Gene:
Gene Symbol:
IARS
Gene Name:
isoleucyl-tRNA synthetase
Original Clone ID:
FLH482375.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00296033
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
GTACAAAAAAGCAGGCTCCACCATGCTTCAACAAGTTCCAGAAAACATAAATTTTCCTGC
TGAGGAAGAGAAAATCTTGGAGTTTTGGACTGAATTTAATTGTTTTCAGGAATGCTTAAA
GCAATCAAAACATAAACCAAAATTTACCTTCTATGATGGTCCTCCTTTTGCAACTGGACT
GCCTCACTATGGACATATACTTGCGGGTACAATTAAAGATATAGTTACAAGATATGCTCA
CCAGAGTGGGTTTCATGTTGACAGAAGATTTGGATGGGATTGCCATGGCTTACCTGTGGA
ATATGAAATTGATAAGACACTGGGAATCAGAGGACCAGAGGATGTGGCCAAAATGGGGAT
TACAGAGTATAACAATCAGTGCCGAGCAATTGTGATGAGATATTCTGCTGAGTGGAAGTC
TACTGTTAGCAGACTTGGCCGATGGATTGACTTTGACAATGACTATAAAACTCTGTATCC
ACAATTCATGGAATCAGTCTGGTGGGTCTTCAAACAACTCTATGATAAAGGCCTTGTTTA
TAGAGGTGTGAAAGTCATGCCCTTCTCTACGGCATGTAACACTCCACTTTCCAACTTCGA
GTCACACCAGAATTATAAGGATGTTCAAGATCCTTCAGTATTTGTAACTTTCCCTTTGGA
AGAAGATGAAACTGTATCTTTAGTTGCTTGGACAACCACTCCCTGGACTCTACCTAGTAA
CCTTGCTGTGTGTGTTAATCCAGAAATGCAATATGTGAAAATTAAAGATGTTGCCAGAGG
ACGATTACTCATTTTAATGGAAGCCAGATTGTCAGCCCTCTATAAATTGGAGAGTGACTA
TGAGATCCTTGAAAGATTTCCTGGTGCCTATCTTAAAGGCAAGAAGTACAGGCCCCTGTT
TGACTATTTCCTGAAGTGTAAAGAGAATGGCGCTTTCACTGTGCTTGTTGACAACTATGT
GAAGGAAGAAGAAGGCACAGGGGTTGTCCACCAAGCTCCTTACTTCGGTGCTGAGGACTA
TCGGGTCTGTATGGACTTTAACATTATTCGGAAAGACTCACTCCCTGTTTGCCCTGTGGA
TGCTTCAGGCTGCTTCACAACGGAGGTGACAGATTTCGCAGGACAGTATGTGAAGGATGC
TGACAAAAGTATCATCAGGACTTTGAAGGAACAAGGCCGACTTCTGGTTGCCACCACCTT
CACTCACAGCTACCCTTTTTGCTGGAGATCAGACACTCCTCTAATTTACAAAGCAGTGCC
CAGCTGGTTTGTGCGAGTGGAGAACATGGTGGACCAGCTCCTAAGGAACAATGACCTGTG
CTACTGGGTCCCAGAGTTGGTACGAGAAAAACGATTTGGAAATTGGCTGAAAGATGCACG
TGACTGGACAATTTCCAGAAACAGATACTGGGGCACCCCCATCCCACTGTGGGTCAGCGA
TGACTTTGAGGAGGTGGTATGCATTGGGTCAGTGGCGGAACTTGAAGAACTGTCAGGAGC
AAAGATCTCAGATCTCCACAGAGAGAGTGTTGACCACCTGACCATTCCTTCACGCTGTGG
GAAGGGATCCTTGCACCGCATCTCTGAAGTGTTTGACTGTTGGTTTGAGAGTGGCAGCAT
