Gene:
Gene Symbol:
GIGYF2
Gene Name:
GRB10 interacting GYF protein 2
Original Clone ID:
FLH482305.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00295963
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGCAGCGGAAACGCAGACACTGAACTTTGGGCCTGAATGGCTCCGAGCTCTGTCCAGT
GGTGGGAGTATTACATCCCCTCCTCTTTCTCCAGCATTGCCGAAGTATAAATTAGCAGAT
TATCGTTACGGCAGAGAAGAAATGTTAGCACTTTTCCTTAAAGACAACAAGATACCTTCA
GACCTTCTGGATAAAGAATTTCTGCCTATCCTCCAGGAGGAACCCCTTCCACCATTGGCT
CTGGTACCCTTTACAGAAGAAGAACAGAGAAACTTTTCCATGTCTGTAAATAGTGCTGCT
GTCCTGCGATTGACAGGACGAGGAGGAGGAGGAACAGTGGTGGGGGCTCCTAGAGGTCGA
AGTTCTTCAAGAGGGCGAGGCAGAGGCAGAGGTGAATGTGGTTTCTACCAAAGAAGTTTT
GATGAAGTAGAGGGTGTTTTTGGTCGAGGAGGTGGCAGAGAAATGCATAGATCGCAGAGC
TGGGAGGAAAGGGGTGACAGACGTTTTGAAAAACCAGGACGAAAAGATGTAGGGAGACCA
AATTTTGAGGAAGGTGGACCAACATCAGTAGGGAGAAAGCATGAATTTATACGCTCAGAA
AGTGAAAATTGGCGCATCTTTAGAGAGGAACAAAATGGAGAAGATGAAGATGGAGGTTGG
CGACTAGCTGGATCAAGGAGGGATGGAGTGAGGTGGCGACCTCACAGTCCTGGCTGGCGG
GAACACATGGAACGACGTCGGAGGTTTGAGTTTGATTTTCGAGATAGAGATGATGAACGG
GGTTACCGAAGGGTTCGCTCTGGCAGTGGGAGCATAGATGATGACAGGGATAGCTTGCCC
GAATGGTGCTTAGAGGATGCTGAAGAAGAAATGGGTACATTTGACTCATCTGGAGCATTC
CTTTCTCTAAAAAAAGTACAGAAAGAGCCTATTCCAGAAGAGCAGGAGATGGACTTCCGG
CCTGTGGACGAAGGGGAGGAGTGCTCTGACTCTGAGGGTAGCCATAATGAAGAGGCCAAA
GAACCCGATAAGACAAATAAGAAAGAAGGAGAGAAAACAGATAGAGTAGGAGTTGAAGCT
AGTGAGGAAACTCCCCAGACCTCATCATCATCTGCTAGACCAGGTACTCCTTCAGACCAT
CAGTCTCAGGAAGCATCACAGTTTGAGAGGAAAGATGAACCAAAAACTGAGCAAACGGAA
AAAGCTGAAGAGGAGACTCGGATGGAAAATAGTCTACCAGCCAAAGTGCCCAGCAGAGGG
GATGAAATGGTTGCTGATGTCCAGCAGCCCCTGTCGCAGATTCCCTCAGATACAGCCTCT
CCTCTTCTCATACTTCCACCTCCTGTTCCCAATCCTAGTCCTACTCTCCGGCCAGTTGAA
ACACCAGTTGTAGGTGCTCCTGGTATGGGCAGTGTTTCCACAGAACCTGATGATGAAGAA
GGTCTCAAACATTTGGAGCAGCAAGCTGAGAAAATGGTGGCTTATCTCCAAGACAGTGCA
CTAGATGATGAAAGATTGGCATCAAAACTGCAAGAGCACAGAGCTAAAGGAGTGTCGATT
CCATTGATGCATGAAGCAATGCAGAAGTGGTATTACAAAGATCCTCAGGGAGAAATTCAA
