Gene:
Gene Symbol:
Dock3
Gene Name:
dedicator of cyto-kinesis 3
Original Clone ID:
FLH481675.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00295428
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 6093nts
Open reading frame: 35 to 6127
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGTGGACCCCCACGGAGGAGGAGAA
ATACGGCGTAGTGATATGCAGCTTTCGAGGATCTGTCCCTCAAGGCCTGGTCTTAGAAAT
AGGAGAAACAGTTCAGATTCTTGAAAAATGTGAAGGTTGGTACAGAGGAGTTTCAACAAA
GAAGCCTAATGTAAAGGGGCTCTTTCCTGCAAATTATATTCATTTGAAAAAGGCAATTGT
CAGTAACAGGGGGCAGTATGAAACTGTGGTTCCGCTGGAAGATTCTATTGTGACTGAAGT
TACAACAACTCTACAAGAGTGGGCCAGTCTGTGGAAACAACTATATGTGAAACACAAAGT
AGATCTTTTCTACAAACTCCGCCATGTGATGAATGAACTTATTGACCTGCGAAGGCAGCT
ACTGTCTGGTCACCTGACACAGGATCAGGTGCGGGAGGTTAAGCGGCACATCACCGTGCG
TCTGGACTGGGGTAATGAGCATTTGGGCCTGGACTTGGTGCCTCGGAAGGACTTTGAAGT
AGTGGATTCAGACCAGATTAGCGTCTCAGATCTCTATAAAATGCATTTATCTAGCCGGCA
GAGTGTACAGCAGAGCACATCCCAAGTAGATACAATGCGCCCAAGGCATGGGGAAACCTG
TCGAATGCCGGTACCACATCATTTCTTCTTCAGCCTGAAGAGTTTCACCTATAATACCAT
TGGTGAAGATAGTGATGTCTTCTTTTCTCTGTATGACATGAGAGAAGGCAAGCAGATCAG
TGAACGGTTTCTGGTGAGACTGAACAAGAATGGTGGGCCAAGGAACCCAGAGAAGATAGA
ACGAATGTGTGCCCTTTTTACAGATCTGAGCAGCAAAGATATGAAGAGAGATTTATACAT
AGTTGCCCATGTGATCCGAATAGGCCGGATGCTCCTGAATGACTCCAAAAAGGGTCCTGC
TCACCTGCACTACAGGCGACCATATGGCTGTGCGGTTCTAAGCATATTGGATGTTCTACA
GTCACTAACAGAATTAAAAGAAGAAAAGGATTTTGTTCTTAAGGTGTACACATGTAACAA
CGAGAGTGAATGGACCCAGATTCATGAGAACATCATCCGAAAGTCAAGTACCAAGTACTC
TGCCCCCAGTGCCAGCCATGGTCTTATTATTTCCCTTCAGCTTTTTCGTGGTGACATGGA
ACAGATCCGGAGAGAAAATCCCATGATATTTAATAGAGGATTAGCAATTACAAGAAAATT
GGGATTTCCTGATGTTATTATGCCAGGTGATATCCGCAATGATTTGTACCTAACACTGGA
GAAGGGAGACTTTGAAAGAGGGGGAAAGAGTGTACAAAAGAATATTGAAGTAACCATGTA
TGTACTTTATGCAGATGGAGAAATCTTGAAGGACTGTATCAGTTTGGGTTCAGGAGAGCC
AAATAGGAGTTCCTATCACTCCTTTGTTCTCTACCACAGTAATAGTCCTCGCTGGGGAGA
AATTATCAAATTACCTATTCCCATTGACAGATTCCGGGGCTCCCATCTCCGCTTTGAGTT
CAGACATTGTTCCACAAAGGACAAAGGGGAAAAGAAACTCTTTGGCTTTGCGTTCTCTCC
GCTGATGCGTGATGATGGCACCACACTCTCAGATGATATTCATGAGCTTTATGTGTATAA
GTGTGATGAGAATAGCACATTTAACAACCATGCCCTCTACCTGGGCCTGCCCTGCTGCAA
AGAAGACTACAATGGCTGCCCTAATATCCCCTCCAGCCTCATCTTCCAGCGCAGCGCCAA
AGAGTCTTTCTTCATTTCTACACAACTCTCTTCTACCAAACTCACACAAAATGTGGACCT
CCTTGCTCTGCTGAAATGGAAGGCCTTCCCAGACCGGATCATGGACATATTAGGGCGGTT
