Gene:
Gene Symbol:
Agl
Gene Name:
amylo-1,6-glucosidase, 4-alpha-glucanotransferase
Original Clone ID:
FLH481691.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00295444
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4599nts
Open reading frame: 35 to 4633
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGGAACACAGTAAGCAGATTCGAAT
TTTACTACTGAACGAGATGGAGAAGCTAGAGAAGACCCTCTTCAGACTTGAACAAGGATT
TGAACTACAGTTCCGATTAGGCCCAACTTTACAAGGAAAAGCTGTCACTGTATATACAAA
TTACCCATTGCCTGGAGAAGCATTTAATCGAGAAAAGTTCCGTTCCTTGGATTGGGAAAA
CCCAACGGAGAGAGAAGACGATTCTGATAAATACTGTAAACTTCATCTGCAGCAGTCTGG
ATCTTTTCAGTACTACTTCCTTCAAGGAAATGAGAAAAGTGGAGGAGGTTACATAGTCGT
GGATCCTATTTTACGCATTGGAGTTGATAACCACGTGTTACCCTTGGACTGTGTTACTCT
GCAGACGTTCTTAGCAAAGTGCTTAGGACCCTTCGATGAGTGGGAAAGCCGGCTAAGGGT
GGCAAAGGAGTCAGGTTACAACATGATTCACTTCACCCCACTGCAGACTCTTGGACTGTC
CCGGTCATGCTATTCCCTTGCCGATCAGTTAGAGCTAAATCCTGACTTCTCAAGGCCTAG
TAAAAGATATACCTGGAGTGACGTTGGGCAGCTAGTGGAGAAGCTGAAAAGGGAGTGGAA
TATTCTTTGTATTACCGATGTGGTCTACAATCATACTGCCACTAATAGTAAGTGGATCCT
TGAACACCCGGAATCTGCATACAATCTTGTAAATTCTCCACACTTGAAGCCTGCCTGGGT
CCTAGACAGAGCACTCTGGCATTTCTCCTGTGATGTTGCAGATGGGAAGTACAGAGAAAA
AGGAGTCCCTGCTTTGATTGAAAATGACCAACACATGAATTGCATTCGAAAAATAATTTG
GGAGGACATTTTCCCAAGGATTCAACTCTGGGAGTTTTTCCAAGTAGATGTTCACAAAGC
AGTTGAACAATTTAGAAGACTGCTCTCTCAAGAAAACAGAAGAGTAACTAAGTCTGAACC
AAAAGAACATCTTAAAATTATTCAGGACCCTGAATACAGGCGGCGAGGCTGCGCTGTAGA
TATGGACACTGCTCTGGCGACTTTTATACCACATGACAATGGGCCAGCTGCAATTGAAGA
GTGCTGTAACTGGTTCCGTAAGAGACTGGAGGAGTTAAACTCAGAGAAGCACCACCTCAC
CAGCTGTCACCAGGAACAGGCAGTTAATTGTCTTTTGGGAAACGTGTTTTATGAACGACT
GGCAGGCCATGGCCCTAAACTAGGACCTGTCACCAGGAAGTATCCTTTAGTTACCAGGTA
TTTTACTTTCCCCTTTGGAGAAATGGCCTTATCCGCAGAGGAAGCCCTGATCCACCTCCC
AGATAAAGCGTGCTTCCTGATGGCACACAACGGGTGGGTGATGGGCGATGACCCGCTCCG
GAACTTTGCCGAGCCAGGTTCGGATGTTTATTTAAGGAGAGAACTTATTTGCTGGGGTGA
CAGTGTTAAATTGCGCTATGGGAATAAACCCGAGGACTGCCCTTATCTGTGGGCACACAT
GAAGAAATACACCGAGATAACTGCCACTCACTTCCAGGGAGTTCGTCTGGACAACTGCCA
CTCCACGCCTCTCCACGTGGCTGAGTACATGCTGGATGCTGCTAGGAAATTACAGCCCAA
TCTTTATGTAGTGGCTGAGCTGTTCACAGGAAGCGAAGAGCTGGACAATATCTTCGTTAC
TAGACTGGGCATCAGTTCCTTAATAAGAGAGGCAATGAGCGCGTATAACAGTCACGAAGA
GGGCAGACTGGTCTACCGGTACGGAGGGGAACCTGTCGGCTCCTTCGTTCAGCCCTGTTT
GAGGCCGCTCATGCCTGCTATCGCGCATGCCCTGTTTATGGACATCACCCACGATAATGA
GTGTCCGATTGTGCACAGATCAGCCTATGATGCTCTTCCAAGTACCACAGTTGTTTCCAT
