Gene:
Gene Symbol:
Nos1
Gene Name:
nitric oxide synthase 1, neuronal
Original Clone ID:
FLH481679.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00295432
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4290nts
Open reading frame: 35 to 4324
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGGAAGAGCACACGTTTGGGGTCCA
GCAGATCCAACCCAACGTCATTTCTGTCCGTCTCTTCAAACGCAAAGTGGGAGGTCTGGG
CTTCCTGGTGAAGGAACGGGTCAGCAAGCCCCCGGTGATCATCTCAGATCTGATCCGAGG
AGGTGCCGCGGAGCAGAGCGGCCTTATCCAAGCCGGCGACATCATTCTCGCCGTCAATGA
TCGGCCCCTGGTAGACCTCAGCTATGACAGCGCTCTGGAAGTTCTCAGGGGCATTGCCTC
TGAGACCCATGTGGTCCTCATTCTGAGAGGCCCTGAGGGCTTTACTACACACCTGGAGAC
CACCTTCACAGGGGATGGAACCCCCAAGACCATCCGTGTGACCCAGCCCCTGGGGACACC
CACCAAAGCTGTCGATCTGTCTCGCCAGCCATCAGCCAGCAAAGACCAGCCATTAGCAGT
AGACAGGGTCCCAGGTCCCAGTAACGGACCTCAGCATGCCCAAGGCCGTGGACAGGGAGC
CGGCTCAGTCTCCCAGGCTAATGGTGTGGCCATTGACCCCACAATGAAAAATACCAAGGC
CAACCTCCAGGACAGCGGAGAACAGGATGAACTGCTCAAAGAGATAGAACCTGTGTTGAG
CATCCTCACCGGTGGGGGAAAAGCAGTCAACAGAGGGGGACCAGCCAAAGCAGAGATGAA
AGACACAGGAATCCAGGTGGACAGAGACCTCGACGGCAAACTGCACAAAGCTCCGCCCCT
GGGCGGGGAGAATGATCGAGTCTTCAATGATCTGTGGGGGAAGGGCAACGTCCCTGTGGT
CCTCAACAACCCGTATTCAGAGAACGAACAGTCTCCCGCCTCGGGCAAACAGTCTCCTAC
CAAGAATGGCAGTCCTTCCAGGTGCCCTCGCTTCCTTAAGGTCAAGAACTGGGAGACAGA
TGTGGTCCTCACTGACACCCTGCACCTGAAGAGCACGCTGGAAACGGGGTGCACAGAGCA
GATCTGCATGGGCTCCATCATGCTGCCATCCCATCACATACGGAAGTCAGAAGATGTCCG
CACCAAGGACCAGCTCTTCCCTCTAGCCAAAGAATTTCTCGACCAATACTACTCCTCCAT
TAAGAGGTTTGGCTCCAAGGCCCACATGGACAGGCTGGAGGAGGTGAACAAGGAGATCGA
GAGCACCAGCACCTACCAGCTCAAGGACACGGAGCTCATCTATGGCGCCAAGCATGCCTG
GCGGAACGCCTCTCGCTGTGTGGGCAGGATCCAGTGGTCCAAGCTGCAGGTGTTCGATGC
CCGAGACTGCACCACAGCCCATGGGATGTTCAACTACATCTGTAACCACGTCAAGTACGC
CACCAACAAAGGGAACCTCAGGTCGGCCATCACTATATTCCCTCAAAGGACTGATGGCAA
GCATGACTTCCGAGTGTGGAACTCGCAGCTCATCCGCTATGCCGGCTACAAGCAGCCAGA
TGGCTCTACCTTGGGCGATCCAGCTAATGTGGAGTTCACAGAGATCTGTATACAGCAGGG
CTGGAAACCCCCAAGAGGCCGGTTCGATGTGCTGCCTCTCCTGCTCCAGGCCAATGGCAA
TGACCCTGAACTCTTTCAGATCCCCCCAGAGCTGGTGCTGGAAGTACCCATCAGGCACCC
CAAGTTCGACTGGTTTAAGGACCTGGGGCTCAAATGGTATGGCCTCCCCGCTGTGTCCAA
CATGCTGCTGGAGATTGGGGGCCTGGAGTTCAGCGCCTGTCCCTTTAGTGGCTGGTACAT
GGGCACGGAGATTGGCGTTCGTGATTACTGTGACAACTCTCGATACAACATCCTGGAGGA
