DNASU Plasmid | Zfp704 (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: Zfp704

Gene Name: zinc finger protein 704

Original Clone ID:

FLH481539.01X

Keyword:

None

Species:

Mus musculus

Type:

cDNA

Vector Name:

pENTR223.1

Format:

CLOSED

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

MmCD00295284

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1701nts

Open reading frame: 35 to 1735

GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGCAGGCCAGGCGCCTGGCCAAGCG
CCCCAGCCTGGGCAGCCGCCGCGGGGGCGCTGCGCCCGCTCCCGCCCCCGAGGCCGCCGC
TCTGGGGCTCCCGCCCCCCGGCCCCAGCCCGGCCGCCGCACCGGGAAGCTGGAGGCCGCC
GCTGCCGCCGCCGCGCGGGACCGGCCCCTCGCGCGCTGCCGCCGCCTCGTCGCCGGTCCT
GCTGCTGCTGGGCGAGGAGGACGAGGACGAGGAGGGCGCGGGCCGAAGGCGCAGGACGCG
CGGGCGGGTAACGGAGAAGCCCCGAGGGGTCGCGGAGGAGGAGGACGACGACGAGGAAGA
GGACGAAGAGGTGGTTGTCGAGGTGGTGGACGGCGACGAGGACGACGAGGACGCTGAGGA
GCGCTTCGTGCCCCTCGGACCCGGCCGTGCGCTTCCCAAGGGCCCCGCCCGAGGCGCGGT
GAAGGTGGGGAGTTTTAAGCGGGAGATGACCTTCACGTTTCAATCAGAGGACTTCAGACG
TGACTCCAGTAAGAAGCCGTCTCATCACCTGTTCCCACTGGCCATGGAGGAAGACGTGAG
AACAGCAGACACCAAAAAAACCAGTCGGGTCCTTGACCAAGAAAAAGAAACTCGGTCTGT
TTGTCTTCTTGAGCAGAAGCGGAAAGTAGTTTCTTCTAATATCGATGTCCCTCCTGCAAG
AAAATCTTCAGAGGAACTGGACATGGACAAGGTGACTGCAGCCATGGTATTGACCAGCTT
GTCAACCAGCCCTTTGGTCCGAAGCCCTCCTGTGCGGCCTAATGAGGGTCTCAGCGGGTC
CTGGAAGGAAGGTGCACCATCCAGCAGCAGCAGCAGTGGCTACTGGAGCTGGAGCGCTCC
GAGTGACCAGTCCAACCCATCCACGCCCTCTCCACCACTGTCAGCTGACAGCTTCAAGCC
CTTCCGCAGCCCCGCCCCACCTGACGATGGCATTGACGAGGCAGACGCCAGCAACCTGCT
CTTCGATGAGCCCATTCCGAGGAAGAGAAAGAACTCCATGAAGGTGATGTTCAAATGCCT
TTGGAAAAGCTGTGGCAAGGTGCTGAACACAGCTGCCGGCATCCAGAAGCACATCCGGGC
TGTCCACCTCGGGCGAGTTGGGGAGTCTGACTGCAGTGATGGGGAAGAAGACTTCTACTA
CACGGAGATCAAGCTCAACACGGATGCCACGGCTGAAGGCCTCAACACTGTGGCCCCGGT
TTCACCCTCCCAGTCTCTGGCTTCAGCCCCTGCGTTTCCCATCCCAGACTCCAGCCGAAC
AGAAACTCCTTGTGCCAAAACAGACACTAAGCTGGTGACGCCACTGAGCCGCTCAGCCCC
CACCACCCTCTACCTCGTGCACACTGACCATGCTTACCAGGCCACACCCCCGGTGACGAT
TCCTGGATCCGCTAAGTTCACCCCCAATGGCAGCAGCTTCAGCATTTCCTGGCAGTCTCC
CCCAGTCACTTTCACTGGCGTTCCGGTGTCTCCTCCACATCATCCCACAGCGGGCTCAGG
AGAGCAGAGACAGCACGCCCACACAGCCCTGTCCTCCCCGCCCAGAGGGACAGTCACCCT
AAGGAAGCCCAGAGGAGAAGGCAAAAAGTGCCGCAAGGTTTATGGGATGGAAAACAGGGA
CATGTGGTGCACCGCCTGCCGCTGGAAGAAGGCCTGCCAGCGGTTCATTGACTAGGGCCT
CATGGGCCCAGCTTTCTTGTAC

