Gene:
Gene Symbol:
ZNF335
Gene Name:
zinc finger protein 335
Original Clone ID:
FLH481199.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00294944
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGAGGAGAACGAGGTGGAGAGCAGCAGCGACGCGGCCCCTGGGCCTGGCCGGCCCGAG
GAGCCCTCTGAGAGCGGCCTGGGTGTGGGCACCTCAGAAGCCGTGTCCGCCGACAGCAGC
GACGCCGCGGCCGCCCCGGGGCAGGCAGAGGCCGATGACTCTGGCGTGGGGCAAAGCTCG
GACCGCGGCAGCCGTTCTCAGGAGGAGGTATCTGAGAGCAGCTCGAGCGCAGACCCCCTG
CCTAATAGCTACCTCCCTGATTCATCGTCTGTGTCTCATGGGCCAGTGGCAGGGGTGACA
GGCGGTCCCCCAGCACTTGTGCACTCTAGTGCACTCCCAGACCCCAACATGCTGGTGTCC
GACTGCACAGCTTCCTCCTCGGACCTGGGCTCGGCCATCGACAAGATCATCGAGTCCACC
ATCGGGCCCGACCTCATCCAGAACTGCATCACTGTGACCAGTGCTGAGGATGGCGGGGCC
GAGACCACACGGTACCTGATCCTACAGGGCCCAGATGATGGAGCCCCCATGACATCACCA
ATGTCCAGTTCCACCTTGGCCCACAGCCTAGCAGCCATTGAGGCCCTGGCAGATGGCCCC
ACATCCACATCCACATGCCTGGAGGCACAGGGTGGGCCCAGCTCCCCGGTGCAGCTGCCC
CCAGCCTCCGGTGCCGAAGAGCCGGACCTGCAGAGCCTGGAGGCCATGATGGAGGTGGTG
GTGGTGCAGCAGTTCAAATGCAAGATGTGCCAGTACCGGAGCAGCACCAAGGCCACACTG
CTGCGCCACATGCGGGAACGCCACTTCCGTCCAGTAGCAGCAGCCGCAGCAGCAGCTGGT
AAAAAAGGACGTCTACGGAAGTGGAGCACCTCCACCAAGAGCCAAGAGGAAGAGGGACCA
GAGGAGGAGGACGATGATGACATTGTAGACGCTGGAGCCATTGATGACCTGGAGGAGGAT
AGCGACTATAATCCAGCTGAGGATGAGCCCCGAGGCCGGCAGCTTCGGCTCCAGCGCCCC
ACCCCCAGTACCCCAAGGCCCCGAAGGAGACCTGGCCGGCCCCGGAAGCTGCCCCGCCTG
GAGATCTCAGACCTCCCAGATGGTGTGGAAGGAGAGCCTCTAGTGAGTTCCCAGAGTGGA
CAGAGCCCTCCAGAGCCACAGGATCCCGAGGCTCCCAGCTCCTCAGGCCCAGGACACCTG
GTGGCCATGGGCAAGGTGAGCAGGACCCCTGTGGAAGCTGGTGTGAGCCAGTCAGATGCA
GAGAACGCAGCCCCCTCCTGCCCGGATGAGCATGACACTCTGCCCCGGCGCCGAGGTCGA
CCTTCCAGGCGCTTCCTAGGCAAGAAATACCGCAAGTACTATTACAAGTCGCCCAAACCA
CTTTTGAGGCCCTTCCTGTGCCGCATCTGTGGTTCTCGCTTTCTGTCCCACGAGGACCTG
CGCTTCCACGTCAACTCCCATGAGGCTGGCGATCCCCAGCTCTTCAAGTGCCTGCAGTGC
AGCTATCGTTCCCGCCGCTGGTCCTCGCTCAAGGAGCACATGTTCAACCACGTGGGCAGC
AAGCCCTACAAGTGTGACGAGTGCAGCTACACCAGTGTCTACCGGAAGGACGTCATTCGG
CACGCCGCTGTGCACAGCCGGGACCGGAAGAAGAGGCCAGATCCGACTCCAAAGCTGAGC
TCTTTCCCCTGCCCTGTGTGTGGCCGTGTGTACCCCATGCAGAAAAGACTCACGCAGCAC