GGTCCCTTCAATAATCAGGAGATGGCAGAATGGTTTCAGGCGGGCTATTTTACTACGTCT
TTATTGGTGAAGAGAGCGTGTGATGAAAGCTTCCAACCTCTTGGCGATATCATGAAAATG
TGGGGAAGGGTTCCCTTTTCTCCAGGTCCAGCTCCCCCTCCTCATATGGGAGAGCTGGAC
CAGGAACGACTGACCAGGCAGCAAGAACTCACAGCCTTATACCAGATGCAGCACCTGCAG
TACCAGCAGTTTTTAATACAACAACAATATGCACAGGTTTTGGCCCAACAGCAGAAAGCA
GCACTGTCTTCCCAGCAGCAGCAGCAGTTGGCACTTCTTCTTCAACAGTTTCAGACCTTG
AAGATGAGAATATCTGATCAGAACATCATTCCCTCAGTAACTAGGTCTGTGTCCGTGCCA
GATACTGGCTCTATCTGGGAGCTTCAGCCAACAGCTTCACAGCCTACAGTTTGGGAAGGT
GGTAGTGTATGGGATCTTCCTCTGGACACCACGACACCAGGCCCTGCCCTGGAACAGCTT
CAGCAGCTAGAGAAGGCCAAAGCTGCAAAGCTAGAGCAAGAGAGAAGAGAGGCAGAAATG
AGGGCAAAACGGGAAGAGGAAGAGCGAAAGAGGCAGGAAGAACTCCGAAGACAACAGGAG
GAAATTCTTCGGCGACAGCAGGAAGAAGAAAGGAAAAGGCGAGAGGAAGAAGAACTTGCC
CGAAGGAAACAGGAAGAGGCTCTGCGTCGCCAGCGGGAGCAAGAAATTGCATTAAGGCGA
CAGCGAGAAGAGGAAGAAAGACAGCAGCAAGAAGAAGCTCTTAGAAGACTGGAAGAGAGG
AGAAGAGAAGAGGAAGAAAGGCGGAAGCAGGAAGAATTGTTACGCAAACAGGAAGAGGAG
GCTGCAAAATGGGCCCGGGAAGAAGAAGAAGCCCAGCGTCGATTAGAGGAGAACCGGCTG
CGGATGGAAGAGGAGGCAGCCAGACTCCGGCATGAGGAAGAAGAACGGAAGAGAAAGGAG
CTGGAGGTCCAGCGGCAGAAGGAGTTAATGCGCCAGAGGCAGCAGCAGCAAGAGGCTCTC
CGGAGGTTGCAGCAGCAGCAGCAGCAACAACAGCTGGCGCAGATGAAGCTTCCTTCTTCT
TCAACGTGGGGCCAGCAGTCCAATACAACAGCATGTCAGTCCCAGGCCACGCTGTCGTTG
GCTGAAATCCAAAAACTAGAGGAAGAACGAGAACGGCAGCTTCGAGAAGAGCAAAGGCGC
CAGCAGAGGGAGTTGATGAAAGCTCTTCAGCAGCAGCAGCAGCAGCAACAGCAGAAACTC
TCAGGTTGGGGGAATGTCAGCAAACCTTCAGGTACCACGAAATCTCTTCTGGAGATCCAG
CAGGAAGAGGCCAGGCAAATGCAAAAGCAGCAGCAGCAGCAGCAGCAACACCAGCAACCA
AACAGAGCTCGTAACAATACGCATTCCAACCTGCACACCAGCATTGGGAATTCTGTTTGG
GGCTCTATAAATACTGGTCCTCCTAACCAGTGGGCATCTGACCTAGTCAGTAGTATTTGG
AGTAATGCTGACACTAAAAACTCCAACATGGGATTCTGGGATGATGCAGTGAAAGAGGTG
GGACCTAGGAATTCAACAAATAAAAATAAAAACAACGCCAGTCTCAGTAAATCTGTAGGT
GTGTCTAACCGGCAGAATAAGAAAGTAGAAGAAGAAGAAAAGTTGCTGAAGCTCTTTCAG
GGAGTAAATAAAGCCCAAGATGGATTTACGCAGTGGTGTGAACAGATGCTTCATGCCCTT
AATACGGCAAATAACTTGGATGTTCCCACATTTGTTTCTTTCCTGAAAGAAGTAGAATCT
