Gene:
Gene Symbol:
ITGA11
Gene Name:
integrin, alpha 11
Original Clone ID:
FLH481392.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00295137
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGACCTGCCCAGGGGCCTGGTGGTGGCCTGGGCGCTCAGCCTGTGGCCAGGGTTCACG
GACACCTTCAACATGGACACCAGGAAGCCCCGGGTCATCCCTGGCTCCAGGACCGCCTTC
TTTGGCTACACAGTGCAGCAGCACGACATCAGTGGCAATAAGTGGCTGGTCGTGGGCGCC
CCACTGGAAACCAATGGCTACCAGAAGACGGGAGACGTGTACAAGTGTCCAGTGATCCAC
GGGAACTGCACCAAACTCAACCTGGGAAGGGTCACCCTGTCCAACGTGTCCGAGCGGAAA
GACAACATGCGCCTCGGCCTTAGTCTCGCCACCAACCCCAAGGACAACAGCTTCCTGGCC
TGCAGCCCCCTCTGGTCTCATGAGTGTGGGAGCTCCTACTACACCACAGGGATGTGTTCA
AGAGTCAACTCCAACTTCAGGTTCTCCAAGACCGTGGCCCCAGCTCTCCAAAGGTGCCAG
ACCTACATGGACATCGTCATTGTCCTGGATGGCTCCAACAGCATCTACCCCTGGGTGGAG
GTTCAGCACTTCCTCATCAACATCCTGAAAAAGTTTTACATTGGCCCAGGGCAGATCCAG
GTTGGAGTTGTGCAGTATGGCGAAGATGTGGTGCATGAGTTTCACCTCAACGACTACAGG
TCTGTAAAAGATGTGGTGGAAGCTGCCAGCCACATTGAGCAGAGAGGAGGAACAGAGACC
CGGACGGCATTTGGCATTGAATTTGCACGCTCAGAGGCTTTCCAGAAGGGTGGAAGGAAA
GGAGCCAAGAAGGTGATGATTGTCATCACAGATGGGGAGTCCCACGACAGCCCAGACCTG
GAGAAGGTGATCCAGCAAAGCGAAAGAGACAACGTAACAAGATATGCGGTGGCCGTCCTG
GGCTACTACAACCGCAGGGGGATCAATCCAGAAACTTTTCTAAATGAAATCAAATACATC
GCCAGTGACCCTGATGACAAGCACTTCTTCAATGTCACTGATGAGGCTGCCTTGAAGGAC
ATTGTCGATGCCCTGGGGGACAGAATCTTCAGCCTGGAAGGCACCAACAAGAACGAGACC
TCCTTTGGGCTGGAGATGTCACAGACGGGCTTTTCCTCGCACGTGGTGGAGGATGGGGTT
CTGCTGGGAGCCGTCGGTGCCTATGACTGGAATGGAGCTGTGCTAAAGGAGACGAGTGCC
GGGAAGGTCATTCCTCTCCGCGAGTCCTACCTGAAAGAGTTCCCCGAGGAGCTCAAGAAC
CATGGTGCATACCTGGGGTACACAGTCACATCGGTCGTGTCCTCCAGGCAGGGGCGGGTG
TACGTGGCCGGAGCCCCCCGGTTCAACCACACGGGCAAGGTCATCCTGTTCACCATGCAC
AACAACCGGAGCCTCACCATCCACCAGGCTATGCGGGGCCAGCAGATAGGCTCTTACTTT
GGGAGTGAAATCACCTCGGTGGACATCGACGGCGACGGCGTGACTGATGTCCTGCTGGTG
GGCGCACCCATGTACTTCAACGAGGGCCGTGAGCGAGGCAAGGTGTACGTCTATGAGCTG
AGACAGAACCTGTTTGTTTATAACGGAACGCTAAAGGATTCACACAGTTACCAGAATGCC
CGATTTGGGTCCTCCATTGCCTCAGTTCGAGACCTCAACCAGGATTCCTACAATGACGTG
GTGGTGGGAGCCCCCCTGGAGGACAACCACGCAGGAGCCATCTACATCTTCCACGGCTTC
CGAGGCAGCATCCTGAAGACACCTAAGCAGAGAATCACAGCCTCAGAGCTGGCTACCGGC
CTCCAGTATTTTGGCTGCAGCATCCACGGGCAATTGGACCTCAATGAGGATGGGCTCATC
GACCTGGCAGTGGGAGCCCTTGGCAACGCTGTGATTCTGTGGTCCCGCCCAGTGGTTCAG
ATCAATGCCAGCCTCCACTTTGAGCCATCCAAGATCAACATCTTCCACAGAGACTGCAAG
CGCAGTGGCAGGGATGCCACCTGCCTGGCCGCCTTCCTCTGCTTCACGCCCATCTTCCTG
GCACCCCATTTCCAAACAACAACTGTTGGCATCAGATACAACGCCACCATGGATGAGAGG
CGGTATACACCGAGGGCCCACCTGGACGAGGGCGGGGACCGATTCACCAACAGAGCCGTA
CTGCTCTCCTCCGGCCAGGAGCTCTGTGAGCGGATCAACTTCCATGTCCTGGACACTGCT
GACTACGTGAAGCCAGTGACCTTCTCAGTCGAGTATTCCCTGGAGGACCCTGACCATGGC
CCCATGCTGGACGACGGCTGGCCCACCACTCTCAGAGTCTCGGTGCCCTTCTGGAACGGC
TGCAATGAGGATGAGCACTGTGTCCCTGACCTTGTGTTGGATGCCCGGAGTGACCTGCCC
