Gene:
Gene Symbol:
HIVEP2
Gene Name:
human immunodeficiency virus type I enhancer binding protein 2
Original Clone ID:
FLH481353.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00295098
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGACACTGGGGACACAGCTCTAGGACAAAAAGCTACCTCAAGGTCTGGAGAAACTGAT
AAAGCATCAGGTAGATGGAGACAGGAACAATCAGCTGTTATTAAGATGAGCACTTTTGGC
AGTCATGAAGGACAGCGGCAACCACAAATAGAGCCTGAGCAAATCGGAAACACAGCATCA
GCACAACTGTTTGGTTCTGGGAAACTGGCCTCCCCTAGTGAAGTGGTGCAGCAAGTCGCA
GAGAAGCAATATCCACCGCATCGTCCGAGTCCTTACTCATGCCAACACTCACTCTCTTTC
CCTCAGCACTCATTGCCACAGGGGGTCATGCACAGCACCAAGCCACATCAGAGCCTCGAA
GGTCCTCCGTGGCTTTTCCCTGGCCCTTTGCCATCCGTTGCCTCTGAGGACTTATTTCCT
TTTCCTATACATGGCCACAGTGGTGGTTATCCTAGAAAAAAGATTTCAAGTCTGAACCCT
GCTTATAGCCAATACTCCCAGAAAAGTATTGAACAGGCAGAAGAGGCTCACAAGAAAGAG
CACAAACCCAAAAAGCCTGGCAAGTACATTTGCCCTTACTGCAGCAGAGCGTGTGCCAAA
CCTAGTGTACTGAAAAAACACATCAGGTCCCATACTGGGGAGCGGCCATATCCATGTATA
CCTTGTGGTTTCTCTTTCAAGACAAAGAGCAATTTGTACAAGCACAGGAAGTCACATGCC
CATGCAATTAAGGCAGGATTAGTACCTTTCACAGAGTCAGCTGTATCTAAATTGGACCTA
GAGGCTGGTTTTATTGATGTAGAAGCAGAAATACATTCAGATGGTGAACAGAGTACAGAC
ACAGATGAGGAGAGTTCTTTATTTGCCGAGGCTTCTGACAAAATGAGTCCTGGTCCACCC
ATCCCACTGGACATTGCCAGCAGAGGCGGCTATCATGGGTCATTGGAAGAATCATTGGGA
GGTCCAATGAAGGTGCCGATTTTGATTATCCCTAAAAGTGGGATTCCTCTCCCTAATGAA
AGCTCTCAGTATATTGGCCCTGATATGCTACCAAATCCATCTTTAAATACTAAGGCTGAT
GATTCGCACACAGTCAAACAGAAACTTGCACTAAGACTGTCAGAGAAAAAAGGACAAGAT
TCTGAGCCATCGCTCAACCTTCTGAGCCCGCACAGTAAAGGAAGCACTGATTCTGGTTAC
TTTTCTCGCTCAGAAAGTGCTGAGCAGCAAATAAGCCCTCCCAACACAAATGCAAAGTCT
TATGAAGAAATCATCTTTGGAAAATACTGTCGGCTTAGTCCGAGAAATGCACTCAGTGTT
ACAACCACAAGTCAGGAGCGTGCCGCAATGGGTAGGAAGGGCATAATGGAACCATTACCT
CACGTTAACACCAGGTTAGATGTCAAGATGTTTGAAGATCCTGTTTCACAGCTGATCCCA
AGCAAGGGAGATGTCGACCCCAGTCAAACGAGCATGCTGAAATCCACTAAGTTCAACAGT
GAGTCCAGACAACCCCAGATTATTCCATCATCTATCAGGAACGAAGGAAAACTTTATCCA
GCAAACTTCCAAGGCAGCAACCCGGTTCTCTTAGAAGCTCCTGTAGACTCTTCACCCCTT
ATTAGAAGCAACTCAGTGCCAACTTCTTCAGCAACTAATCTAACTATTCCTCCTTCTTTG
AGAGGAAGTCACTCATTTGATGAAAGGATGACTGGTTCCGACGATGTATTCTATCCAGGG
ACCGTGGGCATACCCCCTCAGCGCATGCTAAGAAGACAAGCGGCATTTGAGCTGCCTTCG
