Gene:
Gene Symbol:
RIMBP3C
Gene Name:
RIMS binding protein 3C
Original Clone ID:
FLH481407.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00295152
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGCCAAGGACTCGCCCAGCCCCTTGGGCGCGTCGCCCAAGAAGCCGGGCTGCTCCAGC
CCGGCGGCGGCAGTGCTGGAGAACCAGAGGCGGGAGCTGGAGAAGCTACGGGCGGAGCTG
GAGGCGGAGCGGGCAGGCTGGCGGGCGGAACGGCGGCGCTTCGCTGCCCGGGAGCGCCAG
CTGCGTGAGGAGGCCGAGCGGGAGCGGCGACAGCTGGCTGACCGTCTGCGCTCCAAGTGG
GAGGCACAGCGCAGCCGGGAGTTGCGGCAGCTGCAAGAGGAGATGCAGCGGGAACGCGAG
GCCGAGATCCGGCAGCTGCTGCGCTGGAAGGAGGCCGAGCAGCGGCAGCTGCAGCAGCTG
CTGCACCGGGAGCGCGATGGCGTGGTGCGCCAAGCCCGGGAGCTGCAGCGCCAGCTGGCC
GAGGAGCTGGTGAACCGCGGCCACTGTAGCCGCCCGGGGGCGTCCGAGGTTTCCGCGGCG
CAGTGCCGCTGTCGCCTGCAGGAAGTGTTGGCGCAGCTTCGCTGGCAGACTGACGGCGAG
CAGGCGGCGCGCATCCGCTATCTGCAGGCGGCGCTGGAGGTGGAGCGCCAGCTCTTCCTC
AAGTACATCCTGGCGCACTTCCGCGGGCACCCGGCTTTGTCGGGATCACCGGACCCCCAA
GCTGTGCATTCCTTGGAAGAACCGCTGCCCCAGACCTCCAGCGGCTCTTGCCACGCCCCC
AAACCCGCCTGCCAACTCGGATCTCTAGACAGCCTGAGTGCTGAAGTCGGTGTGCGCTCC
CGCTCGCTAGGCCTGGTGTCCTCTGCGTGCTCCAGCTCCCCAGACGGCCTGCTCTCCACG
CACGCCAGCTCCCTTGATTGCTTCGCACCTGCGTGTTCCCGCTCGCTTGACAGCACCCGG
AGCCTCCCCAAGGCCTCCAAATCCGAGGAGCGGCCCTCCTCACCAGACACCTCCACCCCT
GGCTCCCGGAGGCTCTCGCCGCCACCATCGCCACTCCCGCCGCCACCACCACCGTCAGCC
CACAGGAAACTCAGCAACCCGCGGGGAGGAGAAGGCTCTGAGAGCCAGCCCTGCGAAGTC
CTGACTCCCTCACCCCCGGGCCTGGGCCACCACGAGCTGATAAAGCTGAACTGGCTGCTG
GCCAAGGCGTTGTGGGTGCTGGCGCGCCGCTGTTATACCCTGCAAGCGGAGAACAAGCAG
CTGCGGCGTGCAGGCTGCCCCTACCAGGCAGACGAGAAGGTGAAGCGGCTCAAGGTAAAG
CGCGCGGAGCTGACCGGGCTCGCGCGGCGCCTAGCTGACCGCGCCCGCAAGCTGCAGGAG
ACCAACCTCCGGGCCGTGAGCGCGCCTATACCCGGCGAGAGTTGCGCCGGCCTGGAGCTG
TGCCAAGTCTTTGCCCGCCAGCGCGCTCGGGACCTGTCGGAGCAGGCGAGCGCGCCGCTG
GCCAAGGACAAGCAGATCGAAGAGCTGCGGCAGGAGTGCCACCTCCTGCAGGCGCGTGTC
GCCTCGGGTCCCTGCAGCGACCTGCATACTGGAAGGGGCGGCCCCTGCACCCAGTGGCTC
