Gene:
Gene Symbol:
THADA
Gene Name:
thyroid adenoma associated
Original Clone ID:
FLH481340.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00295085
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGGGTGTAAAGAAGAAGAAAGAAAT
GCAAGTTGCTGCGCTGACCATTTGCCATCAGGACCTTGAAACTTTGAAATCTTTTGCTGA
TGTGGAAGGGAAAAATCTAGCTTCTTTGCTGTTACATTGTGTGCAACTCACGGATGGAGT
GTCACAAATCCATTATATTAAACAGATTGTGCCTCTGCTGGAGAAAGCAGATAAAAATGG
CATGTGTGATCCCACTATTCAAAGTTGTTTGGATATCTTAGCAGGCATTTATCTTTCTTT
GAGTCTAAAGAATCCCTTGAAGAAAGTATTGGCAAGCTCACTAAATAGCCTGCCTGATTT
TTTTCTACCTGAGGCTATGCACCGTTTTACTTCTCGTCTTCAGGAAGAATTGAATACTAC
TGACTTATACTCTTACAGGAAAGTTACTGACAATATTTCTTCCTGTATGGAGAACTTTAA
CTTGGGTAGAGCAAGTGTTAATAATCTGCTTAAAAATGTGCTTCATTTTCTGCAGAAGAG
TTTAATTGAAATCCTGGAAGAAAATAGAAAATGTGCTGGAAATCATATTATTCAAACACA
GTTGATGAATGACTTACTGGTAGGCATTAGAGTTTCAATGATGTTAGTACAGAAAGTACA
AGATTTCCAGGGAAATCTTTGGAAGACTTCCGATTCTCCCATATGGCAAAATATGTGTGG
ATTGCTGAGTATTTTTACCAAGGTTTTAAGCGATGATGATCTGTTACAGACTGTACAGAG
CACATCTGGATTAGCTATTATTCTTTTTATTAAGACTATGTTTCACCCGTCTGAAAAGAT
TCCTCATTTGATTAGCAGTGTGCTGCTTCGTTCAGTGGACTGCACCAGTGTCCCCGAGTG
GTTTATGAGCAGCTGCAGGAGCCTCTGTTGTGGTGACATCTCTCAGTCAGCTGTCTTATT
CCTCTGTCAGGGGACACTTGCCATGTTGGACTGGCAGAACGGAAGCATGGGTCGGAGTGG
GGAGGCCCTGCTCTTGGATACTGCACATGTTTTGTTCACCTTGAGTTCACAGATTAAAGA
GCCAACGCTGGAAATGTTTCTGTCTAGAATCTTAGCATCCTGGACTAATTCAGCCATACA
AGTCCTTGAATCAAGTTCCCCGAGCCTAACGGACAGCCTGAATGGGAATTCAAGTATAGT
TGGGAGACTTTTGGAATATGTCTATACCCATTGGGAACATCCATTGGATGCTCTGAGACA
CCAAACCAAAATCATGTTCAAAAACCTTCTCCAAATGCACCGGCTCACTGTGGAAGGTGC
AGATTTCGTCCCTGATCCTTTCTTTGTGGAATTGACTGAGAGTCTTTTACGATTGGAATG
GCATATTAAAGGAAAGTACACGTGCCTTGGTTGTTTGGTAGAGTGCATAGGAGTTGAACA
TATTTTGGCTATAGATAAAACTATTCCATCTCAAATCTTAGAGGTGATGGGAGACCAGTC
ATTGGTACCTTATGCAAGTGACCTCTTGGAAACCATGTTTAGAAATCATAAGAGTCATTT
GAAATCCCAGACTGCTGAGAGTTCTTGGATTGACCAGTGGCATGAGACTTGGGTTTCTCC
TCTCCTTTTTATATTGTGTGAAGGAAACTTGGATCAAAAATCTTACGTGATTGATTATTA
CTTGCCAAAATTATTAAGTTACAGCCCTGAAAGCTTACAGTACATGGTAAAGATTCTTCA
GACTTCTATTGATGCTAAAACTGGACAAGAGCAATCTTTCCCATCCTTAGGGTCTTGTAA
TAGCAGGGGGGCTCTGGGAGCTTTGATGGCATGTCTGCGAATAGCTAGAGCTCATGGACA
