DNASU Plasmid | CXCR5 (Homo sapiens) in pJ2 (Cloning)

Explanation of Terms

Gene:

Gene Symbol: CXCR5

Gene Name: chemokine (C-X-C motif) receptor 5

Original Clone ID:

ATCG3D_12

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pJ2

Format:

CLOSED

Source:

Accelerated Technologies Center for Gene to 3D Structure

Description:

G-protein coupled receptor

Comments:

Clone insert was fully verified. The insert sequence displayed below was provided by clone submitter. Codon Usage Engineered in Gene Composer for Expression in E. coli and BV. Supplied in the Vendor's Vector.

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

PSI
Accelerated Technologies Center for Gene to 3D Structure

HsCD00292923

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 1218nts

Open reading frame: 7 to 1224

Reference Sequence Alignment

GGATCCATGAAGACAATTATCGCCCTGAGCTATATCTTCTGTCTTGTGTTCGCTGATTAC
AAGGACGATGACGATATGAACTATCCGCTGACCCTGGAGATGGACCTTGAGAACCTCGAA
GACCTTTTCTGGGAACTGGATCGCCTGGACAACTACAACGACACAAGCCTCGTGGAGAAC
CACCTCTGCCCCGCCACCGAGGGGCCCCTGATGGCTTCCTTTAAGGCTGTGTTCGTTCCT
GTGGCTTACTCTCTTATTTTCCTCCTTGGCGTGATCGGTAATGTTCTGGTCCTGGTGATT
CTGGAGCGCCACCGCCAGACTCGTTCATCCACCGAGACTTTCCTGTTCCATCTCGCTGTG
GCCGACCTTCTCCTGGTGTTCATCCTGCCCTTCGCAGTCGCTGAAGGGTCCGTGGGCTGG
GTGCTTGGAACCTTTCTGTGCAAGACTGTGATCGCTCTGCATAAGGTTAACTTCTATTGT
TCTTCACTGCTCCTGGCCTGCATCGCAGTCGACCGTTATCTGGCTATCGTTCACGCCGTG
CACGCCTATCGCCACCGTCGTCTTCTGAGCATTCACATTACTTGCGGCACAATTTGGCTG
GTCGGCTTCCTTCTGGCACTGCCCGAGATCCTGTTCGCTAAAGTGAGCCAGGGCCACCAT
AACAATAGCCTTCCCCGCTGTACCTTCTCCCAGGAGAACCAGGCTGAAACCCACGCCTGG
TTTACAAGCCGCTTCCTGTACCACGTTGCCGGCTTTCTGCTGCCCATGCTTGTGATGGGT
TGGTGCTACGTCGGTGTCGTCCACCGCCTTCGTCAGGCTCAGCGTCGTCCTCAACGCCAG
AAGGCTGTTCGTGTCGCAATTCTTGTTACCTCCATCTTCTTCCTCTGTTGGTCACCTTAC
CATATCGTTATCTTCCTGGACACCCTTGCCCGTCTCAAGGCTGTGGACAACACCTGCAAG
CTGAACGGGAGTCTTCCCGTCGCAATCACCATGTGCGAGTTTCTCGGGCTGGCTCACTGC
TGTCTCAACCCGATGCTGTACACATTTGCTGGAGTCAAGTTCCGCTCCGACCTGAGCCGT
CTTCTGACAAAGCTGGGGTGTACGGGGCCTGCCTCCCTCTGCCAGCTCTTCCCTTCCTGG
CGTCGTAGCAGCCTCAGTGAGTCCGAAAATGCCACCAGCCTGACCACTTTCCACCACCAT
CACCATCACCATCACCATCACTGAAAGCTTCTCGAG