AACGTCAGAGACTTAGACCGCCTGCAGCGCGAGTCCCAGCGGGAAGTGCTGCGCCTGCAG
AGGCAGTTGATGCTTCAGCAGGGCAACGGTGGCGCTTGGCCCGAGGCGGGCGGCCAGAGC
GCAACCTGCGAGGAGGTGCGACGGCAGATGCTGGCGCTGGAGCGCGAGCTGGACCAGCGG
CGGCGCGAGTGCCAGGAGCTGGGCGCGCAGGCGGCCCCGGCGCGGCGACGTGGCGAGGAG
GCCGAGACACAGCTGCAGGCGGCGCTGCTCAAAAACGCCTGGCTGGCGGAGGAGAATGGG
CGGCTGCAGGCCAAGACCGACTGGGTGCGGAAGGTGGAGGCTGAGAATAGCGAAGTGCGC
GGCCACCTGGGCCGCGCGTGTCAAGAGCGCGATGCCTCCGGCTTGATCGCCGAACAGCTG
CTGCAGCAGGCGGCGCGCGGGCAGGACAGGCAGCAGCAGCTGCAACGCGACCCGCAGAAG
GCCCTGTGTGACCTCCATCCTTCCTGGAAGGAGATACAGGCGCTCCAGTGTCGGCCTGGT
CACCCTCCTGAACAGCCCTGGGAGACCAGTCAAATGCCGGAGTCCCAAGTTAAAGGTAGC
AGAAGGCCCAAGTTCCACGCACGGCCTGAAGACTACGCAGTGTCACAGCCCAACAGAGAC
ATACAGGAGAAAAGGGAAGCCTCCCTCGAGGAGAGCCCAGTTGCCCTTGGGGAGTCAGCC
AGTGTCCCCCAAGTTTCAGAGACAGTCCCTGCCAGCCAACCTCTGTCCAAGAAAACCAGC
TCCCAGTCAAACTCCTCCTCTGAGGGGTCGATGTGGGCCACCGTGCCGTCCTCCCCTACT
CTGGACAGGGACACAGCCAGTGAGGTGGATGACCTGGAGCCTGACAGCGTGTCCCTGGCC
CTGGAAATGGGGGGCTCGGCGGCTCCTGCTGCCCCCAAGCTCAAGATCTTCATGGCTCAG
TATAACTACAACCCATTTGAGGGGCCCAATGATCACCCTGAGGGTGAGCTGCCCCTCACA
GCTGGGGACTACATATATATCTTCGGGGACATGGATGAGGATGGCTTCTATGAGGGGGAG
CTTGAGGATGGCCGGCGGGGGCTGGTGCCCTCCAACTTCGTGGAGCAGATTCCGGACAGC
TACATCCCAGGCTGCCTGCCTGCCAAATCCCCTGATCTTGGCCCCAGTCAACTCCCAGCG
GGGCAGGATGAAGCTCTGGAGGAAGACAGCTTATTATCTGGGAAAGCCCAGGGAGTGGTG
GACAGAGGGCTGTGCCAGATGGTCAGGGTGGGCTCCAAGACAGAAGTAGCAACAGAGATC
CTGGATACCAAGACGGAAGCCTGCCAGCTGGGCTTGCTGCAGAGCATGGGGAAGCAGGGC
CTCTCCAGACCCCTTCTGGGGACCAAAGGGGTGCTCCGTATGGCTCCCATGCAGCTACAC
CTGCAGAATGTCACAGCCACATCAGCCAACATCACCTGGGTCTACAGCAGCCACCGCCAC
CCCCATGTGGTATATCTTGATGACCGAGAGCATGCCCTGACCCCAGCGGGCGTGAGCTGC
TACACCTTCCAGGGCCTGTGCCCCGGCACGCACTACCGGGCGCGGGTGGAGGTGCGGCTG
CCACGGGACTTGCTGCAGGTGTATTGGGGAACTATGTCCTCCACCGTCACCTTCGACACA
CTCTTGGCAGGACCTCCCTACCCACCGCTGGATGTGCTGGTGGAGCGCCATGCCTCGCCA
