Gene:
Gene Symbol:
Gltscr1
Gene Name:
glioma tumor suppressor candidate region gene 1
Original Clone ID:
FLH481657.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00295410
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4737nts
Open reading frame: 35 to 4771
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGGATGATGAGGATGGGAGATGCTT
ACTAGACGTGATTTGTGATCCTCAGGCCCTCAATGATTTCTTGCATGGATCCGAGAAGCT
GGACAGCGATGACCTCCTGGATGCCCCTGTGGAGGCCCAAAGTGCCTTCTATGAAGGTCC
TGGGCTCCATGTGCAGGAAGCTGCCGCCAACCACCTAAACCCTGAGCCCAGCCAGCCTGC
CCCCAGCGTGGACCTGGACTTCCTAGAAGATGATATCTTGGGCTCCCCTGCAGCAGGAGG
AGGTGGAGGGGGCGGCGGGGCCCCAGACCAGCCCTGTGACATCCTTCAGCAGAGTCTTCA
GGAGGCCAACATCACAGAACAGACCCTGGAGGCTGAGGCTGAACTGGACCTGGGCCCCTT
CCAGCTGCCCACCCTACAGCCCGCTGACAATGGGGCAGGTGCTACTGGAGCCGCAGGAGC
CACGGCAGTGACTGCAGGACCCCAGGCTCTCTTCCCAGGCAGCGCGGATCTGCTGGGGCT
GCAAGCCCCGCCCACTGTACTGACCCACCAGGCCCTGGTGCCACCCCAGGATGTGGTCAA
CAAGGCCTTGAGCGTCCAGCCCTTCCTGCAGCCTGTGGGCCTGGGCAATGTGACCCTTCA
GCCCATTTCAGGCCTCCAGGGCCTTCCCAATGGCAGTCCTGGGAATGCTGCAGCAGCCAC
CTTGGGTCTGACACCTATTCAAGTGGTGGGCCAGCCCGTCATGGCTCTCAACCCACCCAC
CTCCCAGCTCTTGGCAAAGCAGGTACCTGTCAGTGGCTACCTGGCCTCAGCAGCTGGTCC
TTCAGAGCCAGTGACACTGGCATCTGCCGGCGTGTCCCCCCAGGGAGCCGGCCTGGTCAT
CCAGAAAAATCTTCCAGCCGCAGTGACCACCACACTCAACGGGAACTCGGTGTTTGCCGG
GACAGGGGCTGCCACTGCAGCAGCCAGTGGGGCACCCTCGGGACAGCCGCTGGCGGTGGC
CCCGGGCCTTGGCACATCACCACTGGTACAAGCACCCAGTGTGATTTTACACAGAACCCC
TACGCCTATCCAGCCCAAGCCTACAGGGGTCCTGCCCTCCAAACTCTACCAGCTGACACC
CAAGCCCTTTCCCCCTACCGGAGCCACCCTTACCATCCAGGGTGAACCAGGCACCTTGCC
CCAGCAGCCTAAGGCCCCCCAGAACCTGACTTTTATGGCCACGGGCAAAGCTGGCCAGAA
TGTGGTGCTGTCTGGCTTCCCGGCACCGGCTTTGCAGGCGAATGTGTTCAAGCAGCCACC
AGTCACCACCACGGGGACAGCCCCGCCACAGCCACCAGGGGCCCTCAGCAAACCCATGAG
CGTCCACCTCCTCAATCAAGGCAGCAGCATCGTGATCCCAGCCCAGCACATGCTGCCTGG
CCAGAACCAGTTCTTGCTGCCAGGCACCCCAGCCGTACAACTCCCTCAGTCACTCTCTGC
ACTGCCTGCCAACGTGGGAGGCCAGATCCTCACAGCTGCAGCACCACACGCAGGTGGACA
GCTCATTGCCAACCCTATCCTCACCAACCAGAACCTGGCAGGCCCACTGAGTCTGGGCCC
AGTGCTGGCACCCCACTCTGGGGCACACAGCGCTGCACACATCCTCTCTGCAGCTCCCAT
CCAGGTGGGCCAGCCTGCCCTCTTCCAGATGCCTGTGTCACTGGCCACTGGCAGCCTGCC
TACTCAGAGCCAGCCGGCTCCCACTGGCCCCACAGCCACCACCGTCCTCCAGGGCGTCAC
CCTGCCTCCCAGTGCTGTGGCCATGCTTAACACGCCTGATGGGCTAGTGCAACCCTCCAC
TCCAGCTGCCACCACTGGGGAGGCCACACCAGTTCTGGCCGTTCAGCCTGCAACCCAGGT
GCCCCCTGCTGTCACCACACCACTGCCTATGGGTCTCCAACAGCCACAGGCACAGCAGCC
