Gene:
Gene Symbol:
RAC1
Gene Name: 
ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)
Original Clone ID:
FLH482931.01L
Keyword:
STOP codon removed based on OC annotation
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00296589
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 576nts
Open reading frame: 1 to 576
ATGCAGGCCATCAAGTGTGTGGTGGTGGGAGACGGAGCTGTAGGTAAAACTTGCCTACTG
ATCAGTTACACAACCAATGCATTTCCTGGAGAATATATCCCTACTGTCTTTGACAATTAT
TCTGCCAATGTTATGGTAGATGGAAAACCGGTGAATCTGGGCTTATGGGATACAGCTGGA
CAAGAAGATTATGACAGATTACGCCCCCTATCCTATCCGCAAACAGATGTGTTCTTAATT
TGCTTTTCCCTTGTGAGTCCTGCATCATTTGAAAATGTCCGTGCAAAGTGGTATCCTGAG
GTGCGGCACCACTGTCCCAACACTCCCATCATCCTAGTGGGAACTAAACTTGATCTTAGG
GATGATAAAGACACGATCGAGAAACTGAAGGAGAAGAAGCTGACTCCCATCACCTATCCG
CAGGGTCTAGCCATGGCTAAGGAGATTGGTGCTGTAAAATACCTGGAGTGCTCGGCGCTC
ACACAGCGAGGCCTCAAGACAGTGTTTGACGAAGCGATCCGAGCAGTCCTCTGCCCGCCT
CCCGTGAAGAAGAGGAAGAGAAAATGCCTGCTGTTG
Protein sequence predicted based on nucleotide sequence
MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAG QEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCPNTPIILVGTKLDLR DDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQRGLKTVFDEAIRAVLCPP PVKKRKRKCLLL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
204
End on reference sequence
782
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
Gene
Gene Symbol :
RAC1
Symbol Nomenclature :
RAC1
SYNONYM :
Rac-1; TC-25; p21-Rac1
Designation :
cell migration-inducing gene 5 protein|ras-like protein TC25|ras-related C3 botulinum toxin substrate 1
Full Nomenclature :
ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)
GENEID :
Locus Tag :
MIG5
GI :
GenBank Accession :
HGNC :
MIM :
Vega :
Target GenBank:
Pathways
NCI :
Alpha4 beta1 integrin signaling events
NCI :
Alpha9 beta1 integrin signaling events
NCI :
Angiopoietin receptor Tie2-mediated signaling
NCI :
Arf1 pathway
NCI :
Arf6 downstream pathway
NCI :
BCR signaling pathway
NCI :
CDC42 signaling events
NCI :
Class I PI3K signaling events
NCI :
CXCR4-mediated signaling events
NCI :
E-cadherin signaling in keratinocytes
NCI :
E-cadherin signaling in the nascent adherens junction
NCI :
Endothelins
NCI :
EPHA2 forward signaling
NCI :
EPHB forward signaling
NCI :
Ephrin B reverse signaling
NCI :
ErbB1 downstream signaling
NCI :
ErbB2/ErbB3 signaling events
NCI :
IL2 signaling events mediated by PI3K
NCI :
IL6-mediated signaling events
NCI :
Integrin-linked kinase signaling
NCI :
Integrins in angiogenesis
NCI :
Lissencephaly gene (LIS1) in neuronal migration and development
NCI :
LPA receptor mediated events
NCI :
mTOR signaling pathway
NCI :
N-cadherin signaling events
NCI :
Nectin adhesion pathway
NCI :
Nephrin/Neph1 signaling in the kidney podocyte
NCI :
Netrin-mediated signaling events
NCI :
Neurotrophic factor-mediated Trk receptor signaling
NCI :
Noncanonical Wnt signaling pathway
NCI :
Nongenotropic Androgen signaling
NCI :
Osteopontin-mediated events
NCI :
p38 MAPK signaling pathway
NCI :
p75(NTR)-mediated signaling
NCI :
PDGFR-beta signaling pathway
NCI :
Posttranslational regulation of adherens junction stability and dissassembly
NCI :
RAC1 signaling pathway
NCI :
Regulation of p38-alpha and p38-beta
NCI :
Regulation of RAC1 activity
NCI :
S1P1 pathway
NCI :
S1P2 pathway
NCI :
S1P3 pathway
NCI :
Signaling events mediated by focal adhesion kinase
NCI :
Signaling events mediated by Hepatocyte Growth Factor Receptor (c-Met)
NCI :
Signaling events mediated by PRL
NCI :
Signaling events regulated by Ret tyrosine kinase
NCI :
Syndecan-4-mediated signaling events
NCI :
Thromboxane A2 receptor signaling
NCI :
Urokinase-type plasminogen activator (uPA) and uPAR-mediated signaling
Panther :
Axon guidance mediated by netrin
Panther :
Axon guidance mediated by semaphorins
Panther :
Axon guidance mediated by Slit/Robo
Panther :
B cell activation
Panther :
Cytoskeletal regulation by Rho GTPase
Panther :
