Gene:
Gene Symbol:
KALRN
Gene Name:
kalirin, RhoGEF kinase
Original Clone ID:
FLH481225.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00294970
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGACGGACCGCTTCTGGGACCAGTG
GTATCTCTGGTATCTCCGCTTGCTCCGGCTGCTGGATCGAGGGTCTTTTCGGAATGATGG
TTTGAAAGCTTCTGATGTCCTTCCTATCCTAAAGGAAAAGGTGGCCTTCGTGTCTGGGGG
TCGTGATAAGCGAGGCGGACCCATCCTGACCTTCCCTGCTCGCAGCAATCATGACAGAAT
AAGACAGGAAGACCTGCGGAAACTCGTGACGTATTTGGCCAGCGTGCCAAGTGAGGACGT
GTGCAAACGTGGCTTCACTGTCATCATCGACATGCGGGGCTCCAAGTGGGACCTCATCAA
GCCCCTCCTCAAAACGCTGCAGGAAGCCTTTCCAGCTGAGATCCATGTGGCCCTCATCAT
TAAACCCGACAACTTCTGGCAGAAACAGAAGACCAACTTTGGCAGCTCCAAATTCATCTT
TGAGACGAGCATGGTATCTGTGGAGGGCCTCACAAAGCTGGTGGACCCCTCCCAGCTGAC
GGAGGAGTTTGATGGCTCCCTGGACTACAACCATGAGGAGTGGATCGAACTGCGGCTCTC
CCTGGAGGAGTTCTTCAACAGCGCCGTGCACCTGCTCTCGCGCCTCGAGGACCTCCAGGA
GATGCTAGCCCGGAAGGAGTTTCCTGTGGATGTGGAGGGCTCTCGGCGGCTCATTGACGA
ACACACACAGCTCAAGAAAAAGGTGCTGAAGGCCCCTGTGGAGGAGCTGGACCGGGAGGG
GCAGCGGCTGCTGCAGTGCATCCGCTGCAGCGACGGCTTCTCAGGACGCAACTGCATCCC
GGGCAGTGCTGACTTCCAGAGCCTGGTGCCCAAGATCACCAGTCTCCTGGACAAGCTGCA
CTCCACCCGGCAGCACCTGCACCAGATGTGGCATGTGCGCAAGCTCAAGCTGGACCAGTG
CTTTCAGCTGCGGCTCTTCGAGCAGGATGCTGAGAAGATGTTTGACTGGATAAGCCACAA
CAAGGAGTTATTCCTCCAGAGCCACACGGAGATCGGAGTCAGCTACCAGTACGCCCTTGA
CCTCCAGACGCAGCACAATCACTTTGCCATGAACTCCATGAATGCCTATGTCAACATCAA
CCGCATCATGTCCGTGGCTTCCCGCCTCTCTGAGGCCGGTCATTATGCCTCACAACAAAT
CAAGCAGATCTCCACCCAGCTGGACCAGGAGTGGAAGAGCTTCGCTGCTGCCCTGGATGA
ACGCAGCACCATCCTCGCCATGTCTGCTGTGTTCCACCAGAAGGCTGAGCAGTTCCTGTC
GGGAGTGGATGCCTGGTGCAAGATGTGCAGTGAAGGTGGTCTGCCATCCGAGATGCAAGA
CCTAGAGCTGGCAATCCACCACCACCAGACCTTGTATGAGCAGGTGACCCAAGCCTACAC
AGAGGTCAGCCAGGATGGCAAAGCACTACTTGATGTGCTGCAGCGGCCCCTGAGCCCTGG
GAACTCCGAATCCCTCACGGCCACAGCCAACTACTCCAAGGCAGTGCACCAGGTGCTGGA
CGTGGTGCATGAGGTGTTACATCACCAGCGACGGCTGGAGAGCATCTGGCAGCACCGCAA
GGTGCGGCTCCACCAGCGGCTGCAGCTCTGCGTCTTCCAGCAGGATGTACAGCAGGTGTT
GGACTGGATTGAAAACCATGGTGAGGCCTTTCTCAGCAAACACACTGGAGTTGGGAAGTC
CCTACATCGAGCCCGGGCCCTGCAGAAGAGGCATGATGACTTTGAAGAGGTGGCTCAGAA
TACGTACACCAATGCGGACAAGCTCCTAGAAGCAGCAGAGCAGTTGGCTCAGACGGGGGA
ATGTGACCCCGAGGAGATCTACAAGGCAGCTCGACACCTGGAGGTGCGCATCCAAGACTT
CGTGCGCAGGGTGGAGCAGCGGAAGCTTCTCCTGGACATGTCTGTTTCCTTCCACACACA
CACCAAAGAGTTGTGGACATGGATGGAAGACCTTCAGAAGGAGATGTTGGAGGATGTCTG
TGCAGATTCTGTGGATGCAGTCCAGGAACTGATCAAGCAGTTCCAGCAGCAGCAGACCGC
CACTCTAGATGCCACACTCAATGTCATCAAGGAAGGCGAAGACCTTATCCAGCAGCTCAG
GTCAGCGCCTCCCTCCCTCGGGGAGCCCAGCGAGGCCAGGGACTCGGCTGTGTCCAACAA
