Gene:
Gene Symbol:
SORBS1
Gene Name:
sorbin and SH3 domain containing 1
Original Clone ID:
FLH481837.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00295495
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGAGTTCTGAATGTGATGGTGGTTCCAAAGCTGTGATGAATGGCTTGGCACCTGGCAGC
AATGGGCAAGACAAAGCAACTGCCGACCCTTTACGCGCACGCTCTATTTCTGCTGTTAAA
ATCATTCCTGTGAAGACAGTGAAAAACGCCTCAGGCCTAGTTCTCCCTACAGACATGGAT
CCTACAAAAATCTGCACTGGGAAGGGAGCGGTGACTCTCCGGGCCTCGTCTTCCTACAGG
GAAACCCCAAGCAGTAGCCCTGCGAGCCCTCAGGAAACCCGGCAACACGAAAGCAAACCA
GGTCTGGAGCCAGAGCCTTCTTCAGCAGATGAGTGGAGGCTTTCTTCCAGTGCTGATGCC
AATGGAAATGCCCAGCCCTCTTCACTCGCTGCCAAGGGCTACAGAAGTGTGCATCCCAAC
CTTCCTTCTGACAAGTCCCAGGATGCCACTTCCTCCAGTGCAGCCCAGCCGGAGGTAATA
GTTGTCCCTCTCTACCTGGTTAATACTGACAGAGGGCAAGAAGGCACTGCCAGACCTCCA
ACACCTCTGGGGCCTCTTGGCTGCGTCCCCACAATCCCAGCGACTGCCTCTGCCGCCTCA
CCTCTGACCTTCCCGACTCTAGATGATTTCATTCCCCCTCATCTGCAGAGGTGGCCCCAC
CACAGCCAGCCAGCCCGCGCCTCTGGCTCCTTTGCCCCCATTAGCCAGACGCCACCATCC
TTCTCACCACCACCTCCGCTGGTCCCTCCTGCCCCGGAGGACCTCCGCAGAGTCTCGGAG
CCTGACCTCACGGGAGCTGTTTCGAGTACCGATTCCAGTCCTCTACTAAATGAAGTTTCT
TCTTCCCTTATTGGAACTGATTCCCAAGCCTTTCCATCAGTTAGCAAGCCTTCATCCGCC
TATCCCTCCACAACGATTGTCAATCCTACTATTGTGCTCTTGCAACACAATCGAGAACAG
CAAAAACGACTCAGTAGCCTTTCAGATCCTGTCTCAGAAAGAAGAGTGGGAGAGCAGGAC
TCAGCACCAACCCAGGAAAAACCCACCTCACCTGGCAAGGCTATTGAAAAAAGAGCAAAG
GATGACAGTAGGCGGGTGGTGAAGAGCACTCAGGACTTAAGCGATGTTTCCATGGATGAA
GTGGGCATCCCACTCCGGAACACTGAGAGATCAAAAGACTGGTACAAGACTATGTTTAAA
CAGATCCACAAACTGAACAGAGACACTCCTGAAGAAAACCCTTATTTCCCTACGTACAAA
TTCCCTGAACTTCCTGAAATCCAGCAAACTTCCGAAGAGGACAATCCTTACACTCCCACC
TACCAGTTTCCTGCATCTACTCCTAGTCCTAAATCTGAAGATGATGATTCAGATCTGTAC
TCTCCCAGATACTCATTTTCTGAAGACACAAAATCTCCCCTTTCTGTGCCTCGCTCAAAA
AGTGAGATGAGCTACATTGATGGTGAGAAGGTAGTCAAGAGGTCGGCCACACTACCCCTC
CCAGCCCGCTCTTCCTCACTGAAGTCAAGCTCAGAAAGAAATGACTGGGAACCCCCAGAT
AAGAAAGTAGACACAAGAAAATATCGTGCAGAGCCCAAGAGCATTTACGAATATCAGCCT