GCGGCATGTCAGTGGGGAAGAAATTGTTAAGTTTCTGCAGGATATCTTAGATACACTCTT
TGTGATTTTGGATGACAATACAGAAAAATACGGCCTTTTGGTTTTTCAGTCTCTGGTTTT
CATTATCAACCTGCTTCGAGACATAAAATACTTCCACTTCCGACCTGTAATGGACACATA
TATCCAGAAGCACTTTGCTGGAGCCCTGGCATATAAGGAGCTCATCCGCTGTTTGAAGTG
GTACATGGACTGCTCAGCAGAACTGATTCGACAGGACCACATTCAGGAAGCCATGCGGGC
CTTGGAGTACCTTTTCAAGTTCATTGTGCAGTCACGAATCCTGTATTCGAGAGCCACATG
TGGGATGGAAGAGGAGCAGTTCCGGTCTAGCATCCAGGAACTTTTCCAGTCCATCCGGTT
TGTGCTGAGTCTGGACAGTAGGAACTCAGAAACACTGCTCTTCACTCAGGCTGCACTACT
CAACTCCTTCCCCACCATCTTCGACGAGCTGCTACAGATGTTTACGGTGCAAGAGGTAGC
AGAGTTTGTGAGAGGCACACTGGGAAGCATGCCTAGCACTGTGCACATCGGGCAGTCAAT
GGATGTGGTGAAGCTGCAGTCCATCGCCAGGACAGTGGATAGTCGCCTATTTTCATTTTC
AGAATCCCGCCGCATCCTACTTCCTGTGGTTCTTCATCACATTCATCTTCACCTGAGGCA
ACAGAAAGAGTTGCTAATCTGCTCAGGGATTCTGGGCAGCATCTTCTCCATCGTCAAGAC
CAGCTCTCTGGAGGCAGATGTCATGGAGGAGGTAGAGATGATGGTGGAAAGCCTCCTGGA
TGTTCTCCTACAGACTCTCCTCACCATCATGAGTAAATCACATGCTCAGGAGGCGGTAAG
AGGGCAGCGGTGCCCGCAGTGCACAGCCGAGATCACTGGTGAATATGTGTCCTGCCTCCT
CTCATTGCTCCGTCAGATGTGTGACACCCATTTCCAACACCTCTTAGACAACTTTCAGAG
TAAAGATGAACTAAAGGAATTTCTGCTGAAGATTTTCTGTGTGTTCAGAAACCTAATGAA
GATGAGTGTCTTCCCTCGAGACTGGATGGTGATGAGACTGTTGACAAGCAACATTATAGT
CACTACTGTCCAGTACCTGTCCTCTGCACTGCATAAGAATTTCACAGAAACAGACTTTGA
CTTCAAGGTGTGGAATTCTTATTTTAGCCTGGCAGTTCTGTTCATAAACCAGCCAAGCCT
TCAGCTAGAAATCATTACTTCAGCCAAGAGGAAGAAGATTCTAGATAAGTATGGGGACAT
GCGTGTGATGATGGCATATGAACTGTTCAGCATGTGGCAGAATCTGGGTGATCATAAGAT
CCACTTTATTCCTGGAATGATTGGTCCTTTCCTGGGTGTGACACTGGTCCCACAGCCAGA
AGTGCGGAATATCATGATTCCTATCTTTCATGACATGATGGACTGGGAACAAAGGAAAAA
TGGCAACTTCAAGCAGGTGGAGGCTGAGCTGATTGACAAGCTGGACAGCATGGTGTCAGA
GGGGAAAGGTGATGAGAGCTACAGGGAACTCTTCGGCCTGCTAACCCAGCTGTTTGGGCC
CTACCCCAGCCTTCTGGAAAAGGTTGAACAGGAAACATGGCGTGAGACTGGCATTTCCTT
TGTTACCTCAGTCACGCGCCTCATGGAACGCCTTCTTGACTACAGGGACTGCATGAAGGG
AGAAGAAACTGAGAACAAGAAGGTTGGCTGCACTGTTAACCTGATGAACTTTTACAAATC
TGAGATTAACAAAGAGGAAATGTATATCCGATACATCCATAAACTTTGTGACATGCACTT
ACAGGCTGAGAACTACACAGAGGCTGCATTCACCCTGCTTCTTTACTGCGAGCTGCTACA
ATGGGAAGACCGGCCATTACGGGAGTTCCTACACTACCCATCCCAAACTGAGTGGCAGCG
GAAAGAGGGACTGTGTAGAAAGATCATCCACTACTTCAACAAAGGCAAGAGCTGGGAGTT
TGGAATTCCTCTATGTAGAGAGCTGGCATGCCAGTACGAGAGCCTCTATGACTACCAGAG
CCTCAGCTGGATCCGGAAAATGGAGGCCAGCTACTATGACAACATCATAGAGCAGCAACG
