Gene:
Gene Symbol:
Nek1
Gene Name:
NIMA (never in mitosis gene a)-related expressed kinase 1
Original Clone ID:
FLH481630.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00295383
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3612nts
Open reading frame: 35 to 3646
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGGAGAAGTATGTGAGACTGCAGAA
GATTGGAGAAGGTTCATTTGGAAAAGCTGTTCTTGTTAAATCGACAGAGGATGGCAGACA
TTATGTCATCAAGGAAATTAACATCTCAAGAATGTCTGATAAAGAAAGGCAAGAATCAAG
GAGAGAAGTTGCTGTATTGGCAAACATGAAGCATCCAAATATTGTCCAATATAAAGAATC
ATTTGAAGAAAATGGCTCTCTCTACATAGTAATGGATTACTGTGAAGGAGGTGATTTGTT
TAAACGAATAAATGCTCAGAAAGGCGCTCTGTTTCAAGAAGACCAGATTTTGGACTGGTT
TGTGCAGATATGTTTGGCTCTGAAGCATGTACATGATAGAAAAATTCTTCACCGAGACAT
AAAGTCACAGAACATATTTCTAACCAAAGATGGGACAGTGCAGCTTGGAGATTTTGGAAT
TGCTCGAGTTCTTAATAGTACTGTAGAGCTGGCTCGAACTTGCATAGGCACTCCATACTA
CTTGTCACCTGAAATCTGTGAAAACAAGCCTTATAACAATAAAAGTGACATTTGGGCTTT
GGGCTGTGTCCTTTATGAGTTGTGTACACTTAAACATGCATTTGAAGCTGGAAACATGAA
AAACCTGGTACTGAAGATAATCTCCGGATCCTTTCCTCCAGTGTCTCCACATTACTCCTA
TGATCTCCGCAGCTTGCTGTCTCAGTTATTTAAAAGAAATCCTAGGGATAGACCATCAGT
CAACTCCATATTGGAGAAAGGTTTTATAGCTAAACGAATCGAAAAGTTTCTCTCCCCTCA
GCTTATTGCAGAAGAATTTTGTCTAAAAACACTTTCAAAGTTTGGACCACAGCCTCTCCC
AGGTAAAAGACCAGCATCAGGACAAGGTGTCAGTTCTTTTGTCCCTGCTCAGAAAATCAC
AAAGCCTGCTGCTAAATACGGAGTGCCTTTAACATATAAGAAGTATGGAGATAAAAAGTT
ACTTGAGAAAAAACCACCCCCAAAACATAAACAGGCCCATCAAATTCCCGTGAAGAAAAT
GAATTCTGGAGAAGAAAGGAAGAAAATGTCTGAGGAAGCAGCAAAAAAAAGAAGGTTGGA
ATTTATTGAGAAAGAAAAGAAGCAAAAGGATCAGATTAGGTTCCTGAAGGCTGAGCAGAT
GAAGCGGCAAGAGAAGCAGCGGTTGGAGAGGATAAATAGGGCCAGGGAACAAGGATGGAG
GAATGTTTTAAGGGCTGGTGGAAGCGGTGAAGTAAAGGCTTCCTTTTTTGGCATTGGAGG
GGCTGTCTCTCCATCACCGTGTTCTCCTCGAGGCCAGTATGAACATTACCATGCCATTTT
TGACCAAATGCAGCGGCTAAGAGCAGAAGATAATGAAGCAAGATGGAAGGGGGGAATCTA
TGGTCGATGGCTCCCAGAAAGGCAAAAAGGACACTTAGCTGTAGAGAGAGCCAACCAAGT
GGAAGAATTCCTACAGCGTAAACGAGAAGCTATGCAGAATAAAGCCCGAGCCGAAGGACA
CGTGGTTTATTTGGCAAGACTGAGGCAAATAAGACTACAAAATTTTAATGAGCGCCAACA
GATTAAAGCCAAACTTCGTGGTGAGAATAAAGAAGCTGATGGTACCAAAGGACAAGAAGC
AACTGAAGAGACTGACATGAGGCTCAAAAAGATGGAGTCACTTAAGGCGCAAACAAATGC
ACGTGCTGCTGTACTAAAAGAACAGCTGGAGCGAAAAAGAAAGGAAGCTTATGAAAGAGA
AAAGAAAGTATGGGAAGAACATTTGGTGGCGAGGGTAAAAAGCTCAGATGTTCCTCTGCC
TTTGGAACTTCTTGAAACAGGTGGTTCTCCATCAAAGCAGCAGGTGAAGCCTGTCATTTC
TGTGACTTCAGCTTTGAAAGAAGTGGGCCTGGATGGAAGTTTAACTGATACCCAGGAAGA
AGAAATGGAAAAGAGTAACAGTGCTATTTCAAGTAAGCGAGAAATCCTGCGTAGGCTAAA
TGAAAATCTTAAAGCTCAAGAGGATGAAAAGGAAAAGCAGCATCACTCAGGTTCTTGTGA
GACCGTTGGTCACAAAGATGAGAGAGAGTATGAGACAGAAAATGCCATTTCCTCTGATCG
CAAGAAGTGGGAGATGGGAGGTCAGCTTGTGATTCCTCTCGATGCAGTGACACTGGATAC
ATCCTTCTCTGCAACCGAAAAACATACTGTGGGAGAGGTTATTAAATTAGATTCTAATGG
CTCTCCAAGAAAAGTCTGGGGGAAAAACCCTACAGATTCTGTGCTGAAGATACTTGGAGA
AGCTGAATTACAGCTACAGACAGAACTACTAGAAAACACATCTTTTAAAAGTGAGGTTTA