Protein sequence predicted based on nucleotide sequence

MQARRLAKRPSLGSRRGGAAPAPAPEAAALGLPPPGPSPAAAPGSWRPPLPPPRGTGPSR
AAAASSPVLLLLGEEDEDEEGAGRRRRTRGRVTEKPRGVAEEEDDDEEEDEEVVVEVVDG
DEDDEDAEERFVPLGPGRALPKGPARGAVKVGSFKREMTFTFQSEDFRRDSSKKPSHHLF
PLAMEEDVRTADTKKTSRVLDQEKETRSVCLLEQKRKVVSSNIDVPPARKSSEELDMDKV
TAAMVLTSLSTSPLVRSPPVRPNEGLSGSWKEGAPSSSSSSGYWSWSAPSDQSNPSTPSP
PLSADSFKPFRSPAPPDDGIDEADASNLLFDEPIPRKRKNSMKVMFKCLWKSCGKVLNTA
AGIQKHIRAVHLGRVGESDCSDGEEDFYYTEIKLNTDATAEGLNTVAPVSPSQSLASAPA
FPIPDSSRTETPCAKTDTKLVTPLSRSAPTTLYLVHTDHAYQATPPVTIPGSAKFTPNGS
SFSISWQSPPVTFTGVPVSPPHHPTAGSGEQRQHAHTALSSPPRGTVTLRKPRGEGKKCR
KVYGMENRDMWCTACRWKKACQRFID*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

End on reference sequence 2067

Start on reference sequence 367

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223.1

Synonyms:

pDONR-223.1, pENTR223-star-Sfi

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php

Size (bp):

4311

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

CTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGAAGGGCCGTCAAGGCCCTGCAGACTGGCTGTGTATAAGGGAGCCTGACATTTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGCGGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTGGCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCGATATGCACCACCGGGTAAAGTTCACGGGAGACTTTATCTGACAGCAGACGTGCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATCACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGTAAACCTTAAACTGCATTTCACCAGTCCCTGTTCTCGTCAGCAAAAGAGCCGTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCGCTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTGCTTACCAGACCGGAGATATTGACATCATATATGCCTTGAGCAACTGATAGCTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTCATAGCACACCTCTTTTTGACATACTTCGGGTATACATATCAGTATATATTCTTATACCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCTGGCTTTTAGTAAGCCGGATCCACGCGATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCGATCAACGTCTCATTTTCGCCAAAAGTTGGCCCAGGGCTTCCCGGTATCAACAGGGACAGGCCTCATGGGCCCAGCTTTCTTGTACAAAGTTGGCATTATAAAAAATAATTGCTCATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTGCCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCG

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (Sfi sites flank the cassette present only in no insert form)

1265

1277

bacterial origin

ori

bacterial origin of replication

534

negative selection marker

ccdB

ccdB (no insert form only; lost in with insert form)

1312

1617

primer site

M13 F

M13 forward primer for insert sequencing

1113

1129

primer site

T7 Forward primer for insert sequencing

2902

2921

primer site

M13 reverse

M13 reverse primer for insert sequencing

2926

2942

recombination site

attL1

attL1 recombination site

1165

1264

recombination site

attL2

attL2 recombination site

2786

2885

selectable marker

CmR

chlR (no insert form only; lost in with insert form)

1959

2618

selectable marker

SpnR

spectinomycin resistance gene

3154

4164

Gene

Gene Symbol :

Zfp704

Symbol Nomenclature :

Zfp704

SYNONYM :

C030026M03; C030034L19Rik; Gig1; Znf704

Designation :

glucocorticoid induced gene 1|glucocorticoid-induced gene 1 protein

Full Nomenclature :

zinc finger protein 704

GENEID :

170753

GI :

182887946

GenBank Accession :

BC160219

Mouse Genome Informatics :

2180715

Target GenBank:

NM_133218.1

Protein

SMART domain :

ZnF_C2H2 : zinc finger

UniProt :

Q9ERQ3

Clone

Cloning Information :

481539

HIP Master Clone ID :

399267

IMAGE ID :

100063867

Original Clone ID :

FLH481539.01X

Organism

Species Specific ID:

170753

Genome

Chromosome :

Map Location :

3 A1|3

Ensembl :

ENSMUSG00000040209

Reagents

Labome :

Zfp704-antibody