GTACAGGAGGGCCACGTGGAAGTCGAGCACCATGGCAGGATGTTGAAGGGTATCAGCAGT
TCATCCCTGAAGGAAAAGAAATTGTCTCCTGGGGACAGGGTTGGGTATGACTATGATGTC
TGTCGGAAACCCTATAAGAAGTGGGAGGACTCTGAAACACCAAAGCAAAACTACAGGGAC
ATTTCCTGCTTGAGTTCTTTAAAGCATGGTGGAGAATATTTCATGGATCCCGTGGTGCCA
TTGCAGGGAGTACCAAGCATGTTTGGAACTACCTGTGAAAACAGGAAACGCCGGAAAGAG
AAGAGCGTAGGGGATGAAGAGGACACGCCCATGATCTGCAGCAGCATTGTAAGCACTCCT
GTGGGCATCATGGCTTCCGATTATGACCCCAAACTGCAGATGCAGGAAGGAGTCAGGAGT
GGATTTGCCATGGCTGGACACGAAAACCTTTCTCATGGTCACACGGAACGCTTTGACCCA
TGTCGGCCCCAACTGCAGCCTGGAAGTCCATCTCTTGTGTCAGAGGAGTCACCTTCAGCC
ATTGATTCAGACAAGATGTCAGACCTAGGGGGCAGGAAACCTCCTGGAAATGTGATTTCT
GTGATTCAGCACACCAACTCACTGAGCCGACCCAATTCATTTGAAAGGTCTGAGTCAGCC
GAACTTGTGGCTTGCACACAGGATAAAGCCCCTTCCCCTTCAGAGACTTGTGACAGTGAG
ATTTCAGAAGCCCCAGTGAGTCCTGAGTGGGCTCCACCTGGGGATGGTGCAGAAAGTGGG
GGGAAACCCTCTCCATCTCAGCAGGTGCAGCAGCAGTCCTATCACACACAGCCCAGGCTA
GTTCGGCAACACAACATCCAGGTTCCTGAGATTCGAGTGACCGAGGAGCCTGATAAACCT
GAGAAGGAGAAGGAAGCCCAGAGCAAAGAGCCAGAGAAGCCTGTGGAAGAATTTCAGTGG
CCCCAGAGAAGTGAGACCCTTTCCCAGCTCCCCGCGGAGAAGTTGCCACCCAAAAAGAAG
CGTCTGCGACTTGCAGATATGGAGCACTCCTCAGGGGAGTCCAGCTTTGAATCCACAGGC
ACAGGCCTCTCCCGCAGCCCCAGCCAAGAAAGCAACTTGTCCCACAGCTCCAGTTTCTCC
ATGTCTTTTGAAAGAGAAGAAACCAGTAAGCTTTCTGCACTTCCTAAGCAGGATGAGTTT
GGGAAGCATTCAGAGTTTCTGACTGTCCCTGCTGGTTCATACTCATTGTCTGTCCCAGGC
CATCACCACCAGAAAGAGATGCGACGCTGCTCATCAGAGCAGATGCCTTGTCCTCACCCA
GCGGAAGTCCCAGAAGTTCGGAGCAAATCATTTGATTATGGGAATCTGTCCCATGCTCCT
GTGTCGGGAGCAGCAGCCTCCACGGTATCACCGTCCAGGGAGAGGAAGAAATGCTTTCTG
GTGCGGCAAGCTTCCTTCAGTGGCTCCCCAGAAATCTCCCAGGGCGAGGTTGGCATGGAT
CAGAGCGTGAAGCAAGAGCAGCTGGAGCACCTGCATGCTGGCCTCCGGTCCGGGTGGCAC
CATGGCCCGCCTGCTGTGCTGCCTCCTCTTCAGCAAGAGGACCCAGGGAAGCAGGTGGCG
GGTCCTTGTCCCCCGCTGAGCTCGGGGCCACTGCACCTGGCCCAGCCACAGATCATGCAC
ATGGACAGTCAGGAATCTTTGAGAAATCCCTTGATCCAACCAACATCCTATATGACAAGC
AAGCACTTACCTGAACAGCCACACTTATTTCCACATCAAGAGACAATTCCATTTTCTCCA
ATCCAGAATGCCTTGTTTCAGTTTCAGTATCCTACAGTTTGTATGGTTCATTTACCAGCT
CAGCAGCCTCCCTGGTGGCAGGCACATTTCCCACATCCCTTTGCTCAGCACCCTCAGAAG
AGCTATGGCAAGCCCTCTTTTCAGACAGAAATCCATTCGAGCTATCCCTTAGAGCATGTG
GCAGAGCACACTGGAAAGAAACCTGCTGAGTATGCACACACGAAAGAGCAGACCTACCCA
