Gene:
Gene Symbol:
Ighmbp2
Gene Name:
immunoglobulin mu binding protein 2
Original Clone ID:
FLH481686.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00295439
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2982nts
Open reading frame: 35 to 3016
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGGCCTCGTCCACCGTGGAGAGTTT
CGTGGCCCAGCAGCTACAGCTGCTGGAGCTGGAGCGGGACGCCGAGGTGGAGGAGCGCAG
GTCCTGGCAGGAACACAGTTCTCTGAGAGAGCTTCAGAGCCGAGGGGTGTGCTTGCTGAA
GCTTCAGGTATCCAGCCAGCGCACAGGGCTGTATGGGCAGCGGCTGGTCACCTTTGAGCC
CAGGAAGTTTGGACCTGCAGTGGTGCTTCCTAGCAACAGCTTCACCTCTGGTGATATTGT
GGGTCTGTATGATACTAATGAAAACAGTCAACTGGCCACTGGGGTCCTGACCCGAATCAC
CCAAAAATCAGTCACAGTGGCTTTTGATGAGTCCCATGATCTCCAGCTGAACCTGGACAG
AGAGAATACCTACAGACTGCTGAAGCTTGCCAACGATGTCACCTACAAACGCCTGAAAAA
AGCACTGATGACACTGAAGAAGTACCACTCTGGGCCAGCATCCTCCCTCATAGACATCCT
GTTGGGCAGCTCCACCCCAAGTCCTGCCATGGAAATACCCCCACTCTCTTTCTACAACAC
AACTCTGGACCTTTCCCAGAAAGAAGCTGTGTCCTTTGCACTGGCCCAGAAAGAACTTGC
CATCATCCATGGGCCTCCTGGCACTGGGAAAACCACAACTGTGGTGGAAATAATCCTTCA
AGCTGTGAAGCAAGGCTTAAAGGTCCTGTGCTGTGCTCCCTCCAACATCGCTGTGGACAA
CCTGGTGGAGCGTCTGGCTCTGTGCAAGAAGCGGATTCTGCGCCTGGGTCACCCCGCCCG
CCTCCTGGAGTCCGTTCAGCACCACTCACTGGATGCAGTGCTAGCACGCAGTGACAACGC
CCAGATTGTTGCTGACATCAGAAGGGACATAGACCAGGTCTTTGGCAAGAACAAAAAGAC
GCAGGATAAGAGAGAGAAAGGTAATTTTCGAAGTGAAATTAAGCTGCTAAGGAAGGAACT
GAAGGAGAGGGAAGAAGCAGCCATAGTTCAGAGCCTCACTGCGGCAGATGTGGTTCTAGC
CACCAACACAGGTGCATCGTCTGATGGCCCTCTGAAGCTGCTGCCTGAGGACTACTTTGA
TGTGGTGGTGGTGGACGAATGTGCCCAGGCCCTAGAAGCCAGCTGCTGGATCCCCCTGCT
GAAGGCCCCAAAGTGCATCCTAGCTGGGGACCACAGACAGCTGCCACCCACCACCGTCTC
TCACAGGGCGGCACTGGCTGGGCTGTCCCGCAGCCTGATGGAGCGTCTGGCAGAGAAGCA
TGGTGCTGGTGTGGTGAGGATGCTGACGGTACAGTACCGAATGCACCAGGCCATCATGTG
CTGGGCCTCGGAGGCCATGTACCACGGGCAGCTTACTTCCCATCCCTCTGTGGCAGGACA
CCTCCTGAAGGACCTCCCAGGCGTGACTGACACAGAGGAGACACGTGTCCCGCTGCTGCT
CATAGACACCGCTGGCTGTGGGCTTCTGGAGCTGGAGGAGGAGGACAGCCAGTCCAAGGG
AAATCCGGGCGAAGTTCGCCTCGTCACTTTGCACATCCAGGCTCTGGTGGATGCTGGGGT
CCAGGCAGGTGACATTGCCGTCATCGCACCCTACAACCTTCAGGTGGATCTGCTCAGACA
GAGCCTCTCCAACAAGCACCCTGAGCTGGAGATTAAGTCTGTTGATGGCTTTCAAGGCCG
AGAGAAAGAGGCTGTGCTCCTGACCTTTGTCAGGTCCAATAGGAAAGGTGAAGTTGGTTT
TCTGGCTGAGGACAGGCGGATTAATGTTGCTGTTACCCGTGCTCGGCGGCACGTGGCAGT
CATCTGTGATTCCCACACTGTCAACAACCATGCTTTTTTGAAGACCTTGGTGGATTATTT
CACAGAGCATGGGGAGGTACGCACAGCCTTTGAATACCTGGATGACATCGTCCCTGAGAA
