DNASU Plasmid | Clint1 (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: Clint1

Gene Name: clathrin interactor 1

Original Clone ID:

FLH481671.01X

Keyword:

None

Species:

Mus musculus

Type:

cDNA

Vector Name:

pENTR223.1

Format:

CLOSED

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

MmCD00295424

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1872nts

Open reading frame: 35 to 1906

GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGCTGAACATGTGGAAGGTGCGGGA
GCTGGTGGACAAAGCCACCAATGTGGTTATGAATTACTCAGAGATTGAGTCTAAGGTTCG
AGAAGCAACGAATGATGATCCCTGGGGACCTTCTGGGCAGCTCATGGGAGAGATTGCCAA
GGCTACCTTTATGTATGAGCAGTTCCCAGAACTCATGAACATGCTTTGGTCCCGGATGCT
AAAAGACAACAAAAAGAACTGGAGAAGAGTTTATAAGTCGTTGCTGCTCCTAGCTTACCT
CATAAGAAATGGATCGGAGCGAGTTGTTACAAGTGCCAGAGAACACATTTATGATTTGCG
ATCCCTGGAAAATTACCACTTTGTAGATGAGCATGGCAAGGATCAAGGTATAAACATTCG
ACAGAAAGTAAAGGAACTGGTTGAGTTTGCACAGGATGATGACAGGCTTCGAGAAGAGCG
AAAGAAAGCAAAGAAGAACAAAGACAAGTATGTCGGGGTCTCTTCAGACAGCGTTGGTGG
ATTCCGATACAATGAGCGGTACGATCCTGAGCCCAAGTCCAAATGGGACGAAGAGTGGGA
TAAGAACAAGAGCGCGTTTCCGTTCAGTGACAAGCTGGGCGAGCTGAGCGACAAGATCGG
GAGCACGATCGACGACACCATCAGCAAGTTCCGGAGGAAAGACCGGGAGGACTCTCCGGA
AAGGTGCAGCGACAGTGATGAGGAAAAGAAAGCAAGAAGAGGTAGATCTCCCAAAGGTGA
ATTCAAAGATGAAGAGGAAACGGTGACGACGAAGCATATCCACATCACACAGGCCACAGA
GACCACCACAACCCGGCACAAGCGCACAGCAAACCCTTCCAAAACCATTGATCTTGGAGC
AGCAGCACATTACACGGGGGACAAAGCCAGCCCAGATCAGAATGCTTCAACCCACACGCC
TCAGTCGTCAGCCAAGCCATCGGTGCCGAGCAGCAAGTCCTCGGGGGACCTCGTTGACCT
GTTTGATGGCAGTAGCCAGTCAGCAGGTGGATCAGCTGATTTATTTGGGGGATTTGCTGA
CTTTGGCTCAGCTGCTGCATCAGGCAATTTCCCTTCCCAAGCAACAAGTGGGAATGGTGA
CTTTGGTGACTGGAGTGCCTTCAACCAAGCCCCATCAGGCCCTGTGGCTTCTGGTGGTGA
GCTCTTCGGCAGTGCCCCACAGTCGGCTGTGGAGCTTATCAGTGCTTCACAGCCAGCTTT
AGGCCCACCGCCTGCTGCCTCAAACTCGGCAGACCTGTTTGATCTTATGGGCTCATCCCA
GGCAACCATGACGTCTTCCCAGAGCATGAATTTCTCTCTCATGAGCACTAACACCGTGGG
ACTTGGTTTGCCCATGTCCAGATCACAGAATACAGATATGGTCCAGAAGTCGGCCAGCAA
AACCCTGCCCTCTACTTGGTCTGACCCCAGTGTCAACATCAGCCTAGACAACTTACTACC
TGGTATGCAGCCTTCCAAGCCCCAGCAGCCATCGCTTAATACAATGATTCAACAACAGAA
TATGCAACAGCCTCTGAATGTGATGACACAAAGCTTTGGAGCTGTGAACCTCAGTTCTCC
ATCAAACATGCTTCCTGTCCGGCCTCAAACTAACCCTCTCCTGGGGGGACCCATGCCTAT
GAACATGCCCGGTGTAATGACGGGCACCATGGGAATGGCCCCTCTGGGGAACAGCGCAGG
GATGAGCCAGGGCATGGTGGGCATGAACATGAACATGGGGATGTCGGCCTCGGGGATGGG
CTTGTCGGGCACCATGGGAATGGGCATGCCCAGCATGGCCATGCCTTCTGGAACTGTGCA
ACCCAAGCAAGATGCCTTTGCAAACTTTGCCAACTTTAGCAAATAGGGCCTCATGGGCCC
AGCTTTCTTGTAC

