Gene:
Gene Symbol:
Pcdh9
Gene Name:
protocadherin 9
Original Clone ID:
FLH481277.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00295022
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3714nts
Open reading frame: 35 to 3748
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGGACCTGAGGGATTTTTACCTGTT
GGCTGCTCTGATTGCCTGTTTAAGGCTGGATTCCGCAATAGCTCAAGAACTTATTTACAC
TATTAGAGAGGAATTGCCTGAAAATGTGCCCATAGGAAATATACCAAAGGATCTGAATAT
TTCTCACATCAATGCTGCCACGGGGACCAGTGCCAGCCTTGTCTACAGACTGGTTTCAAA
AGCTGGGGATGCCCCTCTGGTGAAAGTATCGAGTAGCACTGGGGAGATTTTCACAACCTC
CAACAGAATAGACAGAGAGAAACTCTGTGCTGGGGCTTCATACGCTGAGGAGAACGAGTG
CTTCTTTGAACTTGAAGTAGTGATCCTCCCCAATGACTTTTTCAGGCTGATCAAAATCAA
GATCATTGTCAAGGATACCAATGATAATGCCCCCATGTTTCCGTCTCCTGTCATCAATAT
TTCCATCCCAGAGAACACTTTGATCAACAGCCGCTTTCCAATCCCATCAGCCACAGACCC
TGACACGGGCTTCAATGGGGTACAGCATTATGAATTACTAAATGGACAGAGTGTTTTTGG
ACTGGATATCGTGGAAACTCCGGAGGGAGAAAAGTGGCCGCAATTGATTGTTCAGCAGAA
CTTGGATAGAGAACAAAAAGATACCTATGTGATGAAAATCAAAGTGGAGGATGGGGGCAC
GCCACAGAAATCGAGCACAGCTATTCTCCAGGTCACAGTAAGTGATGTCAATGACAACAG
GCCAGTGTTTAAAGAGGGTCAAGTAGAAGTGCATATTCCAGAGAATGCTCCTGTGGGGAC
CTCTGTCATTCAGCTCCATGCCACTGATGCAGACATAGGCAGTAATGCTGAAATCCGGTA
TATTTTTGGTGCCCAGGTCGCCCCTGCAACCAAAAGACTCTTTGCTTTAAATAATACAAC
TGGGCTGATAACAGTTCAGAGGTCTTTAGATAGGGAAGAGACAGCCATTCACAAAGTGAC
AGTGCTGGCTAGTGACGGCAGCTCCACTCCTGCCAGAGCCACCGTGACCATCAATGTCAC
CGATGTAAATGATAACCCTCCAAACATAGACCTCAGGTACATTATAAGTCCCATCAATGG
CACAGTGTATCTCTCTGAGAAAGACCCTGTCAATACAAAGATTGCCCTCATTACAGTTTC
AGATAAGGACACAGATGTGAATGGCAAAGTGATCTGTTTTATTGAAAGAGAGGTCCCATT
TCATTTGAAGGCAGTGTATGACAACCAGTATCTGCTAGAGACCTCTTCTCTGCTGGACTA
TGAGGGCACCAAAGAATTCAGCTTTAAAATCGTTGCCTCTGATTCGGGGAAGCCCAGTTT
GAATCAGACTGCCCTGGTAAGGGTTAAGCTCGAGGATGAAAATGACAACCCACCAATTTT
CAACCAGCCTGTAATTGAGCTGTCAGTTTCTGAAAACAACCGACGTGGGTTATACTTAAC
AACTATTAGTGCTACAGATGAAGACAGTGGAAAAAATGCAGACATTGTTTATCAGCTTGG
ACCGAATGCCTCCTTCTTTGATCTGGACAGAAAGACAGGAGTTTTGACAGCCTCCAGAGT
CTTTAACAGGGAGGAACAAGAGCGGTTCATTTTTACAGTAACTGCCAGGGACAATGGGAC
CCCTCCCCTCCAGAGCCAAGCAGCAGTGATAGTTACTGTTCTGGATGAGAATGACAATAG
CCCCAAGTTTACTCATAATCACTTTCAGTTTTTTGTGTCTGAGAATCTGCCAAAGTATAG
TACTGTGGGGGTAATCACAGTGACAGACGAAGACGCTGGAGAGAATAAAGCTGTGACTCT
TTCCATTCTAAATGACAATGAAAATTTTGTGTTGGATCCCTATTCAGGAGTCATAAAGTC
CAATGTCTCCTTTGATAGAGAGCAGCAGAGTTCCTACACGTTTGACGTCAAAGCCACCGA
CGGGGGACAGCCACCACGGTCCTCTACTGCAAAAGTAACTATCAATGTCATGGATGTCAA
TGACAATAGTCCAGTTGTCATTTCTCCACCATCCAACACCTCCTTTAAGTTGGTGCCCCT
CTCAGCCATTCCTGGTTCTGTGGTTGCTGAAGTGTTTGCGGTGGATATTGACACCGGAAT
GAATGCAGAACTCAAGTATACCATAGTGAGCGGGAACAACAAGGGATTATTTCGGATTGA
TCCAGTAACAGGCAATATCACCTTAGAAGAGAAGCCAGCACCTACTGATGTGGGCCTGCA
CCGTCTTGTGGTCAACATAAGTGACCTGGGGTACCCTAAGGCTTTGCACACTCTCGTGCT
GGTATTTCTTTATGTGAACGACACTGCTGGAAACACCTCATATATATATGACTTGATCCG
TAGGACTATGGAGACCCCACTGGACCGGAACATAGGAGATAGTGGTCAACCTTATCAGAA
