Gene:
Gene Symbol:
Bnc1
Gene Name:
basonuclin 1
Original Clone ID:
FLH481285.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00295030
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2973nts
Open reading frame: 35 to 3007
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGCGGCGGTCGCCGAGCCGGGGCGG
ACGCGGAGCGGCCCGGGCAGGGGACGCGCGCCGGGAAGGCCGGCTCCGCAGCGGCTGCAG
GATGGCTGAGGCTATCGGCTGTACTCTGAACTGCAGTTGCCAATGTTTCAAACCTGGGAA
GATAAACCACCGTCAGTGTGAACAGTGCAGGCACGGATGGGTGGCTCATGCTCTGAGTAA
GCTGAGGATCCCCCCTGTGTATCCAACAAGCCAAGTGGAAATTGTCCAGTCCAATGTGGT
GTTCGATATTAGCAGCCTCATGCTCTATGGAACCCAGGCTATCCCTGTTCGCCTGAAAAT
CCTATTGGACCGGCTCTTCAGTGTGCTGAAGCAAGATGAGGTTCTACAGATCCTCCATGC
CCTGGACTGGACGCTTCAGGATTACATCCGTGGATATGTGTTACAGGATGCCTCTGGGAA
GGTGCTGGATCACTGGAGCATCATGACCAGTGAGGAGGAAGTGGCTACCTTGCAGCAGTT
CCTTCGCTTTGGAGAGACCAAGTCCATAGTTGAACTCATGGCAATCCAAGAGAAAGAAGA
GCAGTCTGTCATCGTACCGCCCACCACGGCCAATGTAGACATCAGGGCTTTCATCGAGAG
CTGCGGCCACAGGAGTGCCAGCCTCCCTACTCCTGTGGACAAAGGAAGCCCTGGCGGCAT
GCACCCCTTTGAGAACCTTATAAGCAACATGACGTTCATGTTGCCTTTCCAGTTCTTCAA
CCCTCTGCCTCCTGCTCTGATAGGGTCGCTGCCTGAACAGTACATGCTGGAGCAGGGGCA
GGACCAAAGCCAGGAACCCAAACAGGAGCTCCATGGGCCCTTCTCGGACAGCAGCTTCTT
AACTTCCACCCCATTTCAGGTTGAAAAAGAACAGTGCCTCAACTGTCCAGAAACTGTCCC
CCAAAAGGAAGACAGTGCTCACTTAAGCGACTCCAGCTCATATAGCATTGCCAGTAAGCT
TGAAAGGACACAGCTGTCCCCTGAGGCCAAGGTGAAGCCTGAGAGGAACAGCCTCAGTGC
TAAGAAGGGCCGGGTGTTCTGCACGGCGTGCGAGAAGACATTCTATGACAAAGGCACTCT
GAAGATCCACTACAACGCCGTCCACCTAAAGATCAAGCACAAGTGCACGATCGAAGGGTG
TAACATGGTGTTCAGCTCCCTGAGGAGCCGGAACCGACACAGCGCCAACCCCAACCCTCG
CCTGCACATGCCAATGAACAGAAACAACAGGGACAAAGATCTCAGGAATAGCCTGAACCT
GGCAAGCTCTGAGACCTACAAGCGCCCAGGTTTCACAGTGGTGTCTCCAGACTGTGGGCC
CCTCCCTGGCTACACTGGTTCCGTGGAGGACTCCAAAGGCCAACCTGCTTTCTCAAGCAT
TGGGCAAAATGGTGTGCTTTTCCCTAATCTAAAGACTGTCCAGCCAGTCCTTCCTTTCTA
CCGCAGTCCAGCCACTCCCGCGGAGCTGGCCAACACACCCGGCATGCTGCCTTCCCTCCC
TCTGTTGTCCTCTTCGATCCCAGAACAGCTGGTTTCCACAGACATGCCATTCGATGCTCT
TCCCAAGAAGAAATCCCGCAAGTCCAGTATGCCTATCAAAATAGAGAAAGAAGCTGTGGA
AATAGCTGAGGAGAAGAGACACAGTCTCAGCTCAGACGATGAGGTGCCTCTGCAGGTAGT
CAGTGAAGACGAGCCAGAGGACAGCAGTCCTAGGTCAGACAGAGTACCTGAGGAGCAGCA
TACACAGCTAAGCTTAGAGGAGCCTCTTCCACAAGGAGAAAGAGCCTGCCATCTTGAATC
TGTGATTGAGTCACATGGAGCTCTCAGCAGAACCCTTGAGCAGACCACGCTCACTGAGAG
GGAGGCTGAACAGAAGGTAGCACTGAGTTCAGTGATGCCAAGAGAGGTGGAGGATGGTGG