GGTGTCCTGGTGGTCAGCTGGCTCCCTGTGACCATTGACTCAGCTGGGTCCTCCAATGGA
GTCCAGGTCACCGGTTATGCTGTGTATGCAGATGGGCTTAAGGTTTGTGAGGTCGCCGAT
GCCACTGCTGGGAGCACCCTATTGGAATTCTCCCAGCTACAGGTGCCCCTCACGTGGCAG
AAGGTCTCAGTGAGAACCATGTCACTCTGTGGTGAGTCCCTGGATTCAGTGCCTGCTCAG
ATCCCCGAGGACTTCTTCATGTGTCACCGATGGCCAGAGACTCCACCCTTTAGCTACACT
TGTGGCGACCCATCCACCTACAGAGTCACCTTCCCCGTCTGCCCCCAGAAGCTGTCACTG
GCTCCTCCGAGTGCCAAGGCCAGCCCCCACAACCCTGGAAGCTGCGGGGAGCCCCAGGCC
AAGTTCCTAGAAGCATTCTTTGAAGAACCCCCAAGGAGGCAATCCCCAGTGTCCAACCTG
GGCTCAGAAGGAGAATGTCCGAGTTCAGGGGCTGGCAGCCAAGCCCAGGAGCTTGCAGAG
GCCTGGGAGGGCTGTAGAAAGGACCTGCTCTTTCAGAAGAGTCCCCAGAACCACAGGCCA
CCTTCAGTCAGTGACCAGCCTGGGGAGAAGGAAAATTGCTCCCAGCACATGGGCACCAGC
AAAAGCCCTGCTCCAGGATTCATCCATCTACGCACCGAGTGTGGGCCCAGGAAAGAACCG
TGTCAGGAAAAGGCTGCCCTTGAGAGGGTACTTCGGCAAAAGCAAGATGCCCAAGGGTTC
ACACCTCCCCAGCTGGGCGCCAGCCAACAGTATGCATCTGACTTCCATAACGTTTTGAAG
GAGGAGCAGGAGGCACTGTGCTTGGATCTGTGGGGCACAGAGAGGCGAGAGGAGAGGAGG
GAGCCTGAGCCCCACAGCAGGCAAGGACAAGCTCTGGGGGTGAAGAGAGGGTGCCAGCTC
CATGAGCCCAGCTCGGCACTGTGTCCAGCTCCATCCGCCAAAGTCATCAAGATGCCCAGG
GGTGGCCCCCAACAGCTGGGGACGGGGGCCAACACTCCAGCCAGGGTCTTTGTGGCCCTC
TCTGATTACAACCCCCTGGTGATGTCTGCCAACCTCAAGGCTGCAGAGGAGGAGCTGGTC
TTCCAGAAAAGGCAGTTGCTAAGAGTGTGGGGCTCTCAGGACACCCATGATTTCTACCTC
AGCGAGTGCAACAGGCAAGTGGGCAATATCCCCGGGCGCCTAGTGGCTGAGATGGAGGTG
GGGACAGAGCAGACTGATAGGAGGTGGCGTTCTCCGGCCCAAGGGAACCTGCCTTCTGTG
GCCCACCTCGAGGACTTTCAGGGGCTCACCATCCCCCAGGGTTCCTCCCTGGTGCTCCAG
GGGAACTCCAAGAGACTCCCACTGTGGACTCCAAAGATCATGATAGCAGCTCTGGACTAT
GATCCTGGGGATGGGCAAATGGGGGGCCAGGGGAAGGGCAGGCTGGCGCTGAGGGCAGGA
GACGTGGTCATGGTTTACGGGCCCATGGATGACCAAGGATTCTATTATGGAGAGTTGGGC
GGCCACAGGGGCCTGGTTCCTGCCCACCTGCTGGATCACATGTCCCTCCATGGACAC
Protein sequence predicted based on nucleotide sequence
