Gene:
Gene Symbol:
MYRF
Gene Name:
myelin regulatory factor
Original Clone ID:
FLH269731.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00297059
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGCACTGGCTTCCAGCAGGCCACGACATCAACGGTGCCCTGGAGCCCTCCAACATAGAC
ACCAGCATCCTGGAGGAGTACATCAGCAAGGAGGATGCCTCCGACCTCTGCTTCCCTGAC
ATCTCTGCTCCAGCCAGCTCGGCCTCCTACTCCCACGGGCAGCCTGCGATGCCTGGCTCC
AGCGGGGTCCACCACCTGAGCCCCCCTGGGGGTGGACCCTCCCCGGGGCGCCATGGTCCC
CTCCCACCCCCGGGCTACGGCACCCCGCTGAACTGCAACAACAACAACGGCATGGGCGCT
GCCCCCAAGCCCTTCCCGGGGGGCACCGGGCCCCCCATCAAGGCTGAGCCCAAGGCTCCC
TATGCCCCAGGCACACTGCCGGACTCTCCCCCAGACTCGGGCTCCGAGGCCTACTCCCCC
CAGCAGGTGAATGAGCCCCACCTCCTGCGCACGATAACCCCTGAGACACTGTGCCACGTG
GGAGTGCCCTCCCGCCTGGAGCATCCGCCCCCACCTCCAGCCCACTTGCCAGGCCCCCCG
CCACCCCCACCACCCCCACCTCACTACCCTGTCCTGCAGCGGGATCTGTACATGAAGGCC
GAGCCCCCGATCCCCCACTACGCTGCCATGGGGCAGGGGCTGGTGCCCACTGATCTTCAC
CACACCCAGCAGTCCCAGATGCTGCACCAGCTCCTGCAGCAGCACGGAGCTGAGCTCCCT
ACACACCCCTCCAAGAAGAGGAAGCACTCTGAATCCCCCCCGAGCACCCTCAATGCCCAG
ATGCTGAATGGAATGATCAAACAGGAGCCTGGGACCGTGACAGCCCTGCCTCTGCACCCC
ACTCGAGCCCCATCGCCACCCTGGCCTCCCCAGGGTCCGCTCTCCCCGGGCCCTGGTTCC
TTGCCTCTCAGCATTGCCCGTGTCCAGACACCGCCTTGGCACCCGCCAGGTGCCCCCTCC
CCAGGCCTCCTGCAGGACAGTGACAGCCTCAGTGGCTCCTACCTGGACCCCAACTACCAG
TCCATCAAGTGGCAGCCTCATCAGCAGAACAAGTGGGCGACCCTGTACGATGCTAACTAC
AAGGAGCTGCCCATGCTCACCTACCGCGTGGATGCGGACAAGGGCTTCAACTTTTCGGTG
GGCGACGACGCCTTTGTGTGCCAGAAGAAGAACCACTTCCAGGTGACAGTGTACATCGGC
ATGCTGGGCGAGCCCAAGTACGTCAAGACGCCCGAGGGCCTCAAGCCCCTCGACTGCTTC
TATCTGAAGCTGCACGGAGTGAAGCTGGAGGCCCTGAACCAGTCCATTAACATCGAGCAG
TCCCAGTCAGACCGGAGCAAGCGGCCCTTCAACCCGGTCACGGTCAATCTGCCCCCTGAG
CAGGTCACGAAGGTGACTGTGGGGCGGCTGCACTTCAGCGAGACCACCGCTAACAACATG
CGTAAGAAGGGCAAGCCCAACCCGGACCAGAGGTACTTCATGCTGGTGGTGGCCCTCCAG
GCTCATGCACAGAACCAGAACTACACGCTGGCCGCCCAGATCTCAGAGCGCATCATTGTG
CGGGCCTCCAACCCAGGCCAGTTCGAGAGCGACAGCGATGTGTTGTGGCAGCGGGCACAG
GTGCCCGACACCGTCTTCCACCACGGCCGCGTGGGCATCAACACAGACCGGCCGGATGAG
GCGCTGGTTGTGCACGGGAATGTCAAGGTCATGGGCTCGCTTATGCACCCCTCCGACCTG
CGCGCCAAGGAACACGTGCAGGAGGTGGACACCACCGAGCAATTGAAGAGGATCTCGCGC
ATGCGGCTGGTGCACTACAGATACAAGCCCGAGTTCGCCGCCAGCGCGGGCATCGAGGCC
ACCGCGCCAGAGACAGGTGTCATCGCTCAGGAGGTGAAGGAGATCTTGCCTGAGGCTGTG
AAAGACACCGGAGACATGGTCTTTGCCAATGGGAAAACCATAGAGAACTTCCTGGTGGTG
AACAAGGAGCGCATCTTCATGGAGAACGTAGGGGCCGTGAAGGAGCTGTGCAAGCTGACA
GACAACCTGGAGACGCGCATTGATGAGCTGGAGCGCTGGAGCCACAAGCTGGCCAAGCTG
CGGCGGCTCGACAGCCTCAAGTCCACCGGCAGCTCGGGCGCCTTCAGCCATGCAGGGAGC
CAGTTCAGTCGGGCGGGCAGCGTCCCCCACAAGAAGAGGCCCCCCAAGGTGGCCAGCAAG