TCTTCAGTCTGCAACTGATACCTGGGAGAACCTCGTGTCTGATGCAAGAATAAAGCAAGG
CTTAATTCATCAGCATTGCCAAGTAAGGATAGATACATTAGGCTTGCTTTGTGAAAGTAA
TCGGAGCACAGAAATTGTTTCCATGGAAGAAATGCAGTGGATTCAGTTCTTTATTACATA
CAATCTTAACAGCCAGTCTCCAGGAGTGCGGCAACAGATCTGTTCTCTTCTTAAAAAGTT
GTTTTGTAGGATACAGGAAAGTTCTCAGGTACTTTATAAATTGGAGCAGAGTAAATCCAA
ACGTGAACCAGAGAATGAGTTAACCAAACAGCACCCTTCTGTTTCTTTACAGCAGTATAA
GAATTTCATGTCATCCATTTGTAACAGTCTTTTTGAAGCATTGTTTCCTGGATCTTCCTA
CTCGACTAGATTTTCAGCTTTAACCATTTTAGGTTCAATAGCTGAAGTTTTTCATGTCCC
AGAAGGCAGAATTTATACAGTATATCAGCTGAGTCATGATATTGATGTTGGTCGTTTCCA
AACACTAATGGAATGTTTTACCAGCACTTTTGAAGACGTGAAAATTTTAGCATTTGATCT
TCTGATGAAGTTATCAAAAACAGCTGTACATTTTCAGGATTCGGGGAAACTGCAAGGCTT
ATTTCAGGCAGCATTGGAGCTCAGCACAAGCACCAAACCATACGACTGTGTGACAGCTTC
CTACCTGCTGAACTTCTTAATCTGGCAGGATGCTCTACCGTCATCCTTGTCTGCCTACTT
AACTCAGCAAGTTGCATGTGATAATGGAGATAGGCCTGCTGCTGTGGTGGAAAGGAACAC
ATTAATGGTTATCAAATGCTTGATGGAAAATCTTGAGGAAGAAGTATCTCAGGCTGAAAA
TTCTCTGCTTCAGGCAGCAGCAGCATTTCCAATGTATGGGCGAGTCCACTGTATAACAGG
AGCTTTGCAGAAGTTATCTCTAAACAGCCTGCAGTTGGTGAGCGAGTGGAGACCTGTGGT
AGAGAAGCTCCTTTTGATGTCCTACAGGCTTTCCACTGTGGTGTCTCCAGTCATTCAGAG
CTCATCCCCTGAAGGCCTCATCCCAATGGACACTGATTCAGAGTCAGCAAGCCGCTTACA
GATGATTCTGAATGAGATTCAGCCTCGAGATACTAATGATTATTTTAACCAAGCCAAAAT
ATTGAAAGAACATGATAGCTTTGATATGAAGGACTTGAATGCTAGTGTGGTGAATATTGA
TACTTCTACAGAAATCAAAGGTAAAGAAGTAAAAACATGTGATGTAACTGCGCAGATGGT
GCTGGTATGTTGTTGGAGAAGTATGAAGGAAGTTGCTTTACTTTTAGGCATGTTGTGCCA
GCTTCTGCCCATGCAGCCTGTGCCAGAATCTTCTGATGGATTATTGACGGTGGAGCAGGT
AAAAGAAATAGGAGATTACTTTAAACAACACCTTTTGCAGTCCAGGCACAGAGGAGCATT
TGAATTGGCTTATACTGGTTTTGTGAAACTCACTGAAGTACTAAACAGGTGCCCAAATGT
GAGTCTGCAAAAGCTGCCAGAACAGTGGCTATGGAGTGTTTTAGAGGAAATTAAATGCAG
TGATCCTTCATCTAAACTCTGTGCTACAAGGCGCAGTGCTGGAATTCCTTTCTACATACA
GGCACTGTTGGCATCTGAACCAAAGAAAGGCAGAATGGATTTGTTGAAAATAACAATGAA
AGAGTTAATCTCTTTGGCTGGGCCTACAGATGACATACAGAGTACAGTCCCCCAGGTTCA
TGCTTTAAATATCCTTAGAGCATTGTTCAGAGATACGCGCCTGGGAGAAAATATTATTCC
TTATGTTGCTGATGGAGCTAAGGCTGCAATTCTGGGTTTTACATCACCGGTCTGGGCAGT
GCGAAATTCATCCACACTTCTCTTTAGTGCCTTGATCACAAGAATTTTTGGAGTTAAAAG
GGCAAAGGATGAACATTCCAAAACAAATAGAATGACAGGGAGAGAGTTTTTCTCTCGTTT
CCCAGAACTCTATCCTTTTCTTCTCAAACAGTTGGAAACTGTAGCCAATACAGTAGACAG