CCTTATGAGGTCCATGATTATATCAGGGCCTATTTAGGAGATACTTCTGAGGCCAAGGAG
TTTGCCAAGCAGTTCCTTGAGCGTCGTGCCAAACAGAAAGCCAACCAGCAGCGTCAGCAG
CAGCAGCAGCCGCCACAGCAGCCGCCACAGCAGCCACAACAGCAGGACTCTGTGTGGGGG
ATGAACCACAGTACACTCCATTCAGTATTTCAGACCAATCAAAGCAACAACCAACAATCC
AATTTTGAGGCTGTGCAGAGTGGCAAGAAGAAGAAAAAGCAGAAGATGGTCCGAGCAGAT
CCCAGTTTATTAGGATTTTCAGTCAATGCATCATCGGAGCGACTCAACATGGGTGAAATC
GAGACGTTGGATGACTAC
Protein sequence predicted based on nucleotide sequence
MAAETQTLNFGPEWLRALSSGGSITSPPLSPALPKYKLADYRYGREEMLALFLKDNKIPS DLLDKEFLPILQEEPLPPLALVPFTEEEQRNFSMSVNSAAVLRLTGRGGGGTVVGAPRGR SSSRGRGRGRGECGFYQRSFDEVEGVFGRGGGREMHRSQSWEERGDRRFEKPGRKDVGRP NFEEGGPTSVGRKHEFIRSESENWRIFREEQNGEDEDGGWRLAGSRRDGVRWRPHSPGWR EHMERRRRFEFDFRDRDDERGYRRVRSGSGSIDDDRDSLPEWCLEDAEEEMGTFDSSGAF LSLKKVQKEPIPEEQEMDFRPVDEGEECSDSEGSHNEEAKEPDKTNKKEGEKTDRVGVEA SEETPQTSSSSARPGTPSDHQSQEASQFERKDEPKTEQTEKAEEETRMENSLPAKVPSRG DEMVADVQQPLSQIPSDTASPLLILPPPVPNPSPTLRPVETPVVGAPGMGSVSTEPDDEE GLKHLEQQAEKMVAYLQDSALDDERLASKLQEHRAKGVSIPLMHEAMQKWYYKDPQGEIQ GPFNNQEMAEWFQAGYFTTSLLVKRACDESFQPLGDIMKMWGRVPFSPGPAPPPHMGELD QERLTRQQELTALYQMQHLQYQQFLIQQQYAQVLAQQQKAALSSQQQQQLALLLQQFQTL KMRISDQNIIPSVTRSVSVPDTGSIWELQPTASQPTVWEGGSVWDLPLDTTTPGPALEQL QQLEKAKAAKLEQERREAEMRAKREEEERKRQEELRRQQEEILRRQQEEERKRREEEELA RRKQEEALRRQREQEIALRRQREEEERQQQEEALRRLEERRREEEERRKQEELLRKQEEE AAKWAREEEEAQRRLEENRLRMEEEAARLRHEEEERKRKELEVQRQKELMRQRQQQQEAL RRLQQQQQQQQLAQMKLPSSSTWGQQSNTTACQSQATLSLAEIQKLEEERERQLREEQRR QQRELMKALQQQQQQQQQKLSGWGNVSKPSGTTKSLLEIQQEEARQMQKQQQQQQQHQQP NRARNNTHSNLHTSIGNSVWGSINTGPPNQWASDLVSSIWSNADTKNSNMGFWDDAVKEV GPRNSTNKNKNNASLSKSVGVSNRQNKKVEEEEKLLKLFQGVNKAQDGFTQWCEQMLHAL NTANNLDVPTFVSFLKEVESPYEVHDYIRAYLGDTSEAKEFAKQFLERRAKQKANQQRQQ QQQPPQQPPQQPQQQDSVWGMNHSTLHSVFQTNQSNNQQSNFEAVQSGKKKKKQKMVRAD PSLLGFSVNASSERLNMGEIETLDDY
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
23
End on reference sequence
3883
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
