Gene:
Gene Symbol:
LEMD3
Gene Name:
LEM domain containing 3
Original Clone ID:
FLH481416.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00295161
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGCGGCGGCAGCAGCTTCGGCGCCTCAGCAGCTCTCGGATGAGGAGCTTTTCTCTCAG
CTCCGCCGTTACGGCCTGTCTCCCGGACCAGTGACGGAGAGCACCCGCCCGGTCTACCTC
AAGAAGCTGAAGAAGCTTCGAGAGGAAGAGCAGCAACAGCACCGGTCAGGGGGCCGCGGC
AACAAGACGCGGAACAGTAATAACAATAACACGGCAGCCGCCACGGTCGCAGCCGCGGGA
CCAGCGGCGGCGGCGGCCGCGGGGATGGGGGTCCGGCCGGTCTCGGGCGACCTCTCCTAC
TTACGGACTCCTGGGGGCCTGTGCCGAATCTCGGCCTCTGGCCCAGAGAGCCTCCTGGGA
GGGCCCGGGGGCGCCTCCGCCGCCCCCGCGGCTGGCAGCAAAGTGCTGCTGGGCTTCAGC
TCGGACGAGTCGGACGTGGAGGCCAGTCCCCGGGACCAGGCCGGCGGCGGCGGGAGGAAA
GACCGGGCTTCGCTCCAGTACCGCGGGCTCAAAGCGCCGCCGGCGCCCCTGGCCGCCAGC
GAGGTGACTAACAGCAACTCTGCAGAGCGAAGGAAGCCCCACTCGTGGTGGGGGGCCAGG
AGGCCGGCGGGCCCCGAGCTGCAGACCCCGCCGGGGAAAGATGGAGCAGTGGAGGACGAG
GAAGGGGAGGGAGAGGACGGTGAGGAGAGGGACCCGGAGACCGAGGAGCCGCTCTGGGCG
AGCCGGACCGTGAATGGCAGCCGGCTTGTCCCCTACAGCTGCCGGGAAAACTATTCGGAC
TCAGAGGAAGAGGACGACGACGACGTGGCCTCCAGCAGACAGGTATTAAAGGACGACTCC
CTTTCCCGGCATCGGCCCAGACGAACCCATAGTAAGCCTCTCCCCCCGCTGACTGCTAAA
TCGGCCGGCGGCAGGCTGGAGACTTCAGTTCAGGGAGGGGGAGGACTCGCGATGAATGAC
AGGGCGGCGGCTGCCGGGAGTCTAGACAGGAGCCGAAACCTCGAAGAGGCGGCGGCCGCG
GAGCAGGGAGGAGGGTGTGATCAAGTGGACTCCAGCCCCGTTCCTAGATACCGTGTTAAC
GCTAAGAAACTGACCCCTCTCCTGCCCCCGCCACTTACTGACATGGACTCAACCTTGGAT
TCGTCAACAGGCTCCCTTCTGAAAACCAATAATCATATTGGCGGTGGGGCCTTCAGTGTG
GACTCCCCCAGGATTTATTCTAACAGTCTCCCTCCCAGTGCGGCGGTGGCCGCCTCTAGT
TCACTCAGGATCAATCACGCCAATCATACGGGCTCCAATCATACCTACCTGAAAAACACA
TACAACAAACCGAAGCTTTCCGAACCCGAAGAGGAACTTCTCCAGCAATTTAAACGGGAG
GAGGTGTCCCCAACAGGGAGTTTCAGTGCCCACTACTTGTCGATGTTTCTCTTAACTGCT
GCCTGCTTATTTTTCCTAATACTGGGACTGACTTACCTAGGAATGAGAGGGACAGGAGTA
TCTGAGGATGGAGAACTCAGCATAGAAAACCCCTTTGGTGAAACATTTGGAAAAATACAA
GAAAGTGAAAAAACTCTTATGATGAACACATTATATAAGCTTCATGATCGATTGGCACAG
CTTGCAGGAGATCATGAATGTGGCAGTTCTAGTCAAAGAACGCTTTCTGTTCAAGAGGCA
GCTGCGTATTTAAAAGATTTAGGTCCTGAATATGAAGGTATATTTAACACTTCATTGCAG
TGGATCTTAGAAAATGGAAAAGATGTTGGAATAAGGTGTGTTGGTTTTGGCCCTGAGGAA