TCATCGTCCGTGGTTCCGGACCAGGCCTGCATCAGCCAGCGCTTCCTGCAGGGAACCATC
ATTGCCCTGGTGGTGGTCATGGCCTTCAGCGTGGTGTCCATGTCCACACTGTACGTGCTG
AGCCTGCGCACAGAGGAGGACCTGGTAGACACTGATGGCAGGTCCAGCCAGAGCTTTGGG
ACCACGCAGCTCCGACAGTCCCCCTTGACCACGGGGCTACCAGGCATACAGCCCTCTTTG
CTGCTGGTGACCACCAGCCTCACCAGCTCGGCCCCAGGTTCTGCTGTCCGCACCTTGGAC
ATGTGTTCCAGCCACCCCTGCCCTGTCATCTGCTGTTCCTCACCCACTACCAACCCTACC
ACTGGTCCTAGTCTTGGCCCCAGCTTTAACCCTGGCCATGTTCTCAGCCCAAGTCCCAGC
CCCAGCACCAACCGCTCAGGCCCCAGCCAGATGGCCCTTCTGCCAGTCACCAACATCAGA
GCCAAGTCCTGGGGTCTTTCAGTCAATGGCATTGGCCACTCCAAGCATCACAAGAGTCTG
GAGCCTCTGGCCAGCCCTGCAGTCCCCTTCCCTGGGGGGCAGGGCAAAGCCAAGAACAGT
CCCAGCCTTGGTTTCCATGGCCGGGCCCGCCGAGGGGCCCTCCAGTCCAGCGTGGGCCCT
GCTGAGCCCACCTGGGCCCAGGGCCAGTCAGAGCCAGTGCCCTCCCTGACCTCCATCCAG
GTGCTGGAGAATTCGATGTCCATCACCTCCCAGTACTGTGCTCCAGGGGATGCCTGCAGG
CCTGGGAACTTCACCTACCACATCCCTGTCAGTAGTGGCACCCCACTGCACCTCAGCCTG
ACTCTGCAGATGAACTCCTCCTCCCCCGTGTCTGTGGTGCTGTGCAGCCTGAGGTCAAAG
GAGGAACCATGTGAGGAGGGGAGCCTTCCACAGAGTCTCCACACCCACCAGGACACCCAG
GGCACCTCTCACCGGTGGCCAATAACCATCCTGTCCTTCCGTGAATTCACCTACCACTTC
CGGGTGGCACTGCTGGGTCAGGCCAACTGCAGTTCAGAGGCTCTCGCCCAGCCAGCCACA
GACTACCACTTCCACTTCTACCGCCTGTGTGAC
Protein sequence predicted based on nucleotide sequence
MHWLPAGHDINGALEPSNIDTSILEEYISKEDASDLCFPDISAPASSASYSHGQPAMPGS SGVHHLSPPGGGPSPGRHGPLPPPGYGTPLNCNNNNGMGAAPKPFPGGTGPPIKAEPKAP YAPGTLPDSPPDSGSEAYSPQQVNEPHLLRTITPETLCHVGVPSRLEHPPPPPAHLPGPP PPPPPPPHYPVLQRDLYMKAEPPIPHYAAMGQGLVPTDLHHTQQSQMLHQLLQQHGAELP THPSKKRKHSESPPSTLNAQMLNGMIKQEPGTVTALPLHPTRAPSPPWPPQGPLSPGPGS LPLSIARVQTPPWHPPGAPSPGLLQDSDSLSGSYLDPNYQSIKWQPHQQNKWATLYDANY KELPMLTYRVDADKGFNFSVGDDAFVCQKKNHFQVTVYIGMLGEPKYVKTPEGLKPLDCF YLKLHGVKLEALNQSINIEQSQSDRSKRPFNPVTVNLPPEQVTKVTVGRLHFSETTANNM RKKGKPNPDQRYFMLVVALQAHAQNQNYTLAAQISERIIVRASNPGQFESDSDVLWQRAQ VPDTVFHHGRVGINTDRPDEALVVHGNVKVMGSLMHPSDLRAKEHVQEVDTTEQLKRISR MRLVHYRYKPEFAASAGIEATAPETGVIAQEVKEILPEAVKDTGDMVFANGKTIENFLVV NKERIFMENVGAVKELCKLTDNLETRIDELERWSHKLAKLRRLDSLKSTGSSGAFSHAGS QFSRAGSVPHKKRPPKVASKSSSVVPDQACISQRFLQGTIIALVVVMAFSVVSMSTLYVL SLRTEEDLVDTDGRSSQSFGTTQLRQSPLTTGLPGIQPSLLLVTTSLTSSAPGSAVRTLD MCSSHPCPVICCSSPTTNPTTGPSLGPSFNPGHVLSPSPSPSTNRSGPSQMALLPVTNIR AKSWGLSVNGIGHSKHHKSLEPLASPAVPFPGGQGKAKNSPSLGFHGRARRGALQSSVGP AEPTWAQGQSEPVPSLTSIQVLENSMSITSQYCAPGDACRPGNFTYHIPVSSGTPLHLSL TLQMNSSSPVSVVLCSLRSKEEPCEEGSLPQSLHTHQDTQGTSHRWPITILSFREFTYHF RVALLGQANCSSEALAQPATDYHFHFYRLCD
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
3371
Start on reference sequence
36
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
