Gene:
Gene Symbol:
Dsg4
Gene Name:
desmoglein 4
Original Clone ID:
FLH481305.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00295050
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3126nts
Open reading frame: 35 to 3160
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGGACTGGCTTCTCTTCAGAAATAT
TTGCCTTCTGATCTTATTTATGGTGGTACTGGGAGTAAACAGTGAATTTATCGTTGAGGT
AAAAGAATTAGACATTGAAAATGGCACAACAACCTGGCAGACTGTCAGAAGACAAAAGCG
GGAGTGGATCAAGTTTGCGGCAGCCTGTCGGGAGGGCGAGGACAACTCCAAGAGGAATCC
AATTGCCAGAATTAGATCAGACTGTGAAGTAAGCCAGAGGATAACCTATCGAATCTCTGG
AGCGGGGATTGATCGGCCACCTTACGGAGTGTTCACCATCAACCCTCGGACTGGAGAAAT
CAATATTACCTCAGTGGTGGACAGAGAGATAACCCCACTGTTCCTGATCCATTGCCGAGC
TCTCAACTCCCGAGGAGAAGACTTGGAGAGACCACTGGAGCTCAGAGTGAAAGTCATGGA
TGTGAACGACAACCCCCCAGTCTTCACTCAGAATGTGTACACAGCCAACATTGAAGAGAA
CAGCGACGCCAATGCACTGGTGGTAAAGTTAAGTGCCACAGATGCAGATGAAGACAATCA
CTTGAATTCTAAAATTGCATACAAGATCATCTCCCAGGAGCCTGCTGGTGCACCCATGTT
CATGGTGAACAGGTACACTGGAGAAGTCCGCACGATGTCCAATTTCCTTGACAGAGAGCA
ACACAGTATGTACAATCTACTGGTGAGGGGCTCAGATCGGGATGGAGCCACAGATGGGCT
GTCCTCTGAATGTGACTGCAGAATCAAGATTTTGGATGTCAATGATAATTTCCCCATCTT
GGAGAAGACCTCATACTCAGCAAGTATTGAAGAAAATTGTTTGAGCTCAGAACTGATACG
ACTACAAGCAATCGATCTGGATGAAGAAGGCACTGATAACTGGTTGGCACAATACTCCAT
CCTCTCTGGCAATGATGGGAACTGGTTTGAAATCCAAACAGATCCAAAAACCAATGAAGG
CATTTTGAAAGTGGTCAAGATGCTGGATTATGAACAAGAACCTAACATTTACCTCAGCAT
TGGAGTTAGAAACCAAGCTGAGTTTCACCACTCAGTAGCATCTCAATTCCAAATGCACTC
TACCCCTGTGAGAATCCAGGTTGTTAACGTGCGGGAAGGACCGACCTTTAGCCCAAGTTC
CATGACATTTAGTCTACGAGGAGGAATGAGAGGAGCCTCCTTAATGAACTATGTGCTTGG
CACATACACAGCCATAGATATGGACACAGGAAACCCTGCAACCAATGTCAGGTATGTCAT
AGGGCATGATGCAGGCAGCTGGTTAAAAGTTGATTCAAGGACTGGTGAGATACAGTTTTC
TAGGGAATTTGACATGAAGTCAAAATATATTACGGATGGGATATATGCAGCACAGATCCT
GGCTATAGATGATGGCTCTGGGAGAACAGCTACAGGAACCATATGCATTGAGATTCCTGA
TGCCAACGATTACTGTCCCGTCATTTATGCTGAAAGCAGGAGTGTCTGCACCCATGCTTC
GTCTGTCAGGATCTATGTCAACGATCATTCTTTCGGGTCTCCCTTTACCTTTTGTGTAGT
TGATGAGTCACCAGACATAGCTAATATATGGGATATCAGGTCCATCAATGGAACCTCTGC
AATCCTTATGACTGAGCAGACTTTGTCTCCAGGACCCTACCAAATCCCCATCCTCGTGAA
GGACAGTCACAACCGAGCATGTGAATTGCCACAGACAGTGCTGTTAGATGCCTGCTTTTG
TGATGACCACCATGTGTGCTTGCACTCCAGTACCACGGGCATCTACACTGGAGACATCAC
CTGGGTGACTGATGACATGTATGGGACTGTCACTGATGATGGAGTTAGGCAGTCAAACGT
CGGACTTGGCCCAGCAGGGATTGGCATGATTATTTTGGGGCTCTTGCTACTGCTCTTGTC
ACCCCTTTTACTGCTCATGTGTTGCTGCAAGCGAAGACAGCCAGAAGGCCTGGGGACAAG
GTTTGCCCCTGTGCCTGAGGGAGGCGAAGGAGTGATGCAGCCCTGGAGAATAGAGGGGGC