GCCCTATGCTCAGGTTCATTACCCGTTTGAAAACAAGAGGGAGTTTGAGGATGCTTTTCC
TGCAGATTTCATTGCCGAGGGCATCGACCAAAGCAGAGGATGGTTTTATACCCTGCTGGT
GCTGGCCACGGCCCTCTTTGGACAACCGCCTTTCAAGAACGTAATTGTGAATGGGCTTGT
CCTGGCAAGTGATGGCCAAAAAATGAGCAAACGGAAAAAGAATTATCCAGATCCAGTTTC
CATCATCCAGAAGTATGGTGCTGATGCCCTCAGATTATATCTGATTAACTCCCCTGTGGT
GAGAGCAGAAAACCTCCGCTTTAAAGAAGAGGGTGTGCGGGACGTCCTTAAGGATGTACT
GCTCCCATGGTACAATGCCTATCGCTTCTTAATCCAGAACGTTCTGAGGCTCCAGAAGGA
GGAAGAAATAGAATTTCTCTACAATGAGAACACGGTTAGAGAAAGCCCCAACATTACAGA
CCGGTGGATCCTGTCCTTCATGCAGTCTCTCATTGGCTTCTTTGAGACTGAAATGGCAGC
TTATAGGCTTTATACTGTGGTGCCTCGCCTGGTCAAGTTTGTAGATATTCTGACCAATTG
GTATGTTAGAATGAACCGCAGAAGATTAAAGGGTGAAAATGGGATGGAGGATTGTGTCAT
GGCCCTAGAAACCTTGTTTAGTGTTCTGCTTTCTCTTTGCAGACTTATGGCTCCCTACAC
ACCTTTTCTCACTGAATTGATGTACCAGAATCTAAAGGTGCTGATTGACCCTGTTTCTGT
TCAGGACAAGGACACACTCAGCATTCACTACCTCATGCTGCCCCGTGTTCGAGAAGAATT
GATTGACAAGAAAACAGAGAGTGCAGTATCTCAGATGCAGTCTGTGATTGAACTTGGAAG
AGTGATCAGAGACCGAAAAACTATTCCCATAAAGTATCCTTTGAAAGAAATTGTGGTTAT
CCATCAAGATCCAGAAGCTCTTAAAGATATCAAGTCTTTGGAGAAGTATATCATTGAGGA
ACTCAATGTTCGAAAAGTTACACTGTCTACAGATAAAAACAAGTATGGCATTCGGCTAAG
GGCAGAACCAGATCACATGGTCCTGGGGAAGCGTCTGAAGGGAGCCTTTAAGGCAGTGAT
GACGTCCATCAAGCAGTTGAGCAGTGAGGAGCTGGAGCAGTTCCAGAAGACTGGGACCAT
TGTTGTGGAAGGCCATGAATTGCACGATGAAGACATCCGCCTCATGTACACCTTTGATCA
GGCCACAGGTGGGACTGCGCAATTTGAAGCACACTCAGATGCTCAGGCTTTGGTCCTCTT
AGATGTCACTCCTGACCAGTCAATGGTAGATGAAGGAATGGCTCGGGAAGTCATCAATCG
CATACAGAAACTTCGCAAAAAGTGCAATCTGGTTCCAACTGATGAAATCACAGTGTACTA
TAAAGCAAAGTCTGAAGGAACATATCTGAATAGTGTTATTGAAAGCCACACAGAGTTCAT
ATTTACCACCATAAAGGCTCCCTTGAAACCATATCCAGTTTCTCCATCGGATAAAGTCCT
TATTCAAGAAAAAACACAGTTGAAGGGATCTGAACTGGAAATTACACTCACCAGAGGATC
TTCCCTTCCTGGTCCTGCTTGTGCATATGTCAATCTTAACATTTGTGCAAATGGCAGTGA
ACAAGGTGGAGTATTGCTCCTGGAAAATCCAAAAGGTGACAATAGGTTGGACCTTTTAAA
GCTGAAGAGTGTTGTCACTAGCATTTTTGGTGTGAAAAATACAGAGCTGGCTGTCTTCCA
TGATGAAACAGAAATACAAAACCAAACTGACTTACTGAGTCTTAGTGGAAAAACACTTTG