CAAAACACCCCACAGCAGCTCCATCAGCCACATCGAGTCGGTCCTGCAGCAGCTTGATGA
TGCCCAGGTGCAGATGGAGGAGCTGTTCCACGAGCGGAAGATCAAGCTGGACATCTTCCT
GCAACTGCGCATCTTTGAGCAGTACACCATCGAGGTGACAGCAGAGCTAGACGCCTGGAA
TGAAGACTTGCTTCGGCAGATGAATGACTTCAACACAGAGGACCTAACCCTGGCAGAACA
GCGGCTGCAGCGCCACACAGAACGGAAGCTAGCCATGAACAACATGACCTTTGAGGTTAT
CCAGCAGGGACAGGATCTGCACCAGTACATCACGGAGGTCCAGGCATCAGGAATTGAGTT
GATCTGTGAAAAAGACATTGATCTGGCAGCCCAGGTGCAAGAGTTATTGGAATTTCTCCA
TGAGAAGCAGCATGAATTGGAGCTCAATGCAGAGCAGACTCATAAGCGGCTAGAGCAGTG
CCTCCAATTACGTCACCTCCAGGCTGAAGTCAAACAGGTTCTGGGATGGATCCGCAATGG
AGAGTCAATGCTCAACGCCAGCCTGGTCAATGCCAGCTCTTTGTCGGAAGCAGAGCAGCT
GCAGCGGGAGCACGAGCAGTTCCAACTGGCCATCGAGTCCCTCTTTCATGCCACTTCCTT
GCAGAAGACGCACCAGAGTGCCCTGCAGGTACAGCAGAAAGCCGAGGTGCTGCTCCAGGC
CGGCCACTACGATGCCGATGCCATCCGGGAATGTGCTGAGAAGGTGGCCCTCCACTGGCA
GCAGCTCATGCTGAAGATGGAAGACCGGCTAAAATTGGTCAATGCCTCTGTGGCCTTTTA
CAAAACTTCTGAACAGGTGTGTAGTGTCCTGGAGAGCTTAGAGCAAGAATACCGGAGAGA
TGAGGACTGGTGTGGTGGACGAGATAAGCTGGGGCCAGCAGCAGAGATCGACCATGTCAT
TCCCCTCATCAGCAAACATTTGGAACAAAAGGAGGCCTTTCTTAAGGCCTGCACCCTGGC
TCGGCGGAATGCTGAGGTGTTTCTCAAGTACATCCACAGGAACAACGTCAGCATGCCCAG
TGTCGCCAGCCACACTCGGGGACCCGAGCAACAAGTGAAAGCCATCCTGAGTGAGCTCCT
GCAGAGGGAGAATCGCGTGCTGCATTTCTGGACCTTGAAGAAGCGGCGGTTAGACCAATG
CCAGCAATATGTGGTGTTCGAGCGCAGCGCTAAGCAGGCGCTTGACTGGATCCAAGAAAC
AGGTGAATTTTACCTCTCAACACATACCTCCACTGGAGAGACCACAGAGGAGACTCAGGA
ACTGCTGAAAGAATATGGGGAATTCAGGGTGCCTGCCAAGCAAACAAAGGAGAAGGTGAA
GCTTCTGATTCAGCTGGCCGATAGCTTTGTGGAAAAAGGCCACATTCATGCCACGGAGAT
AAGGAAATGGGTGACCACGGTGGACAAGCACTACAGAGATTTCTCCCTGAGGATGGGAAA
GTACCGATACTCACTGGAGAAAGCCCTAGGAGTCAACACAGAGGATAATAAGGACCTGGA
GCTGGATATTATCCCAGCAAGCCTTTCGGATCGGGAGGTCAAGCTGCGGGACGCCAACCA
CGAAGTCAATGAAGAGAAGCGGAAGTCAGCCCGGAAGAAAGAATTTATTATGGCTGAACT
ACTCCAGACAGAGAAGGCTTATGTAAGGGATTTGCATGAGTGCTTAGAGACCTACCTGTG
GGAAATGACCAGTGGTGTGGAGGAGATCCCCCCTGGGATCCTCAATAAAGAGCATATCAT
CTTTGGCAACATCCAAGAGATCTACGATTTCCATAACAACATCTTCCTCAAAGAGCTGGA
GAAGTACGAGCAACTGCCTGAGGATGTGGGACACTGCTTTGTTACCTGGGCAGACAAATT
TCAGATGTATGTCACCTACTGTAAAAACAAGCCTGATTCCAACCAGCTTATCCTGGAGCA
TGCGGGCACCTTCTTTGATGAGATACAACAGCGGCATGGTCTGGCCAACTCCATCTCTTC
CTACCTAATTAAGCCTGTCCAAAGGATCACCAAATATCAACTGCTCCTGAAGGAACTTTT
AACTTGCTGTGAAGAAGGGAAAGGGGAGCTCAAGGATGGCCTGGAGGTGATGCTCAGTGT
CCCAAAGAAAGCCAATGATGCCATGCATGTCAGCATGCTGGAAGGGTTCGACGAGAACCT
GGATGTGCAGGGGGAGTTGATTCTCCAGGATGCCTTTCAAGTGTGGGACCCGAAGTCGCT
GATCCGGAAGGGGCGGGAGCGGCACTTGTTCCTCTTTGAGATCTCCTTGGTTTTTAGCAA
GGAGATCAAAGATTCTTCAGGACACACGAAATATGTTTACAAGAACAAGCTACTGACCTC
AGAGCTGGGTGTGACCGAGCACGTGGAGGGCGATCCCTGCAAATTCGCCTTGTGGTCTGG