CGAGGACTATCTCACCATCATGATTGCCATCGTTGCTGGGGCCATGGTGGTCATTGTTGT
GATCTTTGTCACGGTGCTGGTGCGCTGTCGCCATGCGTCCAGGTTCAAGGCCGCTCAGAG
GAGCAAACAAGGGGCTGAATGGATGTCCCCAAACCAGGAGAATAAACAAAATAAGAAAAA
GAAACGGAAGAAAAGAAAGTCGCCCAAGAGTTCTCTTTTGAACTTTGTTACTATTGAAGA
GTCCAAACCCGATGATGCCGTTCATGAACCCATCAATGGGACCATAAGCCTGCCTGCCGA
GCTGGAGGAACAGGGTATAGGAAGATTTGACTGGGGCCCAGCCCCTCCAACCACCTTCAA
GCCTAACAGCCCTGACCTGGCCAAGCACTACAAGTCGGCCTCTCCTCAGCCCGCTTTTCA
TCTCAAACCTGACACTCCAGTTTCTGTGAAAAAGCACCACGTCATTCAAGAGCTCCCGTT
GGACAACACCTTTGTTGGGGGCTGCGACACCCTTTCCAAACGCTCCTCCACTAGTTCAGA
TCACTTCAGTGCCTCAGAGTGCAGCTCCCAAGGAGGCTTCAAGACAAAGGGCCCCTTACA
CACCAGACAGTGTAACTCATACAGCAAAAATGACAATATTCCTGTCACACATCAGAAATG
TCCCGGCTCTACCAGTTTCCACATTCGAGAGAATGAAGAAAGTCATTCTGAGTCTCAGCG
CCGTGTTACCTTTCACCTTCCGGATGGCTCCCAGGAAAGCTGCAGTGACAGTGGTCTGGG
AGACCACGAGCCGGTGGGTAGTGGAACCCTGATCTCACACCCTCTTCCTCTGGTTCAGCC
ACAGGACGAATTCTACGACCAGGCCTCTCCGGACAAGAGGACCGAAGCAGATGGCAACTC
GGATCCCAATTCTGATGGACCTCTGGGTCCCCGAGGTTTAGCTGAAGCTACAGAGATGTG
CACTCAAGAGTGTCTGGTTTTGGGTCACTCTGATAATTGCTGGATGCCTCCTGGCTTGGG
TCCATACCAACACCCTAAATCTCCTCTGTCAACCTTTGCACCCCAGAAAGAATGGATCAA
GAAAGACAAGCTTGTGAATGGGCACACCCTGACAAGAGCGTGGAAAGAAGACACCAACAG
GAACCAATTCAATGACCGTAAGCAGTATGGCTCCAATGAAGGCCATTTCAACAATGGCGG
CCACATGGCGGACATTCCTCTGGCAAATCTGAAGTCCTATAAGCAAGCAGGAGGCACGAT
TGAGAGTCCTAAGGAGCACCAACTTTAGGGCCTCATGGGCCCAGCTTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MDLRDFYLLAALIACLRLDSAIAQELIYTIREELPENVPIGNIPKDLNISHINAATGTSA SLVYRLVSKAGDAPLVKVSSSTGEIFTTSNRIDREKLCAGASYAEENECFFELEVVILPN DFFRLIKIKIIVKDTNDNAPMFPSPVINISIPENTLINSRFPIPSATDPDTGFNGVQHYE LLNGQSVFGLDIVETPEGEKWPQLIVQQNLDREQKDTYVMKIKVEDGGTPQKSSTAILQV TVSDVNDNRPVFKEGQVEVHIPENAPVGTSVIQLHATDADIGSNAEIRYIFGAQVAPATK RLFALNNTTGLITVQRSLDREETAIHKVTVLASDGSSTPARATVTINVTDVNDNPPNIDL RYIISPINGTVYLSEKDPVNTKIALITVSDKDTDVNGKVICFIEREVPFHLKAVYDNQYL LETSSLLDYEGTKEFSFKIVASDSGKPSLNQTALVRVKLEDENDNPPIFNQPVIELSVSE NNRRGLYLTTISATDEDSGKNADIVYQLGPNASFFDLDRKTGVLTASRVFNREEQERFIF TVTARDNGTPPLQSQAAVIVTVLDENDNSPKFTHNHFQFFVSENLPKYSTVGVITVTDED AGENKAVTLSILNDNENFVLDPYSGVIKSNVSFDREQQSSYTFDVKATDGGQPPRSSTAK VTINVMDVNDNSPVVISPPSNTSFKLVPLSAIPGSVVAEVFAVDIDTGMNAELKYTIVSG NNKGLFRIDPVTGNITLEEKPAPTDVGLHRLVVNISDLGYPKALHTLVLVFLYVNDTAGN TSYIYDLIRRTMETPLDRNIGDSGQPYQNEDYLTIMIAIVAGAMVVIVVIFVTVLVRCRH ASRFKAAQRSKQGAEWMSPNQENKQNKKKKRKKRKSPKSSLLNFVTIEESKPDDAVHEPI NGTISLPAELEEQGIGRFDWGPAPPTTFKPNSPDLAKHYKSASPQPAFHLKPDTPVSVKK HHVIQELPLDNTFVGGCDTLSKRSSTSSDHFSASECSSQGGFKTKGPLHTRQCNSYSKND NIPVTHQKCPGSTSFHIRENEESHSESQRRVTFHLPDGSQESCSDSGLGDHEPVGSGTLI SHPLPLVQPQDEFYDQASPDKRTEADGNSDPNSDGPLGPRGLAEATEMCTQECLVLGHSD NCWMPPGLGPYQHPKSPLSTFAPQKEWIKKDKLVNGHTLTRAWKEDTNRNQFNDRKQYGS NEGHFNNGGHMADIPLANLKSYKQAGGTIESPKEHQL*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
3714
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
