Gene:
Gene Symbol:
ANKMY1
Gene Name:
ankyrin repeat and MYND domain containing 1
Original Clone ID:
FLH482700.01L
Keyword:
STOP codon removed based on OC annotation
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00296358
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGAAGGGGCCCATGCCTCCCTTAGCTTAGAGGACGAAGTCTCTGGGGCTGGCAGCCGC
CAACGCCCGCTAGAGGGGAAGGGCGGCGAGACCCCTGCTGCCGAGGAGCCGGGGTCCCTG
AAGAACTACGCTGTCTTCGCCACAAGGGATGTTTCAGCAGCCCCTGAGAAGGAAGAGGAG
GAAGCTGAGGGCCCGCTGAGGGCGCAGGACCTGAGGGAGTCCTACATCCAGCTCGTCCAG
GGTGTGCAGGAGTGGCAGGATGGTTGCATGTACCAGGGGGAGTTTGGGTTGAACATGAAG
CTTGGATATGGCAAATTCTCTTGGCCCACAGGCGAGTCATACCATGGGCAGTTTTACCGG
GACCACTGCCATGGCCTGGGTACCTACATGTGGCCAGATGGCTCCAGTTTCACGGGCACA
TTTTACCTCAGCCACCGAGAAGGCTACGGCACCATGTACATGAAGACACGGCTTTTCCAG
ACTCACTGCCACAACGACATTGTCAACCTTCTCCTGGACTGTGGGGCCGACGTGAACAAG
TGCTCAGATGAGGGTCTCACGGCACTCAGCATGTGTTTCCTCCTCCACTACCCCGCCCAG
TCCTTCAAGCCCAATGTTGCTGAACGGACCATACCTGAGCCCCAGGAACCTCCAAAATTC
CCAGTTGTTCCAATCCTTTCATCATCATTTATGGACACAAACCTGGAGTCTCTGTACTAT
GAGGTGAACGTGCCTTCCCAGGGTAGCTATGAGCTGAGGCCACCGCCAGCACCACTGCTC
CTGCCACGCGTCTCAGGCAGCCACGAGGGCGGCCACTTCCAGGACACCGGGCAGTGTGGG
GGGTCCATAGACCACAGGAGCAGCTCTCTGAAGGGGGACTCCCCGTTGGTGAAGGGCAGC
CTTGGCCATGTGGAAAGCGGGCTTGAGGACGTGTTGGGAAACACAGACCGGGGCAGTCTG
TGCAGTGCTGAGACGAAATTTGAGTCCAACGTGTGTGTGTGCGACTTCTCCATCGAGCTC
TCGCAGGCCATGCTGGAGAGAAGCGCCCAGTCCCACAGCTTGCTGAAGATGGCCTCGCCC
TCACCGTGCACCAGCAGCTTCGACAAAGGGACCATGCGGAGGATGGCGCTGTCCATGATC
GAGCGGAGGAAGCGCTGGCGGACCATCAAGCTGCTGCTGCGCCGGGGCGCGGACCCCAAC
CTGTGCTGCGTGCCCATGCAGGTCCTGTTCCTTGCTGTGAAGGCCGGGGACGTGGATGGG
GTGAGGCTGCTGCTGGAGCACGGGGCGAGGACCGACATCTGCTTTCCGCCGCAGCTGAGC
ACCCTGACACCACTCCACATCGCTGCCGCCCTTCCTGGGGAGGAGGGGGTACAGATTGTG
GAGCTGCTGTTGCATGCCATCACCGATGTGGACGCCAAGGCATCCGACGAGGACGACACT
TACAAGCCCGGCAAGCTGGACCTGCTGCCCTCAAGTCTGAAGCTCAGCAATGAGCCAGGC
CCTCCCCAAGCCTACTACAGCACGGACACAGCCCTCCCGGAGGAGGGGGGCAGGACGGCT
CTGCACATGGCCTGCGAGCGGGAGGATGACAACAAGATTGACCGACTCATCAGTCACGGG
GCCGACATCCTGAAGCCTGTAATGCTCAGGCAGGGAGAAAAGGAGGCAGTGGGCACAGCC
GTGGACTATGGCTACTTCAGATTCTTCCAGGACCGGAGGATTGCCCGCTGCCCCTTCCAC
ACGCTGATGCCAGCAGAGCGCGAGACGTTCCTGGCGCGGAAGCGGCTCCTGGAGTACATG
GGCTTGCAGCTACGGCAGGCTGTCTTTGCCAAGGAGAGCCAGTGGGACCCCACGTGGCTG
TACCTGTGCAAGAGAGCGGAGCTGATCCCCAGCCACAGGATGAAGAAGAAGGGCCCCAGC
CTGCCCAGGGGCCTGGATGTGAAGGAGCAGGGGCAAATTCCCTTCTTCAAGTTCTGCTAC
CAGTGTGGCCGCTCCATCGGGGTCCGCCTCTTGCCCTGCCCTCGCTGCTACGGGATCCTG
ACCTGCAGCAAGTACTGCAAGACCAAGGCCTGGACCGAGTTCCACAAGAAGGACTGCGGG
GACCTGGTGGCCATCGTGACACAACTGGAGCAAGTTTCCAGGAGGAGAGAAGAATTCCAG
Protein sequence predicted based on nucleotide sequence
MEGAHASLSLEDEVSGAGSRQRPLEGKGGETPAAEEPGSLKNYAVFATRDVSAAPEKEEE EAEGPLRAQDLRESYIQLVQGVQEWQDGCMYQGEFGLNMKLGYGKFSWPTGESYHGQFYR DHCHGLGTYMWPDGSSFTGTFYLSHREGYGTMYMKTRLFQTHCHNDIVNLLLDCGADVNK CSDEGLTALSMCFLLHYPAQSFKPNVAERTIPEPQEPPKFPVVPILSSSFMDTNLESLYY EVNVPSQGSYELRPPPAPLLLPRVSGSHEGGHFQDTGQCGGSIDHRSSSLKGDSPLVKGS LGHVESGLEDVLGNTDRGSLCSAETKFESNVCVCDFSIELSQAMLERSAQSHSLLKMASP SPCTSSFDKGTMRRMALSMIERRKRWRTIKLLLRRGADPNLCCVPMQVLFLAVKAGDVDG VRLLLEHGARTDICFPPQLSTLTPLHIAAALPGEEGVQIVELLLHAITDVDAKASDEDDT YKPGKLDLLPSSLKLSNEPGPPQAYYSTDTALPEEGGRTALHMACEREDDNKIDRLISHG ADILKPVMLRQGEKEAVGTAVDYGYFRFFQDRRIARCPFHTLMPAERETFLARKRLLEYM GLQLRQAVFAKESQWDPTWLYLCKRAELIPSHRMKKKGPSLPRGLDVKEQGQIPFFKFCY QCGRSIGVRLLPCPRCYGILTCSKYCKTKAWTEFHKKDCGDLVAIVTQLEQVSRRREEFQ
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
100
End on reference sequence
2262
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
