Gene:
Gene Symbol:
SH3TC1
Gene Name:
SH3 domain and tetratricopeptide repeats 1
Original Clone ID:
FLH481897.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00295555
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGAGAACCTCCCTGCCGTGACCACTGAGGAGCCGACCCCCATGGGGAGGGGTCCTGTG
GGACCCTCAGGAGGTGGCAGCACCCGGGACCAGGTCCGGACTGTGGTCATGAGGCCCTCT
GTGAGCTGGGAGAAAGCGGGGCCCGAGGAGGCCAAGGCGCCAGTGAGAGGCGACGAGGCT
CCTCCTGCCCGCGTGGCTGGGCCTGCTGCTGGGACCCCTCCCTGCCAGATGGGGGTTTAT
CCCACAGACCTGACCCTGCAGCTGCTGGCTGTGCGGAGGAAGAGCAGACTGCGGGACCCC
GGCCTACAGCAGACCCTCCGGGGCCAGCTCCGCCTGCTGGAGAATGATAGCCGGGAGATG
GCCCGCGTGCTTGGGGAATTATCAGCCAGGCTGCTGTCCATCCACAGTGACCAGGACCGG
ATCGTGGTGACGTTTAAGACTTTTGAAGAAATCTGGAAGTTTTCCACCTACCATGCTCTC
GGCTTCACTCATCACTGCCTGGCAAACCTGCTCATGGACCAGGCCTTCTGGCTGCTCTTG
CCCAGTGAGGAGGAGGAGACGGCCATCCAAGTCCATGTGGATGAGAACGCCTTAAGGCTG
ACCCACGAGAGCCTCCTCATCCAAGAAGGGCCCTTCTTTGTCCTGTGTCCTGACCACCAT
GTGAGAGTGATGACGGGTCCCCGGGATGCAGGAAATGGCCCCCAGGCCCTCAGGCAGGCT
TCGGGGGCACCCCAGGGAGAGGCGGCCCCGGAAACAGACTCTTCACCGCCGAGCCCCAGC
GTGTCCTCCGAGGAGGTGGCAGTGGCGGCCGCCCCGGAGCCTTTGATTCCATTTCATCAG
TGGGCTCTTAGGATCCCCCAGGACCCCATCGACGATGCCATGGGTGGCCCTGTGATGCCC
GGCAACCCGCTGATGGCTGTGGGCCTGGCCTCGGCATTGGCAGACTTCCAGGGCTCGGGG
CCCGAAGAGATGACCTTCCGAGGTGGCGACCTCATCGAGATCCTTGGGGCGCAGGTGCCC
AGCCTGCCCTGGTGCGTGGGCCGACACGCAGCCTCGGGCCGGGTGGGGTTTGTGCGGAGC
AGCCTCATCAGCATGCAGGGCCCCGTGTCCGAGTTGGAAAGTGCGATTTTTCTCAATGAG
GAAGAAAAGTCATTCTTCAGCGAGGGCTGCTTTTCTGAGGAGGATGCCAGGCAGTTGCTG
AGGCGGATGTCGGGCACCGATGTCTGCAGCGTGTACAGCCTGGACTCAGTAGAGGAAGCT
GAGACCGAGCAGCCGCAGGAAAAAGAAATACCTCCACCTTGCCTGAGCCCGGAGCCACAG
GAGACCTTGCAGAAGGTGAAGAATGTTCTGGAACAATGCAAGACCTGCCCAGGCTGCCCC
CAGGAGCCAGCGTCCTGGGGTCTCTGTGCGGCATCCAGCGACGTGAGCTTGCAGGACCCC
GAGGAGCCCTCCTTCTGCTTGGAAGCCGAGGACGACTGGGAGGACCCAGAGGCCCTGAGC
TCACTGCTGCTGTTCCTGAACGCCCCTGGGTACAAGGCCAGCTTCCGTGGCCTGTACGAT
GTGGCGCTGCCGTGGCTGAGCAGCGTGTTCCGCAGCTTCAGCGACGAGGAGGAGCTGACT
GGGCGCCTGGCACAGGCCCGGGGGGCGGCCAAGAAAGCTGGCCTCCTCATGGCCCTGGCC
