DNASU Plasmid | Fbxw9 (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: Fbxw9

Gene Name: F-box and WD-40 domain protein 9

Original Clone ID:

FLH481582.01X

Keyword:

None

Species:

Mus musculus

Type:

cDNA

Vector Name:

pENTR223.1

Format:

CLOSED

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

MmCD00295327

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1377nts

Open reading frame: 35 to 1411

GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGGATCTTTCCTCAGGGCGGAGTGG
GGATCCTCGCTCTTGTGAGGAGGAATCTGACCCCGAGCCAGATCCCGACCCTGACACTCA
AGCTGAGGCCTACGTAGCCCGTGTGCTTACCCCTCCCAAGCTTGGCCTGACCCCGCGGCG
CTCGTCACTGCAGTCCATGTTCTCCGCGTCCCTAGGCGTGCCGGAGCGAAAGGCCGCCTC
CAAAGTCCCGGCCGTGCGCCTACCGGGCCTCTTGAGCCTTCCCCCGGAGCTGCTGCTGGA
GATCTGTGCCTACCTGGATGCGCGAGTCGTGCTTCAAGTCCTGCCGTGCGTGTGCCAAGC
GTTGCACGACCTCGTGCGTGATCGTGTCACCTGGAGGCTACGCGCTCAGCGCCGCGTACG
CGCACCCTACCCAGTGGTGGAAGAGGAGAACTTTGACTGGCCAGCCGCCTGCATTGAGCT
GGAGCAGCACCTGGCCCGCTGGGCAGAGGATGGACAGCGAACCGAGTACTTCTGCCTGGC
TGATGGCCACTTTGCTTCCATTGATGCGGTGTTGCTGCTCCAGGGTGGGGCACTGTGTCT
GTCAGGCTCCCGGGATCGGAATGTCAACCTGTGGGACCTGCGCCATCTAGGGAAGGATCC
CAGCCGAGTTCTGGTGAAGGCCTTAGGCACCCAGGGCAATAGCACACACAAGGGCTGGGT
ATGGTCGCTAGCAGCGCAGGACCACCGTGTGTGCTCCGGCTCTTGGGACAGCACTGTGAA
GCTGTGGGACATGGCGGCTGATGGGCAGCAGTTTGGAGAGATCAAGGGCAAGGCGGCAGT
GCTGTGCCTTTCCTACCAACCTGATATCCTGGTGACTGGTACCTATGACAAGAAGGTGAC
CATCTATGATCCCAGAGCTGGTTTGGCCCTGGTGAAGAGCCGGAGGCTGCACTCAAGCGC
GGTGCTAGCAGTGCTGGCAGATGACAGGCATGTCATCTCAGGCAGCGAGGACCACAGCCT
TGTGGTATTTGATCGTCGAGCCAATAGCGTCCTACAGCGGCTGCAGCTGGACTCCTATCT
CCTGTGCATGTCTTACCAGGAGCCCCAGCTCTGGGCAGGTGACAACCAGGGTCTGCTGCA
CGTCTTCGCCAATCGAGATGGTTGCTTCCAGCTTGTTCGGTCCTTTGATGTGGGTCACCA
GTCTCAGATCACAGGGATCAAACACTCGCTGGGGACTTTGTACACAACATCTACTGACAA
GACCATTCGGGTTCACGTGCCCACAGATCCACCTAGGACCATCTGTACCCGAAGCCACCA
CAACGTGTTAAATGGGATCTGTGCTGAGGGCAACGTAGTGGTGGCTGCCTCTGGTGGCCT
GTCATTGGAGGTCTGGAGGCTGCTGGCCTAGGGCCTCATGGGCCCAGCTTTCTTGTAC

Protein sequence predicted based on nucleotide sequence

MDLSSGRSGDPRSCEEESDPEPDPDPDTQAEAYVARVLTPPKLGLTPRRSSLQSMFSASL
GVPERKAASKVPAVRLPGLLSLPPELLLEICAYLDARVVLQVLPCVCQALHDLVRDRVTW
RLRAQRRVRAPYPVVEEENFDWPAACIELEQHLARWAEDGQRTEYFCLADGHFASIDAVL
LLQGGALCLSGSRDRNVNLWDLRHLGKDPSRVLVKALGTQGNSTHKGWVWSLAAQDHRVC
SGSWDSTVKLWDMAADGQQFGEIKGKAAVLCLSYQPDILVTGTYDKKVTIYDPRAGLALV
KSRRLHSSAVLAVLADDRHVISGSEDHSLVVFDRRANSVLQRLQLDSYLLCMSYQEPQLW
AGDNQGLLHVFANRDGCFQLVRSFDVGHQSQITGIKHSLGTLYTTSTDKTIRVHVPTDPP
RTICTRSHHNVLNGICAEGNVVVAASGGLSLEVWRLLA*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

Start on reference sequence 10

End on reference sequence 1386

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223.1

Synonyms:

pDONR-223.1, pENTR223-star-Sfi

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php

Size (bp):

4311

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

CTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGAAGGGCCGTCAAGGCCCTGCAGACTGGCTGTGTATAAGGGAGCCTGACATTTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGCGGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTGGCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCGATATGCACCACCGGGTAAAGTTCACGGGAGACTTTATCTGACAGCAGACGTGCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATCACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGTAAACCTTAAACTGCATTTCACCAGTCCCTGTTCTCGTCAGCAAAAGAGCCGTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCGCTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTGCTTACCAGACCGGAGATATTGACATCATATATGCCTTGAGCAACTGATAGCTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTCATAGCACACCTCTTTTTGACATACTTCGGGTATACATATCAGTATATATTCTTATACCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCTGGCTTTTAGTAAGCCGGATCCACGCGATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCGATCAACGTCTCATTTTCGCCAAAAGTTGGCCCAGGGCTTCCCGGTATCAACAGGGACAGGCCTCATGGGCCCAGCTTTCTTGTACAAAGTTGGCATTATAAAAAATAATTGCTCATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTGCCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCG

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (Sfi sites flank the cassette present only in no insert form)

1265

1277

bacterial origin

ori

bacterial origin of replication

534

negative selection marker

ccdB

ccdB (no insert form only; lost in with insert form)

1312

1617

primer site

M13 F

M13 forward primer for insert sequencing

1113

1129

primer site

T7 Forward primer for insert sequencing

2902

2921

primer site

M13 reverse

M13 reverse primer for insert sequencing

2926

2942

recombination site

attL1

attL1 recombination site

1165

1264

recombination site

attL2

attL2 recombination site

2786

2885

selectable marker

CmR

chlR (no insert form only; lost in with insert form)

1959

2618

selectable marker

SpnR

spectinomycin resistance gene

3154

4164

Gene

Gene Symbol :

Fbxw9

Symbol Nomenclature :

Fbxw9

SYNONYM :

1110017H11Rik; Fbw9

Full Nomenclature :

F-box and WD-40 domain protein 9

GENEID :

68628

GI :

182887982

GenBank Accession :

BC160262

Mouse Genome Informatics :

1915878

Vega :

OTTMUSG00000031479

Target GenBank:

NM_026791.2

Protein

SMART domain :

FBOX : A Receptor for Ubiquitination Targets

SMART domain :

WD40 : WD40 repeats

UniProt :

Q6PAS7

Clone

Cloning Information :

481582

HIP Master Clone ID :

399310

IMAGE ID :

100063910

Original Clone ID :

FLH481582.01X

Organism

Species Specific ID:

68628

Genome

Chromosome :

Map Location :

8 C3|8

Ensembl :

ENSMUSG00000008167

Reagents

Labome :

Fbxw9-antibody