TGTTATTCAGGAGCATCAGGGCTACACCCAAAGAACCTTCTTCCAAAGTTTCCATCAGAC
CAGAGCAGTAAGTCAACTGAAACGCCCTCTGAGCAGGTTCTTCAAGAAGATTTTGCCTCG
GCAAATGCTGGGTCTTTGCAGTCCCTCCCAGGAACAGTGGTTCCTGTTCGGATCCAGACG
CACGTACCATCCTATGGAAGTGTCATGTACACAAGCATTTCTCAGATACTTGGGCAGAAT
AGCCCTGCCATTGTCATATGCAAAGTCGATGAGAATATGACCCAAAGGACACTGGTCACC
AACGCAGCCATGCAAGGGATAGGATTCAACATTGCCCAGGTGCTGGGGCAGCATGCGGGC
TTGGAGAAGTACCCCATTTGGAAAGCACCTCAGACTTTGCCCCTCGGCTTAGAATCCTCC
ATCCCCTTGTGTTTACCTTCCACCTCTGACAGCGTGGCCACCCTGGGAGGTAGCAAGCGA
ATGCTTTCTCCAGCCAGTAGCTTGGAGCTCTTCATGGAAACCAAGCAGCAGAAAAGGGTC
AAAGAAGAAAAGATGTACGGACAGATTGTGGAGGAGCTTAGTGCTGTGGAGCTGACCAAC
TCAGACATCAAAAAGGACCTCTCCCGCCCCCAGAAACCCCAGCTGGTTCGACAAGGATGT
GCTTCTGAGCCAAAAGATGGCTTGCAGTCAGGGTCATCTTCCTTCTCCTCGCTGTCGCCC
TCCTCATCTCAAGACTATCCTTCTGTTAGCCCGTCTTCCAGGGAGCCATTCCTGCCCAGC
AAGGAGATGCTTTCCGGTTCCCGGGCACCACTTCCGGGGCAGAAGTCCAGTGGGCCTTCT
GAAAGCAAAGAATCTTCAGATGAATTAGATATCGATGAGACGGCATCGGACATGAGCATG
AGCCCACAGAGTTCTTCATTACCAGCAGGAGATGGTCAGCTGGAAGAGGAAGGGAAGGGC
CACAAGCGGCCTGTTGGCATGCTGGTCCGCATGGCCTCTGCCCCCAGCGGGAACGTGGCA
GACTCAACTCTTCTTCTCACGGACATGGCAGATTTCCAGCAGATTCTTCAGTTCCCCAGT
CTGCGGACAACAACTACTGTGAGTTGGTGCTTCTTGAATTATACAAAACCCAATTATGTG
CAACAGGCCACCTTCAAATCCTCGGTTTATGCTTCATGGTGCATTAGTTCCTGTAATCCA
AACCCATCAGGATTGAACACCAAGACCACGCTGGCTCTTCTGAGGTCCAAGCAAAAAATC
ACTGCAGAAATTTATACTCTGGCTGCTATGCATAGGCCTGGAACCGGCAAGCTTACATCA
TCAAGTGCTTGGAAGCAGTTTACTCAGATGAAACCTGATGCGTCCTTTTTATTTGGCAGC
AAACTAGAAAGGAAACTAGTGGGAAATATCTTAAAGGAAAGAGGGAAAGGAGATATTCAT
GGAGATAAAGATATTGGATCCAAACAAACTGAGCCAATCCGAATTAAAATATTTGAAGGA
GGGTACAAATCGAATGAAGATTATGTATATGTCAGAGGACGTGGCCGGGGAAAGTACATT
TGTGAAGAATGTGGGATTCGCTGTAAGAAGCCAAGCATGCTCAAAAAACACATCCGTACC
CATACTGATGTTCGGCCTTATGTATGCAAGTTATGTAACTTTGCCTTCAAAACGAAAGGA
AACCTAACGAAGCATATGAAATCTAAAGCACACATGAAAAAATGCCTGGAATTGGGAGTC
TCAATGACATCGGTGGATGATACAGAAACTGAGGAAGCAGAAAATTTGGAAGATTTGCAC
AAAGCAGCAGAGAAGCATAGCATGTCCAGCATTTCAACTGATCATCAGTTCTCCGATGCT
GAGGAATCAGATGGTGAGGATGGAGATGATAATGATGATGATGATGAAGATGAAGATGAC
TTTGACGACCAGGGAGATTTAACACCAAAAACAAGATCAAGAAGCACCAGTCCTCAGCCT
CCTAGATTCTCCTCCTTGCCTGTGAATGTTGGCGCCGTACCCCACGGGGTTCCTTCAGAT