AGGCTCTGCTTCCTCCTGGGGCGGCTGTGCAGCAGGAGGCTCAAGCTGTCCCAGGCCCGG
GTGTACTTTGAGGAAGCGCTGGGGGCCCTGGAGGGCAGCTTCGGGGACCTGTTCCTAGTG
GTGGCTGTGTACGCCAACCTGGCCAGCATTTACCGGAAGCAGAAGAACCGGGAGAAGTGT
GCACAGGTGGTGCCCAAAGCCATGGCCCTGCTCCTGGGGACGCCTGACCACATCTGCAGC
ACCGAGGCGGAGGGGGAGCTCCTGCAGCTGGCGCTGCGGCGGGCGGTGGGTGGCCAGAGC
CTGCAGGCCGAGGCCCGGGCCTGCTTCCTGCTGGCCAGGCACCACGTGCACCTCAAGCAG
CCCGAGGAGGCCCTGCCCTTCCTAGAGCGGCTGCTGCTTTTGCACAGGGACTCGGGAGCC
CCAGAGGCCGCGTGGCTCTCAGACTGCTACCTACTCCTGGCTGACATCTACAGCCGCAAG
TGCCTGCCCCACCTGGTGCTGAGCTGTGTCAAGGTGGCCTCATTGCGGACACGGGGCTCG
CTGGCCGGCTCGCTGAGGAGTGTGAACCTGGTGCTCCAGAACGCCCCCCAGCCCCACAGC
CTCCCTGCCCAAACTTCCCACTACCTCAGGCAAGCGCTGGCCTCCCTGACCCCGGGCACA
GGCCAGGCGCTGCGCGGCCCCCTCTACACCAGCTTGGCCCAGCTGTACAGCCACCATGGC
TGCCACGGCCCGGCCATCACCTTCATGACGCAGGCAGTGGAAGCCAGTGCTATTGCCGGA
GTCCGTGCCATCGTGGACCACCTGGTGGCCCTGGCCTGGCTGCACGTGCTTCATGGGCAG
AGCCCGGTGGCCCTGGACATCCTGCAGTCTGTCCGGGATGCAGTGGTGGCCAGCGAGGAC
CAGGAGGGCGTGATTGCCAACATGGTGGCCGTGGCTCTGAAGAGGACGGGCCGGACGAGG
CAGGCAGCTGAGAGCTACTACCGCGCCCTGCGGGTGGCTCGGGACCTGGGCCAGCAAAGG
AACCAGGCAGTGGGGCTGGCCAACTTCGGGGCCCTGTGCCTGCATGCGGGTGCCAGCAGG
CTGGCCCAGCACTACCTCCTGGAGGCCGTGCGGCTGTTCTCGAGGCTGCCCCTTGGGGAG
TGTGGCCGGGACTTCACCCACGTGCTCCTGCAGCTGGGCCATCTCTGCACCCGCCAGGGC
CCGGCCCAGCAGGGCAAGGGCTACTACGAGTGGGCCCTTCTGGTCGCCGTGGAGATGGGC
CACGTGGAGAGCCAGCTGCGGGCCGTCCAGCGGCTGTGCCACTTCTACAGCGCCGTCATG
CCCAGCGAGGCCCAGTGTGTCATCTACCATGAGCTCCAGCTCTCCCTGGCCTGCAAGGTG
GCCGACAAGGTGCTGGAGGGGCAGCTCCTGGAGACCATCAGCCAGCTCTACCTGTCCCTG
GGCACCGAGCGGGCCTACAAATCCGCACTGGACTACACCAAACGAAGTCTGGGGATTTTC
ATTGACCTCCAGAAGAAAGAGAAGGAGGCGCATGCCTGGCTGCAAGCAGGGAAGATCTAT
TACATCTTGCGGCAGAGCGAGCTGGTGGACCTCTACATCCAGGTGGCACAGAACGTGGCC
CTGTACACAGGCGACCCCAACCTGGGGCTGGAGCTGTTTGAGGCGGCTGGAGACATCTTC
TTCGACGGGGCCTGGGAGCGGGAGAAAGCTGTGTCCTTCTACCGGGACCGGGCCCTGCCC
CTGGCAGTGACTACGGGCAACCGCAAGGCGGAGCTGCGGCTGTGCAACAAGCTGGTGGCA
CTGCTGGCCACGCTGGAGGAGCCCCAGGAGGGCTTGGAGTTTGCCCACATGGCCCTAGCA
CTCAGCATCACCCTGGGGGACCGGCTGAACGAGCGCGTGGCCTACCACCGGCTGGCCGCC
