Gene:
Gene Symbol:
LNPEP
Gene Name:
leucyl/cystinyl aminopeptidase
Original Clone ID:
FLH481428.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00295173
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGGAGCCCTTCACCAATGATCGGCT
TCAGCTCCCCAGGAATATGATTGAAAACAGCATGTTTGAGGAAGAACCAGATGTGGTGGA
TTTAGCCAAAGAGCCTTGTTTACATCCTCTAGAGCCTGATGAGGTGGAATATGAGCCCCG
GGGTTCCCGACTGCTGGTGCGGGGTCTTGGTGAGCATGAGATGGAGGAGGATGAAGAGGA
TTATGAGTCATCAGCAAAGCTGCTGGGCATGTCCTTCATGAATAGAAGCTCAGGCCTTCG
GAACAGTGCAACTGGTTACAGGCAGAGCCCAGATGGGGCTTGTTCAGTACCCTCTGCAAG
GACCATGGTGGTCTGTGCTTTTGTCATCGTGGTTGCTGTTTCTGTAATCATGGTGATTTA
CTTACTGCCCAGATGTACCTTTACCAAAGAAGGCTGCCATAAAAAAAACCAGTCAATTGG
ACTAATTCAGCCATTTGCAACAAATGGGAAATTGTTTCCATGGGCACAGATCAGGCTTCC
CACTGCCGTTGTGCCACTACGCTATGAACTCAGCCTACACCCGAACCTAACCTCGATGAC
ATTCAGGGGTTCTGTGACAATTTCAGTTCAGGCTCTTCAGGTCACATGGAATATCATTCT
TCATAGCACAGGTCATAATATTTCAAGAGTGACCTTTATGTCAGCAGTTTCAAGCCAAGA
AAAACAAGCTGAGATCCTGGAATATGCATATCATGGACAGATCGCCATTGTTGCCCCCGA
AGCCCTTCTAGCAGGGCACAATTATACGTTGAAGATAGAGTACTCGGCAAATATATCTAG
TTCTTATTATGGGTTTTATGGCTTCTCCTACACAGATGAAAGTAATGAGAAAAAGTACTT
TGCAGCAACTCAGTTTGAACCCCTGGCAGCAAGATCTGCTTTTCCTTGTTTTGATGAACC
AGCATTTAAAGCCACTTTTATCATCAAGATCATAAGGGATGAGCAATACACCGCTTTATC
AAATATGCCTAAGAAGTCATCAGTCGTTCTAGATGATGGACTTGTTCAGGATGAGTTTTC
TGAGAGTGTGAAGATGAGCACTTACTTGGTTGCTTTCATTGTGGGAGAGATGAAGAACCT
GAGTCAGGACGTAAATGGAACCCTGGTTTCTATATATGCTGTACCAGAAAAGATTGGTCA
AGTTCATTATGCCTTGGAAACAACTGTGAAGCTTCTTGAGTTTTTTCAAAACTACTTTGA
AATTCAGTACCCACTTAAGAAATTGGATTTGGTGGCTATTCCTGACTTTGAAGCAGGAGC
AATGGAAAATTGGGGTTTGCTCACCTTCCGAGAGGAGACACTTCTGTATGACAGTAACAC
TTCTTCAATGGCGGATAGAAAGCTGGTGACTAAAATCATTGCTCATGAGCTGGCCCACCA
GTGGTTTGGCAATCTGGTAACAATGAAGTGGTGGAATGACCTATGGCTAAATGAAGGTTT
TGCCACTTTCATGGAGTATTTCTCTTTGGAAAAAATATTCAAAGAGCTTTCTAGTTATGA
AGATTTCTTAGATGCTCGATTTAAAACCATGAAGAAAGATTCCTTAAATTCATCTCATCC
AATATCATCATCTGTTCAGTCTTCAGAACAAATTGAAGAAATGTTTGATTCTCTTTCCTA
TTTTAAGGGATCTTCTCTCTTGTTGATGTTGAAAACTTACCTTAGTGAAGATGTGTTTCA
ACATGCTGTTGTCCTTTACCTGCATAATCACAGCTATGCATCTATTCAAAGTGATGATCT
GTGGGATAGTTTTAATGAGGTCACAAACCAAACACTAGATGTAAAGAGAATGATGAAAAC
CTGGACCCTGCAGAAAGGATTTCCTTTAGTGACTGTTCAAAAGAAAGGAAAGGAACTTTT
TATACAACAAGAGAGATTCTTTTTAAATATGAAGCCTGAAATTCAGCCTTCAGATACAAG
CTACCTGTGGCATATTCCACTATCCTATGTCACTGAAGGAAGAAATTATTCAAAATATCA
ATCGGTATCATTACTGGATAAGAAATCAGGTGTCATCAATCTTACAGAAGAAGTGCTGTG