Protein sequence predicted based on nucleotide sequence

MKTIIALSYIFCLVFADYKDDDDMNYPLTLEMDLENLEDLFWELDRLDNYNDTSLVENHL
CPATEGPLMASFKAVFVPVAYSLIFLLGVIGNVLVLVILERHRQTRSSTETFLFHLAVAD
LLLVFILPFAVAEGSVGWVLGTFLCKTVIALHKVNFYCSSLLLACIAVDRYLAIVHAVHA
YRHRRLLSIHITCGTIWLVGFLLALPEILFAKVSQGHHNNSLPRCTFSQENQAETHAWFT
SRFLYHVAGFLLPMLVMGWCYVGVVHRLRQAQRRPQRQKAVRVAILVTSIFFLCWSPYHI
VIFLDTLARLKAVDNTCKLNGSLPVAITMCEFLGLAHCCLNPMLYTFAGVKFRSDLSRLL
TKLGCTGPASLCQLFPSWRRSSLSESENATSLTTFHHHHHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: kanamycin

Bacterial Selection Condition: 50ug/mL kanamycin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.

Protein Expression Results:

ProteinExpressed: Not_Applicable

ProteinPurified: Not_Applicable

SolubleProtein: Not_Applicable

Find experimental protocols in ATCG3D_12

Recommended expression in:

Not Applicable

Vector Name:

pJ2

Synonyms:

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Cloning vector; kanamycin resistance in bacteria; restriction enzyme cloning.

Comments:

Supplied by DNA2.0

Size (bp):

2213

Parent Vector:

None

Properties:

TOPO Cloning

Author Name:

DNA2.0
Accelerated Technologies Center for Gene to 3D Structure

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

CTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGTACAAGATAAAATAATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGAGCCATATTCAACGGGAAACGTCGAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGCTTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCACTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGAAAAACAGCGTTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCACTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGCCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAATCAGAATTGGTTAATTGGTTGTAACACTGGCAGAGCATTACGCTGACTTGACGGGACGGCGCAAGCTCATGACCAAAATCCCTTAACGTGAGTTACGCGCGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGCCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGGCGAGAGTAGGGAACTGCCAGGCATCAAACTAAGCAGAAGGCCCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTCTGTGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACACATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGGCTCCCCCCCCCCACCCAGCTTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

pUC ori

Bacterial origin of replication

primer

M13 forward

M13 forward sequencing primer

2062

2077

primer

M13 reverse

M13 reverse sequencing primer

114

131

selectable marker

KanR

Kanamycin resistance gene

268

1050

Gene

Gene Symbol :

CXCR5

Symbol Nomenclature :

CXCR5

SYNONYM :

BLR1; CD185; MDR15

Designation :

Full Nomenclature :

chemokine (C-X-C motif) receptor 5

GENEID :

643

HGNC :

1060

MIM :

601613

Target GenBank:

NP_001707

Reference Sequence Alignment

  

HsCD00292923      GGATCCATGAAGACAATTATCGCCCTGAGCTATATCTTCTGTCTTGTGTTCGCTGATTAC
NM_001716.4       ------------------------------------------------------------
                                                                              

HsCD00292923      AAGGACGATGACGATATGAACTATCCGCTGACCCTGGAGATGGACCTTGAGAACCTCGAA
NM_001716.4       ---------------ATGAACTACCCGCTAACGCTGGAAATGGACCTCGAGAACCTGGAG
                                 ******** *****.** *****.******** ******** **.

HsCD00292923      GACCTTTTCTGGGAACTGGATCGCCTGGACAACTACAACGACACAAGCCTCGTGGAGAAC
NM_001716.4       GACCTGTTCTGGGAACTGGACAGATTGGACAACTATAACGACACCTCCCTGGTGGAAAAT
                  ***** ************** .*. ********** ********.: *** *****.** 

HsCD00292923      CACCTCTGCCCCGCCACCGAGGGGCCCCTGATGGCTTCCTTTAAGGCTGTGTTCGTTCCT
NM_001716.4       CATCTCTGCCCTGCCACAGAGGGGCCCCTCATGGCCTCCTTCAAGGCCGTGTTCGTGCCC
                  ** ******** *****.*********** ***** ***** ***** ******** ** 