TGATATGGGAGAACCAAATCGTCATCCAAGCATGTTTCTCTTACTTTTGGTGTTGGAGAG
ACTCTACGCTTCCCCGATGGATGGTACTTCTTCTGCTCTCAGCATGGGACCTTTTGTTCC
CTTCATTATGAGGTGTGGTCACTCACCTGTCTACCACTCCCGTGAAATGGCAGCTCGTGC
CTTGGTCCCATTTGTTATGATAGATCACATTCCTAATACCATTCGAACTCTGTTGTCCAC
ACTCCCCAGCTGCACTGACCAGTGTTTCCGGCAAAACCACATTCATGGGACACTTCTCCA
GGTTTTTCATTTGTTGCAAGCCTACTCAGACTCCAAACACGGAACGAATTCAGACTTCCA
GCACGAGCTGACTGACATCACTGTTTGTACCAAAGCCAAACTCTGGCTGGCCAAGAGGCA
AAATCCATGTTTGGTGACCAGAGCTGTATATATTGATATTCTCTTCCTATTGACTTGCTG
CCTCAACAGATCTGCAAAGGACAACCAGCCAGTTCTGGAGAGTCTTGGCTTCTGGGAGGA
AGTCAGAGGGATTATCTCAGGATCAGAGCTGATAACGGGATTCCCTTGGGCCTTCAAGGT
GCCAGGCCTGCCCCAGTACCTCCAGAGCCTCACCAGACTAGCCATTGCTGCAGTGTGGGC
CGCGGCAGCCAAGAGTGGAGAGCGGGAGACGAATGTCCCCATCTCTTTCTCTCAGCTGTT
AGAATCTGCCTTCCCTGAAGTGCGCTCACTAACACTGGAAGCCCTCTTGGAAAAGTTCTT
AGCAGCAGCCTCTGGACTTGGAGAGAAGGGCGTGCCACCCTTGCTGTGCAACATGGGAGA
GAAGTTCTTATTGTTGGCCATGAAGGAAAATCACCCAGAATGCTTCTGCAAGATACTGAA
AATTCTCCACTGCATGGACCCTGGTGAGTGGCTTCCCCAGACGGAGCACTGTGTCCATCT
GACCCCAAAGGAGTTCTTGATCTGGACGATGGATATTGCTTCCAATGAAAGATCTGAAAT
TCAGAGTGTAGCTCTGAGACTTGCTTCCAAAGTCATTTCCCACCACATGCAGACATGTGT
GGAGAACAGGGAATTGATAGCTGCTGAGCTGAAGCAGTGGGTTCAGCTGGTCATCTTGTC
ATGTGAAGACCATCTTCCTACAGAGTCTAGGCTGGCCGTCGTTGAAGTCCTCACCAGTAC
TACACCACTTTTCCTCACCAACCCCCATCCTATTCTTGAGTTGCAGGATACACTTGCTCT
CTGGAAGTGTGTCCTTACCCTTCTGCAGAGTGAGGAGCAAGCTGTTAGAGATGCAGCCAC
GGAAACCGTGACAACTGCCATGTCACAAGAAAATACCTGCCAGTCAACAGAGTTTGCCTT
CTGCCAGGTGGATGCCTCCATCGCTCTGGCCCTGGCCCTGGCCGTCCTGTGTGATCTGCT
CCAGCAGTGGGACCAGTTGGCCCCTGGACTGCCCATCCTGCTGGGATGGCTGTTGGGAGA
GAGTGATGACCTCGTGGCCTGTGTGGAGAGCATGCATCAGGTGGAAGAAGACTACCTGTT
TGAAAAAGCAGAAGTCAACTTTTGGGCCGAGACCCTGATCTTTGTGAAATACCTCTGCAA
GCACCTCTTCTGTCTCCTCTCAAAGTCCGGCTGGCGTCCCCCAAGCCCTGAGATGCTCTG
TCACCTTCAAAGGATGGTGTCAGAGCAGTGCCACCTCCTGTCTCAGTTCTTCAGAGAGCT
TCCACCAGCTGCTGAGTTTGTGAAGACAGTGGAGTTCACAAGACTACGCATTCAAGAGGA
AAGGACTTTGGCTTGCTTGAGGCTGCTGGCCTTTTTGGAAGGAAAGGAAGGGGAAGACAC
CCTAGTTCTCAGTGTTTGGGACTCTTATGCAGAATCGAGGCAGTTAACTCTTCCAAGAAC