ACGGCCATGGAGTACTGCCAGAGGGTGCTGAGGAAGCCTGCGCAGGACTGCTCCGCATAC
ACGCTGTCCTTCGACACCACAGTCTTCATCATAGAGAGCACACGCCAGCGAGTGGCGGTG
GAGGCCACACTGGAGAACAGGGGCGAGAACGCCTACAGCACGGTCCTAAATATCTCGCAG
TCAGCAAACCTGCAGTTTGCCAGCTTGATCCAGAAGGAGGACTCAGACGGTAGCATTGAG
TGTGTGAACGAGGAGAGGAGGCTCCAGAAGCAAGTCTGCAACGTCAGCTATCCCTTCTTC
CGGGCCAAGGCCAAGGTGGCTTTCCGTCTTGATTTTGAGTTCAGCAAATCCATCTTCCTA
CACCACCTGGAGATCGAGCTCGCTGCAGGCAGTGACAGTAATGAGCGGGACAGCACCAAG
GAAGACAACGTGGCCCCCTTACGCTTCCACCTCAAATACGAGGCTGACGTCCTCTTCACC
AGGAGCAGCAGCCTGAGCCACTACGAGGTCAAGCCCAACAGCTCGCTGGAGAGATACGAT
GGTATCGGGCCTCCCTTCAGCTGCATCTTCAGGATCCAGAACTTGGGCTTGTTCCCCATC
CACGGGATGATGATGAAGATCACCATTCCCATCGCCACCAGGAGCGGCAACCGCCTACTG
AAGCTGAGGGACTTCCTCACGGACGAGGCGAACACGTCCTGTAACATCTGGGGCAATAGC
ACTGAGTACCGGCCCACCCCAGTGGAGGAAGACTTGCGTCGTGCTCCACAGCTGAATCAC
AGCAACTCTGATGTCGTCTCCATCAACTGCAATATACGGCTGGTCCCCAACCAGGAAATC
AATTTCCATCTACTGGGGAACCTGTGGTTGAGGTCCCTAAAAGCACTCAAGTACAAATCC
ATGAAAATCATGGTCAACGCAGCCTTGCAGAGGCAGTTCCACAGCCCCTTCATCTTCCGT
GAGGAGGATCCCAGCCGCCAGATCGTGTTTGAGATCTCCAAGCAAGAGGACTGGCAGGTC
CCCATCTGGATCATTGTAGGCAGCACCCTGGGGGGCCTCCTACTGCTGGCCCTGCTGGTC
CTGGCACTGTGGAAGCTCGGCTTCTTTAGAAGTGCCAGGCGCAGGAGGGAGCCTGGTCTG
GACCCCACCCCCAAAGTGCTGGAG
Protein sequence predicted based on nucleotide sequence
MDLPRGLVVAWALSLWPGFTDTFNMDTRKPRVIPGSRTAFFGYTVQQHDISGNKWLVVGA PLETNGYQKTGDVYKCPVIHGNCTKLNLGRVTLSNVSERKDNMRLGLSLATNPKDNSFLA CSPLWSHECGSSYYTTGMCSRVNSNFRFSKTVAPALQRCQTYMDIVIVLDGSNSIYPWVE VQHFLINILKKFYIGPGQIQVGVVQYGEDVVHEFHLNDYRSVKDVVEAASHIEQRGGTET RTAFGIEFARSEAFQKGGRKGAKKVMIVITDGESHDSPDLEKVIQQSERDNVTRYAVAVL GYYNRRGINPETFLNEIKYIASDPDDKHFFNVTDEAALKDIVDALGDRIFSLEGTNKNET SFGLEMSQTGFSSHVVEDGVLLGAVGAYDWNGAVLKETSAGKVIPLRESYLKEFPEELKN HGAYLGYTVTSVVSSRQGRVYVAGAPRFNHTGKVILFTMHNNRSLTIHQAMRGQQIGSYF GSEITSVDIDGDGVTDVLLVGAPMYFNEGRERGKVYVYELRQNLFVYNGTLKDSHSYQNA RFGSSIASVRDLNQDSYNDVVVGAPLEDNHAGAIYIFHGFRGSILKTPKQRITASELATG LQYFGCSIHGQLDLNEDGLIDLAVGALGNAVILWSRPVVQINASLHFEPSKINIFHRDCK RSGRDATCLAAFLCFTPIFLAPHFQTTTVGIRYNATMDERRYTPRAHLDEGGDRFTNRAV LLSSGQELCERINFHVLDTADYVKPVTFSVEYSLEDPDHGPMLDDGWPTTLRVSVPFWNG CNEDEHCVPDLVLDARSDLPTAMEYCQRVLRKPAQDCSAYTLSFDTTVFIIESTRQRVAV EATLENRGENAYSTVLNISQSANLQFASLIQKEDSDGSIECVNEERRLQKQVCNVSYPFF RAKAKVAFRLDFEFSKSIFLHHLEIELAAGSDSNERDSTKEDNVAPLRFHLKYEADVLFT RSSSLSHYEVKPNSSLERYDGIGPPFSCIFRIQNLGLFPIHGMMMKITIPIATRSGNRLL KLRDFLTDEANTSCNIWGNSTEYRPTPVEEDLRRAPQLNHSNSDVVSINCNIRLVPNQEI NFHLLGNLWLRSLKALKYKSMKIMVNAALQRQFHSPFIFREEDPSRQIVFEISKQEDWQV PIWIIVGSTLGGLLLLALLVLALWKLGFFRSARRRREPGLDPTPKVLE
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
3654
Start on reference sequence
88
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
