Gene:
Gene Symbol:
Aff2
Gene Name:
AF4/FMR2 family, member 2
Original Clone ID:
FLH481315.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00295060
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3819nts
Open reading frame: 35 to 3853
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGGATCTATTCGACTTTTTCAGGGA
TTGGGACTTGGAGCAGCAGTGTCACTATGAACAAGACCGTAGTGCACTTAAAAAAAGGGA
ATGGGAACGAAGGAATCAAGAAGTTCAGCAAGAAGAAGATCTCTTTTCTTCAGGCTTTGA
TCTTTTTGGGGAGCCCTACAAGGTAGCTGAATATACAAACAAAGGGGATGCCCTTGCCAA
CCGAGTCCAGAACACTCTTGGAAGTTATGATGAAATGAAGGATCTGCTATCTAACCATTC
CAGTCAGAATCATCTGGTGGGCATTCCAAAGAATTCTGCACCCCAAACTCCCATCAGCAA
AAGTGAAGCAAGCTTTTATCCAGAACAAAAAAATCGAATGATCCCATCTCACCAGGAGAC
TACCCACTCCTCAACACCGATGCCTCCACCTTCAGTCGTGATACTGAATTCCACTCTAAT
ACACAGCAACAGAAAATCAAAATCTGAGTGGCCACGAGATAGTCATAACACTAGCCCTGC
ACAAGCAAGCCAGACCAGCAGTCAACCAAACAAGATGCAGACATCCACACAAGACCCACC
TCAAACCAGACTGGAAGACTTCTTTGTCTACCCTGCTGAGCAACCCCAAATTGGTACAGT
TGAAAAATCTAACCCATCTTCAAAGGAAGAAAATAACCCTAATTCAGGTGGAGAAGATAC
TTTCAAAGAAATTTTTCAGTCTAATTCACCAGAAGAATCTGAATTTACAGTGCAAGCACC
TGGGTCTCCCCTAGTTGCCTCATCTTTATTAGCTCCCAGCAGTGGCCTTTCAGTTCCAAC
ATTCCCACCAGGGCTTTACTGCAAAACAAGCATGGGACAACAAAAACCAACTGCATATGT
AAGGCCCATGGATGGGCAGGACCAGGCAACTGACATCTCTCCAACACTGAAGCCTTCCAT
TGAATTTGAGAACAGTTTTGGAAACCTCTCCTTTGGATCACTATTGGATGGGAAACCCAG
TGCAGTCAGTTCAAAGACTAAACTGCCAAAGTTCACAATCCTGCAAACAAGTGAAGTAAG
CCTTACCAGTGATCCAAGCTGTGTTGAAGAAATCTTGCGGGAATCTCAGCATCTGACTCC
AGGATTTACCTTACAAAAGTGGAGCGACCCAAGCAGCAGAGCTTCCACAAAGATGCTTGA
GGATGACTTGAAGCTGAGCAGTGATGAAGATGACCTTGAGCCTGTGAAGACCTTGACCAC
ACAGTGCACAGCCAACGAGCTTTACCAGGCTGTTGAAAAGGCAAAACCTAAGAATAATCC
TGTGAACCCACTCTTGGCCACACCACAGTCTACGCCTGCAACACAAACCAATGTGGGGTC
TGGCAGCTCCAGTGAGTCAGAGAGTAGCTCAGAATCGGACTCAGACACTGAAAGCAGCAC
CACTGACAGCGAATCCAATGAGGCTCCTAGAGTGGCTACTCCAGAGCCTGAGCCACCCTC
AACCAACAAATGGCAGCTAGACAAATGGCTGAACAAAGTCACGTCCCAGAACAAGTCATT
TATTTGTGGCCAAAATGAGACACCCACAGAGACCATTTCTCTGCCTCCTCCAATAATACA
