Gene:
Gene Symbol:
SALL1
Gene Name:
spalt-like transcription factor 1
Original Clone ID:
FLH481324.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00295069
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGTCGCGGAGGAAGCAAGCGAAGCCTCAACATTTCCAATCCGACCCCGAAGTGGCCTCG
CTCCCCCGGCGAGATGGAGACACAGAAAAGGGTCAACCGAGTCGCCCTACTAAGAGCAAG
GATGCCCACGTCTGTGGCCGGTGCTGTGCCGAGTTCTTTGAATTATCAGATCTTCTGCTC
CACAAGAAGAACTGTACTAAAAATCAATTAGTTTTAATCGTAAATGAAAATCCAGCCTCC
CCACCCGAAACCTTCTCCCCCAGCCCCCCTCCTGATAATCCTGATGAACAAATGAATGAC
ACAGTTAACAAAACAGATCAAGTGGACTGCAGCGACCTTTCAGAACACAACGGACTTGAC
AGGGAAGAGTCCATGGAGGTGGAGGCCCCGGTTGCTAACAAAAGCGGCAGCGGCACTTCC
AGCGGCAGCCACAGCAGTACCGCCCCAAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCGGC
GGCGGCGGCAGCTCCTCCACAGGTACCTCAGCGATCACAACCTCTCTACCTCAACTCGGG
GACCTGACAACACTGGGCAACTTCTCCGTAATCAACAGCAACGTCATCATCGAGAACCTC
CAGAGCACCAAGGTGGCGGTGGCCCAGTTCTCCCAGGAAGCGAGGTGCGGCGGGGCCTCT
GGGGGCAAGCTGGCCGTCCCAGCCCTCATGGAACAACTCCTAGCTCTGCAGCAGCAGCAG
ATCCACCAGCTGCAATTGATCGAACAGATTCGTCACCAAATATTGCTGTTGGCTTCTCAG
AATGCAGACTTGCCAACATCTTCTAGTCCTTCTCAAGGTACTTTACGAACATCTGCCAAC
CCCTTGTCCACGCTAAGTTCCCATTTATCTCAGCAGCTGGCAGCAGCAGCTGGATTGGCA
CAGAGCCTCGCCAGCCAATCTGCCAGCATTAGTGGTGTGAAACAGCTACCCCCAATCCAG
CTACCTCAGAGCAGTTCTGGCAACACCATCATTCCATCCAACAGCGGCTCTTCTCCCAAT
ATGAACATATTGGCAGCGGCAGTTACCACCCCGTCCTCTGAAAAAGTGGCTTCAAGTGCT
GGGGCCTCCCATGTCAGCAACCCAGCGGTCTCATCATCGTCCTCACCAGCTTTTGCAATA
AGCAGTTTATTAAGTCCTGCGTCTAATCCACTTCTACCTCAGCAAGCCTCCGCTAACTCG
GTTTTCCCCAGCCCTTTGCCCAACATCGGAACAACTGCAGAGGATTTAAACTCCTTGTCT
GCCTTGGCCCAGCAAAGAAAAAGCAAGCCACCAAATGTCACTGCCTTTGAAGCGAAAAGT
ACTTCCGATGAGGCATTCTTCAAACACAAGTGCAGGTTCTGCGCGAAGGTCTTTGGGAGT
GACAGTGCCTTGCAGATCCACTTGCGTTCCCATACCGGAGAGAGGCCATTCAAGTGCAAC
ATCTGCGGGAACAGGTTCTCCACCAAGGGGAATCTGAAAGTCCACTTTCAGCGCCACAAA
GAGAAATACCCTCATATCCAGATGAACCCCTATCCTGTGCCTGAGCATTTGGACAATATC
CCCACGAGTACTGGCATCCCATATGGCATGTCCATCCCTCCAGAGAAGCCAGTCACCAGC
TGGCTAGACACCAAACCAGTCCTGCCTACTCTGACCACTTCAGTCGGCCTGCCGTTGCCC