GAATTGACAAATATAACTGATGTGCAGTTTTTACAGTCCACAAGACCACTGATGTCTTTT
TGGTGTCGTTTTCGACGTGCTTTTGTTACTGTAACTCACAGATTATTGTTGTTATGCTTA
GGTGTAGTGATGGTTTGTGTCGTTCTGCGTTACATGAAATATCGATGGACAAAAGAAGAG
GAGGAAACAAGGCAGATGTATGATATGGTGGTAAAGATTATAGATGTTTTACGAAGTCAT
AATGAAGCCTGCCAGGAAAACAAAGATTTACAACCTTACATGCCTATTCCACATGTACGC
GATTCCTTAATACAGCCTCATGACAGGAAAAAAATGAAGAAAGTCTGGGATAGAGCTGTT
GACTTCCTTGCTGCTAATGAGTCTAGAGTTCGCACGGAAACACGAAGAATAGGTGGTGCA
GATTTTCTGGTTTGGCGGTGGATCCAGCCTTCTGCATCCTGTGACAAAATATTAGTTATA
CCTTCTAAAGTATGGCAAGGTCAAGCATTTCATTTAGATAGAAGAAATTCACCACCAAAT
AGTTTGACACCGTGTCTAAAGATTCGGAATATGTTTGATCCCGTTATGGAAATAGGGGAT
CAGTGGCATTTGGCAATTCAAGAAGCAATTTTAGAAAAATGCAGTGATAATGATGGCATT
GTTCACATTGCAGTAGACAAAAATTCACGTGAGGGTTGTGTATATGTTAAATGTCTGTCT
CCAGAATATGCTGGAAAGGCTTTTAAAGCATTGCATGGCTCTTGGTTTGATGGGAAATTG
GTTACAGTAAAATATTTACGACTAGATAGATACCACCATCGCTTTCCCCAGGCTCTCACT
TCCAACACTCCATTGAAGCCATCAAATAAACATATGAACTCCATGTCTCATCTTCGTCTT
CGGACTGGCCTAACCAATTCTCAAGGAAGTTCC
Protein sequence predicted based on nucleotide sequence
MAAAAASAPQQLSDEELFSQLRRYGLSPGPVTESTRPVYLKKLKKLREEEQQQHRSGGRG NKTRNSNNNNTAAATVAAAGPAAAAAAGMGVRPVSGDLSYLRTPGGLCRISASGPESLLG GPGGASAAPAAGSKVLLGFSSDESDVEASPRDQAGGGGRKDRASLQYRGLKAPPAPLAAS EVTNSNSAERRKPHSWWGARRPAGPELQTPPGKDGAVEDEEGEGEDGEERDPETEEPLWA SRTVNGSRLVPYSCRENYSDSEEEDDDDVASSRQVLKDDSLSRHRPRRTHSKPLPPLTAK SAGGRLETSVQGGGGLAMNDRAAAAGSLDRSRNLEEAAAAEQGGGCDQVDSSPVPRYRVN AKKLTPLLPPPLTDMDSTLDSSTGSLLKTNNHIGGGAFSVDSPRIYSNSLPPSAAVAASS SLRINHANHTGSNHTYLKNTYNKPKLSEPEEELLQQFKREEVSPTGSFSAHYLSMFLLTA ACLFFLILGLTYLGMRGTGVSEDGELSIENPFGETFGKIQESEKTLMMNTLYKLHDRLAQ LAGDHECGSSSQRTLSVQEAAAYLKDLGPEYEGIFNTSLQWILENGKDVGIRCVGFGPEE ELTNITDVQFLQSTRPLMSFWCRFRRAFVTVTHRLLLLCLGVVMVCVVLRYMKYRWTKEE EETRQMYDMVVKIIDVLRSHNEACQENKDLQPYMPIPHVRDSLIQPHDRKKMKKVWDRAV DFLAANESRVRTETRRIGGADFLVWRWIQPSASCDKILVIPSKVWQGQAFHLDRRNSPPN SLTPCLKIRNMFDPVMEIGDQWHLAIQEAILEKCSDNDGIVHIAVDKNSREGCVYVKCLS PEYAGKAFKALHGSWFDGKLVTVKYLRLDRYHHRFPQALTSNTPLKPSNKHMNSMSHLRL RTGLTNSQGSS
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
2742
Start on reference sequence
7
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