ACCGGTGGAGGTCCAGGTCAAAGTGAAACCAAACCCTAGCCAGGCTGTGGCTGTACCCAA
AGAAAGGCCTCTCCTTAGTCTTATTAGGGAAAAAGCTCGACCAAGGCCCACACAGAAAAC
TCCAGAAACAAAGGCCTTGAAGCATAAGCTGTCAACATCAGTTGACACAGTTTCTCAAAG
GACAATTGGAAAGAAACAGCCCAAAAAAGTTGAGAAAAACACCAGCTTTGAGGAGTTTAC
CTGGCCCAAACCAAATATTACCAACAGCACTCCCAAAGAAAAAGGAAGTGTAGAGCTTCC
TGACCCACCACGAAGCCGAAACAAAGCTACTGCCCACAAACCAGTGCCTAGGAAAGAGCC
AAGGCCTCATGTCCCTCTGGCCACTGAGAAGAAGAAGTATAGAGGGCCTGGGAAGATTGT
CCCCAAGTCCCGGGAATTTATTGAAACAGATTCATCTACATCTGACTCCAACACAGACCA
GGAAGAGACCCTACAGATCAAAGTACTGCCACCTTGCATTACTTCTAAATCCAAGGAAAC
TTCTAATGCCAGCCTGACACTTAGCACCTTGACCAATGGCAACAGCAACAACTTATCCAC
CAGCAATGAGGAGACAGCTTTTTCCCCGCCTCCTGCCATGCAAACTGAGCTTTTGTCCCC
TCTACGGGATCATGAAAATCCAAAAAACCTCTGGGTGAAGATTGACCTCGACTTACTTTC
TAGAGTACCTGGCCAAAATTCAGTCCCAGTAACACCTGCAAAGACAGACTACAAGGAGAC
TGCCTCTAAACCCAAGCGTCAAACTGCTGCCACAGCTGTGGAAAAACCAGCCCCTAAGGG
CAAACGTAAGCACAAGCCAGCAGAAACTGCAGAGAAAATCCCTGAGAAGAAGCAGCGCCT
GGAGGACAACACTACTATCTGCCTGCTTCCCCCTTGCATCTCACCAGCCCCGCCCCACAA
GCCTCCCAGCACTAGAGAAAATTCATCCAGGAGAGCAAACAGGAAAAAAGAAGAAAAGCT
GTTCCCTCCTGCACTTTCCCCACTGGCAGAGGATCCTCCACGTCGCAGAAATGTCAGTGG
CAACAATGGGCATTTCGGTCAGGACAAAAACATTTCCATGGCTGGACAAATCACCTCTAG
CAAACCTAAAAGAAGTGAAGGCAAATTCTGTGCTACTTTCAAGGGGATATCTATAAATGA
GGGAGATGCTCCAAAAAAGGCTGCCTCTGCCACCGTTACTGTCGCCAATATGGCTCTTGC
CACTGCTACTGCCACTGCCACTGTTCCTGCCATTGTCACTGCCACTGTCACAGCTACTGC
CACCACCACTGCTACAGCCACTACCACAACCACTACCACTACTATTTCTAGCATCACCCC
TACAATCACTTCTGGCCTTATGGATAGCAGTCACCTGGAGATGACATCCTGGGCAGCCCT
GCCCCTTCTGTCCAGCAGTAGTGCCAATGTCCGGAGACCCAAGCTCACATTTGATGACTC
TGTTCACAATGCTGATTTTTACATGCAAGAGGCTAAGAAGCTGAAACACAAAGCTGATGC
ACTGTTTGAGAAATTTGGCAAAGCTGTGAATTATGCAGATGCAGCCTTGTCCTTCACTGA
ATGTGGCAATGCCATGGAACGTGATCCTCTGGAAGCGAAGTCCCCATATACCATGTATTC
TGAGACTGTGGAGCTCCTCAGGTATGCAATGAGACTGAAGAACTTTGCAAGCCCCTTGGC
TTCTGATGGGGATAAAAAGCTAGCCGTATTATGCTACCGATGCTTGTCACTTCTCTATTT
GAGGATGTTTAAACTGAAGAAGGACCATGCTATGAAGTACTCCAGATCACTGATGGAATA