CCAACCCTCCCAAGCCTCATACCCTTCATCAAGACGGAAGAGCCAGCCCCCATCCCCATC
AGCCATTCTGCCACCAGCCCCCCAGGCTCAGTCAAAAGTGACTCCGGGGGCCCTGAGTCA
GCCACAAGAAACCTAGGTGGGCTCCCAGAGGAAGCCGAAGGGTCCACTCTGCCACCCTCT
GGTGGCAAAAGCGAAGAGAGTGGCATGGTCACCAACTCAGTCCCGACGGCGAGCAGTAGC
GTCCTGAGCTCCCCAGCGGCAGACTGCGGCCCCGCGGGCAGTGCCACCACCTTCACCAAC
CCTTTGTTGCCGCTCATGTCCGAGCAGTTCAAGGCCAAGTTTCCTTTTGGGGGACTCCTG
GACTCAGCTCAGGCATCAGAGACGTCCAAGCTTCAGCAACTGGTAGAAAACATTGACAAG
AAGGCCACTGACCCCAATGAGTGCATCATCTGCCACCGGGTTCTCAGCTGCCAGAGCGCC
TTGAAAATGCACTACAGGACACACACTGGGGAGAGGCCCTTTAAGTGTAAGATCTGTGGC
CGGGCTTTCACCACGAAAGGGAATCTTAAAACCCACTACAGTGTCCATCGTGCTATGCCC
CCGCTCAGAGTCCAGCATTCCTGCCCCATCTGCCAGAAGAAGTTCACGAACGCTGTGGTC
CTGCAGCAGCACATCCGAATGCATATGGGAGGCCAGATCCCCAACACCCCAGTCCCCGAC
AGCTACTCTGAGTCCATGGAGTCTGACACAGGTTCCTTTGATGAGAAAAATTTTGATGAC
CTAGACAACTTCTCTGATGAAAACATGGAAGACTGTCCTGAGGGCAGCATCCCTGATACA
CCTAAGTCTGCAGACGCCTCCCAAGACAGCTTATCCTCTTCGCCTTTGCCCCTCGAGATG
TCGAGCATCGCTGCTTTGGAAAATCAGATGAAGATGATCAATGCTGGCCTGGCAGAGCAG
CTACAGGCCAGCCTGAAGTCAGTGGAGAATGGGTCCATCGAGGGGGATGTCCTGACCAAT
GATTCATCCTCAGTGGGTGGTGACATGGAAAGCCAAAGTGCTGGCAGCCCAGCCATCTCA
GAGTCTACCTCTTCCATGCAGGCTCTGTCCCCGTCCAACAGCACGCAGGAGTTCCACAAG
TCACCCAGCATTGAGGAGAAACCACAGAGAGCGGTCCCAAGCGAGTTTGCCAATGGTTTG
TCTCCCACCCCAGTGAATGGTGGGGCTTTGGATTTGACATCTAGTCACGCAGAGAAAATC
ATCAAAGAAGATTCTTTGGGGATCCTCTTCCCTTTTAGAGACCGGGGTAAATTTAAAAAC
ACTGCTTGTGACATTTGTGGCAAAACATTTGCTTGTCAGAGTGCCTTGGACATTCACTAT
AGAAGTCATACCAAAGAGAGACCATTTATTTGCACAGTTTGCAATCGTGGCTTTTCCACA
AAGGGTAATTTGAAGCAGCACATGTTGACACATCAGATGCGAGATCTGCCATCCCAGCTC
TTTGAGCCCAGTTCCAACCTTGGCCCCAATCAGAACTCAGCGGTGATTCCCGCCAACTCG
TTGTCATCTCTCATCAAGACAGAGGTCAACGGCTTCGTGCATGTTTCTCCTCAGGACAGT
AAGGACACCCCCACCAGTCACGTCCCGTCTGGGCCTCTGTCTTCCTCTGCCACATCCCCA
GTTCTGCTCCCTGCTCTGCCCAGGAGAACTCCCAAGCAGCACTACTGCAACACATGTGGC
AAAACCTTCTCCTCATCGAGTGCCCTGCAGATTCACGAGAGAACTCACACTGGAGAGAAA
CCCTTTGCTTGCACTATTTGTGGAAGAGCTTTCACGACTAAAGGCAATCTTAAGGTACAC
ATGGGCACTCACATGTGGAATAGCACCCCTGCACGACGGGGTCGGCGGCTCTCTGTGGAT