TGCTGAAGAGGAGAACTACAAACCCTTACTTACTGAAGAAGAGAATCTGCAGTGCATTTC
AAAAGAAATAAATCCATCAGCTACTGTTGATTCTACTGAAACGAAAAGTCCAAAGTTTAC
TGAGGTGTCTCCACAAATGTCAGAAGGAAATGTGGAAGAACCTGATGATTTGGAAACAGA
AGTTCTACAAGAGCCAAGTAGCACACACACAGATGGGAGTTTGCCACCTGTTCTTAATGA
TGTGTGGACTAGAGAGAAGGAAGCAGCTAAGGAAACTGAGTTGGAAGATAAGGTTGCTGT
GCAGCAGAGTGAAGTTTGTGAAGATAGAATTCCAGGGAACGTGGACCAATCCTGTAAGGA
TCAGAGAGATCCTGCAGTAGACGATTCTCCGCAGTCTGGCTGTGATGTAGAGAAGTCAGT
ACAGCCAGAATCGATTTTCCAGAAAGTGGTTCATTCTAAGGACTTGAACTTAGTTCAGGC
AGTTCATTGCTCACCAGAAGAACCAATTCCAATTCGATCTCACTCTGATTCTCCACCAAA
AACTAAGAGCAAGAATTCCTTACTGATTGGACTTTCAACTGGTCTGTTTGATGCAAACAA
TCCAAAGATGCTGAGGACCTGCTCACTTCCAGATCTTTCCAAGCTGTTCAGAACCCTAAT
GGACGTTCCCACTGTGGGGGACGTTCATCAAGACAGTCTTGAAATCGATGAGCTGGAAGA
TGAACCAATTAAAGAAGGGCCTTCTGATTCCGAAGACACTGTATTTGAAGAAACTGACAC
AGATTTACAAGAGCTTCAGGCCTCAATGGAGCAGCTGCTTAGGGAGCAACCAGGTGACGA
ATACAGTGAGGAGGAAGAGTCTGTTTTAAAAAGCAGCGATGTGGAGCAGACAGCAAGAGG
GACAGATGCCCCAGACGAGGAGGACAACCCCAGCAGCGAAAGCGCCCTGAACGAGGAATG
GCACTCAGATAATAGTGACGCTGAGACCACTAGTGAATGTGAATATGACAGTGTCTTTAA
CCATTTAGAGGAACTAAGACTTCACTTGGAGCAAGAAATGGGCTTTGAAAAGTTCTTTGA
GGTTTATGAGAAAGTAAAGGCTATTCATGAGGATGAAGATGAAAATATTGAAATTTGTTC
AACAATAGTTGAGAATATTTTGGGCAATGAGCACCAGCATCTCTATGCCAAGATTCTGCA
TTTAGTCATGGCAGATGGAGCCTATCAGGAAGATAATGATGAATAGGGCCTCATGGGCCC
AGCTTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MEKYVRLQKIGEGSFGKAVLVKSTEDGRHYVIKEINISRMSDKERQESRREVAVLANMKH PNIVQYKESFEENGSLYIVMDYCEGGDLFKRINAQKGALFQEDQILDWFVQICLALKHVH DRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPY NNKSDIWALGCVLYELCTLKHAFEAGNMKNLVLKIISGSFPPVSPHYSYDLRSLLSQLFK RNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTLSKFGPQPLPGKRPASGQGVS SFVPAQKITKPAAKYGVPLTYKKYGDKKLLEKKPPPKHKQAHQIPVKKMNSGEERKKMSE EAAKKRRLEFIEKEKKQKDQIRFLKAEQMKRQEKQRLERINRAREQGWRNVLRAGGSGEV KASFFGIGGAVSPSPCSPRGQYEHYHAIFDQMQRLRAEDNEARWKGGIYGRWLPERQKGH LAVERANQVEEFLQRKREAMQNKARAEGHVVYLARLRQIRLQNFNERQQIKAKLRGENKE ADGTKGQEATEETDMRLKKMESLKAQTNARAAVLKEQLERKRKEAYEREKKVWEEHLVAR VKSSDVPLPLELLETGGSPSKQQVKPVISVTSALKEVGLDGSLTDTQEEEMEKSNSAISS KREILRRLNENLKAQEDEKEKQHHSGSCETVGHKDEREYETENAISSDRKKWEMGGQLVI PLDAVTLDTSFSATEKHTVGEVIKLDSNGSPRKVWGKNPTDSVLKILGEAELQLQTELLE NTSFKSEVYAEEENYKPLLTEEENLQCISKEINPSATVDSTETKSPKFTEVSPQMSEGNV EEPDDLETEVLQEPSSTHTDGSLPPVLNDVWTREKEAAKETELEDKVAVQQSEVCEDRIP GNVDQSCKDQRDPAVDDSPQSGCDVEKSVQPESIFQKVVHSKDLNLVQAVHCSPEEPIPI RSHSDSPPKTKSKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVHQD SLEIDELEDEPIKEGPSDSEDTVFEETDTDLQELQASMEQLLREQPGDEYSEEEESVLKS SDVEQTARGTDAPDEEDNPSSESALNEEWHSDNSDAETTSECEYDSVFNHLEELRLHLEQ EMGFEKFFEVYEKVKAIHEDEDENIEICSTIVENILGNEHQHLYAKILHLVMADGAYQED NDE*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
4187
Start on reference sequence
576
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