TCCACAGGTCCCTACTCCACAGGCGGCCACCCAGCCTCAGGCCACCCCTCCTCAGGCCAG
CCCAAGCCTGGCTTCCAGCCCAGAGAAGATAGTCCTGGGGCAGGCGCCCCCTGCGGCCAC
AACGGCCATCCTCACTCAGGATTCCCTACAGATGTTCCTGCCCCAGGAGAGGAGCCAGCA
GCCCCTCTCTACAGAGGGTCCCCACCTCTCGGTGCCTGCCTCCGTCATAGTCAGCGCCCC
GCCTCCTGCCCAAGACCCAGCCCTGGCCACGCCCGTCACCAAAGGAGCTGGCCTCGGCGC
TCAGACCCCGGACAGCCGGGCTTCCCCAGCTCCGGCTCCCCAGATCCCTGCAGCTGCTCC
ACTGAAAGCCCCTGGCCCCGCCTCCTCCCCCTCACTACCTCACCAGGCCCCCCTGGGAGA
CAGTCCCCACATGCCCTCCCCACACCCTGCCAGGCCCCCTTCCCGCCCACCCTCAAGACC
CCACTCACGCCCTCCATCCCAGCCCCAGAGCCTGACCTGCCCACCCTCTGAGCCCACCCT
GCACCCTTGCCCTCCACCCCAGGGTCCCCCAACTCTACCTGGCATCTTTGTCATCCAGAA
TCAATTGGGCGCCCCACCACCAGCCAGCACCCCAGCCTCCACAGCCCCGGGCCCACCCCA
GCCTCCTCTGCGACCCCCATCCCAGCCTCCAGAGGGCCCACTGCCCCCAGCCTCCCACCT
CCCTCCTGCCTCCACCCCCTCGGCCGTGGCCTCCTCCTCTGAGCCTTCTGCCAGGTTGCC
GGTCCCCACACCCCCTGACTTCCAACTCCAGTTCCCACCGAGCCAGGGACCCCATAAGTC
CCCTACTCCGCCACCAGCCCTCCACATGGTCCCTGAGCCCACGGCACCCCCTCCTCCACC
ACCTCGGACCTTCCAGATGGTAACCGCCCCCTTCCCAGCGTTGCCCCAGCCAAAAGCACT
TCTGGAACGATTCCACCAGGTGCCATCTGGGATTATTCTCCAGAATAAGGCTGGGGGTAC
TCCCACCACCCCACAGACATCCACCACCCTGGGGACCCTCACCGGTCCTACTGCCTCTGT
GCTAGTCAGTGGACAGGCACCACCTGGGACTCCTGCCGCCTCTAGCCATGTCCCAGCCTC
CACACCTATGGCCACCACAGGCCTCCCTCCTCTACTTCCTGCCGAAAACAAAGCTTTTGC
CAGCAACCTTCCAACCCTGAGTGTGGCCAAAGCTACCGTGTCTGGGCCAGGGAAGCCCCC
AGCAATTCAGTATGACAGCAAGTTGTGTAGCTTGAAGAAACAGCCCCTACTGCAACCCAG
CAAAGAAGCCTGCTTCCTGGAGCATCTGCACAAACACCAGGGCTCTGTCCTGCACCCCGA
TTACAAGACAGCCTTCCCCTCCTTTGAGGACGCCCTCCATCGCCTCCTGCCCTACCATGT
CTACCAAGGCGCCCTCCCCTCCCCCAACGACTACCATAAAGTGGATGAAGAATTTGAGAC
TGTCTCTACGCAGCTGCTCAAACGCACCCAGGCCATGCTCAATAAATATCGGCTTTTGCT
TCTGGAAGAGTCCAGGAGAGTCAGTCCTTCTGCGGAGATGGTTATGATCGACCGAATGTT
CATTCAGGAGGAGAAGACCACCCTTGCCTTGGATAAGCAGCTTGCCAAGGAGAAGCCTGA
TGAGTACGTGTCTTCCTCCCGCTCCCTTGGCTTCCCTGTCCCAGTGTCTTCCGAGGGCCA
CCGGCTCCCCAGCCATGGCCAGTCGTCTTCATCCTCCACATCTGGAACGTCTGCCCAGCC
CCCTCCTCATCTGCCCACCAAGCTAGTGATCCGGCACGGTGGGGCCGGCGGCTCTCCCTC
AGTGACCTGGGCCCGGGCATCCTCCTCCTTGTCATCCACTTCCTCATCCTCCTCCTCATC
CTCTGCTGCCTCATCCCTGGACGCAGATGAGGACGGCCCCATGCCCACCCGTAACCGGCC
ACCCATCAAGACCTATGAGGCCCGGAGCCGCATTGGTCTCAAACTCAAGATCAAACAAGA
GGCGGGGCTCAGCAAGGTGGTGCACAACACTGCACTGGATCCTGTGCATCAGCCCTTGCC
GGCTCCAACCCCAGCGAAAGGGGCGGAGCCTCCGCCACACCCAGCTCCGCCCCCACTCCC
TCCTGCTACCCAGGCGCAGATGAATGGCACTCTGGACCATCCCCCACCCGCAGTACGCAA
ACCCACGGTGCCTGCGTCCTGCCCACGTCTACCACTACGCAAGACCTACCGAGAAAACAT