TGTGACTGCAGGATCGGCTCCCTCTCTGATCAACAGTTCTAGTACTCTTCTTTGTCAGTA
TATCAACCTACAGCTCCTGAATGCAAAGCCACAAGAGTGTTTAATGGGGACAGTGGGCAC
TCTCCTGCTTGAAAACCCACTTGGGCAGAATGGACTCACCCACCAAGGTCTTCTGTATGA
AGCAGCCAAGGTGTTTGGCCTTCGGAGCAGGAAGCTAAAGCTGTTTCTGAATGAGACCCA
AACGCAGGAAATTACAGAAGACATCCCCGTGAAGACTTTGAATATGAAGACTGTGTATGT
TTCTGTGTTACCAACAACAGCAGACTTCTGAATCCACCCAGCTTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MLQQVPENINFPAEEEKILEFWTEFNCFQECLKQSKHKPKFTFYDGPPFATGLPHYGHIL AGTIKDIVTRYAHQSGFHVDRRFGWDCHGLPVEYEIDKTLGIRGPEDVAKMGITEYNNQC RAIVMRYSAEWKSTVSRLGRWIDFDNDYKTLYPQFMESVWWVFKQLYDKGLVYRGVKVMP FSTACNTPLSNFESHQNYKDVQDPSVFVTFPLEEDETVSLVAWTTTPWTLPSNLAVCVNP EMQYVKIKDVARGRLLILMEARLSALYKLESDYEILERFPGAYLKGKKYRPLFDYFLKCK ENGAFTVLVDNYVKEEEGTGVVHQAPYFGAEDYRVCMDFNIIRKDSLPVCPVDASGCFTT EVTDFAGQYVKDADKSIIRTLKEQGRLLVATTFTHSYPFCWRSDTPLIYKAVPSWFVRVE NMVDQLLRNNDLCYWVPELVREKRFGNWLKDARDWTISRNRYWGTPIPLWVSDDFEEVVC IGSVAELEELSGAKISDLHRESVDHLTIPSRCGKGSLHRISEVFDCWFESGSMPYAQVHY PFENKREFEDAFPADFIAEGIDQSRGWFYTLLVLATALFGQPPFKNVIVNGLVLASDGQK MSKRKKNYPDPVSIIQKYGADALRLYLINSPVVRAENLRFKEEGVRDVLKDVLLPWYNAY RFLIQNVLRLQKEEEIEFLYNENTVRESPNITDRWILSFMQSLIGFFETEMAAYRLYTVV PRLVKFVDILTNWYVRMNRRRLKGENGMEDCVMALETLFSVLLSLCRLMAPYTPFLTELM YQNLKVLIDPVSVQDKDTLSIHYLMLPRVREELIDKKTESAVSQMQSVIELGRVIRDRKT IPIKYPLKEIVVIHQDPEALKDIKSLEKYIIEELNVRKVTLSTDKNKYGIRLRAEPDHMV LGKRLKGAFKAVMTSIKQLSSEELEQFQKTGTIVVEGHELHDEDIRLMYTFDQATGGTAQ FEAHSDAQALVLLDVTPDQSMVDEGMAREVINRIQKLRKKCNLVPTDEITVYYKAKSEGT YLNSVIESHTEFIFTTIKAPLKPYPVSPSDKVLIQEKTQLKGSELEITLTRGSSLPGPAC AYVNLNICANGSEQGGVLLLENPKGDNRLDLLKLKSVVTSIFGVKNTELAVFHDETEIQN QTDLLSLSGKTLCVTAGSAPSLINSSSTLLCQYINLQLLNAKPQECLMGTVGTLLLENPL GQNGLTHQGLLYEAAKVFGLRSRKLKLFLNETQTQEITEDIPVKTLNMKTVYVSVLPTTA DF*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
3869
Start on reference sequence
81
Mutations:
None
Discrepancy:
a42g; c1691g,T564S; g3612a
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