MAKDSPSPLGASPKKPGCSSPAAAVLENQRRELEKLRAELEAERAGWRAERRRFAARERQ LREEAERERRQLADRLRSKWEAQRSRELRQLQEEMQREREAEIRQLLRWKEAEQRQLQQL LHRERDGVVRQARELQRQLAEELVNRGHCSRPGASEVSAAQCRCRLQEVLAQLRWQTDGE QAARIRYLQAALEVERQLFLKYILAHFRGHPALSGSPDPQAVHSLEEPLPQTSSGSCHAP KPACQLGSLDSLSAEVGVRSRSLGLVSSACSSSPDGLLSTHASSLDCFAPACSRSLDSTR SLPKASKSEERPSSPDTSTPGSRRLSPPPSPLPPPPPPSAHRKLSNPRGGEGSESQPCEV LTPSPPGLGHHELIKLNWLLAKALWVLARRCYTLQAENKQLRRAGCPYQADEKVKRLKVK RAELTGLARRLADRARKLQETNLRAVSAPIPGESCAGLELCQVFARQRARDLSEQASAPL AKDKQIEELRQECHLLQARVASGPCSDLHTGRGGPCTQWLNVRDLDRLQRESQREVLRLQ RQLMLQQGNGGAWPEAGGQSATCEEVRRQMLALERELDQRRRECQELGAQAAPARRRGEE AETQLQAALLKNAWLAEENGRLQAKTDWVRKVEAENSEVRGHLGRACQERDASGLIAEQL LQQAARGQDRQQQLQRDPQKALCDLHPSWKEIQALQCRPGHPPEQPWETSQMPESQVKGS RRPKFHARPEDYAVSQPNRDIQEKREASLEESPVALGESASVPQVSETVPASQPLSKKTS SQSNSSSEGSMWATVPSSPTLDRDTASEVDDLEPDSVSLALEMGGSAAPAAPKLKIFMAQ YNYNPFEGPNDHPEGELPLTAGDYIYIFGDMDEDGFYEGELEDGRRGLVPSNFVEQIPDS YIPGCLPAKSPDLGPSQLPAGQDEALEEDSLLSGKAQGVVDRGLCQMVRVGSKTEVATEI LDTKTEACQLGLLQSMGKQGLSRPLLGTKGVLRMAPMQLHLQNVTATSANITWVYSSHRH PHVVYLDDREHALTPAGVSCYTFQGLCPGTHYRARVEVRLPRDLLQVYWGTMSSTVTFDT LLAGPPYPPLDVLVERHASPGVLVVSWLPVTIDSAGSSNGVQVTGYAVYADGLKVCEVAD ATAGSTLLEFSQLQVPLTWQKVSVRTMSLCGESLDSVPAQIPEDFFMCHRWPETPPFSYT CGDPSTYRVTFPVCPQKLSLAPPSAKASPHNPGSCGEPQAKFLEAFFEEPPRRQSPVSNL GSEGECPSSGAGSQAQELAEAWEGCRKDLLFQKSPQNHRPPSVSDQPGEKENCSQHMGTS KSPAPGFIHLRTECGPRKEPCQEKAALERVLRQKQDAQGFTPPQLGASQQYASDFHNVLK EEQEALCLDLWGTERREERREPEPHSRQGQALGVKRGCQLHEPSSALCPAPSAKVIKMPR GGPQQLGTGANTPARVFVALSDYNPLVMSANLKAAEEELVFQKRQLLRVWGSQDTHDFYL SECNRQVGNIPGRLVAEMEVGTEQTDRRWRSPAQGNLPSVAHLEDFQGLTIPQGSSLVLQ GNSKRLPLWTPKIMIAALDYDPGDGQMGGQGKGRLALRAGDVVMVYGPMDDQGFYYGELG GHRGLVPAHLLDHMSLHGH
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
486
End on reference sequence
5405
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