CTGCAACACCGACTGGGCCATGGCGAGCTGGCAGAGCACTTCTACCTCAAGGCCCTGTCG
CTCTGCAACTCGCCGCTGGAGTTTGACGAGGAGACCCTCTACTACGTGAAGGTGTACCTG
GTGCTCGGTGACATCATCTTCTACGACCTGAAGGACCCGTTTGATGCAGCCGGGTACTAC
CAGCTGGCGCTGGCGGCCGCCGTGGACCTGGGCAACAAGAAGGCACAGCTGAAGATCTAC
ACGCGGCTGGCCACCATCTACCACAACTTCCTCCTGGACCGTGAGAAGTCGCTCTTCTTC
TACCAGAAGGCCAGGACCTTCGCCACAGAGCTCAACGTCCGCAGGGTCAACCTGCCTCCT
CTGCCACTCTGCGGGTGGGCCCCCTGGTTGGCCCCCAGCCACCCTCGC
Protein sequence predicted based on nucleotide sequence
MENLPAVTTEEPTPMGRGPVGPSGGGSTRDQVRTVVMRPSVSWEKAGPEEAKAPVRGDEA PPARVAGPAAGTPPCQMGVYPTDLTLQLLAVRRKSRLRDPGLQQTLRGQLRLLENDSREM ARVLGELSARLLSIHSDQDRIVVTFKTFEEIWKFSTYHALGFTHHCLANLLMDQAFWLLL PSEEEETAIQVHVDENALRLTHESLLIQEGPFFVLCPDHHVRVMTGPRDAGNGPQALRQA SGAPQGEAAPETDSSPPSPSVSSEEVAVAAAPEPLIPFHQWALRIPQDPIDDAMGGPVMP GNPLMAVGLASALADFQGSGPEEMTFRGGDLIEILGAQVPSLPWCVGRHAASGRVGFVRS SLISMQGPVSELESAIFLNEEEKSFFSEGCFSEEDARQLLRRMSGTDVCSVYSLDSVEEA ETEQPQEKEIPPPCLSPEPQETLQKVKNVLEQCKTCPGCPQEPASWGLCAASSDVSLQDP EEPSFCLEAEDDWEDPEALSSLLLFLNAPGYKASFRGLYDVALPWLSSVFRSFSDEEELT GRLAQARGAAKKAGLLMALARLCFLLGRLCSRRLKLSQARVYFEEALGALEGSFGDLFLV VAVYANLASIYRKQKNREKCAQVVPKAMALLLGTPDHICSTEAEGELLQLALRRAVGGQS LQAEARACFLLARHHVHLKQPEEALPFLERLLLLHRDSGAPEAAWLSDCYLLLADIYSRK CLPHLVLSCVKVASLRTRGSLAGSLRSVNLVLQNAPQPHSLPAQTSHYLRQALASLTPGT GQALRGPLYTSLAQLYSHHGCHGPAITFMTQAVEASAIAGVRAIVDHLVALAWLHVLHGQ SPVALDILQSVRDAVVASEDQEGVIANMVAVALKRTGRTRQAAESYYRALRVARDLGQQR NQAVGLANFGALCLHAGASRLAQHYLLEAVRLFSRLPLGECGRDFTHVLLQLGHLCTRQG PAQQGKGYYEWALLVAVEMGHVESQLRAVQRLCHFYSAVMPSEAQCVIYHELQLSLACKV ADKVLEGQLLETISQLYLSLGTERAYKSALDYTKRSLGIFIDLQKKEKEAHAWLQAGKIY YILRQSELVDLYIQVAQNVALYTGDPNLGLELFEAAGDIFFDGAWEREKAVSFYRDRALP LAVTTGNRKAELRLCNKLVALLATLEEPQEGLEFAHMALALSITLGDRLNERVAYHRLAA LQHRLGHGELAEHFYLKALSLCNSPLEFDEETLYYVKVYLVLGDIIFYDLKDPFDAAGYY QLALAAAVDLGNKKAQLKIYTRLATIYHNFLLDREKSLFFYQKARTFATELNVRRVNLPP LPLCGWAPWLAPSHPR
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
102
End on reference sequence
4112
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