HsCD00292923      GTGGCTTACTCTCTTATTTTCCTCCTTGGCGTGATCGGTAATGTTCTGGTCCTGGTGATT
NM_001716.4       GTGGCCTACAGCCTCATCTTCCTCCTGGGCGTGATCGGCAACGTCCTGGTGCTGGTGATC
                  ***** ***:  ** ** ******** *********** ** ** ***** ******** 

HsCD00292923      CTGGAGCGCCACCGCCAGACTCGTTCATCCACCGAGACTTTCCTGTTCCATCTCGCTGTG
NM_001716.4       CTGGAGCGGCACCGGCAGACACGCAGTTCCACGGAGACCTTCCTGTTCCACCTGGCCGTG
                  ******** ***** *****:** : :***** ***** *********** ** ** ***

HsCD00292923      GCCGACCTTCTCCTGGTGTTCATCCTGCCCTTCGCAGTCGCTGAAGGGTCCGTGGGCTGG
NM_001716.4       GCCGACCTCCTGCTGGTCTTCATCTTGCCCTTTGCCGTGGCCGAGGGCTCTGTGGGCTGG
                  ******** ** ***** ****** ******* **.** ** **.** ** *********

HsCD00292923      GTGCTTGGAACCTTTCTGTGCAAGACTGTGATCGCTCTGCATAAGGTTAACTTCTATTGT
NM_001716.4       GTCCTGGGGACCTTCCTCTGCAAAACTGTGATTGCCCTGCACAAAGTCAACTTCTACTGC
                  ** ** **.***** ** *****.******** ** ***** **.** ******** ** 

HsCD00292923      TCTTCACTGCTCCTGGCCTGCATCGCAGTCGACCGTTATCTGGCTATCGTTCACGCCGTG
NM_001716.4       AGCAGCCTGCTCCTGGCCTGCATCGCCGTGGACCGCTACCTGGCCATTGTCCACGCCGTC
                  :  : .********************.** ***** ** ***** ** ** ******** 

HsCD00292923      CACGCCTATCGCCACCGTCGTCTTCTGAGCATTCACATTACTTGCGGCACAATTTGGCTG
NM_001716.4       CATGCCTACCGCCACCGCCGCCTCCTCTCCATCCACATCACCTGTGGGACCATCTGGCTG
                  ** ***** ******** ** ** ** : *** ***** ** ** ** **.** ******

HsCD00292923      GTCGGCTTCCTTCTGGCACTGCCCGAGATCCTGTTCGCTAAAGTGAGCCAGGGCCACCAT
NM_001716.4       GTGGGCTTCCTCCTTGCCTTGCCAGAGATTCTCTTCGCCAAAGTCAGCCAAGGCCATCAC
                  ** ******** ** **. ****.***** ** ***** ***** *****.***** ** 

HsCD00292923      AACAATAGCCTTCCCCGCTGTACCTTCTCCCAGGAGAACCAGGCTGAAACCCACGCCTGG
NM_001716.4       AACAACTCCCTGCCACGTTGCACCTTCTCCCAAGAGAACCAAGCAGAAACGCATGCCTGG
                  ***** : *** **.** ** ***********.********.**:***** ** ******

HsCD00292923      TTTACAAGCCGCTTCCTGTACCACGTTGCCGGCTTTCTGCTGCCCATGCTTGTGATGGGT
NM_001716.4       TTCACCTCCCGATTCCTCTACCATGTGGCGGGATTCCTGCTGCCCATGCTGGTGATGGGC
                  ** **.: ***.***** ***** ** ** **.** ************** ******** 