AGTTCCCTGGGACATTCTTCGTTGATCAGCTATTTGGTTACTTTGCCAAGTATTCGAGTT
ACTCAGCTTATGACACCCAGTGATTCATGTGAAGATACCCAGATGACAGAATACCAGAGG
CTATTCCAGAGCAAAAGTACGGACTCAGAACCAGACAAAGACAGATTGGACATACCTAGT
TGTATGGATGAGGAGTGCATGCTACCTTCAGAGCCAAGCTCCTCTCCCAGGGACTTCTCA
CCCTCAAGCCACCATTCCTCTCCAGGATATGATTCTTCACCCTGTCGAGATAATTCACCA
AAGAGGTATCTGATACCCAAAGGAGATTTATCTCCCAGGAGACATTTATCACCTAGGAGA
GATCTGTCACCCATGAGACATCTTTCACCAAGAAAGGAAGCTGCATTGAGAAGAGAGATG
TCCCAAAGAGATGTTTCACCAAGAAGGCATTTGTCTCCAAGGAGGCCAGTGTCTCCTGGG
AAAGATATCACAGCAAGAAGAGACCTCTCTCCTAGAAGAGAGAGAAGATACATGACCACA
ATAAGAGCGCCATCTCCCAGAAGGGCTTTATACCATAACCCACCATTGTCCATGGGACAG
TATTTGCAAGCAGAGCCAATTGTATTGGGGCCTCCTAATTTAAGAAGAGGATTACCTCAG
GTTCCTTACTTCAGTCTCTATGGAGACCAAGAAGGTGCTTATGAACATCCAGGCTCCAGC
CTTTTCCCTGAGGGTCCTAATGACTATGTCTTCAGTCATCTTCCACTCCACTCTCAGCAA
CAAGTGCGAGCCCCTATCCCCATGGTGCCCGTTGGTGGGATCCAGATGGTTCACTCCATG
CCGCCAGCCCTTTCCAGTTTACATCCTTCACCCACATTGCCCCTGCCAATGGAGGGCTTT
GAGGAGAAGAAAGGCGCGTCAGGGGAGTCCTTCTCCAAGGACCCCTATGTGCTTTCTAAG
CAGCATGAGAAGCGAGGTCCTCACGCTTTGCAGTCATCTGGTCCACCTAGCACTCCCTCC
TCTCCTCGGCTGTTGATGAAACAGAGCACTTCGGAAGACAGCCTAAACGCAACAGAGCGG
GAACAGGAGGAAAATATACAGACTTGTACAAAAGCCATTGCCTCTCTCCGGATTGCCACG
GAAGAGGCAGCTCTGCTCGGGCCAGATCAGCCAGCGCGGGTGCAGGAGCCCCACCAGAAC
CCCCTGGGAAGTGCACATGTTAGCATTAGACACTTTAGTAGACCTGAGCCAGGTCAGCCC
TGTACCTCAGCCACCCACCCTGACTTGCATGATGGTGAAAAGGACAATTTTGGTACATCA
CAGACTCCATTAGCTCACTCCACGTTTTACAGCAAGAGTTGTGTGGATGACAAGCAGTTG
GACTTTCACAGCAGCAAGGAATTATCTTCAAGCACAGAGGAAAGCAAAGATCCTTCATCA
GAAAAGAGTCAGCTACAT
Protein sequence predicted based on nucleotide sequence
MDTGDTALGQKATSRSGETDKASGRWRQEQSAVIKMSTFGSHEGQRQPQIEPEQIGNTAS AQLFGSGKLASPSEVVQQVAEKQYPPHRPSPYSCQHSLSFPQHSLPQGVMHSTKPHQSLE GPPWLFPGPLPSVASEDLFPFPIHGHSGGYPRKKISSLNPAYSQYSQKSIEQAEEAHKKE HKPKKPGKYICPYCSRACAKPSVLKKHIRSHTGERPYPCIPCGFSFKTKSNLYKHRKSHA HAIKAGLVPFTESAVSKLDLEAGFIDVEAEIHSDGEQSTDTDEESSLFAEASDKMSPGPP IPLDIASRGGYHGSLEESLGGPMKVPILIIPKSGIPLPNESSQYIGPDMLPNPSLNTKAD DSHTVKQKLALRLSEKKGQDSEPSLNLLSPHSKGSTDSGYFSRSESAEQQISPPNTNAKS YEEIIFGKYCRLSPRNALSVTTTSQERAAMGRKGIMEPLPHVNTRLDVKMFEDPVSQLIP SKGDVDPSQTSMLKSTKFNSESRQPQIIPSSIRNEGKLYPANFQGSNPVLLEAPVDSSPL