GGGCAATCCTGGTGCCGCCGAGGGTGCACAGGGACGGCCGCGGGGTGCGGGCAGCCCCAC
CCCACTGCCCACCAAGGTAGACGAAGCCACCAGTGGGCTGATCCGGGAGCTGGCAGCGGT
GGAGGATGAACTATATCAGCGGGTTCTGAAGGGCGGCCCACCACCCCCGGAGACTCCAGC
CTCCGCTACCAGCCAGGGCCCCACTGAACCCAGTTGGGAAGCACCCGTGCTACCCCCAGC
CAAACGACGCAAGTCTGAGTCCCCGGACGTGGACCAGGCCAGCTTCTCTAGTGACAGCCC
GCAGGATGATACACTTACTGAGCATTTGCAGAGTGCCATCGACAGCATCCTTAACCTGCA
GCAGGCCCCCGGCCGGACACCCGCAGGCCCATACCCCCATACGGGGCCCACGCCTGGCAC
CCCCACATCCCCAGCGCCCCTGCACAGGCCTGACGCCTTCCCACCCTCTAGTCACAATGG
TGGCCTCGGTGCCAGGACGTTGAACAGATAGGGCCTCATGGGCCCAGCTTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MDDEDGRCLLDVICDPQALNDFLHGSEKLDSDDLLDAPVEAQSAFYEGPGLHVQEAAANH LNPEPSQPAPSVDLDFLEDDILGSPAAGGGGGGGGAPDQPCDILQQSLQEANITEQTLEA EAELDLGPFQLPTLQPADNGAGATGAAGATAVTAGPQALFPGSADLLGLQAPPTVLTHQA LVPPQDVVNKALSVQPFLQPVGLGNVTLQPISGLQGLPNGSPGNAAAATLGLTPIQVVGQ PVMALNPPTSQLLAKQVPVSGYLASAAGPSEPVTLASAGVSPQGAGLVIQKNLPAAVTTT LNGNSVFAGTGAATAAASGAPSGQPLAVAPGLGTSPLVQAPSVILHRTPTPIQPKPTGVL PSKLYQLTPKPFPPTGATLTIQGEPGTLPQQPKAPQNLTFMATGKAGQNVVLSGFPAPAL QANVFKQPPVTTTGTAPPQPPGALSKPMSVHLLNQGSSIVIPAQHMLPGQNQFLLPGTPA VQLPQSLSALPANVGGQILTAAAPHAGGQLIANPILTNQNLAGPLSLGPVLAPHSGAHSA AHILSAAPIQVGQPALFQMPVSLATGSLPTQSQPAPTGPTATTVLQGVTLPPSAVAMLNT PDGLVQPSTPAATTGEATPVLAVQPATQVPPAVTTPLPMGLQQPQAQQPPQVPTPQAATQ PQATPPQASPSLASSPEKIVLGQAPPAATTAILTQDSLQMFLPQERSQQPLSTEGPHLSV PASVIVSAPPPAQDPALATPVTKGAGLGAQTPDSRASPAPAPQIPAAAPLKAPGPASSPS LPHQAPLGDSPHMPSPHPARPPSRPPSRPHSRPPSQPQSLTCPPSEPTLHPCPPPQGPPT LPGIFVIQNQLGAPPPASTPASTAPGPPQPPLRPPSQPPEGPLPPASHLPPASTPSAVAS SSEPSARLPVPTPPDFQLQFPPSQGPHKSPTPPPALHMVPEPTAPPPPPPRTFQMVTAPF PALPQPKALLERFHQVPSGIILQNKAGGTPTTPQTSTTLGTLTGPTASVLVSGQAPPGTP AASSHVPASTPMATTGLPPLLPAENKAFASNLPTLSVAKATVSGPGKPPAIQYDSKLCSL KKQPLLQPSKEACFLEHLHKHQGSVLHPDYKTAFPSFEDALHRLLPYHVYQGALPSPNDY HKVDEEFETVSTQLLKRTQAMLNKYRLLLLEESRRVSPSAEMVMIDRMFIQEEKTTLALD KQLAKEKPDEYVSSSRSLGFPVPVSSEGHRLPSHGQSSSSSTSGTSAQPPPHLPTKLVIR HGGAGGSPSVTWARASSSLSSTSSSSSSSSAASSLDADEDGPMPTRNRPPIKTYEARSRI GLKLKIKQEAGLSKVVHNTALDPVHQPLPAPTPAKGAEPPPHPAPPPLPPATQAQMNGTL DHPPPAVRKPTVPASCPRLPLRKTYRENMGNPGAAEGAQGRPRGAGSPTPLPTKVDEATS GLIRELAAVEDELYQRVLKGGPPPPETPASATSQGPTEPSWEAPVLPPAKRRKSESPDVD QASFSSDSPQDDTLTEHLQSAIDSILNLQQAPGRTPAGPYPHTGPTPGTPTSPAPLHRPD AFPPSSHNGGLGARTLNR*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
108
End on reference sequence
4844
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
