM_005161.4       CACAGCAGCAGTGGGGAGAAGTCAGCCAGCTACTCTTCGGGGCACAGCCAGGGGCCCGGC
                  ***: *:  : *** **.*****.** ******:  ** ** ********.** ** ***

HsCD00292919      CCTAACATGGGCAAAGGCGGTGAACAAATGCACGAGAAATCCATTCCATATTCCCAGGAA
NM_005161.4       CCCAACATGGGCAAGGGTGGAGAACAGATGCACGAGAAATCCATCCCCTACAGCCAGGAG
                  ** ***********.** **:*****.***************** **.** : ******.

HsCD00292919      ACTCTGGTAGTGGACCACCACCATCACCATCACCATCACCATCACTGAAAGCTTCTCGAG
NM_005161.4       ACCCTTGTGGTTGACTAG------------------------------------------
                  ** ** **.** *** *

Pathways

Reactome :

Class A/1 (Rhodopsin-like receptors)

Reactome :

G alpha (i) signalling events

Reactome :

GPCR downstream signaling

Reactome :

GPCR ligand binding

Reactome :

Peptide ligand-binding receptors

Reactome :

Signal Transduction

Reactome :

Signaling by GPCR

WikiPathway :

GPCRs, Class A Rhodopsin-like

Protein

Structural Annotation Wiki :

TOPSAN

UniProt :

P35414

UniProt :

B2RDH3

UniProt :

B3KQN4

HPRD :

02503

Clone

Cloning Information :

485283

HIP Master Clone ID :

401840

Original Clone ID :

ATCG3D_18

Experimental

TargetTrack - Experimental data :

ATCG3D_6

Organism

Species Specific ID:

187

Genome

Chromosome :

Map Location :

11q12

Ensembl :

ENSG00000134817

Reagents

Labome :

APJ-antibody