Protein sequence predicted based on nucleotide sequence

MLNMWKVRELVDKATNVVMNYSEIESKVREATNDDPWGPSGQLMGEIAKATFMYEQFPEL
MNMLWSRMLKDNKKNWRRVYKSLLLLAYLIRNGSERVVTSAREHIYDLRSLENYHFVDEH
GKDQGINIRQKVKELVEFAQDDDRLREERKKAKKNKDKYVGVSSDSVGGFRYNERYDPEP
KSKWDEEWDKNKSAFPFSDKLGELSDKIGSTIDDTISKFRRKDREDSPERCSDSDEEKKA
RRGRSPKGEFKDEEETVTTKHIHITQATETTTTRHKRTANPSKTIDLGAAAHYTGDKASP
DQNASTHTPQSSAKPSVPSSKSSGDLVDLFDGSSQSAGGSADLFGGFADFGSAAASGNFP
SQATSGNGDFGDWSAFNQAPSGPVASGGELFGSAPQSAVELISASQPALGPPPAASNSAD
LFDLMGSSQATMTSSQSMNFSLMSTNTVGLGLPMSRSQNTDMVQKSASKTLPSTWSDPSV
NISLDNLLPGMQPSKPQQPSLNTMIQQQNMQQPLNVMTQSFGAVNLSSPSNMLPVRPQTN
PLLGGPMPMNMPGVMTGTMGMAPLGNSAGMSQGMVGMNMNMGMSASGMGLSGTMGMGMPS
MAMPSGTVQPKQDAFANFANFSK*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

Start on reference sequence 189

End on reference sequence 2060

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223.1

Synonyms:

pDONR-223.1, pENTR223-star-Sfi

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php

Size (bp):

4311

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

CTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGAAGGGCCGTCAAGGCCCTGCAGACTGGCTGTGTATAAGGGAGCCTGACATTTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGCGGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTGGCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCGATATGCACCACCGGGTAAAGTTCACGGGAGACTTTATCTGACAGCAGACGTGCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATCACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGTAAACCTTAAACTGCATTTCACCAGTCCCTGTTCTCGTCAGCAAAAGAGCCGTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCGCTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTGCTTACCAGACCGGAGATATTGACATCATATATGCCTTGAGCAACTGATAGCTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTCATAGCACACCTCTTTTTGACATACTTCGGGTATACATATCAGTATATATTCTTATACCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCTGGCTTTTAGTAAGCCGGATCCACGCGATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCGATCAACGTCTCATTTTCGCCAAAAGTTGGCCCAGGGCTTCCCGGTATCAACAGGGACAGGCCTCATGGGCCCAGCTTTCTTGTACAAAGTTGGCATTATAAAAAATAATTGCTCATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTGCCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCG

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (Sfi sites flank the cassette present only in no insert form)

1265

1277

bacterial origin

ori

bacterial origin of replication

534

negative selection marker

ccdB

ccdB (no insert form only; lost in with insert form)

1312

1617

primer site

M13 F

M13 forward primer for insert sequencing

1113

1129

primer site

T7 Forward primer for insert sequencing

2902

2921

primer site

M13 reverse

M13 reverse primer for insert sequencing

2926

2942

recombination site

attL1

attL1 recombination site

1165

1264

recombination site

attL2

attL2 recombination site

2786

2885

selectable marker

CmR

chlR (no insert form only; lost in with insert form)

1959

2618

selectable marker

SpnR

spectinomycin resistance gene

3154

4164

Gene

Gene Symbol :

Clint1

Symbol Nomenclature :

Clint1

SYNONYM :

AI642036; AW537616; C530049I24Rik; Enth; Epn4; F630206G17Rik; mKIAA0171

Designation :

Full Nomenclature :

clathrin interactor 1

GENEID :

216705

Locus Tag :

RP23-298M7.4

GI :

189442100

GenBank Accession :

BC167227

Mouse Genome Informatics :

2144243

Vega :

OTTMUSG00000005479

Target GenBank:

NM_001045520.3

Protein

SMART domain :

ENTH : Epsin N-terminal homology (ENTH) domain

UniProt :

Q3UPG6

UniProt :

Q5SUH7

Clone

Cloning Information :

481671

HIP Master Clone ID :

399399

IMAGE ID :

100068127

Original Clone ID :

FLH481671.01X

Organism

Species Specific ID:

216705

Genome

Chromosome :

Map Location :

11 B1.1|11

Ensembl :

ENSMUSG00000006169

Reagents

Labome :

enthoprotin-antibody