CTACACCCATGAGGGTTCCCAGGGCCACAGCCGTGTCCCCAAACCCAAGTGCCCCAGCAC
CTCCATCAGGAAGCCTGCCAGTGATCAGGAGAGTGGCCAGGAGACCAGAGCAGCCCCTAG
ACATGGCCGCAGGAAGCCAAGCGAGAAGCCCCCGGGCTCTCACGTCCAGTCCCAGCACAG
CTCCAGTGCAAATGGCTCTGACAGAACTGGAGGCCCAGACCGGACAGAGCACTTTAGGGC
TACGATTGAGGAGTTTGTGGCTAGCAAGGAGTCCCAGTTGGAGTTTCCCACATCCCTGAG
TTCCCATGACAGGCTGCGAGTCCACCAGTTAGCTGAGGAGTTCGGGCTGAGGCACGACAG
CACCGGGGAGGGGAAGGCTCGGCACATCACAGTGAGCAGGAGGAGCCCTGCCAGCTCTGG
CAGTGTAGCCCCACAGCCTTCCTCACCGCCCAGCCCTGCACAGGCTGAGCCTGAGCCTCG
GGCAGAGGAGCCCGTCACCGTCGTGCAGGCACATTGCCCTGTACAGCTGGATCTGAAGGC
ACTGCATCTGGAGAGGCTGCAGCGGCAGCAAAGCTCCCAGGCTCAGACAGCCAAGGGTCA
GCCAGGTGGGGACTCGAGGCCACAGAAGGCTTCACAGAAGAAAAAGAAAAAAGAACCAAA
AGGTCCAGTTATGGCTCTGCCCTGTGAGGAGGACTTCGACGCCCTGGTGTCTGCTGTGGT
GAAGGCTGACAACACCTGTAGCTTCTCCAAGTGCTCGGCCAGCACCACCACTCTGGGCCA
GTTCTGCATGCACTGTAGCCACCGCTACTGCCTCAGCCACCATCTGCCTGAAATCCACGG
CTGTGGAGAAAAGGCTCGTGCCCATGCCCGCCAGAGGATTAGCCGGGAAGGAGTGCTGTA
TGCAGGCAGTGGGACCAAGGACAGGGCCCTGGACCCGGCCAAGAGGGCCCAGCTGCAGAG
GAGGCTGGACAAGAAGCTGGGCGAGCTCAGCAGCCAGAGGACAAGCAGGAAGAAGGAGAA
GGAGAGGGGGACATAGGGCCTCATGGGCCCAGCTTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MASSTVESFVAQQLQLLELERDAEVEERRSWQEHSSLRELQSRGVCLLKLQVSSQRTGLY GQRLVTFEPRKFGPAVVLPSNSFTSGDIVGLYDTNENSQLATGVLTRITQKSVTVAFDES HDLQLNLDRENTYRLLKLANDVTYKRLKKALMTLKKYHSGPASSLIDILLGSSTPSPAME IPPLSFYNTTLDLSQKEAVSFALAQKELAIIHGPPGTGKTTTVVEIILQAVKQGLKVLCC APSNIAVDNLVERLALCKKRILRLGHPARLLESVQHHSLDAVLARSDNAQIVADIRRDID QVFGKNKKTQDKREKGNFRSEIKLLRKELKEREEAAIVQSLTAADVVLATNTGASSDGPL KLLPEDYFDVVVVDECAQALEASCWIPLLKAPKCILAGDHRQLPPTTVSHRAALAGLSRS LMERLAEKHGAGVVRMLTVQYRMHQAIMCWASEAMYHGQLTSHPSVAGHLLKDLPGVTDT EETRVPLLLIDTAGCGLLELEEEDSQSKGNPGEVRLVTLHIQALVDAGVQAGDIAVIAPY NLQVDLLRQSLSNKHPELEIKSVDGFQGREKEAVLLTFVRSNRKGEVGFLAEDRRINVAV TRARRHVAVICDSHTVNNHAFLKTLVDYFTEHGEVRTAFEYLDDIVPENYTHEGSQGHSR VPKPKCPSTSIRKPASDQESGQETRAAPRHGRRKPSEKPPGSHVQSQHSSSANGSDRTGG PDRTEHFRATIEEFVASKESQLEFPTSLSSHDRLRVHQLAEEFGLRHDSTGEGKARHITV SRRSPASSGSVAPQPSSPPSPAQAEPEPRAEEPVTVVQAHCPVQLDLKALHLERLQRQQS SQAQTAKGQPGGDSRPQKASQKKKKKEPKGPVMALPCEEDFDALVSAVVKADNTCSFSKC SASTTTLGQFCMHCSHRYCLSHHLPEIHGCGEKARAHARQRISREGVLYAGSGTKDRALD PAKRAQLQRRLDKKLGELSSQRTSRKKEKERGT*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
225
End on reference sequence
3206
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