GGCAAGTCTTCCGTTCTGACCAACGAAAAGATGAGTCGGGATATAAGCCCAGAAGAGATA
GATTTAAAGAATGAACCTTGGTATAAATTCTTTTCGGAATTGGAGTTTGGGAAACCGCCT
CCCAAAAAGATATGGGATTATACTCCTGGAGACTGCTCTATCCTTCCTAGAGAGGATAGA
AAGACTAATCTAGACAAAGATCTCAGCCTCTGCCAGACAGAGTTAGAGGCAGATTTAGAA
AAAATGGAGACGCTTAATAAAGCACCCAGTGCAAACGTGCCACAGAGCTCAGCCATCAGC
CCTACTCCGGAAATTTCTTCAGAGACTCCTGGATATATATATTCTTCCAACTTCCATGCA
GTGAAGAGGGAATCAGACGGGGCTCCTGGGGATCTCACTAGCTTGGAGAATGAGAGACAA
ATTTATAAAAGTGTCTTGGAAGGTGGTGACATCCCTCTTCAGGGCCTGAGTGGGCTCAAG
CGACCATCCAGCTCTGCTTCCACTAAAGATTCAGAATCGCCAAGACATTTTATACCAGCT
GATTACTTGGAATCCACGGAAGAATTTATTCGAAGACGTCATGATGATAAAGAGAAACTT
TTAGCGGACCAGAGACGACTTAAACGCGAGCAAGAAGAGGCTGATATTGCAGCTCGACGC
CACACAGGCGTCATTCCGACGCACCATCAGTTTATCACTAATGAGCGCTTTGGGGACCTC
CTCAATATAGACGATACTGCAAAAAGGAAATCTGGGTCAGAGATGAGACCTGCCAGAGCC
AAATTTGACTTTAAAGCTCAGACACTAAAGGAGCTTCCTCTGCAGAAGGGAGATATTGTT
TACATTTATAAGCAAATTGATCAGAACTGGTATGAAGGAGAACACCACGGCCGGGTGGGA
ATCTTCCCACGCACCTACATCGAGCTTCTTCCTCCTGCTGAGAAGGCACAGCCCAAAAAG
TTGACACCAGTGCAGGTTTTGGAATATGGAGAAGCTATTGCTAAGTTTAACTTTAATGGT
GATACACAAGTAGAAATGTCCTTCAGAAAGGGTGAGAGGATCACACTGCTCCGGCAGGTA
GATGAGAACTGGTACGAAGGGAGGATCCCGGGGACATCCCGACAAGGCATCTTCCCCATC
ACCTACGTGGATGTGATCAAGCGACCACTGGTGAAAAACCCTGTGGATTACATGGACCTG
CCTTTCTCCTCCTCCCCAAGTCGCAGTGCCACTGCAAGCCCACAGTTTTCCAGTCACAGC
AAGCTCATCACGCCAGCCCCCTCATCTCTGCCCCACTCCCGCCGAGCCCTGTCCCCCGAG
ATGCACGCTGTCACCTCTGAGTGGATCTCACTGACTGTGGGGGTCCCAGGCAGGCGTTCT
CTGGCCCTGACCCCACCCTTGCCTCCTCTGCCAGAGGCTTCTATCTATAACACTGACCAC
CTCGCCTTGTCACCAAGGGCCAGTCCCTCCCTGTCTCTCAGCCTCCCCCATTTGAGTTGG
TCAGATCGTCCCACCCCACGATCAGTAGCTTCTCCACTGGCCCTACCTTCCCCACACAAA
ACCTACTCCCTAGCACCTACTTCCCAGGCCTCCCTTCACATGAATGGAGACGGTGGTGTC
CACACGCCATCTTCAGGCATCCACCAAGATAGCTTCTTGCAGCTGCCGCTGGGGAGCTCT
GATAGTGTCATCTCCCAGCTTAGTGATGCCTTTAGCAGCCAGAGCAAGAGGCAGCCATGG
CGCGAAGAGAGTGGACAATATGAGAGGAAAGCAGAGAGGGGGGCAGGCGAAAGAGGCCCT
GGTGGACCCAAGATCTCTAAGAAGAGCTGCTTGAAGCCTTCAGACGTGGTCAGGTGCCTG