AGAAGCGGCATGTTAGGGCCTCATGGGCCCAGCTTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MGVKKKKEMQVAALTICHQDLETLKSFADVEGKNLASLLLHCVQLTDGVSQIHYIKQIVP LLEKADKNGMCDPTIQSCLDILAGIYLSLSLKNPLKKVLASSLNSLPDFFLPEAMHRFTS RLQEELNTTDLYSYRKVTDNISSCMENFNLGRASVNNLLKNVLHFLQKSLIEILEENRKC AGNHIIQTQLMNDLLVGIRVSMMLVQKVQDFQGNLWKTSDSPIWQNMCGLLSIFTKVLSD DDLLQTVQSTSGLAIILFIKTMFHPSEKIPHLISSVLLRSVDCTSVPEWFMSSCRSLCCG DISQSAVLFLCQGTLAMLDWQNGSMGRSGEALLLDTAHVLFTLSSQIKEPTLEMFLSRIL ASWTNSAIQVLESSSPSLTDSLNGNSSIVGRLLEYVYTHWEHPLDALRHQTKIMFKNLLQ MHRLTVEGADFVPDPFFVELTESLLRLEWHIKGKYTCLGCLVECIGVEHILAIDKTIPSQ ILEVMGDQSLVPYASDLLETMFRNHKSHLKSQTAESSWIDQWHETWVSPLLFILCEGNLD QKSYVIDYYLPKLLSYSPESLQYMVKILQTSIDAKTGQEQSFPSLGSCNSRGALGALMAC LRIARAHGHLQSATDTWENLVSDARIKQGLIHQHCQVRIDTLGLLCESNRSTEIVSMEEM QWIQFFITYNLNSQSPGVRQQICSLLKKLFCRIQESSQVLYKLEQSKSKREPENELTKQH PSVSLQQYKNFMSSICNSLFEALFPGSSYSTRFSALTILGSIAEVFHVPEGRIYTVYQLS HDIDVGRFQTLMECFTSTFEDVKILAFDLLMKLSKTAVHFQDSGKLQGLFQAALELSTST KPYDCVTASYLLNFLIWQDALPSSLSAYLTQQVACDNGDRPAAVVERNTLMVIKCLMENL EEEVSQAENSLLQAAAAFPMYGRVHCITGALQKLSLNSLQLVSEWRPVVEKLLLMSYRLS TVVSPVIQSSSPEGLIPMDTDSESASRLQMILNEIQPRDTNDYFNQAKILKEHDSFDMKD LNASVVNIDTSTEIKGKEVKTCDVTAQMVLVCCWRSMKEVALLLGMLCQLLPMQPVPESS DGLLTVEQVKEIGDYFKQHLLQSRHRGAFELAYTGFVKLTEVLNRCPNVSLQKLPEQWLW SVLEEIKCSDPSSKLCATRRSAGIPFYIQALLASEPKKGRMDLLKITMKELISLAGPTDD IQSTVPQVHALNILRALFRDTRLGENIIPYVADGAKAAILGFTSPVWAVRNSSTLLFSAL ITRIFGVKRAKDEHSKTNRMTGREFFSRFPELYPFLLKQLETVANTVDSDMGEPNRHPSM FLLLLVLERLYASPMDGTSSALSMGPFVPFIMRCGHSPVYHSREMAARALVPFVMIDHIP NTIRTLLSTLPSCTDQCFRQNHIHGTLLQVFHLLQAYSDSKHGTNSDFQHELTDITVCTK AKLWLAKRQNPCLVTRAVYIDILFLLTCCLNRSAKDNQPVLESLGFWEEVRGIISGSELI TGFPWAFKVPGLPQYLQSLTRLAIAAVWAAAAKSGERETNVPISFSQLLESAFPEVRSLT LEALLEKFLAAASGLGEKGVPPLLCNMGEKFLLLAMKENHPECFCKILKILHCMDPGEWL PQTEHCVHLTPKEFLIWTMDIASNERSEIQSVALRLASKVISHHMQTCVENRELIAAELK QWVQLVILSCEDHLPTESRLAVVEVLTSTTPLFLTNPHPILELQDTLALWKCVLTLLQSE EQAVRDAATETVTTAMSQENTCQSTEFAFCQVDASIALALALAVLCDLLQQWDQLAPGLP ILLGWLLGESDDLVACVESMHQVEEDYLFEKAEVNFWAETLIFVKYLCKHLFCLLSKSGW RPPSPEMLCHLQRMVSEQCHLLSQFFRELPPAAEFVKTVEFTRLRIQEERTLACLRLLAF LEGKEGEDTLVLSVWDSYAESRQLTLPRTEAAC*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
141
End on reference sequence
6002
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