GGCATGCTGTGCCAGTGGAAGCACAAGAGGCTACGACGAGCTAGTGCCTCATCAGATTTC
AGTGGTTGCAGAGGAGCGATTTTACACTAAGTGGAATCCTGGGGCATCACCGGCAGATAC
AGGGGATGTTAATGTCCATAGCGGGATTATTGCAGCCAGATGTGCCATCAATAGGCTCCA
TCAAGAGCTTGGAGCCAAGGGTTTCATTCAAGTGTATGTGGATCAAGTGGATGAAGACAT
AGTGGCTGTAACCAGGCACTCCCCGAGCATCCACCAGTCTGTTGTGGCTGTATCGAGAAC
TGCTTTTAGGAATCCCAAGACTTCCTTTTACAGCAAAGAAGTGCCTCAGATGTGCATCCC
TGGCAAAATTGAAGAGGTAGTTCTGGAAGCTAGAACTATTGAAAGGAACACAAAACCTTA
TAAGAAAGACGAGAACTCGATCAATGGAATGCCGAACATGACAGTGGAACTTAGAGAACA
CATCCAGCTTCATGAAAGTAAAATTGTCAGACAAGCTGGAGTTGCCACAAAGGGTCCCAA
TGAATATATTCAGGAAATAGAATTTGAGAACCTATCTCCAGGAAGTGTTATTATATTCAG
AGTTAGTCTCGATCCACACGCTCAAGTTGCTGTGGGGATTCTTCGGAACCACCTGACCCA
GTTCAGCTCTCACTTTAAGTCCGGAAGCCTTGCTGTTGACAACGCAGATCCTATATTAAA
AATCCCCTTCGCTTCCATTGCCTCTAAATTGACTTTGGCTGAGCTAAACCAGGTACTTTA
CCGATGTGAATCAGAAGAACAAGAAGACGGTGGCGGTTGCTATGACATACCAAACTGGTC
ATCACTTAAGTACGCAGGACTTCAAGGATTAATGTCCGTGTTGGCAGAAATAAGACCGAA
GAATGACCTGGGGCATCCATTCTGTGAAAACTTGAGGTCGGGAGACTGGATGATCGACTA
CGTGAGTGGTCGGCTTATTTCAAGATCAGGAAGTATTGCTGAGGTTGGTAAATGGCTGCA
GGCTATGTTCTTCTACCTGAAGCAGATCCCACGCTACCTCATCCCCTGCTACTTTGACGC
TATATTGATTGGTGCATACACCACTCTGCTGGATGTAGCGTGGAAACAGATGTCAAGCTT
TGTTCAGAACGGATCCACCTTCGTGAAGCACCTGTCACTGGGTTCTGTCCAGATGTGTGG
AGTCGGGAAATGCCCTTGTCTGCCGCTGCTTTCTCCCTCGCTCCTGGATGTGCCGTGCAG
GTTAAATGAGATCACAAAGGAGAAGGAGCAGTGCTGTGCCTCCTTAGCTGCAGGCTTACC
TCACTTTTCTTCCGGTCTTTTTCGCTGCTGGGGGAGGGACACTTTCATTGCCCTTCGAGG
GATGCTGCTGGTTACTGGACGCTACCTGGAAGCCAGGAACATCATTTTAGCGTTTGCTAG
TACCCTGCGGCACGGTCTCATCCCTAACCTCCTCGGCGAAGGGACATATGCCAGGTACAA
CTGCCGGGACGCCGTGTGGTGGTGGCTGCAGTGCATTCAGGACTATTGCAGGACAGTTCC
AAACGGCCTGGACATCCTCAAATGCCCGGTGTCCAGGATGTATCCAACAGATGACTCCGC
ACCCTTGCCTGCTGGGACACTGGACCAGCCTTTGTTTGAAGTCATCCAGGAAGCTATGCA
GAGGCACATGCAGGGCATCCAGTTCCGAGAGAGGAACGCCGGTCCACAGATCGACCGAAA
CATGAAGGACGAGGGTTTCAACATAACTGCGGGGATTGATGAAGAAACAGGATTTGTTTA
TGGAGGAAATCGCTTCAACTGTGGCACGTGGATGGATAAAATGGGAGAGAGTGACCGAGC
TAGGAACAGAGGGATCCCAGCCACTCCAAGAGATGGGTCTGCTGTGGAAATCGTGGGCTT
GTGTAAATCTGCTGTTCGTTGGTTGCTGGAATTGTCTAAGAAAAATATCTTTCCTTATCA
TGAAGTCAGAGTAAAAAGACATGGAAAGGTTGTGGCAGTCTCGTACGATGAGTGGAACAG
AAAAATACAAAACAATTTCGAAAAGCTGTTTCATGTTTCCGAAGACCCTTCAGATCCTAA
CGAGAAGCATCCGAACCTGGTTCACAAACGCGGCATCTACAAGGACAGCTATGGTGCGTC
GAGTCCTTGGTGTGACTATCAGCTCAGGCCCAACTTCACCATAGCAATGGTCGTGGCCCC