ATGAAGACGCACAGCACTGAGAAGCCCCACATGTGTGACAAGTGTGGAAAGTCCTTTAAG
AAGCGCTACACCTTCAAAATGCACCTGCTCACGCACATCCAGGCTGTTGCCAACCGCAGG
TTCAAGTGTGAGTTCTGTGAGTTTGTTTGTGAAGACAAGAAGGCACTGCTGAACCACCAG
TTGTCCCACGTCAGTGACAAGCCCTTCAAATGCAGCTTTTGTCCCTACCGCACCTTCCGA
GAGGACTTCTTGCTGTCCCATGTGGCTGTCAAGCACACAGGGGCCAAGCCCTTCGCCTGT
GAGTACTGCCACTTCAGCACACGGCACAAGAAGAACCTGCGCCTGCACGTACGGTGCCGA
CACGCAAGCAGCTTCGAGGAATGGGGGAGGCGCCACCCTGAGGAGCCCCCCTCCCGCCGT
CGCCCCTTCTTCTCTCTGCAGCAGATTGAGGAGCTGAAGCAGCAGCACAGTGCGGCCCCT
GGACCACCTCCCAGTTCCCCAGGACCTCCTGAGATACCCCCAGAGGCGACAACTTTCCAG
TCATCTGAGGCTCCCTCATTGCTCTGTTCTGACACCCTGGGCGGCGCCACCATCATCTAC
CAGCAAGGAGCTGAGGAGTCGACAGCGATGGCCACGCAGACAGCCTTGGATCTTCTGCTG
AACATGAGTGCTCAGCGGGAACTGGGGGGCACAGCCCTGCAGGTGGCTGTGGTGAAGTCG
GAAGATGTGGAAGCAGGGTTAGCATCCCCTGGTGGGCAGCCCTCCCCTGAAGGTGCCACT
CCACAGGTGGTCACCCTCCACGTGGCAGAGCCAGGGGGCGGTGCAGCAGCCGAGAGCCAG
CTAGGCCCTCCTGACCTACCGCAGATCACCCTGGCACCTGGTCCATTTGGTGGGACTGGC
TACAGTGTCATCACAGCACCCCCTATGGAGGAGGGAACATCAGCTCCTGGCACACCTTAC
AGCGAGGAGCCCGCAGGAGAGGCAGCCCAGGCTGTGGTTGTGAGTGACACCCTAAAAGAA
GCTGGCACCCACTACATCATGGCTACTGATGGTACCCAGTTGCACCACATTGAGCTCACC
GCAGATGGCTCCATCTCCTTCCCAAGTCCAGATGCTCTGGCCTCTGGTGCCAAATGGCCC
CTGCTGCAGTGTGGGGGACTGCCCAGAGACGGCCCTGAGCCCCCATCTCCAGCCAAGACC
CACTGCGTAGGGGACTCCCAGAGCTCTGCCTCCTCACCTCCTGCAACCAGCAAAGCCCTG
GGCCTGGCAGTGCCCCCGTCACCGCCATCTGCAGCCACTGCTGCATCAAAGAAGTTTTCC
TGCAAGATCTGTGCCGAGGCCTTCCCTGGCCGAGCTGAGATGGAGAGTCACAAGCGGGCC
CACGCTGGGCCTGGTGCCTTCAAGTGCCCCGACTGCCCCTTCAGTGCCCGCCAGTGGCCC
GAGGTCCGGGCGCACATGGCACAGCACTCAAGCCTACGGCCCCACCAGTGTAGCCAGTGC
AGCTTTGCCTCCAAGAACAAGAAGGACCTGCGTCGGCACATGCTGACTCACACAAAGGAG
AAGCCTTTTGCATGCCACCTCTGCGGGCAGCGTTTCAACCGTAACGGGCACCTCAAGTTC
CACATCCAGCGGCTGCACAGTCCTGATGGGAGGAAGTCAGGAACCCCTACAGCCCGGGCC
CCTACCCAGACCCCAACCCAGACCATCATCCTGAACAGTGATGACGAAACACTGGCCACC
CTGCACACTGCACTCCAGTCCAGTCACGGGGTCCTGGGCCCAGAGCGGCTACAGCAGGCA
CTGAGCCAGGAACACATCATCGTTGCCCAGGAACAGACAGTGACCAATCAGGAGGAAGCC
GCCTACATCCAAGAGATCACCACGGCAGATGGCCAGACCGTACAGCACCTGGTGACCTCC