GGTCAAAGTGAATATAAACATGAATGGTTATTATATTGTACACTATGCAGATGATGATTG
GGAAGCACTAATCCATCAGTTGAAAATAAATCCTTATGTTCTGAGTGACAAAGACCGAGC
CAACCTTATCAACAACATCTTTGAACTTGCAGGCCTAGGCAAGGTACCTCTCAAGAGGGC
CTTTGATTTGATTAATTATCTTGGAAATGAGAACCATACTGCACCCATCACCGAAGCCCT
GTTTCAGACAGACCTCATCTATAACCTCCTTGAAAAACTGGGATACATGGATCTGGCCTC
AAGACTGGTGACTAGGGTATTTAAATTACTTCAAAACCAAATTCAACAACAAACTTGGAC
TGATGAGGGCACTCCATCTATGCGAGAGCTTCGGTCAGCCCTGCTAGAGTTTGCTTGCAC
CCACAACCTGGGGAACTGCTCTACTACTGCCATGAAACTGTTTGATGACTGGATGGCATC
CAATGGAACTCAAAGCCTACCTACTGATGTCATGACAACTGTGTTCAAAGTTGGAGCAAA
AACTGACAAAGGCTGGTCATTCCTTTTGGGCAAATACATTTCTATAGGCTCTGAAGCAGA
GAAGAACAAAATACTAGAAGCACTTGCCAGCTCAGAGGATGTGCGGAAGCTTTACTGGTT
AATGAAAAGTAGCCTGAATGGAGATAACTTCCGAACACAGAAGCTGTCTTTTATCATTAG
AACAGTGGGTCGACATTTTCCTGGACACTTACTGGCATGGGATTTTGTCAAAGAGAACTG
GAATAAGCTTGTACAGAAGTTCCCTCTGGGGTCCTATACCATACAAAATATTGTTGCTGG
ATCAACTTACCTGTTTTCAACAAAGACACATTTATCTGAGGTTCAGGCATTCTTTGAAAA
TCAGTCAGAGGCAACCTTCCGGCTTCGTTGTGTCCAGGAGGCTTTGGAAGTCATTCAGTT
GAATATCCAGTGGATGGAGAAGAACCTCAAAAGTCTCACATGGTGGCTGTAGGGCCTCAT
GGGCCCAGCTTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MEPFTNDRLQLPRNMIENSMFEEEPDVVDLAKEPCLHPLEPDEVEYEPRGSRLLVRGLGE HEMEEDEEDYESSAKLLGMSFMNRSSGLRNSATGYRQSPDGACSVPSARTMVVCAFVIVV AVSVIMVIYLLPRCTFTKEGCHKKNQSIGLIQPFATNGKLFPWAQIRLPTAVVPLRYELS LHPNLTSMTFRGSVTISVQALQVTWNIILHSTGHNISRVTFMSAVSSQEKQAEILEYAYH GQIAIVAPEALLAGHNYTLKIEYSANISSSYYGFYGFSYTDESNEKKYFAATQFEPLAAR SAFPCFDEPAFKATFIIKIIRDEQYTALSNMPKKSSVVLDDGLVQDEFSESVKMSTYLVA FIVGEMKNLSQDVNGTLVSIYAVPEKIGQVHYALETTVKLLEFFQNYFEIQYPLKKLDLV AIPDFEAGAMENWGLLTFREETLLYDSNTSSMADRKLVTKIIAHELAHQWFGNLVTMKWW NDLWLNEGFATFMEYFSLEKIFKELSSYEDFLDARFKTMKKDSLNSSHPISSSVQSSEQI EEMFDSLSYFKGSSLLLMLKTYLSEDVFQHAVVLYLHNHSYASIQSDDLWDSFNEVTNQT LDVKRMMKTWTLQKGFPLVTVQKKGKELFIQQERFFLNMKPEIQPSDTSYLWHIPLSYVT EGRNYSKYQSVSLLDKKSGVINLTEEVLWVKVNINMNGYYIVHYADDDWEALIHQLKINP YVLSDKDRANLINNIFELAGLGKVPLKRAFDLINYLGNENHTAPITEALFQTDLIYNLLE KLGYMDLASRLVTRVFKLLQNQIQQQTWTDEGTPSMRELRSALLEFACTHNLGNCSTTAM KLFDDWMASNGTQSLPTDVMTTVFKVGAKTDKGWSFLLGKYISIGSEAEKNKILEALASS EDVRKLYWLMKSSLNGDNFRTQKLSFIIRTVGRHFPGHLLAWDFVKENWNKLVQKFPLGS YTIQNIVAGSTYLFSTKTHLSEVQAFFENQSEATFRLRCVQEALEVIQLNIQWMEKNLKS LTWWL*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
3592
Start on reference sequence
515
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