GGCCCCATGACATTTCTAGGAGGCAATCCCGTCAAGTTCCCAGAAATGTTCCAGAAGGAT
TTGGCGGCAAGATCAGGAAGTGGGGATCCTTCCAGCTTCTGGAATCAGTATGCAGCAGCG
CTCTCCAACGGGCTGGCGATGAAGGCCAACGAGATCTCCGTCATTCAGAACGGTGGCATC
CCTCCAATTCCTGGAAGCCTCGGCAGTGGGAACAGCTCACCTGTTAGTGGGCTGACGGGA
AACCTGGAGAGGCTCCAGAACTCAGAGCCCAATGCTCCCCTGGCCGGCCTGGAGAAAATG
GCAAGCAGTGAGAACGGAACCAACTTCCGCTTCACCCGCTTCGTGGAGGACAGCAAGGAG
ATCGTCACGAGT
Protein sequence predicted based on nucleotide sequence
MSRRKQAKPQHFQSDPEVASLPRRDGDTEKGQPSRPTKSKDAHVCGRCCAEFFELSDLLL HKKNCTKNQLVLIVNENPASPPETFSPSPPPDNPDEQMNDTVNKTDQVDCSDLSEHNGLD REESMEVEAPVANKSGSGTSSGSHSSTAPSSSSSSSSSSGGGGSSSTGTSAITTSLPQLG DLTTLGNFSVINSNVIIENLQSTKVAVAQFSQEARCGGASGGKLAVPALMEQLLALQQQQ IHQLQLIEQIRHQILLLASQNADLPTSSSPSQGTLRTSANPLSTLSSHLSQQLAAAAGLA QSLASQSASISGVKQLPPIQLPQSSSGNTIIPSNSGSSPNMNILAAAVTTPSSEKVASSA GASHVSNPAVSSSSSPAFAISSLLSPASNPLLPQQASANSVFPSPLPNIGTTAEDLNSLS ALAQQRKSKPPNVTAFEAKSTSDEAFFKHKCRFCAKVFGSDSALQIHLRSHTGERPFKCN ICGNRFSTKGNLKVHFQRHKEKYPHIQMNPYPVPEHLDNIPTSTGIPYGMSIPPEKPVTS WLDTKPVLPTLTTSVGLPLPPTLPSLIPFIKTEEPAPIPISHSATSPPGSVKSDSGGPES ATRNLGGLPEEAEGSTLPPSGGKSEESGMVTNSVPTASSSVLSSPAADCGPAGSATTFTN PLLPLMSEQFKAKFPFGGLLDSAQASETSKLQQLVENIDKKATDPNECIICHRVLSCQSA LKMHYRTHTGERPFKCKICGRAFTTKGNLKTHYSVHRAMPPLRVQHSCPICQKKFTNAVV LQQHIRMHMGGQIPNTPVPDSYSESMESDTGSFDEKNFDDLDNFSDENMEDCPEGSIPDT PKSADASQDSLSSSPLPLEMSSIAALENQMKMINAGLAEQLQASLKSVENGSIEGDVLTN DSSSVGGDMESQSAGSPAISESTSSMQALSPSNSTQEFHKSPSIEEKPQRAVPSEFANGL SPTPVNGGALDLTSSHAEKIIKEDSLGILFPFRDRGKFKNTACDICGKTFACQSALDIHY RSHTKERPFICTVCNRGFSTKGNLKQHMLTHQMRDLPSQLFEPSSNLGPNQNSAVIPANS LSSLIKTEVNGFVHVSPQDSKDTPTSHVPSGPLSSSATSPVLLPALPRRTPKQHYCNTCG KTFSSSSALQIHERTHTGEKPFACTICGRAFTTKGNLKVHMGTHMWNSTPARRGRRLSVD GPMTFLGGNPVKFPEMFQKDLAARSGSGDPSSFWNQYAAALSNGLAMKANEISVIQNGGI PPIPGSLGSGNSSPVSGLTGNLERLQNSEPNAPLAGLEKMASSENGTNFRFTRFVEDSKE IVTS
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
32
End on reference sequence
4006
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