TGAACTCTTTACTGCTGAGAAGGCTTGGAAGGCTTTGGAAATTGCAGAAAAAAAATTGCT
TGGTCCCCTTGGCATGAAAACTTTAGACCCAGATGACATGGTTTACTGTGGAGTTTATGA
TAATGCCTTAGACAATGACAACTACAACCTTGCTAGAGGCTTCAATTACCACCAAGGACC
TGAGTGGCTGTGGCCAATTGGGTATTTTCTTCGTGCAAAGTTGTATTTTTCCAAAATGAT
GGGTCCAGAGACTGCTGCGAAGACTGTATTTTTGGTTAAAAATGTTCTTTCTAGACATTA
TGTTCATCTTGAGAGATCCCCATGGAAAGGCCTTCCGGAGCTCACCAATGAGAACGGCCA
GTACTGCCCTTTCAGCTGTGAGACACAGGCGTGGTCAATGGCTGTTGTTCTGGAAACCCT
CTATGATTTATAGGGCCTCATGGGCCCAGCTTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MEHSKQIRILLLNEMEKLEKTLFRLEQGFELQFRLGPTLQGKAVTVYTNYPLPGEAFNRE KFRSLDWENPTEREDDSDKYCKLHLQQSGSFQYYFLQGNEKSGGGYIVVDPILRIGVDNH VLPLDCVTLQTFLAKCLGPFDEWESRLRVAKESGYNMIHFTPLQTLGLSRSCYSLADQLE LNPDFSRPSKRYTWSDVGQLVEKLKREWNILCITDVVYNHTATNSKWILEHPESAYNLVN SPHLKPAWVLDRALWHFSCDVADGKYREKGVPALIENDQHMNCIRKIIWEDIFPRIQLWE FFQVDVHKAVEQFRRLLSQENRRVTKSEPKEHLKIIQDPEYRRRGCAVDMDTALATFIPH DNGPAAIEECCNWFRKRLEELNSEKHHLTSCHQEQAVNCLLGNVFYERLAGHGPKLGPVT RKYPLVTRYFTFPFGEMALSAEEALIHLPDKACFLMAHNGWVMGDDPLRNFAEPGSDVYL RRELICWGDSVKLRYGNKPEDCPYLWAHMKKYTEITATHFQGVRLDNCHSTPLHVAEYML DAARKLQPNLYVVAELFTGSEELDNIFVTRLGISSLIREAMSAYNSHEEGRLVYRYGGEP VGSFVQPCLRPLMPAIAHALFMDITHDNECPIVHRSAYDALPSTTVVSMACCASGSTRGY DELVPHQISVVAEERFYTKWNPGASPADTGDVNVHSGIIAARCAINRLHQELGAKGFIQV YVDQVDEDIVAVTRHSPSIHQSVVAVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEAR TIERNTKPYKKDENSINGMPNMTVELREHIQLHESKIVRQAGVATKGPNEYIQEIEFENL SPGSVIIFRVSLDPHAQVAVGILRNHLTQFSSHFKSGSLAVDNADPILKIPFASIASKLT LAELNQVLYRCESEEQEDGGGCYDIPNWSSLKYAGLQGLMSVLAEIRPKNDLGHPFCENL RSGDWMIDYVSGRLISRSGSIAEVGKWLQAMFFYLKQIPRYLIPCYFDAILIGAYTTLLD VAWKQMSSFVQNGSTFVKHLSLGSVQMCGVGKCPCLPLLSPSLLDVPCRLNEITKEKEQC CASLAAGLPHFSSGLFRCWGRDTFIALRGMLLVTGRYLEARNIILAFASTLRHGLIPNLL GEGTYARYNCRDAVWWWLQCIQDYCRTVPNGLDILKCPVSRMYPTDDSAPLPAGTLDQPL FEVIQEAMQRHMQGIQFRERNAGPQIDRNMKDEGFNITAGIDEETGFVYGGNRFNCGTWM DKMGESDRARNRGIPATPRDGSAVEIVGLCKSAVRWLLELSKKNIFPYHEVRVKRHGKVV AVSYDEWNRKIQNNFEKLFHVSEDPSDPNEKHPNLVHKRGIYKDSYGASSPWCDYQLRPN FTIAMVVAPELFTAEKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGVYDNALDNDNYNLA RGFNYHQGPEWLWPIGYFLRAKLYFSKMMGPETAAKTVFLVKNVLSRHYVHLERSPWKGL PELTNENGQYCPFSCETQAWSMAVVLETLYDL*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
208
End on reference sequence
4806
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