HsCD00292923      TGGTGCTACGTCGGTGTCGTCCACCGCCTTCGTCAGGCTCAGCGTCGTCCTCAACGCCAG
NM_001716.4       TGGTGCTACGTGGGGGTAGTGCACAGGTTGCGCCAGGCCCAGCGGCGCCCTCAGCGGCAG
                  *********** ** **.** ***.*  * ** ***** ***** ** *****.** ***

HsCD00292923      AAGGCTGTTCGTGTCGCAATTCTTGTTACCTCCATCTTCTTCCTCTGTTGGTCACCTTAC
NM_001716.4       AAGGCAGTCAGGGTGGCCATCCTGGTGACAAGCATCTTCTTCCTCTGCTGGTCACCCTAC
                  *****:** .* ** **.** ** ** **.: *************** ******** ***

HsCD00292923      CATATCGTTATCTTCCTGGACACCCTTGCCCGTCTCAAGGCTGTGGACAACACCTGCAAG
NM_001716.4       CACATCGTCATCTTCCTGGACACCCTGGCGAGGCTGAAGGCCGTGGACAATACCTGCAAG
                  ** ***** ***************** ** .* ** ***** ******** *********

HsCD00292923      CTGAACGGGAGTCTTCCCGTCGCAATCACCATGTGCGAGTTTCTCGGGCTGGCTCACTGC
NM_001716.4       CTGAATGGCTCTCTCCCCGTGGCCATCACCATGTGTGAGTTCCTGGGCCTGGCCCACTGC
                  ***** ** : *** ***** **.*********** ***** ** ** ***** ******

HsCD00292923      TGTCTCAACCCGATGCTGTACACATTTGCTGGAGTCAAGTTCCGCTCCGACCTGAGCCGT
NM_001716.4       TGCCTCAACCCCATGCTCTACACTTTCGCCGGCGTGAAGTTCCGCAGTGACCTGTCGCGG
                  ** ******** ***** *****:** ** **.** *********:  ******:  ** 

HsCD00292923      CTTCTGACAAAGCTGGGGTGTACGGGGCCTGCCTCCCTCTGCCAGCTCTTCCCTTCCTGG
NM_001716.4       CTCCTGACGAAGCTGGGCTGTACCGGCCCTGCCTCCCTGTGCCAGCTCTTCCCTAGCTGG
                  ** *****.******** ***** ** *********** ***************: ****

HsCD00292923      CGTCGTAGCAGCCTCAGTGAGTCCGAAAATGCCACCAGCCTGACCACTTTCCACCACCAT
NM_001716.4       CGCAGGAGCAGTCTCTCTGAGTCAGAGAATGCCACCTCTCTCACCACGTTCTAG------
                  ** .* ***** ***: ******.**.*********:  ** ***** *** *       

HsCD00292923      CACCATCACCATCACCATCACTGAAAGCTTCTCGAG
NM_001716.4       ------------------------------------

Pathways

Panther :

Inflammation mediated by chemokine and cytokine signaling pathway

Reactome :

Chemokine receptors bind chemokines

Reactome :

Class A/1 (Rhodopsin-like receptors)

Reactome :

G alpha (i) signalling events

Reactome :

GPCR downstream signaling

Reactome :

GPCR ligand binding

Reactome :

Peptide ligand-binding receptors

Reactome :

Signal Transduction

Reactome :

Signaling by GPCR

WikiPathway :

GPCRs, Class A Rhodopsin-like

WikiPathway :

Peptide GPCRs

Protein

Structural Annotation Wiki :

TOPSAN

UniProt :

Q2YD84

UniProt :

A0N0R2

UniProt :

A8K647

UniProt :

P32302

HPRD :

03367

Clone

Cloning Information :

485287

HIP Master Clone ID :

401844

Original Clone ID :

ATCG3D_12

Experimental

TargetTrack - Experimental data :

ATCG3D_12

Organism

Species Specific ID:

643

Genome

Chromosome :

Map Location :

11q23.3

Reagents

Labome :

CXCR5-antibody