EGF receptor signaling pathway
Panther :
FGF signaling pathway
Panther :
Huntington disease
Panther :
Inflammation mediated by chemokine and cytokine signaling pathway
Panther :
Integrin signalling pathway
Panther :
Ras Pathway
Panther :
T cell activation
Panther :
VEGF signaling pathway
Reactome :
Activation of Rac
Reactome :
Adaptive Immune System
Reactome :
Axon guidance
Reactome :
beta-catenin independent WNT signaling
Reactome :
CD28 co-stimulation
Reactome :
CD28 dependent Vav1 pathway
Reactome :
Cell death signalling via NRAGE, NRIF and NADE
Reactome :
Cell-Cell communication
Reactome :
Costimulation by the CD28 family
Reactome :
DAP12 interactions
Reactome :
DAP12 signaling
Reactome :
DCC mediated attractive signaling
Reactome :
Developmental Biology
Reactome :
Disease
Reactome :
DSCAM interactions
Reactome :
Factors involved in megakaryocyte development and platelet production
Reactome :
Fc epsilon receptor (FCERI) signaling
Reactome :
FCERI mediated MAPK activation
Reactome :
Fcgamma receptor (FCGR) dependent phagocytosis
Reactome :
G alpha (12/13) signalling events
Reactome :
GPCR downstream signaling
Reactome :
GPVI-mediated activation cascade
Reactome :
Hemostasis
Reactome :
HIV Infection
Reactome :
Host Interactions of HIV factors
Reactome :
Immune System
Reactome :
Inactivation of Cdc42 and Rac
Reactome :
Innate Immune System
Reactome :
L1CAM interactions
Reactome :
Membrane Trafficking
Reactome :
Nef and signal transduction
Reactome :
Netrin-1 signaling
Reactome :
NRAGE signals death through JNK
Reactome :
p75 NTR receptor-mediated signalling
Reactome :
PCP/CE pathway
Reactome :
Platelet activation, signaling and aggregation
Reactome :
Regulation of actin dynamics for phagocytic cup formation
Reactome :
Sema3A PAK dependent Axon repulsion
Reactome :
SEMA3A-Plexin repulsion signaling by inhibiting Integrin adhesion
Reactome :
Sema4D in semaphorin signaling
Reactome :
Sema4D induced cell migration and growth-cone collapse
Reactome :
Sema4D mediated inhibition of cell attachment and migration
Reactome :
Semaphorin interactions
Reactome :
Signal Transduction
Reactome :
Signal transduction by L1
Reactome :
Signaling by GPCR
Reactome :
Signaling by Robo receptor
Reactome :
Signaling by SCF-KIT
Reactome :
Signaling by Wnt
Reactome :
Signalling by NGF
Reactome :
The role of Nef in HIV-1 replication and disease pathogenesis
Reactome :
Translocation of GLUT4 to the plasma membrane
WikiPathway :
Alpha 6 Beta 4 signaling pathway
WikiPathway :
Androgen receptor signaling pathway
WikiPathway :
angiogenesis overview
WikiPathway :
B Cell Receptor Signaling Pathway
WikiPathway :
DNA damage response (only ATM dependent)
WikiPathway :
EGF/EGFR Signaling Pathway
WikiPathway :
Focal Adhesion
WikiPathway :
G13 Signaling Pathway
WikiPathway :
IL-6 signaling pathway
WikiPathway :
Insulin Signaling
WikiPathway :
Integrated Breast Cancer Pathway
WikiPathway :
Integrin-mediated cell adhesion
WikiPathway :
Leptin signaling pathway
WikiPathway :
MAPK signaling pathway
WikiPathway :
MicroRNAs in cardiomyocyte hypertrophy
WikiPathway :
NOD pathway
WikiPathway :
p38 MAPK Signaling Pathway
WikiPathway :
Physiological and Pathological Hypertrophy of the Heart
WikiPathway :
Prolactin Signaling Pathway
WikiPathway :
RANKL/RANK Signaling Pathway
WikiPathway :
Regulation of Actin Cytoskeleton
WikiPathway :
Regulation of toll-like receptor signaling pathway
WikiPathway :
TGF beta Signaling Pathway
WikiPathway :
TNF alpha Signaling Pathway
WikiPathway :
Toll-like receptor signaling pathway
WikiPathway :
TOR signaling
WikiPathway :
TWEAK Signaling Pathway
WikiPathway :
Wnt Signaling Pathway
Protein
SMART domain :
SMART domain :
SMART domain :
UniProt :
UniProt :
UniProt :
HPRD :
03627
Clone
Cloning Information :
HIP Master Clone ID :
400659
IMAGE ID :
100067746
Original Clone ID :
FLH482931.01L
Organism
Species Specific ID:
Genome
Reagents
Labome :