Gene:
Gene Symbol:
GALR1
Gene Name:
galanin receptor 1
Original Clone ID:
ATCG3D_38
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJ2
Format:
CLOSED
Source:
GPCR Network
Description:
G-protein coupled receptor
Comments:
Clone insert was fully verified. The insert sequence displayed below was provided by clone submitter. ATCG3D-2 Cloned into our pET28a Expression Vector.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
PSI:Biology
Accelerated Technologies Center for Gene to 3D Structure
GPCR Network
PSI:Biology
Accelerated Technologies Center for Gene to 3D Structure
GPCR Network
HsCD00292927
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 1080nts
Open reading frame: 76 to 1155
GGATCCATGAAAACCATCATCGCCCTGAGCTATATCTTTTGCCTGGTTTTCGCTGACTAT
AAGGACGATGACGATATGGAACTGGCAGTCGGGAACCTGAGCGAGGGCAACGCCAGTTGT
CCTGAACCGCCAGCTCCTGAGCCCGGTCCTCTGTTTGGCATTGGCGTTGAAAACTTCGTG
ACCCTTGTCGTGTTCGGCCTGATCTTCGCCCTCGGCGTTCTCGGCAACTCACTTGTGATC
ACCGTTCTCGCTCGTTCTAAGCCAGGCAAACCACGTTCCACAACCAATCTGTTTATTCTC
AACCTTAGCATCGCCGACCTCGCATACCTGCTTTTCTGCATTCCGTTCCAGGCAACCGTT
TATGCTCTTCCAACATGGGTGCTGGGCGCTTTCATCTGTAAGTTTATTCATTACTTCTTT
ACAGTTTCTATGCTGGTCAGCATCTTCACGCTCGCCGCCATGTCTGTTGACCGCTACGTC
GCAATTGTTCATTCCCGCCGCTCATCCAGTCTTCGCGTGAGCCGTAACGCACTGCTGGGT
GTTGGGTGTATCTGGGCTCTGTCAATCGCTATGGCTAGCCCCGTGGCCTACCATCAAGGT
CTCTTCCATCCTCGTGCCTCAAACCAAACATTCTGTTGGGAGCAGTGGCCTGATCCTCGT
CATAAGAAGGCCTATGTTGTTTGTACATTCGTGTTCGGATACCTGCTCCCACTGCTCCTT
ATCTGCTTCTGCTATGCCAAAGTCCTGAACCACCTGCACAAGAAGCTCAAGAACATGAGC
AAGAAGTCCGAAGCAAGCAAGAAGAAAACTGCCCAGACAGTTCTCGTCGTGGTTGTGGTT
TTTGGAATTAGCTGGCTGCCACACCATATCATCCACCTGTGGGCCGAGTTCGGCGTGTTT
CCTCTCACCCCGGCCTCATTCCTTTTCCGTATCACGGCCCACTGTCTCGCTTACTCAAAT
TCATCCGTGAATCCTATTATCTACGCATTCCTTTCCGAAAATTTCCGCAAGGCCTACAAA
CAGGTGTTCAAGTGTCACATTCGTAAGGATTCACACCTCTCAGACACAAAGGAAAATAAG
TCCCGCATTGACACCCCTCCCAGCACCAATTGTACACACGTGCACCACCATCACCATCAC
CATCACCATCACTGAAAGCTTCTCGAG
Protein sequence predicted based on nucleotide sequence
MELAVGNLSEGNASCPEPPAPEPGPLFGIGVENFVTLVVFGLIFALGVLGNSLVITVLAR SKPGKPRSTTNLFILNLSIADLAYLLFCIPFQATVYALPTWVLGAFICKFIHYFFTVSML VSIFTLAAMSVDRYVAIVHSRRSSSLRVSRNALLGVGCIWALSIAMASPVAYHQGLFHPR ASNQTFCWEQWPDPRHKKAYVVCTFVFGYLLPLLLICFCYAKVLNHLHKKLKNMSKKSEA SKKKTAQTVLVVVVVFGISWLPHHIIHLWAEFGVFPLTPASFLFRITAHCLAYSNSSVNP IIYAFLSENFRKAYKQVFKCHIRKDSHLSDTKENKSRIDTPPSTNCTHVHHHHHHHHHH*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
kanamycin
Growth Condition:
Growth in 30 ug/ml of kanamycin in LB at 37 degrees
Comments:
None
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Not_Applicable
SolubleProtein: Not_Applicable
Find experimental protocols in ATCG3D_2
Find experimental protocols in GPCR-46
Recommended expression in:
Not Applicable
Vector Name:
pJ2
Synonyms:
''
Description:
Cloning vector; kanamycin resistance in bacteria; restriction enzyme cloning.
Comments:
Supplied by DNA2.0
Size (bp):
2213
Parent Vector:
None
Properties:
TOPO Cloning
Author Name:
DNA2.0
Accelerated Technologies Center for Gene to 3D Structure
Accelerated Technologies Center for Gene to 3D Structure
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
pUC ori
Bacterial origin of replication
0
0
primer
M13 forward
M13 forward sequencing primer
2062
2077
primer
M13 reverse
M13 reverse sequencing primer
114
131
selectable marker
KanR
Kanamycin resistance gene
268
1050
