DNASU Plasmid | Asb18 (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: Asb18

Gene Name: ankyrin repeat and SOCS box-containing 18

Original Clone ID:

FLH481636.01X

Keyword:

None

Species:

Mus musculus

Type:

cDNA

Vector Name:

pENTR223.1

Format:

CLOSED

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

MmCD00295389

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1404nts

Open reading frame: 35 to 1438

GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGTCCAGCTGGGACTCGCCCCCAGA
TTACCCCCTCAGCTCAGACATAGTGAGGAGATTAAAGTCTGCTCTGGCCACTGGGGACGA
GGACACTGTACAGAAGCTGATCTGCACTGAGGTGGAGCCTGTGGACGCCGTAATAGAACT
TGCTAATGATGACTGGATGAAGGAGCCCTCAGCCGAGCTGCCCACGGGCGTACTGCTAGG
AGACCTGGACCACATCCGGCTCCTCATGGACCAGTGCTTCTGGGATGCCAACGCGGTGTT
TGAGATCAGTAACGGAGAGATGGAATGGCAGGTGACATCCCTGGCCACCTTTGGGCTCTC
AGGCCTTTGGACCCTGGAGTACCAGCGGGAGCTCACCACACCGCTGTGCATCGCTGCGGC
CCACGGCCACGAGTCCTGCGTGCGACACCTGCTCAGTCGCAAAGCTGATCCCAATGCCAG
TCCCGGAGGTCGTGGTGCCCTACATGAGGCCTGTCTCGGGAACCATACCGCCTGCGCCCG
ACTGCTGCTGCAGCACGGAGCAGATCCAGATCTGCTCAACTCCGAGGGCCTGGCACCCCT
GCACCTATGCCGCACACCCGCCTCGCTTGGGTGCGCTCGGGAGCTGCTGGAGCACGGCGC
GACAGTCCAGCTGGAGGGTGGCCCTGGCCGAGACACACCGCTGCACGTGGCGGCCCAGCG
CGGCCTGGACGAGCACGCGCAACTCTACCTGGGCTTCGGCGCGCGAGTGGACGCGAGGAA
CGGCCGGGGCGAAACGGCCCTTAGCGCAGCATGCGGAGCGGCGGCGCGGAGCCCCGACGA
GCACGCGCGCTGCCTGCGCCTGTGCGCGCTCCTTCTGCGCGGCGGCGCGGCGGCAGACGC
GCGTGACGAGGACGAGCGCAGCCCGCTGCACAAGGCCTGCGGCCACGCGCGCCCCGGCCT
GTGCACGCTCCTGCTGCGGCACGGCGCCGACGCGGGCGCGCTCGACTATGGTGGCGCGTC
CCCGCTGGCGCGCGCGCTGCAGACGGCCACGTGCGCGCCCCCGACAGCGCCGCAGCGCAC
GGTCCAGGTGCTACTAAATCACGGATCCCGCTCCGTGTGGCCTGATGCCTTCCCCAAGGT
TCTGAGGACATGTGCACAGGTGCCTGAAGTCATTGAAGTTCTGTTCAACTCCTACACTCA
GCTCCCAGTGTCTGAGTCCTGGCGGGAAGCCATCCCTGAAGAAGTATACCAGATGCACAA
GCCTTTCTACAGGTCTCTCTTCGCCTTGGCACACACCCCGCGCTGCCTGCAGCACCTTTG
CCGCTGTACTATCCGAAAACTCTTCGGCAAGGAGAGCGTTCACCTTGTGCCCCAGCTACC
ATTACCTGAGACTCTGCAAAATTACCTGCTTTTGGAGCCAGAGGGTGTTTTGTGTTAGGG
CCTCATGGGCCCAGCTTTCTTGTAC