TTTTAAGCAAAATGCTTCAAAAGTTACTCAGATACCATCTCCTTGGGTAGGCAATGGAAA
GAATACTCCATCCCCAGTGTCTCTCAACAATGTCTCTCCCATCAACTCAGTGGGGAATTG
TAACAATGGCCCTGTCACAATTCCCCAGCGCATCCACCATATGGCTGCTAGCCATGTGAA
TATTACCAGTAATGTGCTTCGGGGCTATGAACACTGGGATATGGCTGACAAACTGACAAG
AGACAATAAAGAATTCTTTGGTGATCTGGATACACTGATGGGGCCTCTGACCCAGCACAG
CAGCATGACCAATCTTGTCCGCTATGTTCGCCAGGGACTGTGTTGGCTACGCATCGATGC
TCATTTGTTGTAGGGCCTCATGGGCCCAGCTTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MDLFDFFRDWDLEQQCHYEQDRSALKKREWERRNQEVQQEEDLFSSGFDLFGEPYKVAEY TNKGDALANRVQNTLGSYDEMKDLLSNHSSQNHLVGIPKNSAPQTPISKSEASFYPEQKN RMIPSHQETTHSSTPMPPPSVVILNSTLIHSNRKSKSEWPRDSHNTSPAQASQTSSQPNK MQTSTQDPPQTRLEDFFVYPAEQPQIGTVEKSNPSSKEENNPNSGGEDTFKEIFQSNSPE ESEFTVQAPGSPLVASSLLAPSSGLSVPTFPPGLYCKTSMGQQKPTAYVRPMDGQDQATD ISPTLKPSIEFENSFGNLSFGSLLDGKPSAVSSKTKLPKFTILQTSEVSLTSDPSCVEEI LRESQHLTPGFTLQKWSDPSSRASTKMLEDDLKLSSDEDDLEPVKTLTTQCTANELYQAV EKAKPKNNPVNPLLATPQSTPATQTNVGSGSSSESESSSESDSDTESSTTDSESNEAPRV ATPEPEPPSTNKWQLDKWLNKVTSQNKSFICGQNETPTETISLPPPIIQPVEVQVKVKPN PSQAVAVPKERPLLSLIREKARPRPTQKTPETKALKHKLSTSVDTVSQRTIGKKQPKKVE KNTSFEEFTWPKPNITNSTPKEKGSVELPDPPRSRNKATAHKPVPRKEPRPHVPLATEKK KYRGPGKIVPKSREFIETDSSTSDSNTDQEETLQIKVLPPCITSKSKETSNASLTLSTLT NGNSNNLSTSNEETAFSPPPAMQTELLSPLRDHENPKNLWVKIDLDLLSRVPGQNSVPVT PAKTDYKETASKPKRQTAATAVEKPAPKGKRKHKPAETAEKIPEKKQRLEDNTTICLLPP CISPAPPHKPPSTRENSSRRANRKKEEKLFPPALSPLAEDPPRRRNVSGNNGHFGQDKNI SMAGQITSSKPKRSEGKFCATFKGISINEGDAPKKAASATVTVANMALATATATATVPAI VTATVTATATTTATATTTTTTTTISSITPTITSGLMDSSHLEMTSWAALPLLSSSSANVR RPKLTFDDSVHNADFYMQEAKKLKHKADALFEKFGKAVNYADAALSFTECGNAMERDPLE AKSPYTMYSETVELLRYAMRLKNFASPLASDGDKKLAVLCYRCLSLLYLRMFKLKKDHAM KYSRSLMEYFKQNASKVTQIPSPWVGNGKNTPSPVSLNNVSPINSVGNCNNGPVTIPQRI HHMAASHVNITSNVLRGYEHWDMADKLTRDNKEFFGDLDTLMGPLTQHSSMTNLVRYVRQ GLCWLRIDAHLL*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
4280
Start on reference sequence
462
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