CCTGGAACCTGAGTTCTTCCGGGTTGGTTTTTATGGCAGGAAGTTTCCTTTCTTCCTTCG
GAACAAAGAATATGTGTGCCGTGGTCATGACTACGAGAGGCTAGAAGCCTTCCAACAGAG
GATGCTGAGCGAGTTCCCACAGGCCGTTGCTATGCAGCACCCCAATCATCCTGATGATGC
CATCCTACAGTGTGATGCTCAGTATTTACAGATCTATGCAGTGACACCTATTCCAGATTA
CGTGGATGTTCTACAAATGGATAGAGTACCTGATCGTGTCAAGAGCTTCTATCGTGTCAA
TAATGTGAGGAAGTTCCGGTATGATAGACCTTTCCACAAAGGCCCCAAGGACAAAGACAA
TGAATTCAAGAGCCTGTGGATTGAGCGCACCACGCTGACACTGACCCACAGTTTGCCTGG
CATCTCTCGGTGGTTTGAAGTGGAAAGAAGGGAACTGGTGGAGGTGAGCCCTCTGGAGAA
TGCAATCCAGGTAGTTGAGAATAAGAACCAGGAACTGCGAGCTCTGATCAGCCAGTATCA
GCACAAGCAGGTACATGGCAACATCAATCTGCTCAGCATGTGCCTGAACGGAGTCATTGA
CGCTGCTGTCAATGGAGGCATTGCACGCTACCAGGAGGCCTTTTTTGATAAAGATTATAT
CACCAAGCACCCAGGAGACGCTGAGAAGATCTCCCAGCTCAAAGAACTAATGCAGGAACA
GGTCCATGTTCTTGGAGTTGGGCTGGCGGTTCATGAGAAGTTTGTGCATCCGGAAATGCG
GCCTCTGCATAAGAAGCTGATTGATCAGTTCCAGATGATGAGGGCCAGTCTGTACCATGA
GTTTCCAGGTTTGGATAAGCTAAGTCCTGCATGTTCAGGCACCAGCACCCCACGGGGAAA
TGTTCTGGCATCCCATAGCCCCATGAGCCCTGAGAACATCAAGATGACCCACAGGCACAG
CCCCATGAACTTGATGGGCACAGGCCGCCATTCCTCATCCTCTCTCTCCTCACATGCATC
TAGTGAAGCAGGAAACATGATGATGATGGGTGACAACTCTATGGGTGAAGCTCCTGAAGA
CCTCTACCACCACATGCAGCTTGCATATCACAACCCCAGGTACCAGGGCTCAGTCACCAA
CGTCTCTGTTCTGTCCTCGTCCCAGGCAAGCCCTTCTTCCTCCAGCCTGAGTTCTACACA
TTCAGCTCCGTCCCAGATGATCACCTCTGCCCCTTCCAGCACCCGAGGCTCTCCCTCTCT
GCCAGATAAGTACCGACATGCCCGGGAAATGATGTTGCTGTTGCCTACACATCGGGACCG
CCCAAGCAGTGCCATGTATCCAGCAGCCATCCTAGAGAATGGACAGCCACCAAACTTCCA
GCGGGCCCTGTTCCAGCAAGTAGTTGGAGCCTGTAAACCCTGCAGTGACCCCAATCTGTC
TATGGCTGAAAAAGGTCATTACTCCCTACACTTCGACGCCTTCCACCACCCTCTGGGTGA
TACCCCCCCGGCCCTCCCTGCCCGGACACTGCGCAAGTCTCCTCTCCACCCCATCCCAGC
CTCCCCTACAAGCCCCCAATCGGGCCTGGATGGCAGCAACTCCACGCTGTCTGGCAGCGC
CAGTAGCGGTGTATCCTCCTTAAGTGAGAGTAACTTTGGGCATTCCTCAGAGGCCCCACC
TCGAACAGACACCATGGACTCCATGCCAAGCCAGGCCTGGAATGGCGATGAAGACCTTGA
GCCGCCCTACCTCCCTGTCCACTACAGCCTCTCTGAATCTGCTGTCCTTGATGCCATCAA
GTCCCAGCCATGCAGGAGCCACTCAGCCCCAGGGTGTGTCCTCCCTCAGGATCCCATGGA
CCCACCTGCGCTGCCACCCAAGCCCTATCACCCCCGCCTGCCAGCCCTAGAGCATGATGA
GGGTATGCTATTGCGGGAGGAGGCTGAGAGGCCAAGGGGCCTGCACCGCAAGGCCTCCCT
GCCTCCTGGAAGTGTCAAGGAGGAACAGGCCCGCCTGGCCTGGGAGCACGGCCGAGGGGA