GACAACCAGGTGCAGTATATCATCTCCCAGGATGGTGTCCAGCACCTGCTCCCCCAGGAA
TATGTTGTGGTCCCTGAAGGCCATCACATCCAGGTACAGGAGGGCCAGATCACACACATC
CAGTATGAACAAGGAGCCCCGTTCCTTCAGGAGTCCCAGATCCAGTATGTGCCTGTGTCC
CCAGGCCAGCAGCTTGTCACACAGGCTCAACTTGAGGCTGCAGCACACTCAGCTGTCACA
GCAGTGGCTGATGCTGCCATGGCCCAAGCCCAGGGCCTGTTTGGTACAGACGAGACAGTG
CCCGAACACATTCAACAGCTGCAGCACCAGGGCATCGAGTACGACGTCATCACCCTGGCC
GATGAC
Protein sequence predicted based on nucleotide sequence
MEENEVESSSDAAPGPGRPEEPSESGLGVGTSEAVSADSSDAAAAPGQAEADDSGVGQSS DRGSRSQEEVSESSSSADPLPNSYLPDSSSVSHGPVAGVTGGPPALVHSSALPDPNMLVS DCTASSSDLGSAIDKIIESTIGPDLIQNCITVTSAEDGGAETTRYLILQGPDDGAPMTSP MSSSTLAHSLAAIEALADGPTSTSTCLEAQGGPSSPVQLPPASGAEEPDLQSLEAMMEVV VVQQFKCKMCQYRSSTKATLLRHMRERHFRPVAAAAAAAGKKGRLRKWSTSTKSQEEEGP EEEDDDDIVDAGAIDDLEEDSDYNPAEDEPRGRQLRLQRPTPSTPRPRRRPGRPRKLPRL EISDLPDGVEGEPLVSSQSGQSPPEPQDPEAPSSSGPGHLVAMGKVSRTPVEAGVSQSDA ENAAPSCPDEHDTLPRRRGRPSRRFLGKKYRKYYYKSPKPLLRPFLCRICGSRFLSHEDL RFHVNSHEAGDPQLFKCLQCSYRSRRWSSLKEHMFNHVGSKPYKCDECSYTSVYRKDVIR HAAVHSRDRKKRPDPTPKLSSFPCPVCGRVYPMQKRLTQHMKTHSTEKPHMCDKCGKSFK KRYTFKMHLLTHIQAVANRRFKCEFCEFVCEDKKALLNHQLSHVSDKPFKCSFCPYRTFR EDFLLSHVAVKHTGAKPFACEYCHFSTRHKKNLRLHVRCRHASSFEEWGRRHPEEPPSRR RPFFSLQQIEELKQQHSAAPGPPPSSPGPPEIPPEATTFQSSEAPSLLCSDTLGGATIIY QQGAEESTAMATQTALDLLLNMSAQRELGGTALQVAVVKSEDVEAGLASPGGQPSPEGAT PQVVTLHVAEPGGGAAAESQLGPPDLPQITLAPGPFGGTGYSVITAPPMEEGTSAPGTPY SEEPAGEAAQAVVVSDTLKEAGTHYIMATDGTQLHHIELTADGSISFPSPDALASGAKWP LLQCGGLPRDGPEPPSPAKTHCVGDSQSSASSPPATSKALGLAVPPSPPSAATAASKKFS CKICAEAFPGRAEMESHKRAHAGPGAFKCPDCPFSARQWPEVRAHMAQHSSLRPHQCSQC SFASKNKKDLRRHMLTHTKEKPFACHLCGQRFNRNGHLKFHIQRLHSPDGRKSGTPTARA PTQTPTQTIILNSDDETLATLHTALQSSHGVLGPERLQQALSQEHIIVAQEQTVTNQEEA AYIQEITTADGQTVQHLVTSDNQVQYIISQDGVQHLLPQEYVVVPEGHHIQVQEGQITHI QYEQGAPFLQESQIQYVPVSPGQQLVTQAQLEAAAHSAVTAVADAAMAQAQGLFGTDETV PEHIQQLQHQGIEYDVITLADD
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
124
End on reference sequence
4152
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