CCACGAGCGCCATTTCACAGCTGGCCTGGTACCCCAAATCCCTTTTCCTGACTACATGGA
GTTGCAGCAGCGCCTGCTTGCTGGGGGACTCTTCGGTGCTTTGTCCAACAGGGGGATGGC
TTTTCCTTTTCTGGAAGAGTCTAAAGAGCTGGAGCACCTGGGTGAGCATGCACTAGTGAG
GCAGAAGGAAGAAGCTTGCTTCCAGTGTGACATCTGCAAGAAGACCTTTAAAAACGCTTG
CAGCATGAAGACGCATGAGAAGAACACACACGCCCGAGAGACACACGCGTGCACAGTGGA
GGGCTGCGGGGCTGCTTTCCCTTCCCGTAGGAGCAGAGACAGACACAGCTCGAACCTAAG
CCTCCACCAGAAAGTACTGAATGAAGAAGCATTGGAGACTAGTGAGGACCATTTTCGTGC
AGCTTACCTTCTGCAAGATGTGGCCAAGGAGGCCTATCAGGATGTGGCTTTCACGCCACA
AGCATCACAGACATCTGTCATCTTCAAGGGAACAAGTGGCATGGGCAGTCTGGTTTACCC
AATATCTCAAGTTCACAGTGCCAGCCTGGAGAGCTACAACTCTGGCCCCCCTAGCGAGGG
CACCATCCTGGATTTGAGCACTACCTCAAGTATGAAGTCAGAGAGCAGCAGTCATTCCTC
CTGGGACTCTGATGGCGTGAGTGAGGAAGGCACTGCCCTTATGGAGGACAGTGATGGCAA
CTGTGAAGGACAGAGCCTTGTCTCTGGGGAAGATGAATACCCTCTCTGTGTCCTAATGGA
GAAGGCAGACCAGAGCCTTGCTAGCCTGCCCTCTGGTTTGCCAATAACCTGTCATCTCTG
CCAAAAGATATACAGTAACAAAGGGACCTTTAGAGCCCATTACAAAACCGTGCACCTCCG
CCAGCTCCACAAGTGCAAAGTGCCAGGCTGCAACACGATGTTCTCATCTGTTCGCAGTAG
AAACAGACACAGTCAGAATCCCAACCTGCACAAGAGCCTGGCTTCATCTCCAAGCCACCT
CCAGTAGGGCCTCATGGGCCCAGCTTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MRRSPSRGGRGAARAGDARREGRLRSGCRMAEAIGCTLNCSCQCFKPGKINHRQCEQCRH GWVAHALSKLRIPPVYPTSQVEIVQSNVVFDISSLMLYGTQAIPVRLKILLDRLFSVLKQ DEVLQILHALDWTLQDYIRGYVLQDASGKVLDHWSIMTSEEEVATLQQFLRFGETKSIVE LMAIQEKEEQSVIVPPTTANVDIRAFIESCGHRSASLPTPVDKGSPGGMHPFENLISNMT FMLPFQFFNPLPPALIGSLPEQYMLEQGQDQSQEPKQELHGPFSDSSFLTSTPFQVEKEQ CLNCPETVPQKEDSAHLSDSSSYSIASKLERTQLSPEAKVKPERNSLSAKKGRVFCTACE KTFYDKGTLKIHYNAVHLKIKHKCTIEGCNMVFSSLRSRNRHSANPNPRLHMPMNRNNRD KDLRNSLNLASSETYKRPGFTVVSPDCGPLPGYTGSVEDSKGQPAFSSIGQNGVLFPNLK TVQPVLPFYRSPATPAELANTPGMLPSLPLLSSSIPEQLVSTDMPFDALPKKKSRKSSMP IKIEKEAVEIAEEKRHSLSSDDEVPLQVVSEDEPEDSSPRSDRVPEEQHTQLSLEEPLPQ GERACHLESVIESHGALSRTLEQTTLTEREAEQKVALSSVMPREVEDGGHERHFTAGLVP QIPFPDYMELQQRLLAGGLFGALSNRGMAFPFLEESKELEHLGEHALVRQKEEACFQCDI CKKTFKNACSMKTHEKNTHARETHACTVEGCGAAFPSRRSRDRHSSNLSLHQKVLNEEAL ETSEDHFRAAYLLQDVAKEAYQDVAFTPQASQTSVIFKGTSGMGSLVYPISQVHSASLES YNSGPPSEGTILDLSTTSSMKSESSSHSSWDSDGVSEEGTALMEDSDGNCEGQSLVSGED EYPLCVLMEKADQSLASLPSGLPITCHLCQKIYSNKGTFRAHYKTVHLRQLHKCKVPGCN TMFSSVRSRNRHSQNPNLHKSLASSPSHLQ*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
10
End on reference sequence
2982
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