GCGCACCCCATCCTCAGACAATAAAACAGTGCTGAAAGCCTCCAACATTGAAACCAAGCA
GGAGTGGATCAAGAACATTCGAGAAGTGATTCAAGAAAGGATCATTCACCTGAAAGGAGC
TTTAAAGGAGCCACTTCAGCTCCCCAAAACACCAGCCAAACAGAGGAACAATAGTAAGAG
GGATGGAGTGGAGGATATTGACAGCCAGGGGGATGGGAGCAGCCAACCAGACACCATCTC
CATTGCTTCTAGGACCTCTCAGAACACAGTGGACAGTGACAAGGATGGCAACCTTGTTCC
TCGGTGGCACCTGGGACCTGGAGATCCTTTCTCCACTTACGTTTAGGGCCTCATGGGCCC
AGCTTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MTDRFWDQWYLWYLRLLRLLDRGSFRNDGLKASDVLPILKEKVAFVSGGRDKRGGPILTF PARSNHDRIRQEDLRKLVTYLASVPSEDVCKRGFTVIIDMRGSKWDLIKPLLKTLQEAFP AEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVSVEGLTKLVDPSQLTEEFDGSLDYNH EEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDVEGSRRLIDEHTQLKKKVLKA PVEELDREGQRLLQCIRCSDGFSGRNCIPGSADFQSLVPKITSLLDKLHSTRQHLHQMWH VRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQYALDLQTQHNHFAMN SMNAYVNINRIMSVASRLSEAGHYASQQIKQISTQLDQEWKSFAAALDERSTILAMSAVF HQKAEQFLSGVDAWCKMCSEGGLPSEMQDLELAIHHHQTLYEQVTQAYTEVSQDGKALLD VLQRPLSPGNSESLTATANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCV FQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEA AEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDL QKEMLEDVCADSVDAVQELIKQFQQQQTATLDATLNVIKEGEDLIQQLRSAPPSLGEPSE ARDSAVSNNKTPHSSSISHIESVLQQLDDAQVQMEELFHERKIKLDIFLQLRIFEQYTIE VTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTERKLAMNNMTFEVIQQGQDLHQYIT EVQASGIELICEKDIDLAAQVQELLEFLHEKQHELELNAEQTHKRLEQCLQLRHLQAEVK QVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHATSLQKTHQSALQVQ QKAEVLLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLE SLEQEYRRDEDWCGGRDKLGPAAEIDHVIPLISKHLEQKEAFLKACTLARRNAEVFLKYI HRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSAK QALDWIQETGEFYLSTHTSTGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVE KGHIHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVNTEDNKDLELDIIPASLSDR EVKLRDANHEVNEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEEIPP GILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKP DSNQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRITKYQLLLKELLTCCEEGKGELK DGLEVMLSVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFL FEISLVFSKEIKDSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPSSDNKTVL KASNIETKQEWIKNIREVIQERIIHLKGALKEPLQLPKTPAKQRNNSKRDGVEDIDSQGD GSSQPDTISIASRTSQNTVDSDKDGNLVPRWHLGPGDPFSTYV*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
101
End on reference sequence
5092
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
