Gene:
Gene Symbol:
GALR1
Gene Name:
galanin receptor 1
Original Clone ID:
GPCR-46
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJ2
Format:
CLOSED
Source:
GPCR Network
Description:
G-protein coupled receptor
Comments:
Clone insert was fully verified. The insert sequence displayed below was provided by clone submitter. Codon Usage Engineered by DNA2.0 for Expression in BV only. Supplied in the Vendor's Vector.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
PSI:Biology
Accelerated Technologies Center for Gene to 3D Structure
GPCR Network
PSI:Biology
Accelerated Technologies Center for Gene to 3D Structure
GPCR Network
HsCD00292949
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 1149nts
Open reading frame: 7 to 1155
GGATCCATGAAAACTATTATTGCGCTGTCCTATATATTCTGCTTGGTCTTCGCTGACTAT
AAAGACGACGATGACATGGAGCTCGCGGTAGGAAACTTGTCGGAAGGTAACGCTAGTTGT
CCAGAACCCCCTGCTCCTGAGCCCGGACCCCTCTTCGGAATCGGTGTCGAAAATTTTGTC
ACGTTGGTGGTGTTCGGACTCATCTTTGCCCTGGGAGTGCTTGGTAATTCTCTTGTGATC
ACTGTCTTGGCCCGTTCGAAACCCGGTAAGCCCAGGTCTACTACCAACCTGTTCATCCTC
AACCTGTCCATTGCGGACCTGGCATACCTTCTTTTCTGTATCCCTTTTCAGGCGACGGTG
TATGCCCTGCCGACGTGGGTTTTGGGTGCCTTCATCTGTAAATTCATCCACTACTTTTTC
ACAGTGTCCATGCTTGTCTCGATCTTCACCCTGGCCGCTATGTCAGTAGACCGTTATGTC
GCTATCGTGCACTCTCGTCGCAGTTCCTCACTCAGGGTGTCGAGGAATGCTCTCCTGGGT
GTTGGTTGTATCTGGGCTTTGTCGATTGCAATGGCAAGCCCAGTTGCCTACCATCAGGGA
CTCTTCCACCCCCGTGCCTCGAATCAAACCTTCTGTTGGGAACAATGGCCTGACCCTCGT
CACAAGAAAGCCTACGTTGTCTGCACCTTCGTCTTCGGTTACCTGTTGCCGCTGTTGCTG
ATATGTTTCTGCTATGCTAAGGTCTTGAACCACCTTCACAAGAAACTCAAGAACATGTCG
AAAAAGTCGGAGGCGAGTAAGAAGAAGACAGCACAGACTGTCCTCGTGGTCGTTGTCGTG
TTTGGAATTAGCTGGCTGCCCCACCATATCATTCACCTCTGGGCTGAATTCGGTGTCTTC
CCGCTCACGCCAGCTAGCTTCCTGTTCCGTATTACTGCTCACTGCTTGGCCTACTCCAAC
TCCTCCGTCAACCCAATCATCTATGCCTTCTTGAGCGAAAACTTCCGTAAAGCCTACAAG
CAGGTCTTTAAGTGTCACATCAGAAAGGATAGCCATTTGTCGGACACTAAGGAGAACAAG
AGTAGGATCGACACCCCTCCCTCTACAAACTGTACCCACGTGCACCACCATCACCATCAC
CATCACCATCACTGAAAGCTTCTCGAG
Protein sequence predicted based on nucleotide sequence
MKTIIALSYIFCLVFADYKDDDDMELAVGNLSEGNASCPEPPAPEPGPLFGIGVENFVTL VVFGLIFALGVLGNSLVITVLARSKPGKPRSTTNLFILNLSIADLAYLLFCIPFQATVYA LPTWVLGAFICKFIHYFFTVSMLVSIFTLAAMSVDRYVAIVHSRRSSSLRVSRNALLGVG CIWALSIAMASPVAYHQGLFHPRASNQTFCWEQWPDPRHKKAYVVCTFVFGYLLPLLLIC FCYAKVLNHLHKKLKNMSKKSEASKKKTAQTVLVVVVVFGISWLPHHIIHLWAEFGVFPL TPASFLFRITAHCLAYSNSSVNPIIYAFLSENFRKAYKQVFKCHIRKDSHLSDTKENKSR IDTPPSTNCTHVHHHHHHHHHH*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
kanamycin
Growth Condition:
Growth in 30 ug/ml of kanamycin in LB at 37 degrees
Comments:
None
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Not_Applicable
SolubleProtein: Not_Applicable
Find experimental protocols in ATCG3D_2
Find experimental protocols in GPCR-46
Recommended expression in:
Not Applicable
Vector Name:
pJ2
Synonyms:
''
Description:
Cloning vector; kanamycin resistance in bacteria; restriction enzyme cloning.
Comments:
Supplied by DNA2.0
Size (bp):
2213
Parent Vector:
None
Properties:
TOPO Cloning
Author Name:
DNA2.0
Accelerated Technologies Center for Gene to 3D Structure
Accelerated Technologies Center for Gene to 3D Structure
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
pUC ori
Bacterial origin of replication
0
0
primer
M13 forward
M13 forward sequencing primer
2062
2077
primer
M13 reverse
M13 reverse sequencing primer
114
131
selectable marker
KanR
Kanamycin resistance gene
268
1050