AGTACTGAACAGAGACTCTCAGATCTCAACACCCCTGAGGAGAGCCGGCCCGGCAAGCCC
CTGGGTAGCGCTTTTCCAGGAAGTGAGGCTGAGCAGACAGAGCGGCATAGAGGTGGCGAG
CAGGCGGGGAGGAAAGCTGCTCGGAGAGGTGGGAGCCAGCAACCTCAAGCCCAGCAGCGA
AGAGTCACCCCCGACAGGAGTCAAACCTCACAAGATTTATTTAGCTATCAAGCATTATAT
AGCTATATACCACAGAATGATGATGAGTTGGAACTCCGCGATGGAGATATCGTTGATGTC
ATGGAAAAATGTGACGATGGATGGTTTGTTGGTACTTCAAGAAGGACAAAGCAGTTTGGT
ACTTTTCCAGGCAACTATGTAAAACCTTTGTATCTA
Protein sequence predicted based on nucleotide sequence
MSSECDGGSKAVMNGLAPGSNGQDKATADPLRARSISAVKIIPVKTVKNASGLVLPTDMD PTKICTGKGAVTLRASSSYRETPSSSPASPQETRQHESKPGLEPEPSSADEWRLSSSADA NGNAQPSSLAAKGYRSVHPNLPSDKSQDATSSSAAQPEVIVVPLYLVNTDRGQEGTARPP TPLGPLGCVPTIPATASAASPLTFPTLDDFIPPHLQRWPHHSQPARASGSFAPISQTPPS FSPPPPLVPPAPEDLRRVSEPDLTGAVSSTDSSPLLNEVSSSLIGTDSQAFPSVSKPSSA YPSTTIVNPTIVLLQHNREQQKRLSSLSDPVSERRVGEQDSAPTQEKPTSPGKAIEKRAK DDSRRVVKSTQDLSDVSMDEVGIPLRNTERSKDWYKTMFKQIHKLNRDTPEENPYFPTYK FPELPEIQQTSEEDNPYTPTYQFPASTPSPKSEDDDSDLYSPRYSFSEDTKSPLSVPRSK SEMSYIDGEKVVKRSATLPLPARSSSLKSSSERNDWEPPDKKVDTRKYRAEPKSIYEYQP GKSSVLTNEKMSRDISPEEIDLKNEPWYKFFSELEFGKPPPKKIWDYTPGDCSILPREDR KTNLDKDLSLCQTELEADLEKMETLNKAPSANVPQSSAISPTPEISSETPGYIYSSNFHA VKRESDGAPGDLTSLENERQIYKSVLEGGDIPLQGLSGLKRPSSSASTKDSESPRHFIPA DYLESTEEFIRRRHDDKEKLLADQRRLKREQEEADIAARRHTGVIPTHHQFITNERFGDL LNIDDTAKRKSGSEMRPARAKFDFKAQTLKELPLQKGDIVYIYKQIDQNWYEGEHHGRVG IFPRTYIELLPPAEKAQPKKLTPVQVLEYGEAIAKFNFNGDTQVEMSFRKGERITLLRQV DENWYEGRIPGTSRQGIFPITYVDVIKRPLVKNPVDYMDLPFSSSPSRSATASPQFSSHS KLITPAPSSLPHSRRALSPEMHAVTSEWISLTVGVPGRRSLALTPPLPPLPEASIYNTDH LALSPRASPSLSLSLPHLSWSDRPTPRSVASPLALPSPHKTYSLAPTSQASLHMNGDGGV HTPSSGIHQDSFLQLPLGSSDSVISQLSDAFSSQSKRQPWREESGQYERKAERGAGERGP GGPKISKKSCLKPSDVVRCLSTEQRLSDLNTPEESRPGKPLGSAFPGSEAEQTERHRGGE QAGRKAARRGGSQQPQAQQRRVTPDRSQTSQDLFSYQALYSYIPQNDDELELRDGDIVDV MEKCDDGWFVGTSRRTKQFGTFPGNYVKPLYL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
3924
Start on reference sequence
46
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