IRSNSVPTSSATNLTIPPSLRGSHSFDERMTGSDDVFYPGTVGIPPQRMLRRQAAFELPS VQEGHVEVEHHGRMLKGISSSSLKEKKLSPGDRVGYDYDVCRKPYKKWEDSETPKQNYRD ISCLSSLKHGGEYFMDPVVPLQGVPSMFGTTCENRKRRKEKSVGDEEDTPMICSSIVSTP VGIMASDYDPKLQMQEGVRSGFAMAGHENLSHGHTERFDPCRPQLQPGSPSLVSEESPSA IDSDKMSDLGGRKPPGNVISVIQHTNSLSRPNSFERSESAELVACTQDKAPSPSETCDSE ISEAPVSPEWAPPGDGAESGGKPSPSQQVQQQSYHTQPRLVRQHNIQVPEIRVTEEPDKP EKEKEAQSKEPEKPVEEFQWPQRSETLSQLPAEKLPPKKKRLRLADMEHSSGESSFESTG TGLSRSPSQESNLSHSSSFSMSFEREETSKLSALPKQDEFGKHSEFLTVPAGSYSLSVPG HHHQKEMRRCSSEQMPCPHPAEVPEVRSKSFDYGNLSHAPVSGAAASTVSPSRERKKCFL VRQASFSGSPEISQGEVGMDQSVKQEQLEHLHAGLRSGWHHGPPAVLPPLQQEDPGKQVA GPCPPLSSGPLHLAQPQIMHMDSQESLRNPLIQPTSYMTSKHLPEQPHLFPHQETIPFSP IQNALFQFQYPTVCMVHLPAQQPPWWQAHFPHPFAQHPQKSYGKPSFQTEIHSSYPLEHV AEHTGKKPAEYAHTKEQTYPCYSGASGLHPKNLLPKFPSDQSSKSTETPSEQVLQEDFAS ANAGSLQSLPGTVVPVRIQTHVPSYGSVMYTSISQILGQNSPAIVICKVDENMTQRTLVT NAAMQGIGFNIAQVLGQHAGLEKYPIWKAPQTLPLGLESSIPLCLPSTSDSVATLGGSKR MLSPASSLELFMETKQQKRVKEEKMYGQIVEELSAVELTNSDIKKDLSRPQKPQLVRQGC ASEPKDGLQSGSSSFSSLSPSSSQDYPSVSPSSREPFLPSKEMLSGSRAPLPGQKSSGPS ESKESSDELDIDETASDMSMSPQSSSLPAGDGQLEEEGKGHKRPVGMLVRMASAPSGNVA DSTLLLTDMADFQQILQFPSLRTTTTVSWCFLNYTKPNYVQQATFKSSVYASWCISSCNP NPSGLNTKTTLALLRSKQKITAEIYTLAAMHRPGTGKLTSSSAWKQFTQMKPDASFLFGS KLERKLVGNILKERGKGDIHGDKDIGSKQTEPIRIKIFEGGYKSNEDYVYVRGRGRGKYI CEECGIRCKKPSMLKKHIRTHTDVRPYVCKLCNFAFKTKGNLTKHMKSKAHMKKCLELGV SMTSVDDTETEEAENLEDLHKAAEKHSMSSISTDHQFSDAEESDGEDGDDNDDDDEDEDD FDDQGDLTPKTRSRSTSPQPPRFSSLPVNVGAVPHGVPSDSSLGHSSLISYLVTLPSIRV TQLMTPSDSCEDTQMTEYQRLFQSKSTDSEPDKDRLDIPSCMDEECMLPSEPSSSPRDFS PSSHHSSPGYDSSPCRDNSPKRYLIPKGDLSPRRHLSPRRDLSPMRHLSPRKEAALRREM SQRDVSPRRHLSPRRPVSPGKDITARRDLSPRRERRYMTTIRAPSPRRALYHNPPLSMGQ YLQAEPIVLGPPNLRRGLPQVPYFSLYGDQEGAYEHPGSSLFPEGPNDYVFSHLPLHSQQ QVRAPIPMVPVGGIQMVHSMPPALSSLHPSPTLPLPMEGFEEKKGASGESFSKDPYVLSK QHEKRGPHALQSSGPPSTPSSPRLLMKQSTSEDSLNATEREQEENIQTCTKAIASLRIAT EEAALLGPDQPARVQEPHQNPLGSAHVSIRHFSRPEPGQPCTSATHPDLHDGEKDNFGTS QTPLAHSTFYSKSCVDDKQLDFHSSKELSSSTEESKDPSSEKSQLH
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
744
End on reference sequence
8084
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