Protein sequence predicted based on nucleotide sequence

MSSWDSPPDYPLSSDIVRRLKSALATGDEDTVQKLICTEVEPVDAVIELANDDWMKEPSA
ELPTGVLLGDLDHIRLLMDQCFWDANAVFEISNGEMEWQVTSLATFGLSGLWTLEYQREL
TTPLCIAAAHGHESCVRHLLSRKADPNASPGGRGALHEACLGNHTACARLLLQHGADPDL
LNSEGLAPLHLCRTPASLGCARELLEHGATVQLEGGPGRDTPLHVAAQRGLDEHAQLYLG
FGARVDARNGRGETALSAACGAAARSPDEHARCLRLCALLLRGGAAADARDEDERSPLHK
ACGHARPGLCTLLLRHGADAGALDYGGASPLARALQTATCAPPTAPQRTVQVLLNHGSRS
VWPDAFPKVLRTCAQVPEVIEVLFNSYTQLPVSESWREAIPEEVYQMHKPFYRSLFALAH
TPRCLQHLCRCTIRKLFGKESVHLVPQLPLPETLQNYLLLEPEGVLC*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

Start on reference sequence 1

End on reference sequence 1404

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223.1

Synonyms:

pDONR-223.1, pENTR223-star-Sfi

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php

Size (bp):

4311

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

CTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGAAGGGCCGTCAAGGCCCTGCAGACTGGCTGTGTATAAGGGAGCCTGACATTTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGCGGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTGGCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCGATATGCACCACCGGGTAAAGTTCACGGGAGACTTTATCTGACAGCAGACGTGCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATCACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGTAAACCTTAAACTGCATTTCACCAGTCCCTGTTCTCGTCAGCAAAAGAGCCGTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCGCTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTGCTTACCAGACCGGAGATATTGACATCATATATGCCTTGAGCAACTGATAGCTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTCATAGCACACCTCTTTTTGACATACTTCGGGTATACATATCAGTATATATTCTTATACCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCTGGCTTTTAGTAAGCCGGATCCACGCGATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCGATCAACGTCTCATTTTCGCCAAAAGTTGGCCCAGGGCTTCCCGGTATCAACAGGGACAGGCCTCATGGGCCCAGCTTTCTTGTACAAAGTTGGCATTATAAAAAATAATTGCTCATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTGCCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCG

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (Sfi sites flank the cassette present only in no insert form)

1265

1277

bacterial origin

ori

bacterial origin of replication

534

negative selection marker

ccdB

ccdB (no insert form only; lost in with insert form)

1312

1617

primer site

M13 F

M13 forward primer for insert sequencing

1113

1129

primer site

T7 Forward primer for insert sequencing

2902

2921

primer site

M13 reverse

M13 reverse primer for insert sequencing

2926

2942

recombination site

attL1

attL1 recombination site

1165

1264

recombination site

attL2

attL2 recombination site

2786

2885

selectable marker

CmR

chlR (no insert form only; lost in with insert form)

1959

2618

selectable marker

SpnR

spectinomycin resistance gene

3154

4164

Gene

Gene Symbol :

Asb18

Symbol Nomenclature :

Asb18

SYNONYM :

Asb-18

Designation :

SOCS box protein ASB-18|ankyrin repeat and SOCS box protein 18

Full Nomenclature :

ankyrin repeat and SOCS box-containing 18

GENEID :

208372

GI :

189442782

GenBank Accession :

BC167192

Mouse Genome Informatics :

2655109

Vega :

OTTMUSG00000026747

Target GenBank:

NM_139152.1

Protein

SMART domain :

ANK : ankyrin repeats

SMART domain :

SOCS_box :

UniProt :

Q8VHA6

Clone

Cloning Information :

481636

HIP Master Clone ID :

399364

IMAGE ID :

100068092

Original Clone ID :

FLH481636.01X

Organism

Species Specific ID:

208372

Genome

Chromosome :

Map Location :

1 D|1

Ensembl :

ENSMUSG00000067081

Reagents

Labome :

Asb18-siRNA