ACAGTAGGGCCTCATGGGCCCAGCTTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MWTPTEEEKYGVVICSFRGSVPQGLVLEIGETVQILEKCEGWYRGVSTKKPNVKGLFPAN YIHLKKAIVSNRGQYETVVPLEDSIVTEVTTTLQEWASLWKQLYVKHKVDLFYKLRHVMN ELIDLRRQLLSGHLTQDQVREVKRHITVRLDWGNEHLGLDLVPRKDFEVVDSDQISVSDL YKMHLSSRQSVQQSTSQVDTMRPRHGETCRMPVPHHFFFSLKSFTYNTIGEDSDVFFSLY DMREGKQISERFLVRLNKNGGPRNPEKIERMCALFTDLSSKDMKRDLYIVAHVIRIGRML LNDSKKGPAHLHYRRPYGCAVLSILDVLQSLTELKEEKDFVLKVYTCNNESEWTQIHENI IRKSSTKYSAPSASHGLIISLQLFRGDMEQIRRENPMIFNRGLAITRKLGFPDVIMPGDI RNDLYLTLEKGDFERGGKSVQKNIEVTMYVLYADGEILKDCISLGSGEPNRSSYHSFVLY HSNSPRWGEIIKLPIPIDRFRGSHLRFEFRHCSTKDKGEKKLFGFAFSPLMRDDGTTLSD DIHELYVYKCDENSTFNNHALYLGLPCCKEDYNGCPNIPSSLIFQRSAKESFFISTQLSS TKLTQNVDLLALLKWKAFPDRIMDILGRLRHVSGEEIVKFLQDILDTLFVILDDNTEKYG LLVFQSLVFIINLLRDIKYFHFRPVMDTYIQKHFAGALAYKELIRCLKWYMDCSAELIRQ DHIQEAMRALEYLFKFIVQSRILYSRATCGMEEEQFRSSIQELFQSIRFVLSLDSRNSET LLFTQAALLNSFPTIFDELLQMFTVQEVAEFVRGTLGSMPSTVHIGQSMDVVKLQSIART VDSRLFSFSESRRILLPVVLHHIHLHLRQQKELLICSGILGSIFSIVKTSSLEADVMEEV EMMVESLLDVLLQTLLTIMSKSHAQEAVRGQRCPQCTAEITGEYVSCLLSLLRQMCDTHF QHLLDNFQSKDELKEFLLKIFCVFRNLMKMSVFPRDWMVMRLLTSNIIVTTVQYLSSALH KNFTETDFDFKVWNSYFSLAVLFINQPSLQLEIITSAKRKKILDKYGDMRVMMAYELFSM WQNLGDHKIHFIPGMIGPFLGVTLVPQPEVRNIMIPIFHDMMDWEQRKNGNFKQVEAELI DKLDSMVSEGKGDESYRELFGLLTQLFGPYPSLLEKVEQETWRETGISFVTSVTRLMERL LDYRDCMKGEETENKKVGCTVNLMNFYKSEINKEEMYIRYIHKLCDMHLQAENYTEAAFT LLLYCELLQWEDRPLREFLHYPSQTEWQRKEGLCRKIIHYFNKGKSWEFGIPLCRELACQ YESLYDYQSLSWIRKMEASYYDNIIEQQRLEPEFFRVGFYGRKFPFFLRNKEYVCRGHDY ERLEAFQQRMLSEFPQAVAMQHPNHPDDAILQCDAQYLQIYAVTPIPDYVDVLQMDRVPD RVKSFYRVNNVRKFRYDRPFHKGPKDKDNEFKSLWIERTTLTLTHSLPGISRWFEVERRE LVEVSPLENAIQVVENKNQELRALISQYQHKQVHGNINLLSMCLNGVIDAAVNGGIARYQ EAFFDKDYITKHPGDAEKISQLKELMQEQVHVLGVGLAVHEKFVHPEMRPLHKKLIDQFQ MMRASLYHEFPGLDKLSPACSGTSTPRGNVLASHSPMSPENIKMTHRHSPMNLMGTGRHS SSSLSSHASSEAGNMMMMGDNSMGEAPEDLYHHMQLAYHNPRYQGSVTNVSVLSSSQASP SSSSLSSTHSAPSQMITSAPSSTRGSPSLPDKYRHAREMMLLLPTHRDRPSSAMYPAAIL ENGQPPNFQRALFQQVVGACKPCSDPNLSMAEKGHYSLHFDAFHHPLGDTPPALPARTLR KSPLHPIPASPTSPQSGLDGSNSTLSGSASSGVSSLSESNFGHSSEAPPRTDTMDSMPSQ AWNGDEDLEPPYLPVHYSLSESAVLDAIKSQPCRSHSAPGCVLPQDPMDPPALPPKPYHP RLPALEHDEGMLLREEAERPRGLHRKASLPPGSVKEEQARLAWEHGRGEQ*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
402
End on reference sequence
6494
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