AGTAGCCAAGAAAATGGATTTGGACATGAGGAAGACATCCTCCCTCTGGAAGGACCAAGC
CCTGGTGGAGATTAACATTGCTGTCCTATACAGCTTCCAGAGTGACAAGGTGACCATTGT
GGACCACCACTCTGCCACCGAGTCCTTCATCAAACACATGGAGAATGAGTACCGCTGCAG
AGGGGGCTGTCCCGCCGACTGGGTGTGGATTGTGCCCCCCATGTCGGGCAGCATCACCCC
TGTCTTCCACCAGGAGATGCTCAACTACCGGCTCACCCCGTCCTTTGAGTACCAGCCTGA
TCCATGGAACACCCACGTGTGGAAGGGCACCAATGGGACCCCCACGAAGCGGCGAGCCAT
TGGCTTCAAGAAATTGGCAGAGGCCGTCAAGTTCTCAGCCAAGCTGATGGGACAGGCCAT
GGCCAAGAGAGTCAAAGCGACCATTCTCTACGCCACCGAGACAGGCAAATCCCAAGCCTA
TGCCAAGACCCTGTGCGAGATCTTCAAGCATGCCTTCGATGCCAAGGCTATGTCCATGGA
GGAATATGACATCGTGCACCTGGAGCACGAAGCCCTGGTCTTGGTGGTCACCAGCACCTT
TGGCAATGGAGACCCCCCAGAGAATGGGGAGAAATTCGGCTGTGCTTTGATGGAGATGAG
GCACCCCAACTCTGTGCAAGAGGAGAGGAAGAGCTACAAGGTCCGATTCAACAGCGTCTC
CTCCTATTCTGACTCCCGCAAGTCATCAGGCGATGGACCAGACCTCAGAGACAACTTTGA
AAGTACTGGACCGCTGGCCAATGTGAGGTTCTCAGTCTTCGGCCTGGGCTCTCGGGCATA
CCCTCACTTCTGTGCCTTTGGGCACGCAGTGGACACTCTCCTGGAGGAGCTGGGCGGGGA
GAGGATTCTGAAGATGAGGGAGGGAGATGAGCTCTGCGGACAGGAAGAGGCTTTCAGGAC
CTGGGCTAAGAAAGTCTTCAAGGCAGCCTGTGATGTGTTCTGTGTGGGGGATGACGTCAA
CATTGAGAAGGCGAACAATTCCCTCATTAGCAATGACCGCAGCTGGAAGAGGAATAAATT
CCGCCTCACTTATGTGGCAGAAGCTCCAGAGCTGACCCAAGGTCTTTCCAATGTTCACAA
AAAGCGAGTCTCAGCCGCCCGACTCCTCAGCCGCCAAAACCTGCAAAGTCCTAAATCCAG
CCGATCGACCATCTTCGTGCGTCTCCACACCAACGGGAATCAGGAGTTGCAGTACCAGCC
AGGGGACCACCTGGGTGTCTTCCCTGGCAACCATGAGGACCTCGTGAATGCACTCATTGA
ACGGCTGGAGGACGCGCCGCCTGCCAACCACGTGGTGAAGGTGGAAATGCTGGAGGAAAG
GAACACGGCTCTGGGTGTCATCAGTAATTGGAAGGATGAATCTCGCCTCCCACCCTGCAC
CATCTTCCAGGCCTTCAAGTACTACCTGGACATCACCACGCCGCCCACGCCCCTGCAGCT
GCAGCAGTTCGCCTCCCTGGCCACCAATGAGAAAGAGAAGCAGCGGCTGCTGGTCCTCAG
CAAGGGGCTTCAGGAATATGAGGAATGGAAATGGGGCAAGAACCCCACAATGGTGGAGGT
GCTGGAGGAGTTCCCGTCCATCCAGATGCCGGCCACACTTCTCCTCACACAGCTGTCGCT
GCTACAACCTCGCTACTATTCCATCAGCTCCTCTCCAGACATGTACCCCGACGAGGTGCA
CCTCACCGTGGCCATCGTGTCCTACCATACCCGAGATGGAGAAGGACCGGTTCACCACGG
GGTGTGCTCCTCCTGGCTCAACCGAATACAGGCTGACGATGTCGTCCCCTGCTTCGTGAG
AGGTGCCCCTAGCTTCCACCTGCCTCGAAACCCCCAGGTGCCTTGCATCCTGGTTGGCCC
AGGCACCGGCATCGCTCCCTTCCGAAGTTTTTGGCAACAGCGACAATTTGACATCCAACA
CAAAGGAATGAATCCGTGCCCCATGGTTCTGGTCTTCGGGTGTCGACAATCCAAGATAGA
TCATATCTACAGAGAGGAGACCCTGCAGGCCAAGAACAAGGGCGTCTTCAGAGAGCTGTA
