Gene:
Gene Symbol:
Pcdha10
Gene Name:
protocadherin alpha 10
Original Clone ID:
FLH481624.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00295377
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2841nts
Open reading frame: 35 to 2875
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGTCCTGTGTCGCCATGAAATACTG
CCATGAATCCTGGTGCCTGCTGCTCTCACTTTTGCTGTTCGCAATCTGGGAGCCTGGGAG
TGGCCAGCTCCGCTACTCCGTCCCAGAGGAGGCCAAACACGGCACCTTCGTGGGCCGCAT
CGCGCAGGACCTGGGGCTGGAGCTGACGGAGCTGGTGCCCCGCCTGTTCAGGGTGGCGTC
CAAGGACCGCGGGGACCTTCTGGAGGTAAATCTGCAGAATGGCATTTTGTTTGTGAATTC
TCGGATCGACCGGGAGGAGCTGTGCGGGCGGAGCGCGGAGTGCAGCATCCACCTGGAGGT
GATCGTGGACAGGCCGCTGCAGGTTTTCCACGTGGAGGTGGAGGTGAGGGATATTAATGA
CAACCCTCCCGTGTTTCCAACGACAGAAAAGAATCTCTTTGTTTCGGAATCGAGAGCTCT
AGATCCTCATTTTTCACTAGAGGGCGCTTCGGATGCAGACATCGGAACTAATGCTCTTCT
GACTTACAGACTGAGTCCCAGTGAATATTTCTCCCTGGAAGTACCAACCACAGATGAGTT
GGTAAAACCACTCCAACTAGTGTTAAAGAAACCTTTAGACAGAGAGCGAGCTTCAGAGCT
TCACTTAGTGGTTAAAGCCACGGATGGGGGCAAACCTGAGCTCACAGGCACCTTGGAGTT
ACATATTACAGTTCTAGATGCAAATGACAATGCGCCAGCATTCGACAGAGCAATCTATCG
GGTGAAGCTGGTAGAAAATGCAAGAAATGGTACCGTGGTCATTAGACTTAATGCCTCGGA
TTTGGACGAAGGTTCAAATGGTCAAATTCTTTATTCCTTTGCAGCGGATGTTTCCCCTAA
GACCGAAGCCACTTTTCATATAGATTCAGTCAGTGGAGAAATTAAAGTCAATGGAAAAAT
AGATTTTGAAGAAACTAATTTATGGAAGATTCAAGCAGAAGCAGTTGACAAAGGTAGTCC
TCCAATGTTTGGTCACTGCACAATCTTAATTGAAGTCCTGGACATCAATGATAATGCTCC
CAAGATAATAGTAACATCATTATCGCTCCCTGTTCAAGAGGACGCTCCAGTGGGGACAGT
TATTGCCTTAATCAGTGTGATGGATCCTGACTCTACAGTCAACGGGCAAGTGACCTGCTC
CCTGTCTCCTCATGTCCCCTTCAAGCTTGTGTCCACCTTCAAGAATTATTATTCCCTCGT
GCTGGACAGTGCTTTGGATCGAGAGACAACAGCTGACTATAAAGTGGTGGTGACAGCCCG
GGATGGGGGCTCTCCCGCGCTGTGGGCCACAGCCAGTGTGTCCGTGGAGGTGGCTGACGT
GAACGACAATGCTCCTGCATTCGCACAGTCGGAATACACGGTGTTCGTGAAGGAGAACAA
CCCGCCTGGAGTGCACGTCTTCACAGTATTGGCGATGGACGCGGATGCGCAGGAGAATGC
GCTGGTGTCCTACTCGCTGGTGGAGCGGAGGGTGGGCGAGCGCTTGCTGTCGAGCTATGT
GTCTGTGCACGCGGAAAGCGGCAAGGTGTTCGCGCTGCAGCCTCTGGACCATGAGGAGCT
GGAGCTGCTGCAGTTCCAGGTAACTGCGAAGGATGCTGGTGTGCCTGCCCTGGGCAGCAA
TGTGACTCTGCAGGTGTTTGTGCTGGATGAGAACGACAATGCACCCACCCTGCTGCCCCA
CGGAGCTGTCGGAGCTGGCGGGGCAGTGAGTGAGCTTGTGCCTAGGTCAGTGGGTTCAGG
GCATGTAGTGGCGAAGGTGCGGGCAGTAGATGCGGACTCTGGTTACAATGCTTGGTTGTC
