Gene:
Gene Symbol:
Sp1
Gene Name:
trans-acting transcription factor 1
Original Clone ID:
FLH481284.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00295029
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2346nts
Open reading frame: 35 to 2380
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGAGCGACCAAGATCACTCCATGGA
TGAAGTGACAGCTGTGGTGAAGATTGAAAAAGATGTTGGTGGCAATAATGGGGGTAGCGG
CAATGGTGGCGGTGCCGCCTTTTCTCAGACTCGAAGCAGCAGCACAGGCAGTAGCAGCAG
CAGTGGTGGCGGAGGAGGGCAGGAATCCCAGCCATCTCCTTTGGCTCTGCTGGCAGCAAC
CTGCAGCAGAATTGAGTCACCCAATGAGAACAGCAACAACTCCCAGGGTCCGAGTCAGTC
AGGGGGCACAGGTGAACTTGACCTCACAGCCGCACAACTTTCACAGGGTGCCAATGGCTG
GCAGATCATCTCTTCCTCCTCTGGGGCTACCCCTACCTCAAAGGAACAGAGTGGCAACAG
TACCAATGGCAGCGAGTCTTCCAAGAACCGCACAGTCTCTGGTGGGCAGTATGTTGTGGC
TGCTACCCCCAACTTACAGAACCAGCAAGTTCTGACAGGTCTCCCTGGAGTAATGCCTAA
TATTCAGTATCAAGTAATCCCACAGTTCCAGACTGTTGATGGGCAGCAGCTGCAGTTTGC
TGCCACTGGGGCCCAAGTGCAGCAGGATGGTTCTGGTCAAATACAGATCATACCAGGTGC
AAACCAACAGATCATCCCAAATAGAGGAAGTGGGGGCAACATTATTGCTGCTATGCCAAA
TCTACTCCAGCAGGCTGTCCCCCTTCAAGGCCTTGCTAATAATGTGCTCTCAGGACAGAC
TCAGTATGTGACCAATGTACCAGTGGCCCTGAATGGGAACATCACCTTGCTACCTGTCAA
CAGCGTTTCTGCAGCTACCCTGACTCCCAGCTCTCAGGCAGGCACTATCAGCAGCTCTGG
ATCCCAGGAGAGCAGCTCACAGCCTGTCACCTCAGGGACTGCCATCAGTTCTGCCAGCTT
GGTGTCATCACAAGCTAGTTCCAGCTCCTTTTTTACCAATGCCAATAGTTATTCAACAAC
TACTACCACCAGCAACATGGGAATTATGAACTTTACCAGCAGTGGCTCATCAGGGACTAG
TTCTCAAGGCCAGACGCCCCAGAGGGTTGGTGGGCTACAAGGGTCTGATTCTCTGAACAT
CCAGCAGAACCAGACATCAGGAGGCTCGCTGCAAGGAAGTCAGCAGAAAGAGGGAGAGCA
AAGTCAGCAGACACAGCAACAACAAATCCTTATTCAGCCTCAGCTAGTTCAAGGAGGACA
AGCTCTTCAGGCCCTTCAAGCAGCACCATTGTCCGGACAGACCTTCACAACTCAAGCTAT
TTCCCAGGAAACCCTTCAGAACCTCCAGCTTCAGGCTGTTCAAAACTCTGGTCCCATCAT
CATTCGGACACCAACCGTGGGGCCCAATGGACAGGTCAGTTGGCAGACCCTTCAGCTGCA
GAATCTTCAAGTTCAGAACCCACAAGCCCAGACAATCACCTTGGCCCCTATGCAGGGTGT
TTCCTTGGGGCAGACTAGCAGCAGTAATACCACCCTAACACCCATTGCCTCAGCTGCCTC
CATTCCTGCTGGCACAGTCACTGTGAATGCTGCTCAACTCTCCTCCATGCCAGGCCTCCA
GACCATTAACCTCAGTGCATTGGGTACTTCAGGGATCCAGGTGCACCAGCTTCCAGGCCT
GCCTTTGGCTATAGCAAACACCCCAGGTGATCATGGAACTCAACTTGGTCTTCATGGATC
TGGTGGTGATGGGATACATGATGAGACAGCAGGTGGAGAAGGAGAGAATAGCTCTGATCT
CCAACCCCAAGCTGGACGCAGGACTCGTCGGGAAGCATGTACATGCCCCTATTGCAAAGA
CAGTGAGGGAAGAGCCTCAGGAGATCCTGGCAAAAAGAAACAGCACATTTGTCACATCCA
AGGATGCGGCAAAGTATATGGCAAGACCTCACATCTCCGAGCACACTTGCGCTGGCATAC
AGGGGAGAGGCCATTCATGTGTAATTGGTCATATTGTGGGAAGCGCTTTACACGTTCGGA
CGAGCTTCAGAGACATAAACGTACACATACAGGAGAGAAGAAATTTGCCTGCCCTGAGTG
CCCTAAGCGTTTCATGAGGAGTGATCACCTGTCAAAGCATATCAAGACTCACCAGAACAA
GAAGGGAGGCCCAGGTGTAGCCCTGAGTGTGGGCACATTGCCCCTGGACAGTGGGGCAGG
TTCAGAAGGCACTGCCACTCCTTCAGCCCTTATTACCACCAATATGGTAGCCATGGAGGC
CATCTGTCCAGAGGGTATTGCCCGTCTTGCCAACAGTGGCATCAACGTCATGCAGGTGAC
AGAGCTGCAGTCCATTAATATCAGTGGCAATGGTTTCTAGGGCCTCATGGGCCCAGCTTT
CTTGTAC
Protein sequence predicted based on nucleotide sequence
MSDQDHSMDEVTAVVKIEKDVGGNNGGSGNGGGAAFSQTRSSSTGSSSSSGGGGGQESQP SPLALLAATCSRIESPNENSNNSQGPSQSGGTGELDLTAAQLSQGANGWQIISSSSGATP TSKEQSGNSTNGSESSKNRTVSGGQYVVAATPNLQNQQVLTGLPGVMPNIQYQVIPQFQT VDGQQLQFAATGAQVQQDGSGQIQIIPGANQQIIPNRGSGGNIIAAMPNLLQQAVPLQGL ANNVLSGQTQYVTNVPVALNGNITLLPVNSVSAATLTPSSQAGTISSSGSQESSSQPVTS GTAISSASLVSSQASSSSFFTNANSYSTTTTTSNMGIMNFTSSGSSGTSSQGQTPQRVGG LQGSDSLNIQQNQTSGGSLQGSQQKEGEQSQQTQQQQILIQPQLVQGGQALQALQAAPLS GQTFTTQAISQETLQNLQLQAVQNSGPIIIRTPTVGPNGQVSWQTLQLQNLQVQNPQAQT ITLAPMQGVSLGQTSSSNTTLTPIASAASIPAGTVTVNAAQLSSMPGLQTINLSALGTSG IQVHQLPGLPLAIANTPGDHGTQLGLHGSGGDGIHDETAGGEGENSSDLQPQAGRRTRRE ACTCPYCKDSEGRASGDPGKKKQHICHIQGCGKVYGKTSHLRAHLRWHTGERPFMCNWSY CGKRFTRSDELQRHKRTHTGEKKFACPECPKRFMRSDHLSKHIKTHQNKKGGPGVALSVG TLPLDSGAGSEGTATPSALITTNMVAMEAICPEGIARLANSGINVMQVTELQSINISGNG F*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
101
End on reference sequence
2446
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