CACTGCCTACTCCCGGGAACCCGACAGGCCAAAGAAATATGTACAGGACGTGCTGCAGGA
ACAGCTGGCCGAGTCTGTGTACCGCGCCCTGAAGGAGCAAGGAGGCCATATTTATGTCTG
CGGGGACGTTACCATGGCCGCTGATGTCCTCAAAGCCATCCAGCGCATAATGACCCAGCA
GGGGAAACTCTCGGAGGAGGATGCTGGTGTGTTCATCAGCAGGCTGAGGGATGACAACCG
GTACCACGAGGACATCTTTGGGGTCACCCTCAGAACATATGAAGTGACCAACCGCCTTAG
ATCTGAGTCCATCGCCTTCATTGAAGAGAGCAAAAAAGACACAGATGAGGTTTTCAGCTC
CTAGGGCCTCATGGGCCCAGCTTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MEEHTFGVQQIQPNVISVRLFKRKVGGLGFLVKERVSKPPVIISDLIRGGAAEQSGLIQA GDIILAVNDRPLVDLSYDSALEVLRGIASETHVVLILRGPEGFTTHLETTFTGDGTPKTI RVTQPLGTPTKAVDLSRQPSASKDQPLAVDRVPGPSNGPQHAQGRGQGAGSVSQANGVAI DPTMKNTKANLQDSGEQDELLKEIEPVLSILTGGGKAVNRGGPAKAEMKDTGIQVDRDLD GKLHKAPPLGGENDRVFNDLWGKGNVPVVLNNPYSENEQSPASGKQSPTKNGSPSRCPRF LKVKNWETDVVLTDTLHLKSTLETGCTEQICMGSIMLPSHHIRKSEDVRTKDQLFPLAKE FLDQYYSSIKRFGSKAHMDRLEEVNKEIESTSTYQLKDTELIYGAKHAWRNASRCVGRIQ WSKLQVFDARDCTTAHGMFNYICNHVKYATNKGNLRSAITIFPQRTDGKHDFRVWNSQLI RYAGYKQPDGSTLGDPANVEFTEICIQQGWKPPRGRFDVLPLLLQANGNDPELFQIPPEL VLEVPIRHPKFDWFKDLGLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCD NSRYNILEEVAKKMDLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIK HMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHVWKGTN GTPTKRRAIGFKKLAEAVKFSAKLMGQAMAKRVKATILYATETGKSQAYAKTLCEIFKHA FDAKAMSMEEYDIVHLEHEALVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVQEERKS YKVRFNSVSSYSDSRKSSGDGPDLRDNFESTGPLANVRFSVFGLGSRAYPHFCAFGHAVD TLLEELGGERILKMREGDELCGQEEAFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISN DRSWKRNKFRLTYVAEAPELTQGLSNVHKKRVSAARLLSRQNLQSPKSSRSTIFVRLHTN GNQELQYQPGDHLGVFPGNHEDLVNALIERLEDAPPANHVVKVEMLEERNTALGVISNWK DESRLPPCTIFQAFKYYLDITTPPTPLQLQQFASLATNEKEKQRLLVLSKGLQEYEEWKW GKNPTMVEVLEEFPSIQMPATLLLTQLSLLQPRYYSISSSPDMYPDEVHLTVAIVSYHTR DGEGPVHHGVCSSWLNRIQADDVVPCFVRGAPSFHLPRNPQVPCILVGPGTGIAPFRSFW QQRQFDIQHKGMNPCPMVLVFGCRQSKIDHIYREETLQAKNKGVFRELYTAYSREPDRPK KYVQDVLQEQLAESVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLSEEDAGVF ISRLRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDTDEVFSS*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
4388
Start on reference sequence
99
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