ATATGAGCTCCAGTTCAGAGCTGGCAGTGTGCGCAGTCCCTTCCGTGTGGGACTATACAC
GGGTGAGATCAGTATCACTCGCGCTCTGGATGAAGCAGATGCGCCGCGCCAACGCCTGTT
GGTGTTGGTGAAGGACCATGGCGAGCCCGCGTTGACTGCCACAGCCACAGTGCTGGTTTC
TCTGGTTGAGAGCAACCAGGCACCAAAGGCCTCTTCACGGGTGTTGGGTCCAGCAGCCTC
TGAATCATCTGTAGTGGATGTCAACGTGTATTTGATCATCGCCATCTGTGCGGTGTCCAG
TCTGTTGGTGCTCACACTGGTGTTGTACACTGCCCTGCGATGCTCTGCATTGCCCACTGA
GGTCACCTGTGGACCCGGGAAACCCATGCTGGTATGTTCCAGCGCGGTGGGAAGCTGGTC
TTACTCTCAGCAAAGGAGGCAGAGGGTGTGTTCAGGAGAGGGCCCACCGAAGACAGATCT
CATGGCCTTCAGTCCCAGTGTTCCTCCTGGTTTGGGTTCTGGAGATAGTGGAGTCCAGCA
AGAGATCTTTGAGAATCCCCGGCAGCCCAACCCTGACTGGCGCTACTCTGCCTCGCTAAG
AGCAGGCATGCACAGCTCTGTGCACCTGGAGGAGGCTGGCATTCTACGGGCTGGTCCAGG
AGGGCCTGATCAGCAGTGGCCAACAGTATCCAGTGCAACACCAGAACCTGAGGCAGGAGA
GGTGTCCCCTCCGGTGGGCGCCGGTGTCAACAGCAACAGCTGGACCTTTAAATACGGACC
AGGCAACCCCAAACAGTCCGGTCCCGGTGAGTTGCCAGACAAATTCATTATCCCAGGATC
TCCTGCAATCATCTCCATCCGGCAGGAGCCTGCTAACAACCAAATTGACAAAAGCGATTT
TATAACCTTCGGCAAAAAGGAGGAGACCAAGAAAAAGAAGAAAAAGAAGAAGGGTAACAA
GACCCAGGAGAAAAAAGAGAAAGGGAACAGCACGACGGACAACAGTGACCAGTAGGGCCT
CATGGGCCCAGCTTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MSCVAMKYCHESWCLLLSLLLFAIWEPGSGQLRYSVPEEAKHGTFVGRIAQDLGLELTEL VPRLFRVASKDRGDLLEVNLQNGILFVNSRIDREELCGRSAECSIHLEVIVDRPLQVFHV EVEVRDINDNPPVFPTTEKNLFVSESRALDPHFSLEGASDADIGTNALLTYRLSPSEYFS LEVPTTDELVKPLQLVLKKPLDRERASELHLVVKATDGGKPELTGTLELHITVLDANDNA PAFDRAIYRVKLVENARNGTVVIRLNASDLDEGSNGQILYSFAADVSPKTEATFHIDSVS GEIKVNGKIDFEETNLWKIQAEAVDKGSPPMFGHCTILIEVLDINDNAPKIIVTSLSLPV QEDAPVGTVIALISVMDPDSTVNGQVTCSLSPHVPFKLVSTFKNYYSLVLDSALDRETTA DYKVVVTARDGGSPALWATASVSVEVADVNDNAPAFAQSEYTVFVKENNPPGVHVFTVLA MDADAQENALVSYSLVERRVGERLLSSYVSVHAESGKVFALQPLDHEELELLQFQVTAKD AGVPALGSNVTLQVFVLDENDNAPTLLPHGAVGAGGAVSELVPRSVGSGHVVAKVRAVDA DSGYNAWLSYELQFRAGSVRSPFRVGLYTGEISITRALDEADAPRQRLLVLVKDHGEPAL TATATVLVSLVESNQAPKASSRVLGPAASESSVVDVNVYLIIAICAVSSLLVLTLVLYTA LRCSALPTEVTCGPGKPMLVCSSAVGSWSYSQQRRQRVCSGEGPPKTDLMAFSPSVPPGL GSGDSGVQQEIFENPRQPNPDWRYSASLRAGMHSSVHLEEAGILRAGPGGPDQQWPTVSS ATPEPEAGEVSPPVGAGVNSNSWTFKYGPGNPKQSGPGELPDKFIIPGSPAIISIRQEPA NNQIDKSDFITFGKKEETKKKKKKKKGNKTQEKKEKGNSTTDNSDQ*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
2841
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