TCATCCAGAAGACAGGGATGTATCAAATATTTGTGTACCCATGACAGCCTCTAATACCCA
AGATCGCATTGATTCATCTGAAATCTACACCAATACTTACGCTGGTGGAGGAACAGTGGA
AGGGGGCGTGTCAGGAGTGGAGCTCAATACAGGTGTTGGGACAGCCACAGGCATGGTGGC
TGCAGGAGCAACAGGGACCCTAAGGAAGAGGAGTTCAACTATAGGGACTCTTCGGGAATA
CCAAGACACTGGCATGAACATGGCCTTCCTGGACAGCTACTTCTCCGAGAAAGCGTATGC
ATATGCAGATGAAGATGAAGGCCGACCAGCCAATGACTGCCTGCTCATTTATGATCACGA
AGGAGCTGGGTCACCCGTGGGCTCCATTGGCTGCTGCAGTTGGATTGTGGATGACTTAGA
CGAAAGCTACATAGAGACTTTGGATCCAAAGTTCAGGACTCTAGCTGAAATCTGCTTGGA
CACAGAGATTGAACCCTTCCCTTCACACCAGGCCTGTATACCAATCAGCACTGACCTCCC
TCTGCTGGGGCCTAATTACTTTGTCAATGAGTCTTCAGGAATGACTCTCTCAGAGGCTGA
ATTCCAAGCAGAAATGGCAGCAGCCTCTGAACCCATGATTCATGGGGACATCATAGTGAC
TGAGACCTACACTACTTCTGACCCATGTGTCCAACCCACAACGATTGTCTTTGACTCTCA
GATTCCACCAAACGTAGTCGTGACCGAAACCGTAATGGCACCTGTCTACGATGTTCAAGG
GAACATTTGTGTGCCTGCTGAGATAGCCAACACACACAATGTATACTATGCTGAGAGAGT
GGTGGCTAGCCCTGGTATACCTGACATGGGCAACAGTAACATTAGTGATGCATGTATAGG
ACCAGTGATGAGCGGCGGTATTTTGGTAGGGCCAGAAATTCAAGTGACACAAATGATGAG
CCCAGACATTCACATAAGCCAAACCACAGGCTCCACCTCCCCAATGACGTCTCAACACCG
AGTAACCAGATACAGCAATATGCACTACTCCCGACAGTAGGGCCTCATGGGCCCAGCTTT
CTTGTAC
Protein sequence predicted based on nucleotide sequence
MDWLLFRNICLLILFMVVLGVNSEFIVEVKELDIENGTTTWQTVRRQKREWIKFAAACRE GEDNSKRNPIARIRSDCEVSQRITYRISGAGIDRPPYGVFTINPRTGEINITSVVDREIT PLFLIHCRALNSRGEDLERPLELRVKVMDVNDNPPVFTQNVYTANIEENSDANALVVKLS ATDADEDNHLNSKIAYKIISQEPAGAPMFMVNRYTGEVRTMSNFLDREQHSMYNLLVRGS DRDGATDGLSSECDCRIKILDVNDNFPILEKTSYSASIEENCLSSELIRLQAIDLDEEGT DNWLAQYSILSGNDGNWFEIQTDPKTNEGILKVVKMLDYEQEPNIYLSIGVRNQAEFHHS VASQFQMHSTPVRIQVVNVREGPTFSPSSMTFSLRGGMRGASLMNYVLGTYTAIDMDTGN PATNVRYVIGHDAGSWLKVDSRTGEIQFSREFDMKSKYITDGIYAAQILAIDDGSGRTAT GTICIEIPDANDYCPVIYAESRSVCTHASSVRIYVNDHSFGSPFTFCVVDESPDIANIWD IRSINGTSAILMTEQTLSPGPYQIPILVKDSHNRACELPQTVLLDACFCDDHHVCLHSST TGIYTGDITWVTDDMYGTVTDDGVRQSNVGLGPAGIGMIILGLLLLLLSPLLLLMCCCKR RQPEGLGTRFAPVPEGGEGVMQPWRIEGAHPEDRDVSNICVPMTASNTQDRIDSSEIYTN TYAGGGTVEGGVSGVELNTGVGTATGMVAAGATGTLRKRSSTIGTLREYQDTGMNMAFLD SYFSEKAYAYADEDEGRPANDCLLIYDHEGAGSPVGSIGCCSWIVDDLDESYIETLDPKF RTLAEICLDTEIEPFPSHQACIPISTDLPLLGPNYFVNESSGMTLSEAEFQAEMAAASEP MIHGDIIVTETYTTSDPCVQPTTIVFDSQIPPNVVVTETVMAPVYDVQGNICVPAEIANT HNVYYAERVVASPGIPDMGNSNISDACIGPVMSGGILVGPEIQVTQMMSPDIHISQTTGS TSPMTSQHRVTRYSNMHYSRQ*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
135
End on reference sequence
3260
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
